FastQCFastQC Report
Mon 16 Jan 2017
ERR1146630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233126
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104150.14399030239484284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95210.1316305011139029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102400.050.609681
GTATCAA182600.041.6190871
ATCAACG212400.034.649723
CAACGCA216000.034.2068825
TATCAAC230650.032.561652
AACGCAG232650.032.243456
TCAACGC229650.032.2311064
ACGCAGA253000.029.1834967
CGCAGAG256300.028.7527338
GTGGTAT41400.024.6496811
GCAGAGT299750.024.2241179
GTACATG203200.023.2591741
TACATGG204850.022.1077882
ACATGGG201850.021.736663
CAGAGTA285300.021.143310-11
GTACTAG30750.020.9520911
GAGTACT193100.020.9432612-13
AGAGTAC276300.020.83693710-11
TGGTATC44050.020.6047022
CATGGGG157850.019.2453084
Produced by FastQC (version 0.11.5)