FastQCFastQC Report
Mon 16 Jan 2017
ERR1146629_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146629_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161389
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT684791.6455803579045363No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA118850.2856017546064547No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA109530.2632053864707193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13950.023.9331281
CGTTAAC2807.6397555E-1121.8323571
CATGGGG76350.021.3035374
CTAACGC4750.019.7939073
CTGTGCG21650.019.5347279
CTAGTAC24150.018.8820763
TACCTGG34200.018.8317032
GGCGTCG7750.018.7974248
TAGTACT25000.018.615724
ATGGGGG51850.017.9506515
GAGTACG11550.017.913731
CGACGAG7100.017.86993424-25
TACGGGC8700.017.831154
TGCGCGA8200.017.79775294
CGATAAT10300.017.79731866-67
CTCGTCG12450.017.3624579
TTTGCGT9800.017.2691733
TAACGCC5450.017.2511564
ACGGGCG9000.017.2359495
TCTAACG5550.016.940732
Produced by FastQC (version 0.11.5)