##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146630_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7233126 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20515555791507 31.0 31.0 34.0 28.0 34.0 2 31.503502773213132 31.0 31.0 34.0 28.0 34.0 3 31.608529424207457 33.0 31.0 34.0 28.0 34.0 4 35.17319634691833 37.0 35.0 37.0 32.0 37.0 5 35.132205079795376 37.0 35.0 37.0 32.0 37.0 6 35.265491573076424 37.0 35.0 37.0 32.0 37.0 7 35.20818205019517 37.0 35.0 37.0 32.0 37.0 8 35.19968627672185 37.0 35.0 37.0 32.0 37.0 9 36.866237225785916 39.0 37.0 39.0 33.0 39.0 10-11 36.84663152556723 39.0 37.0 39.0 33.0 39.0 12-13 36.79477600694361 39.0 37.0 39.0 33.0 39.0 14-15 38.14747724566114 40.0 38.0 41.0 33.0 41.0 16-17 38.1534318633465 40.0 38.0 41.0 33.0 41.0 18-19 38.15838926350792 40.0 38.0 41.0 33.0 41.0 20-21 38.23972124915286 40.0 38.0 41.0 33.0 41.0 22-23 38.22804725093964 40.0 38.0 41.0 33.0 41.0 24-25 38.22950360604806 40.0 38.0 41.0 33.0 41.0 26-27 38.27534678643784 40.0 38.0 41.0 33.0 41.0 28-29 38.24421543327186 40.0 38.0 41.0 33.0 41.0 30-31 38.20163750776635 40.0 38.0 41.0 33.0 41.0 32-33 38.17130145389421 40.0 38.0 41.0 33.0 41.0 34-35 38.129093078151826 40.0 38.0 41.0 33.0 41.0 36-37 38.060716625149354 40.0 38.0 41.0 33.0 41.0 38-39 38.0122383461867 40.0 38.0 41.0 32.0 41.0 40-41 37.96722876111933 40.0 38.0 41.0 32.0 41.0 42-43 37.823963802096074 40.0 38.0 41.0 32.0 41.0 44-45 37.71139870921645 40.0 37.5 41.0 32.0 41.0 46-47 37.614075504837054 40.0 37.0 41.0 31.0 41.0 48-49 37.519499660312846 40.0 37.0 41.0 31.0 41.0 50-51 37.402519878680394 40.0 37.0 41.0 31.0 41.0 52-53 37.28529324665435 40.0 36.0 41.0 31.0 41.0 54-55 37.17766889447246 40.0 36.0 41.0 31.0 41.0 56-57 37.00384522542535 39.0 35.5 41.0 30.5 41.0 58-59 36.8235229138826 39.0 35.0 41.0 30.0 41.0 60-61 36.61314402652464 39.0 35.0 41.0 30.0 41.0 62-63 36.39032356134816 38.5 35.0 40.5 30.0 41.0 64-65 36.10516441992024 38.0 35.0 40.0 30.0 41.0 66-67 35.86558411950794 37.0 35.0 40.0 29.0 41.0 68-69 35.60100798741789 37.0 35.0 40.0 29.0 41.0 70-71 35.304003483417816 36.5 34.0 39.0 29.0 41.0 72-73 34.948579286466185 36.0 34.0 39.0 29.0 41.0 74-75 34.60432135428029 35.5 34.0 38.5 29.0 40.0 76-77 34.3160706034984 35.0 34.0 37.0 29.0 39.5 78-79 33.98937651576925 35.0 34.0 37.0 28.0 39.0 80-81 33.73378432782728 35.0 34.0 36.5 28.0 39.0 82-83 33.462005016918 35.0 34.0 36.0 28.0 37.5 84-85 33.25921095526333 35.0 34.0 36.0 28.0 37.0 86-87 33.060538140770674 35.0 33.0 35.0 27.5 37.0 88-89 32.890758642943595 35.0 33.0 35.0 27.0 36.0 90-91 32.764548343275095 35.0 33.0 35.0 27.0 36.0 92-93 32.63762410609189 35.0 33.0 35.0 27.0 36.0 94-95 32.53814187669342 35.0 33.0 35.0 27.0 36.0 96-97 32.47169080145984 35.0 33.0 35.0 26.0 35.0 98-99 32.39171148131527 35.0 33.0 35.0 26.0 35.0 100 31.272730628500042 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23989758453066656 1101 2 0.2896171286860678 1101 3 0.26888929078480217 1101 4 0.25507842082788557 1101 5 0.16261422546590865 1101 6 0.3036282575415896 1101 7 0.33407775602007206 1101 8 0.3167793829483543 1101 9 0.461267169040525 1101 10-11 0.4378786868993174 1101 12-13 0.39938327006986896 1101 14-15 0.5136022216388199 1101 16-17 0.506767728629768 1101 18-19 0.4923446883205287 1101 20-21 0.4893448781970804 1101 22-23 0.536590884947465 1101 24-25 0.5451938412608754 1101 26-27 0.5428359628322923 1101 28-29 0.5412999311177913 1101 30-31 0.6083271637964174 1101 32-33 0.6140247461465975 1101 34-35 0.5363786007338263 1101 36-37 0.5861023857212686 1101 38-39 0.5412022699097747 1101 40-41 0.5005916234978187 1101 42-43 0.5563760706566399 1101 44-45 0.48615929211223374 1101 46-47 0.4695597914628351 1101 48-49 0.47195103379709025 1101 50-51 0.42920625487885644 1101 52-53 0.39844223680014323 1101 54-55 0.40718277088943466 1101 56-57 0.43233286616494127 1101 58-59 0.4588570303168922 1101 60-61 0.4662110912187387 1101 62-63 0.4338635088247784 1101 64-65 0.5122788826012794 1101 66-67 0.38877641904735327 1101 68-69 0.3766638161687226 1101 70-71 0.3765347495196565 1101 72-73 0.40715237290451967 1101 74-75 0.3790375405580022 1101 76-77 0.4196456117301537 1101 78-79 0.36213249241738765 1101 80-81 0.36457501388714775 1101 82-83 0.4250542794721355 1101 84-85 0.3856041234925911 1101 86-87 0.36602902014974603 1101 88-89 0.3162393567356787 1101 90-91 0.36670433055718377 1101 92-93 0.3724744960832922 1101 94-95 0.3947707285119506 1101 96-97 0.3897776509648452 1101 98-99 0.3717253537577676 1101 100 0.36836448323256477 1102 1 0.1597805716893106 1102 2 0.22216342270007416 1102 3 0.1379568474421191 1102 4 0.17524410091338893 1102 5 0.16400091959469165 1102 6 0.23600763090770727 1102 7 0.2524110785188043 1102 8 0.2850705091713337 1102 9 0.40646877938800685 1102 10-11 0.36723699968752754 1102 12-13 0.34230793154711847 1102 14-15 0.45866019445515605 1102 16-17 0.4288066105092696 1102 18-19 0.39018082113547337 1102 20-21 0.4087575531691954 1102 22-23 0.4140287502881179 1102 24-25 0.45690961216746473 1102 26-27 0.3908980852124202 1102 28-29 0.4201993622287503 1102 30-31 0.4332762501773004 1102 32-33 0.43854217505487725 1102 34-35 0.3934735453789244 1102 36-37 0.3847064957206925 1102 38-39 0.36964281934347554 1102 40-41 0.3473303213334802 1102 42-43 0.3976799947161638 1102 44-45 0.3375110378652053 1102 46-47 0.37979007017765554 1102 48-49 0.36882386856880345 1102 50-51 0.3504522918960049 1102 52-53 0.2992193105392076 1102 54-55 0.2816582292429288 1102 56-57 0.33997438507918787 1102 58-59 0.28583914021555046 1102 60-61 0.3132718590022563 1102 62-63 0.31826136702181884 1102 64-65 0.34362783528312235 1102 66-67 0.3016800239193884 1102 68-69 0.26725331308365696 1102 70-71 0.29447966268119075 1102 72-73 0.3172002465137922 1102 74-75 0.3171170339715559 1102 76-77 0.26051979694521776 1102 78-79 0.23721429369229696 1102 80-81 0.2297844689407853 1102 82-83 0.21779738681183858 1102 84-85 0.21924439218359737 1102 86-87 0.18792482428607826 1102 88-89 0.19502658195878553 1102 90-91 0.1983524989399683 1102 92-93 0.16300093333440202 1102 94-95 0.190391936061296 1102 96-97 0.1504592181039186 1102 98-99 0.23726586245335568 1102 100 0.19061060629454474 1103 1 0.06973998546796167 1103 2 0.11834221914867982 1103 3 0.10971006775555381 1103 4 0.09291029813673646 1103 5 0.11285498445930386 1103 6 0.15891018578818716 1103 7 0.14269026985786581 1103 8 0.17273075770540203 1103 9 0.2390312271372892 1103 10-11 0.1923486698584398 1103 12-13 0.19522502431944133 1103 14-15 0.23410918182072749 1103 16-17 0.2608087474092926 1103 18-19 0.3098074503652555 1103 20-21 0.23804301623838597 1103 22-23 0.23089443825848122 1103 24-25 0.2819687787170224 1103 26-27 0.2743931764162397 1103 28-29 0.30812555359150906 1103 30-31 0.2549873242822116 1103 32-33 0.2616995829876032 1103 34-35 0.302638228727659 1103 36-37 0.24188373707157496 1103 38-39 0.2969199295896843 1103 40-41 0.20656009863508729 1103 42-43 0.24822309660124375 1103 44-45 0.19146587406570603 1103 46-47 0.22014407641102451 1103 48-49 0.1838610237456777 1103 50-51 0.20798967173463723 1103 52-53 0.1984260300838656 1103 54-55 0.1402721625122325 1103 56-57 0.22664432521798972 1103 58-59 0.2499492242238759 1103 60-61 0.18989562503806212 1103 62-63 0.1522676195734931 1103 64-65 0.11389324259067024 1103 66-67 0.167609821798834 1103 68-69 0.19124192104653304 1103 70-71 0.13603610985402526 1103 72-73 0.15978191317019963 1103 74-75 0.18026016724184046 1103 76-77 0.14099530029925234 1103 78-79 0.11905593969331107 1103 80-81 0.13050915168928867 1103 82-83 0.13301140938103373 1103 84-85 0.12144021428419194 1103 86-87 0.1287309928656839 1103 88-89 0.13038191571974522 1103 90-91 0.2004389343688473 1103 92-93 0.1361409341959572 1103 94-95 0.12954896931890403 1103 96-97 0.193208599188182 1103 98-99 0.1511566989259734 1103 100 0.1533425611291861 1104 1 0.02921963514912207 1104 2 0.08895995838472714 1104 3 0.0903966949803987 1104 4 0.1174835368724132 1104 5 0.11856611612386558 1104 6 0.18923541784251796 1104 7 0.151581490631564 1104 8 0.179791127329743 1104 9 0.24815651117800286 1104 10-11 0.2317644751382133 1104 12-13 0.24371887017674254 1104 14-15 0.33496924582922816 1104 16-17 0.3075661135584511 1104 18-19 0.24712549223461622 1104 20-21 0.2434882609490785 1104 22-23 0.2404122412162124 1104 24-25 0.28304399053131846 1104 26-27 0.239979373849593 1104 28-29 0.23669472267113179 1104 30-31 0.3005635988309123 1104 32-33 0.33306374885744106 1104 34-35 0.25950663895745407 1104 36-37 0.3051733428448813 1104 38-39 0.28832427545003014 1104 40-41 0.30328344989245437 1104 42-43 0.33208863900370034 1104 44-45 0.3131246304691331 1104 46-47 0.2601946080875237 1104 48-49 0.31683634445141706 1104 50-51 0.3026107767498516 1104 52-53 0.30654061431618373 1104 54-55 0.22612130273141418 1104 56-57 0.28822618699787483 1104 58-59 0.3021880723112531 1104 60-61 0.3232888764120361 1104 62-63 0.29642145967422096 1104 64-65 0.3397890592912134 1104 66-67 0.27211052462129004 1104 68-69 0.25537602945718874 1104 70-71 0.21752892476045815 1104 72-73 0.2666496696537024 1104 74-75 0.2247876961569517 1104 76-77 0.1532696581582087 1104 78-79 0.23386509186831717 1104 80-81 0.22528067358476278 1104 82-83 0.19922040911967542 1104 84-85 0.1390357004582441 1104 86-87 0.10903081015614902 1104 88-89 0.11994492638874732 1104 90-91 0.17340467723944641 1104 92-93 0.1725899966042661 1104 94-95 0.1831666496876565 1104 96-97 0.1836044133480499 1104 98-99 0.16958198597734508 1104 100 0.20206879320847904 1105 1 0.0792415982571768 1105 2 0.11217280299164045 1105 3 0.05277745835142511 1105 4 0.1201345427467686 1105 5 0.07214832236630286 1105 6 0.09623379352655803 1105 7 0.12305237214048503 1105 8 0.0980778671180147 1105 9 0.16546953608192183 1105 10-11 0.15595551162409294 1105 12-13 0.14666614143643386 1105 14-15 0.22963457533844434 1105 16-17 0.1853455584773016 1105 18-19 0.2195248348914447 1105 20-21 0.23749968315858894 1105 22-23 0.17884334748710273 1105 24-25 0.231823887097363 1105 26-27 0.18780744220047296 1105 28-29 0.17634545439123883 1105 30-31 0.24796361927782584 1105 32-33 0.24601310699458168 1105 34-35 0.22356210987874903 1105 36-37 0.23397711581250746 1105 38-39 0.21215610737915114 1105 40-41 0.19324701100718045 1105 42-43 0.20437472346909402 1105 44-45 0.17358910758257196 1105 46-47 0.19776684643254328 1105 48-49 0.17904473530499132 1105 50-51 0.18139346710761117 1105 52-53 0.22515974700766606 1105 54-55 0.18448253382733526 1105 56-57 0.1594464816257215 1105 58-59 0.08611171775332593 1105 60-61 0.1792338047144213 1105 62-63 0.12240486826306096 1105 64-65 0.14875177030913989 1105 66-67 0.11583400747502282 1105 68-69 0.13160725377675675 1105 70-71 0.1661366113625462 1105 72-73 0.12762863346834052 1105 74-75 0.16323911677885405 1105 76-77 0.08544142377051855 1105 78-79 0.09668391469797655 1105 80-81 0.07363043904616973 1105 82-83 0.11294871787752925 1105 84-85 0.0801736806971931 1105 86-87 0.04541474363026765 1105 88-89 0.022506457198510077 1105 90-91 0.026020872472869883 1105 92-93 0.14791292705471903 1105 94-95 0.07399173473653065 1105 96-97 0.1095909646381017 1105 98-99 0.1268776352655081 1105 100 0.22450156480138617 1106 1 -0.00860179319988319 1106 2 0.029827581688287808 1106 3 0.06136246492863151 1106 4 0.08295856209549157 1106 5 0.050274649564379104 1106 6 0.07641954448935451 1106 7 0.11988349022247746 1106 8 0.14488597594986885 1106 9 0.19927414679223432 1106 10-11 0.22027307718555988 1106 12-13 0.23707401790691307 1106 14-15 0.2265628310185832 1106 16-17 0.2419682986612557 1106 18-19 0.24152629394194491 1106 20-21 0.23466569695151662 1106 22-23 0.23677283760199686 1106 24-25 0.26606621479748327 1106 26-27 0.21296997040723653 1106 28-29 0.16854519581687555 1106 30-31 0.1685349814897137 1106 32-33 0.1872677281925803 1106 34-35 0.21263440599388161 1106 36-37 0.22703856485292562 1106 38-39 0.201546735598491 1106 40-41 0.17902205955137873 1106 42-43 0.1954271523242923 1106 44-45 0.18626172443755706 1106 46-47 0.22011938312959245 1106 48-49 0.221658080842964 1106 50-51 0.22728690800855134 1106 52-53 0.1899747330379924 1106 54-55 0.1360504465876602 1106 56-57 0.20675358635445207 1106 58-59 0.1871446834969106 1106 60-61 0.18507743321223558 1106 62-63 0.15859205861339376 1106 64-65 0.23834929002445193 1106 66-67 0.23511611316872916 1106 68-69 0.19177105120092364 1106 70-71 0.15220845288438767 1106 72-73 0.18781067753090497 1106 74-75 0.16608240564455912 1106 76-77 0.11684077529933745 1106 78-79 0.21117954271052497 1106 80-81 0.15962712170798454 1106 82-83 0.16005244248454886 1106 84-85 0.17242486771046117 1106 86-87 0.17112564402030017 1106 88-89 0.13789086388437255 1106 90-91 0.17832245460448348 1106 92-93 0.12220742800074902 1106 94-95 0.09121423916544558 1106 96-97 0.18549373845547024 1106 98-99 0.15068762680279946 1106 100 0.22969492228558153 1107 1 0.01028141393825166 1107 2 0.11287469763130531 1107 3 0.09551984726603635 1107 4 0.06177432122865412 1107 5 0.06521987156140341 1107 6 0.13173284307670485 1107 7 0.07887148892812235 1107 8 0.11200130719102219 1107 9 0.20468473008666166 1107 10-11 0.17307624381958675 1107 12-13 0.24915088127882967 1107 14-15 0.2168000359852158 1107 16-17 0.18107651303186145 1107 18-19 0.21235057738398666 1107 20-21 0.22912100562653848 1107 22-23 0.2976950314476241 1107 24-25 0.2985712367784856 1107 26-27 0.21274381838450296 1107 28-29 0.194113193147615 1107 30-31 0.2528847170492412 1107 32-33 0.23539940166283202 1107 34-35 0.24518469009595378 1107 36-37 0.2055300357557428 1107 38-39 0.15707091558386566 1107 40-41 0.20443917433507153 1107 42-43 0.2217013885161947 1107 44-45 0.14801376524382448 1107 46-47 0.17769101883886407 1107 48-49 0.20784990059318176 1107 50-51 0.1842846905038087 1107 52-53 0.16104509705126446 1107 54-55 0.11809852025513123 1107 56-57 0.18472551858853592 1107 58-59 0.15204079201787835 1107 60-61 0.15159581103861086 1107 62-63 0.19451980439228578 1107 64-65 0.22055998135240884 1107 66-67 0.16799031723707714 1107 68-69 0.16261134708189928 1107 70-71 0.11118527592081762 1107 72-73 0.15456797328043592 1107 74-75 0.18628443866133182 1107 76-77 0.19043497544681998 1107 78-79 0.16120214381656695 1107 80-81 0.15745938547647853 1107 82-83 0.15881263039331373 1107 84-85 0.18689760161930735 1107 86-87 0.15846465921010378 1107 88-89 0.12411306982544801 1107 90-91 0.15832140743062695 1107 92-93 0.15690788530983468 1107 94-95 0.1631471630895689 1107 96-97 0.10867493265074302 1107 98-99 0.10875574943582933 1107 100 0.2513951645478336 1108 1 -0.0011149242830121864 1108 2 0.046083842062554226 1108 3 0.04096452504265358 1108 4 0.06309254193241998 1108 5 0.06974421114563967 1108 6 0.03422152626099262 1108 7 0.023778867963152095 1108 8 0.12843680254762546 1108 9 0.09100859897567659 1108 10-11 0.12202911650339843 1108 12-13 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-0.19685003947974877 2214 86-87 -0.18592198350282985 2214 88-89 -0.2672777252440781 2214 90-91 -0.23794946352788315 2214 92-93 -0.19104918919666858 2214 94-95 -0.17093847184570876 2214 96-97 -0.1644668437741572 2214 98-99 -0.21954782356443303 2214 100 -0.17787360981033729 2215 1 -0.33588506839271304 2215 2 -0.41121586049929704 2215 3 -0.46292863735375533 2215 4 -0.41876353009278944 2215 5 -0.36629241980796934 2215 6 -0.4508074256231538 2215 7 -0.4316886735654535 2215 8 -0.42353215246082243 2215 9 -0.620717991543323 2215 10-11 -0.5837741678764132 2215 12-13 -0.5785053675047394 2215 14-15 -0.6597730961394745 2215 16-17 -0.7463846490967612 2215 18-19 -0.7684145490385674 2215 20-21 -0.6743125512230108 2215 22-23 -0.6144385000490189 2215 24-25 -0.5741428349853592 2215 26-27 -0.6207181980854486 2215 28-29 -0.6245224397926421 2215 30-31 -0.6473622161862096 2215 32-33 -0.6412622170010565 2215 34-35 -0.6311174448597257 2215 36-37 -0.6295865519002106 2215 38-39 -0.6837021579002638 2215 40-41 -0.6864132076902862 2215 42-43 -0.6826764714957747 2215 44-45 -0.6310450812449488 2215 46-47 -0.5966465784990973 2215 48-49 -0.6062257059408225 2215 50-51 -0.5674539586516971 2215 52-53 -0.5483649766184655 2215 54-55 -0.6058448934636402 2215 56-57 -0.6120933496354155 2215 58-59 -0.5145988656659952 2215 60-61 -0.4990979253680692 2215 62-63 -0.5138306853073402 2215 64-65 -0.49427073871378724 2215 66-67 -0.4996202439421822 2215 68-69 -0.5927613210548586 2215 70-71 -0.46902084386377396 2215 72-73 -0.37780469427451635 2215 74-75 -0.33768110032852405 2215 76-77 -0.374125123546726 2215 78-79 -0.3568706335585148 2215 80-81 -0.38362166521513785 2215 82-83 -0.399615269357426 2215 84-85 -0.34381737100267884 2215 86-87 -0.3646097149468446 2215 88-89 -0.39112622318210555 2215 90-91 -0.4232846669952295 2215 92-93 -0.463477014062434 2215 94-95 -0.3870858081744757 2215 96-97 -0.42314609227459243 2215 98-99 -0.4011973845245933 2215 100 -0.35912111865153307 2216 1 -0.1359920864143085 2216 2 -0.21449449085111638 2216 3 -0.22772946673180883 2216 4 -0.22152352457396773 2216 5 -0.22186073649175597 2216 6 -0.2721588917654074 2216 7 -0.26769378920684517 2216 8 -0.31002034676732393 2216 9 -0.36748316030127626 2216 10-11 -0.48060272029977824 2216 12-13 -0.4316034469256067 2216 14-15 -0.5685266616818794 2216 16-17 -0.548650414473947 2216 18-19 -0.5304531576496672 2216 20-21 -0.5278131574740001 2216 22-23 -0.5138934043284493 2216 24-25 -0.5122117787889167 2216 26-27 -0.5803486245091491 2216 28-29 -0.6021745755864458 2216 30-31 -0.6109288911637805 2216 32-33 -0.6153192497955544 2216 34-35 -0.5820206726530301 2216 36-37 -0.5441240670121275 2216 38-39 -0.4954044072599757 2216 40-41 -0.535376309812932 2216 42-43 -0.48438008915523767 2216 44-45 -0.43398473107342284 2216 46-47 -0.4308653955226518 2216 48-49 -0.3906247148169868 2216 50-51 -0.41556173614512204 2216 52-53 -0.4096272198172457 2216 54-55 -0.4252445364299291 2216 56-57 -0.4016022440906255 2216 58-59 -0.38453018960814944 2216 60-61 -0.392940846924148 2216 62-63 -0.38710808085208015 2216 64-65 -0.3539328442968497 2216 66-67 -0.3388670978032948 2216 68-69 -0.35890205129517483 2216 70-71 -0.3477474645892542 2216 72-73 -0.28724039250911915 2216 74-75 -0.2729505637057912 2216 76-77 -0.32859767462603884 2216 78-79 -0.2825547538550026 2216 80-81 -0.3114725170799417 2216 82-83 -0.2754855274215515 2216 84-85 -0.2787170470464133 2216 86-87 -0.30258121122295023 2216 88-89 -0.3045119099980198 2216 90-91 -0.36116188408225725 2216 92-93 -0.262759968393496 2216 94-95 -0.317639273800161 2216 96-97 -0.3292373347591919 2216 98-99 -0.308516020175567 2216 100 -0.33489900275405304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 2123.0 1 570.0 2 421.0 3 356.0 4 286.0 5 367.0 6 483.0 7 901.0 8 2534.0 9 3814.0 10 4488.0 11 6079.0 12 8790.0 13 11599.0 14 14396.0 15 15975.0 16 17435.0 17 19488.0 18 21712.0 19 24024.0 20 26732.0 21 29648.0 22 33300.0 23 38525.0 24 45706.0 25 55955.0 26 61419.0 27 62208.0 28 69144.0 29 84452.0 30 98643.0 31 116603.0 32 148258.0 33 197235.0 34 287102.0 35 437654.0 36 807777.0 37 1699975.0 38 2163883.0 39 612343.0 40 723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.19041561700995 19.858273509127656 13.31577770360296 24.635533170259443 2 14.952894020607605 20.300614476342147 40.73420086949135 24.01229063355889 3 18.213744376486083 25.72596339103683 30.05752362688716 26.002768605589928 4 12.300060797069785 17.325421504673518 39.847553492151036 30.526964206105667 5 13.230724996309092 38.14664360017672 35.623115545210105 12.99951585830408 6 30.18427233343956 38.16392705822802 18.645329137008464 13.006471471323952 7 26.518854014226857 32.428680307562196 23.430128232093004 17.622337446117946 8 24.97426246748215 35.879019759783034 21.02065921292965 18.12605855980517 9 25.918543932744107 15.757869824253529 20.990708373421615 37.33287786958075 10-11 24.410757988835723 26.406195865253807 29.107530077472532 20.075516068437942 12-13 24.993265810196704 24.07998239080271 28.46332775367489 22.4634240453257 14-15 22.4352875340535 25.406986884035348 25.931939552095645 26.225786029815506 16-17 22.371476257599213 28.06366128488287 26.80613298511722 22.758729472400702 18-19 22.225619816986484 27.985778990457753 27.808396871045826 21.980204321509934 20-21 23.366895317048332 27.24774208901039 27.19213826384354 22.19322433009773 22-23 23.20615027865251 27.252014488823463 26.850396533308245 22.691438699215787 24-25 22.887870710570247 27.32743872193793 27.17699261363361 22.607697953858214 26-27 22.74659015420668 27.5377340080586 26.80397615942435 22.911699678310367 28-29 22.616310467309038 27.687227257085762 27.00977256566896 22.686689709936235 30-31 23.0206761101582 27.39903539344468 26.88429121509542 22.6959972813017 32-33 22.854990776117866 27.568061069682514 26.64371489154674 22.93323326265288 34-35 23.08802289833123 27.603109429544702 26.859944559968536 22.448923112155537 36-37 23.08740979402217 27.301234466596863 26.802405432779402 22.80895030660156 38-39 22.960556221688854 27.39088771160495 27.035306294855893 22.613249771850306 40-41 23.065872495825843 27.083627805720802 27.130224493640675 22.720275204812683 42-43 22.891466064528718 27.138275278844553 26.94639742972799 23.023861226898738 44-45 22.94696400210745 27.439541397801293 26.911938539390327 22.70155606070093 46-47 22.86220622660026 27.222895048448674 26.798070147582443 23.11682857736862 48-49 22.94550485150588 27.08320965338756 27.04958254645211 22.921702948654453 50-51 22.932491366584323 27.162650562396667 26.831892078291062 23.072965992727948 52-53 22.792572353899175 27.254454662019572 26.88986295705516 23.06311002702609 54-55 22.704129604806795 27.09748990178045 26.99360530272934 23.204775190683417 56-57 22.99255196715927 27.08959621702509 26.87993758329418 23.037914232521466 58-59 22.776983920003495 27.144988444796752 27.114639559239524 22.963388075960232 60-61 22.80132736363821 27.272135977967505 26.97692640781904 22.949610250575244 62-63 22.749396500418595 27.104875831003373 26.91867267526467 23.227054993313363 64-65 22.531894430681703 27.062854574294263 27.037812470137517 23.367438524886516 66-67 22.823788617891804 26.998086339940897 26.83292547105412 23.345199571113177 68-69 22.69273925785034 26.961408970584817 27.018674971574587 23.327176799990255 70-71 22.647568093540034 26.784140670426787 26.972598863742753 23.595692372290426 72-73 22.761393560186903 26.81477137041574 27.11080963486469 23.313025434532662 74-75 22.756138387837193 26.96764367422379 27.037450687725023 23.238767250213996 76-77 22.712008381585505 26.87838874937801 26.946588428017478 23.463014441019006 78-79 22.681796459048957 26.866695814055713 26.971971034747533 23.479536692147796 80-81 22.65014500658171 26.947627889397392 27.038594772704926 23.363632331315976 82-83 22.9571348703803 26.62641116895607 27.30644757135244 23.110006389311184 84-85 22.99273702112477 26.681709443062747 27.002567877772087 23.32298565804039 86-87 22.57044909992979 26.9219434483567 27.195694492948537 23.311912958764978 88-89 22.9375510743518 26.4914195088974 27.056442824057896 23.5145865926929 90-91 22.87367629244183 26.490072915535762 27.121649590397183 23.514601201625222 92-93 23.027004332333888 26.45788148637509 27.120317437868778 23.39479674342224 94-95 22.640344548055367 26.493552662641978 27.276010690809276 23.590092098493383 96-97 22.70332387103488 26.39407691666895 27.296489318451595 23.60610989384458 98-99 22.626531747816095 26.535443047522406 27.1348940080992 23.7031311965623 100 22.96923375645482 26.278057744448187 26.879110251584713 23.87359824751228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1981.0 1 1231.5 2 462.5 3 453.5 4 454.0 5 427.0 6 391.0 7 367.5 8 387.5 9 393.5 10 375.0 11 414.5 12 436.5 13 442.0 14 503.5 15 589.5 16 674.0 17 786.5 18 954.5 19 1187.0 20 1609.0 21 2304.5 22 3533.0 23 5575.0 24 8448.0 25 12233.5 26 18334.5 27 26916.0 28 36816.5 29 49906.0 30 66158.5 31 83033.0 32 102603.5 33 120668.5 34 136400.5 35 154259.0 36 174891.0 37 201103.0 38 218785.5 39 225253.5 40 231935.5 41 235831.5 42 241799.0 43 258579.5 44 277882.5 45 297950.5 46 312971.5 47 320120.5 48 322611.5 49 314920.5 50 303033.5 51 295673.5 52 286242.5 53 272318.5 54 257050.0 55 232488.5 56 207765.0 57 180380.5 58 152438.5 59 134113.0 60 110968.0 61 86285.0 62 67817.0 63 51814.0 64 36738.0 65 24408.0 66 17062.5 67 11891.0 68 8012.0 69 5770.0 70 4513.0 71 3273.0 72 2178.5 73 1543.0 74 1196.0 75 890.0 76 601.5 77 385.5 78 243.5 79 137.5 80 85.5 81 74.0 82 58.5 83 37.0 84 27.5 85 27.5 86 27.5 87 21.5 88 16.5 89 19.0 90 18.5 91 13.5 92 12.5 93 9.5 94 11.0 95 13.5 96 10.0 97 7.5 98 9.5 99 11.0 100 17.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09614100459469392 2 0.06265617383134207 3 0.07826491616487809 4 0.08024193135858548 5 0.0816106341849983 6 0.08965694777057664 7 0.08613150109648304 8 0.08745872807967123 9 0.07366109756694408 10-11 0.08004837742353721 12-13 0.070301554265749 14-15 0.07897691814023425 16-17 0.08037327152879682 18-19 0.08006220270461209 20-21 0.0719744132758091 22-23 0.07584549197677463 24-25 0.07771190492188301 26-27 0.07184307310559777 28-29 0.08091937013125446 30-31 0.08485957523759437 32-33 0.08618680222078255 34-35 0.0884610609575998 36-37 0.08850253680082443 38-39 0.09001640507852345 40-41 0.08595868508304708 42-43 0.0919865076316934 44-45 0.08825368174147664 46-47 0.08500474068888057 48-49 0.09419163996313627 50-51 0.09061089216474315 52-53 0.09623086892168062 54-55 0.0878873117929924 56-57 0.09477921440881855 58-59 0.09899592513665598 60-61 0.09778621304260426 62-63 0.09881619648268258 64-65 0.10086233808176437 66-67 0.10290847968084614 68-69 0.09951437317696388 70-71 0.1021066133785033 72-73 0.09956967430126337 74-75 0.1035651805319028 76-77 0.10072408527101559 78-79 0.10754686148146736 80-81 0.1025766729350491 82-83 0.10752612355985502 84-85 0.10382094823178803 86-87 0.09978396615792397 88-89 0.0987263321556959 90-91 0.09902357569880574 92-93 0.09355567703369193 94-95 0.09307179219607124 96-97 0.0905832416025934 98-99 0.1050375729663772 100 0.24585497335453577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7233126.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.42224545284495 #Duplication Level Percentage of deduplicated Percentage of total 1 68.04093940606806 34.98817886980983 2 15.08447115216999 15.51354756226488 3 6.472642519088548 9.985134370352759 4 3.493526868575815 7.185799845280576 5 2.013798974538029 5.177703258069098 6 1.275914199681278 3.936622389166854 7 0.8348543005624152 3.0051057932618592 8 0.6200399748835469 2.5507078223230053 9 0.4319110682343337 1.9988853268091733 >10 1.6873079376875049 13.556753884996512 >50 0.033932375261919084 1.1585423311536807 >100 0.01048900273841526 0.8789899170802825 >500 1.722205101198035E-4 0.06402862943138404 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.3825281074876894E-5 0.0 3 0.0 0.0 0.0 1.3825281074876894E-5 0.0 4 0.0 0.0 0.0 1.3825281074876894E-5 0.0 5 0.0 0.0 0.0 1.3825281074876894E-5 0.0 6 0.0 0.0 0.0 1.3825281074876894E-5 0.0 7 0.0 0.0 0.0 1.3825281074876894E-5 0.0 8 0.0 0.0 0.0 1.3825281074876894E-5 0.0 9 0.0 0.0 0.0 2.7650562149753787E-5 0.0 10-11 0.0 0.0 0.0 7.603904591182291E-5 0.0 12-13 0.0 0.0 0.0 8.986432698669981E-5 2.073792161231534E-5 14-15 0.0 0.0 0.0 9.677696752413825E-5 4.147584322463068E-5 16-17 0.0 0.0 0.0 9.677696752413825E-5 5.5301124299507574E-5 18-19 6.912640537438447E-6 0.0 0.0 1.1060224859901515E-4 5.5301124299507574E-5 20-21 1.3825281074876894E-5 0.0 0.0 1.3825281074876893E-4 5.5301124299507574E-5 22-23 1.3825281074876894E-5 0.0 0.0 1.6590337289852273E-4 5.5301124299507574E-5 24-25 1.3825281074876894E-5 0.0 0.0 3.4563202687192234E-4 5.5301124299507574E-5 26-27 1.3825281074876894E-5 0.0 0.0 7.189146158935985E-4 5.5301124299507574E-5 28-29 1.3825281074876894E-5 0.0 0.0 0.0023364725016541948 5.5301124299507574E-5 30-31 1.3825281074876894E-5 0.0 0.0 0.008862005168996087 5.5301124299507574E-5 32-33 1.3825281074876894E-5 0.0 0.0 0.027035337141921762 5.5301124299507574E-5 34-35 1.3825281074876894E-5 0.0 0.0 0.055556891999392795 5.5301124299507574E-5 36-37 1.3825281074876894E-5 0.0 0.0 0.10223795354871462 5.5301124299507574E-5 38-39 1.3825281074876894E-5 0.0 0.0 0.17657648988832766 6.221376483694602E-5 40-41 1.3825281074876894E-5 0.0 0.0 0.2606134609019669 6.912640537438447E-5 42-43 1.3825281074876894E-5 0.0 0.0 0.34750535245756814 7.603904591182291E-5 44-45 1.3825281074876894E-5 0.0 0.0 0.456731985589633 8.986432698669981E-5 46-47 1.3825281074876894E-5 0.0 0.0 0.5921644389991271 9.677696752413825E-5 48-49 1.3825281074876894E-5 0.0 0.0 0.743046644009796 9.677696752413825E-5 50-51 1.3825281074876894E-5 0.0 0.0 0.9033991665567557 9.677696752413825E-5 52-53 1.3825281074876894E-5 0.0 0.0 1.0642701371440233 1.2442752967389205E-4 54-55 1.3825281074876894E-5 0.0 0.0 1.2448352206224529 1.2442752967389205E-4 56-57 1.3825281074876894E-5 0.0 0.0 1.4585118522752127 1.2442752967389205E-4 58-59 1.3825281074876894E-5 0.0 0.0 1.7058876065479849 1.2442752967389205E-4 60-61 1.3825281074876894E-5 0.0 0.0 1.9660033573312563 1.2442752967389205E-4 62-63 1.3825281074876894E-5 0.0 0.0 2.2298519340047442 1.659033728985227E-4 64-65 1.3825281074876894E-5 0.0 0.0 2.5105397030274323 1.7972865397339961E-4 66-67 1.3825281074876894E-5 0.0 0.0 2.8294474615816174 1.7972865397339961E-4 68-69 1.3825281074876894E-5 0.0 0.0 3.188697390312294 1.7972865397339961E-4 70-71 1.3825281074876894E-5 0.0 0.0 3.5787016567940335 1.7972865397339961E-4 72-73 1.3825281074876894E-5 0.0 0.0 3.975362796113326 1.8664129451083806E-4 74-75 1.3825281074876894E-5 0.0 0.0 4.378183098151477 1.935539350482765E-4 76-77 1.3825281074876894E-5 1.3825281074876894E-5 0.0 4.818718766961892 2.0737921612315338E-4 78-79 1.3825281074876894E-5 2.073792161231534E-5 0.0 5.307864124031574 2.2811713773546874E-4 80-81 1.3825281074876894E-5 2.7650562149753787E-5 0.0 5.839667385857789 2.3502977827290718E-4 82-83 1.3825281074876894E-5 2.7650562149753787E-5 0.0 6.387438847325486 2.3502977827290718E-4 84-85 1.3825281074876894E-5 2.7650562149753787E-5 0.0 6.937104372300441 2.3502977827290718E-4 86-87 1.3825281074876894E-5 2.7650562149753787E-5 0.0 7.504991617732085 2.488550593477841E-4 88 1.3825281074876894E-5 2.7650562149753787E-5 0.0 7.956214228813379 2.62680340422661E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5890 0.0 35.287514 1 GTATCAA 9895 0.0 34.45373 1 CAACGCA 9135 0.0 34.219517 5 ATCAACG 9355 0.0 33.265877 3 AACGCAG 10580 0.0 30.246065 6 TATCAAC 11290 0.0 28.980114 2 TCAACGC 10855 0.0 28.797949 4 ACGCAGA 11520 0.0 27.003246 7 CGCAGAG 11930 0.0 25.917307 8 GTACATG 20960 0.0 23.78092 1 TACATGG 20955 0.0 22.85974 2 ACATGGG 20560 0.0 22.27002 3 GTACTAG 2995 0.0 20.096813 1 CATGGGG 15885 0.0 19.945385 4 GCAGAGT 15800 0.0 19.09257 9 GTATAAG 3845 0.0 17.610846 1 ATGGGGG 10535 0.0 17.490904 5 GTCGCCG 1545 0.0 17.368887 94 GAGTACA 16120 0.0 17.298313 1 CTAGTAC 3950 0.0 17.256618 3 >>END_MODULE