FastQCFastQC Report
Mon 16 Jan 2017
ERR1146630_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146630_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233126
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.035.2875141
GTATCAA98950.034.453731
CAACGCA91350.034.2195175
ATCAACG93550.033.2658773
AACGCAG105800.030.2460656
TATCAAC112900.028.9801142
TCAACGC108550.028.7979494
ACGCAGA115200.027.0032467
CGCAGAG119300.025.9173078
GTACATG209600.023.780921
TACATGG209550.022.859742
ACATGGG205600.022.270023
GTACTAG29950.020.0968131
CATGGGG158850.019.9453854
GCAGAGT158000.019.092579
GTATAAG38450.017.6108461
ATGGGGG105350.017.4909045
GTCGCCG15450.017.36888794
GAGTACA161200.017.2983131
CTAGTAC39500.017.2566183
Produced by FastQC (version 0.11.5)