##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146629_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4161389
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280420311583462	31.0	31.0	34.0	28.0	34.0
2	31.335506726239725	31.0	31.0	34.0	28.0	34.0
3	31.49023871596719	33.0	31.0	34.0	28.0	34.0
4	34.991683786351146	37.0	35.0	37.0	32.0	37.0
5	34.99667659043651	37.0	35.0	37.0	32.0	37.0
6	35.16933000015139	37.0	35.0	37.0	32.0	37.0
7	35.07323251923817	37.0	35.0	37.0	32.0	37.0
8	35.07088618728026	37.0	35.0	37.0	32.0	37.0
9	36.7055370214128	39.0	37.0	39.0	32.0	39.0
10-11	36.70442501289834	39.0	37.0	39.0	32.0	39.0
12-13	36.683197365110544	39.0	37.0	39.0	32.0	39.0
14-15	37.973635966260304	40.0	38.0	41.0	32.5	41.0
16-17	37.99450267687063	40.0	38.0	41.0	33.0	41.0
18-19	38.015562472049595	40.0	38.0	41.0	32.0	41.0
20-21	38.11223127662423	40.0	38.0	41.0	33.0	41.0
22-23	38.08409848250187	40.0	38.0	41.0	32.0	41.0
24-25	38.07552009677538	40.0	38.0	41.0	32.0	41.0
26-27	38.09718822249013	40.0	38.0	41.0	32.0	41.0
28-29	38.074637578943	40.0	38.0	41.0	32.5	41.0
30-31	38.057190760104376	40.0	38.0	41.0	32.0	41.0
32-33	37.99938085576714	40.0	38.0	41.0	32.0	41.0
34-35	37.895986412229185	40.0	38.0	41.0	32.0	41.0
36-37	37.817571849207084	40.0	38.0	41.0	32.0	41.0
38-39	37.75214681924713	40.0	38.0	41.0	31.0	41.0
40-41	37.73874948965357	40.0	38.0	41.0	31.0	41.0
42-43	37.56670056560442	40.0	37.5	41.0	31.0	41.0
44-45	37.402717097584485	40.0	37.0	41.0	30.5	41.0
46-47	37.260234623583614	40.0	37.0	41.0	30.0	41.0
48-49	37.13636913059558	40.0	36.0	41.0	30.0	41.0
50-51	36.97914434339111	40.0	36.0	41.0	30.0	41.0
52-53	36.90411891318019	39.5	36.0	41.0	30.0	41.0
54-55	36.77611537878339	39.0	35.5	41.0	30.0	41.0
56-57	36.48330124869365	39.0	35.0	41.0	29.0	41.0
58-59	36.30447477993526	39.0	35.0	40.5	29.0	41.0
60-61	36.044643266947645	38.5	35.0	40.0	28.0	41.0
62-63	35.84743988125119	38.0	35.0	40.0	28.0	41.0
64-65	35.579695745819485	37.5	34.0	40.0	28.0	41.0
66-67	35.257861257383055	37.0	34.0	40.0	28.0	41.0
68-69	34.930860465099514	36.5	34.0	39.0	28.0	41.0
70-71	34.56257045424016	36.0	34.0	39.0	27.5	41.0
72-73	34.219372065432964	35.0	33.0	39.0	26.5	40.0
74-75	33.916507444990124	35.0	33.0	37.5	26.0	40.0
76-77	33.630063423534786	35.0	33.0	37.0	26.0	39.0
78-79	33.26565817326859	35.0	33.0	36.5	26.0	39.0
80-81	32.92031374620349	35.0	32.5	36.0	26.0	38.5
82-83	32.67499841999871	35.0	32.0	36.0	25.5	37.0
84-85	32.49628597566822	35.0	32.0	35.0	25.0	37.0
86-87	32.38029633855427	35.0	32.0	35.0	25.0	36.5
88-89	32.17143855092615	35.0	32.0	35.0	25.0	36.0
90-91	31.946369108968184	35.0	32.0	35.0	25.0	36.0
92-93	31.741738275369112	35.0	32.0	35.0	24.0	36.0
94-95	31.638763042820557	35.0	32.0	35.0	24.0	35.0
96-97	31.584344313881736	35.0	32.0	35.0	24.0	35.0
98-99	31.528998971256954	35.0	32.0	35.0	24.0	35.0
100	30.24906034980147	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.315924986079235
1101	2	0.3283640892895612
1101	3	0.33025344376071075
1101	4	0.40964357713334465
1101	5	0.24395596544985665
1101	6	0.3329298908445111
1101	7	0.34349022407565144
1101	8	0.39744357864020685
1101	9	0.4815067319809643
1101	10-11	0.4444479894860578
1101	12-13	0.47640971822192313
1101	14-15	0.6640310614289362
1101	16-17	0.6025092570584079
1101	18-19	0.5571340273290843
1101	20-21	0.5494528053343686
1101	22-23	0.5558746390384002
1101	24-25	0.6082252750577837
1101	26-27	0.5401465125137506
1101	28-29	0.5468732130862648
1101	30-31	0.6078067001784007
1101	32-33	0.6284594728616568
1101	34-35	0.6170827854584644
1101	36-37	0.6033106228600715
1101	38-39	0.6241414458312065
1101	40-41	0.5804096028496986
1101	42-43	0.6082573659283526
1101	44-45	0.5540722298336291
1101	46-47	0.5401514257203743
1101	48-49	0.6202845606959428
1101	50-51	0.5600993760148185
1101	52-53	0.4913802482384213
1101	54-55	0.6018595477367725
1101	56-57	0.6039156652084898
1101	58-59	0.55970332054153
1101	60-61	0.6114099751856514
1101	62-63	0.547097491232968
1101	64-65	0.5530781069183917
1101	66-67	0.5457896799902997
1101	68-69	0.600725932402213
1101	70-71	0.5363599310513365
1101	72-73	0.5322286285798512
1101	74-75	0.489279163664726
1101	76-77	0.5065979376939964
1101	78-79	0.5155580876877721
1101	80-81	0.4919571961438365
1101	82-83	0.49012343078972975
1101	84-85	0.42477196086706925
1101	86-87	0.44802869115031996
1101	88-89	0.4239750365232311
1101	90-91	0.46149318218373736
1101	92-93	0.5259978426056797
1101	94-95	0.48833044587048136
1101	96-97	0.448725950850676
1101	98-99	0.47307601337021765
1101	100	0.502376203878633
1102	1	0.26964277077470555
1102	2	0.2881320495176922
1102	3	0.2496355701094437
1102	4	0.25609185857361183
1102	5	0.19723267901382968
1102	6	0.24837210550591493
1102	7	0.2502360004799513
1102	8	0.2958812903860135
1102	9	0.31594476660580284
1102	10-11	0.36601716188869915
1102	12-13	0.3885732013254142
1102	14-15	0.44084892123315456
1102	16-17	0.36055919896315203
1102	18-19	0.3673726395267636
1102	20-21	0.4035758950898156
1102	22-23	0.3984287828002877
1102	24-25	0.4529204939871576
1102	26-27	0.5153571631904654
1102	28-29	0.4910207845535908
1102	30-31	0.5184160326292755
1102	32-33	0.3812490951883518
1102	34-35	0.42323610837260617
1102	36-37	0.4416824167516893
1102	38-39	0.4587077625263163
1102	40-41	0.3793510911973428
1102	42-43	0.42407046490751554
1102	44-45	0.34535356083024027
1102	46-47	0.3926976582897481
1102	48-49	0.33756329750391956
1102	50-51	0.33771619735607317
1102	52-53	0.29120673357219573
1102	54-55	0.2541796665457525
1102	56-57	0.42148581346706493
1102	58-59	0.3045786331255158
1102	60-61	0.3102809321839999
1102	62-63	0.3049404657100965
1102	64-65	0.4011994694022789
1102	66-67	0.32892976716107114
1102	68-69	0.3423894845768771
1102	70-71	0.33566764428356777
1102	72-73	0.3583105768529222
1102	74-75	0.29998723016073825
1102	76-77	0.36130869025479484
1102	78-79	0.2601985582773949
1102	80-81	0.22659125509091638
1102	82-83	0.3513583249566068
1102	84-85	0.3160973103819842
1102	86-87	0.262138225892798
1102	88-89	0.20345333422885403
1102	90-91	0.2015078036162734
1102	92-93	0.2780870405630118
1102	94-95	0.28168556916335064
1102	96-97	0.26508878091163623
1102	98-99	0.3007547690784307
1102	100	0.3191874387563942
1103	1	0.18424045544018952
1103	2	0.24856959927858924
1103	3	0.1967443690729347
1103	4	0.2372650537055705
1103	5	0.2199554636132106
1103	6	0.3001826192939063
1103	7	0.28669683216077146
1103	8	0.26517582711306176
1103	9	0.3599600241426728
1103	10-11	0.36574896814963154
1103	12-13	0.3789488868513544
1103	14-15	0.440082500342065
1103	16-17	0.4505628419143761
1103	18-19	0.415351955476126
1103	20-21	0.4033533618705576
1103	22-23	0.4436892373285133
1103	24-25	0.4650656977369252
1103	26-27	0.39234302491326645
1103	28-29	0.4405420084533347
1103	30-31	0.48410437732829337
1103	32-33	0.44041087024517367
1103	34-35	0.4151280693188468
1103	36-37	0.41925840360409694
1103	38-39	0.47320481272486603
1103	40-41	0.38940019686105387
1103	42-43	0.41374920935860615
1103	44-45	0.3526100394316103
1103	46-47	0.42311300418055
1103	48-49	0.41623068616725334
1103	50-51	0.39008411564847734
1103	52-53	0.3877830187839564
1103	54-55	0.3169140888083106
1103	56-57	0.33561065625146114
1103	58-59	0.35104744303434643
1103	60-61	0.3922996711294644
1103	62-63	0.3585250637738966
1103	64-65	0.3046707403636688
1103	66-67	0.294707537246353
1103	68-69	0.29045146749621154
1103	70-71	0.27794829658612485
1103	72-73	0.30619601675164887
1103	74-75	0.3713434135347029
1103	76-77	0.32728789411336123
1103	78-79	0.34882389640426226
1103	80-81	0.258655654556172
1103	82-83	0.3110049751723025
1103	84-85	0.3251375987850338
1103	86-87	0.4095340927091442
1103	88-89	0.33580463670755023
1103	90-91	0.3127625448101483
1103	92-93	0.2849554871657567
1103	94-95	0.3463128425050215
1103	96-97	0.3927866665163755
1103	98-99	0.3489616458494247
1103	100	0.3304578396953204
1104	1	0.1260890037133322
1104	2	0.13354699983679197
1104	3	0.0607722431364941
1104	4	0.2089788186997481
1104	5	0.099544023569905
1104	6	0.17691589733056645
1104	7	0.19651668921540022
1104	8	0.22123571523975727
1104	9	0.30773212618237267
1104	10-11	0.3099794821407258
1104	12-13	0.3567934893253337
1104	14-15	0.3953864097917119
1104	16-17	0.38454553285734505
1104	18-19	0.3640478965930498
1104	20-21	0.37678698268985045
1104	22-23	0.3187683125184364
1104	24-25	0.4363271113456406
1104	26-27	0.36468355910940176
1104	28-29	0.3702358811467761
1104	30-31	0.38982134095449794
1104	32-33	0.37723090479141064
1104	34-35	0.4308708969082957
1104	36-37	0.3726243285576132
1104	38-39	0.2888300166589417
1104	40-41	0.32158565131365435
1104	42-43	0.3206086451496759
1104	44-45	0.3247141125644575
1104	46-47	0.34979614005053605
1104	48-49	0.35878434585185204
1104	50-51	0.32409529036862494
1104	52-53	0.305249863635801
1104	54-55	0.25256721850864494
1104	56-57	0.36468854164863274
1104	58-59	0.374369635065122
1104	60-61	0.36131127618006076
1104	62-63	0.3388603022258323
1104	64-65	0.3729087594438596
1104	66-67	0.3741269442352291
1104	68-69	0.35320286796172695
1104	70-71	0.3267034751322768
1104	72-73	0.4128556877900351
1104	74-75	0.31980399699973816
1104	76-77	0.3182920174041399
1104	78-79	0.32870301610894614
1104	80-81	0.31603385133604434
1104	82-83	0.25323572939183236
1104	84-85	0.23512783832303796
1104	86-87	0.19678278682174977
1104	88-89	0.2190670837398585
1104	90-91	0.18011758256100308
1104	92-93	0.24100542882681353
1104	94-95	0.1390152735725465
1104	96-97	0.14731131530840713
1104	98-99	0.171252100930964
1104	100	0.1761129366205516
1105	1	0.1392874843433347
1105	2	0.13410211638665004
1105	3	0.10844580014117966
1105	4	0.15214276147966643
1105	5	0.09374668665231667
1105	6	0.15482324333188302
1105	7	0.1888989146542599
1105	8	0.20690115351104055
1105	9	0.2739487914951013
1105	10-11	0.2820802597962384
1105	12-13	0.27372786741016597
1105	14-15	0.3575135524062034
1105	16-17	0.3115095499810323
1105	18-19	0.27399469894940864
1105	20-21	0.3034752878242415
1105	22-23	0.2573165411919902
1105	24-25	0.28827531195565825
1105	26-27	0.2970208828814407
1105	28-29	0.2877475312091775
1105	30-31	0.2969999457041226
1105	32-33	0.30773363606330406
1105	34-35	0.3008056122479772
1105	36-37	0.32844271313152973
1105	38-39	0.3008696453339681
1105	40-41	0.26658049402242767
1105	42-43	0.3605585738830541
1105	44-45	0.2821787685029733
1105	46-47	0.28019978732627493
1105	48-49	0.24988795694039112
1105	50-51	0.3518240390054501
1105	52-53	0.3278287061870273
1105	54-55	0.3071169586747118
1105	56-57	0.31095424430802154
1105	58-59	0.3588134476695899
1105	60-61	0.3702916095453901
1105	62-63	0.375657390510284
1105	64-65	0.4079463375109711
1105	66-67	0.3351820103199472
1105	68-69	0.33704848401800547
1105	70-71	0.35803248077206007
1105	72-73	0.2943333809668971
1105	74-75	0.3699152664811223
1105	76-77	0.3270292564932049
1105	78-79	0.27359077425045086
1105	80-81	0.26366765165655437
1105	82-83	0.2836951344939749
1105	84-85	0.23686150054138722
1105	86-87	0.19853675818216487
1105	88-89	0.22224146418794533
1105	90-91	0.2512549683682366
1105	92-93	0.3246193975778162
1105	94-95	0.2864011792741259
1105	96-97	0.30827849819653963
1105	98-99	0.22928824381870072
1105	100	0.2729038460023929
1106	1	0.11377144530863248
1106	2	0.11818625510318981
1106	3	0.13381962879818232
1106	4	0.1796955335305057
1106	5	0.08925881170926431
1106	6	0.12542619266096722
1106	7	0.24072032937935717
1106	8	0.1595089783341237
1106	9	0.16698063153086196
1106	10-11	0.24968525956600018
1106	12-13	0.23461953142977165
1106	14-15	0.2435659822999625
1106	16-17	0.2555355114405842
1106	18-19	0.21786074214200823
1106	20-21	0.23814098150819518
1106	22-23	0.2935130304328979
1106	24-25	0.30615781741324355
1106	26-27	0.2936453213791239
1106	28-29	0.17183473720837839
1106	30-31	0.18129666248424314
1106	32-33	0.27491371164146017
1106	34-35	0.2408965393275153
1106	36-37	0.25226208359222113
1106	38-39	0.22447398025652632
1106	40-41	0.1933233601210489
1106	42-43	0.23932915226805562
1106	44-45	0.19367009931196577
1106	46-47	0.29127637826024966
1106	48-49	0.2406318647163488
1106	50-51	0.27215340470311844
1106	52-53	0.23080677704448505
1106	54-55	0.19155989909955906
1106	56-57	0.29623077938461506
1106	58-59	0.20135398433773588
1106	60-61	0.21314785220961596
1106	62-63	0.2211910295451176
1106	64-65	0.2605120021821321
1106	66-67	0.29008238787194784
1106	68-69	0.23660607866067807
1106	70-71	0.20344019703887994
1106	72-73	0.27183388542674436
1106	74-75	0.276677652693607
1106	76-77	0.26487928211165723
1106	78-79	0.26839080501188306
1106	80-81	0.21439687660843987
1106	82-83	0.19578591448988902
1106	84-85	0.1251667023061671
1106	86-87	0.1843607523955555
1106	88-89	0.2112769841289719
1106	90-91	0.22223556873057504
1106	92-93	0.21150325131070602
1106	94-95	0.12734202509933823
1106	96-97	0.20152270260244265
1106	98-99	0.22155478249863592
1106	100	0.3391693214792859
1107	1	0.10356933695339521
1107	2	0.12021851882448686
1107	3	0.11111931679999643
1107	4	0.13577720083573297
1107	5	0.1392206638497413
1107	6	0.1439301890608533
1107	7	0.17148067531114464
1107	8	0.21455111965809692
1107	9	0.2735630198038379
1107	10-11	0.24304321819987962
1107	12-13	0.23147009315668043
1107	14-15	0.24509835463226182
1107	16-17	0.3076630108018108
1107	18-19	0.25959327523391096
1107	20-21	0.2944654218424745
1107	22-23	0.254632806033122
1107	24-25	0.3111804062043362
1107	26-27	0.2741980428951578
1107	28-29	0.32816942603083277
1107	30-31	0.3061301022163718
1107	32-33	0.3297567285411276
1107	34-35	0.3281354102733971
1107	36-37	0.2945758195528825
1107	38-39	0.25585614012732805
1107	40-41	0.284591086623486
1107	42-43	0.33098768693589875
1107	44-45	0.3203486498005077
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2216	58-59	-0.7086768811270474
2216	60-61	-0.6966712478904782
2216	62-63	-0.6252023156164981
2216	64-65	-0.7134688572128525
2216	66-67	-0.6956512019200147
2216	68-69	-0.7427954754263979
2216	70-71	-0.8095071219239429
2216	72-73	-0.6649363346087895
2216	74-75	-0.5960448035867429
2216	76-77	-0.6135417836941954
2216	78-79	-0.6317026016788247
2216	80-81	-0.644878617526409
2216	82-83	-0.6260531555075559
2216	84-85	-0.591236426763551
2216	86-87	-0.6071857788483968
2216	88-89	-0.5983613773389962
2216	90-91	-0.6381776547124609
2216	92-93	-0.6605285237834799
2216	94-95	-0.6396299191558334
2216	96-97	-0.6018549668368074
2216	98-99	-0.5946784217842023
2216	100	-0.7627863619384208
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1204.0
1	326.0
2	223.0
3	201.0
4	193.0
5	231.0
6	360.0
7	806.0
8	2375.0
9	3433.0
10	4007.0
11	4906.0
12	6558.0
13	8127.0
14	10298.0
15	10908.0
16	11740.0
17	12480.0
18	13862.0
19	15441.0
20	17145.0
21	19098.0
22	21403.0
23	24818.0
24	30088.0
25	35385.0
26	40029.0
27	41502.0
28	46511.0
29	56639.0
30	68180.0
31	80211.0
32	103387.0
33	139573.0
34	203700.0
35	307872.0
36	525398.0
37	947610.0
38	1051177.0
39	293605.0
40	379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.407825030505265	19.175442617990374	13.210674064739713	23.206058286764648
2	14.082644715536688	22.244827491683928	38.73635608613417	24.93617170664521
3	21.19383896730291	25.626599400365397	30.03057333234893	23.148988299982758
4	11.771643506827703	17.17002640654536	40.86150752995387	30.196822556673062
5	12.364834997809078	39.560747712999266	35.17945602595053	12.89496126324113
6	28.9257068970991	37.8870244180263	18.554659878376466	14.632608806498132
7	25.75170374959595	33.01219272861607	23.18446288115505	18.05164064063294
8	24.543258905014305	32.95491441921812	21.22960412484794	21.27222255091964
9	24.759950028917338	18.192985050758644	22.295856223720392	34.751208696603626
10-11	24.57039603360355	25.567406229528068	28.546845073701366	21.315352663167015
12-13	27.037132244906992	23.747686088056824	27.425934377962275	21.78924728907391
14-15	23.297618266028795	25.4223773859245	27.232841027668535	24.047163320378175
16-17	21.58694080861429	28.25334132894641	27.881490007427644	22.278227855011654
18-19	21.242185655913097	30.122481490214913	27.34217486983659	21.293157984035403
20-21	23.12476809318067	27.43135137318896	26.008901374283116	23.43497915934725
22-23	22.205228226753928	27.490400897571536	26.2344043518416	24.069966523832935
24-25	23.82002284398883	27.592154836984367	25.829904270003013	22.757918049023793
26-27	23.31456084238502	26.14237394758684	28.22961571161183	22.313449498416308
28-29	21.910227404588735	29.347607420906215	26.684888053307777	22.057277121197274
30-31	22.774762866603922	27.180305076222155	26.465023763544703	23.57990829362922
32-33	22.81860744999318	27.84491530906473	26.824864961327677	22.51161227961441
34-35	23.829907285848133	27.312367094022182	26.72096969067263	22.13675592945706
36-37	23.35065468254261	26.34736314115819	28.147711572417816	22.154270603881375
38-39	22.934927964980638	27.690201313226066	27.421446953844676	21.953423767948628
40-41	22.634714409060408	28.006815989917914	27.10925305504982	22.249216545971855
42-43	22.347122123714158	27.883663823267067	25.69664212189582	24.07257193112295
44-45	22.973590995648507	26.75488708784221	26.48943212914142	23.78208978736786
46-47	23.9046587627299	27.880473158746376	26.63179833801836	21.583069740505366
48-49	23.34309835427387	27.26687370483387	26.987777468165262	22.40225047272699
50-51	21.028327185480283	27.632532001887107	28.600318278118507	22.738822534514103
52-53	22.460159176880158	28.553208270308932	25.788232475648236	23.198400077162677
54-55	21.872064624646555	27.038601835660707	26.894414815354633	24.19491872433811
56-57	23.411687665852277	26.126803291158573	26.88630974966341	23.575199293325742
58-59	24.501508415713694	26.45742159293125	26.491998974344668	22.549071017010387
60-61	22.95996204328821	27.12972812723436	28.29447961961506	21.61583020986237
62-63	21.960520145655792	27.34935633652853	27.651533055246563	23.038590462569115
64-65	21.16741389099998	29.101386016246945	26.70212178676198	23.029078305991103
66-67	22.866749335183954	26.684350994006735	25.95954213989582	24.489357530913495
68-69	22.168890575873537	26.934245363418107	26.720730355308316	24.176133705400044
70-71	23.430231341418445	26.86121559544202	26.2784540226581	23.43009904048144
72-73	22.9279311730417	25.641912244281144	28.561494297594525	22.86866228508263
74-75	21.77421585756104	28.83703649713347	26.720848394838644	22.66789925046685
76-77	22.276610230972572	26.454654059510467	26.70646603653336	24.562269672983593
78-79	22.552065410323436	26.79782586234623	27.31029188768663	23.339816839643696
80-81	23.36638383824777	26.40223295975414	27.128976831130192	23.102406370867897
82-83	22.57203818301245	25.687653739933047	28.709763904296377	23.03054417275813
84-85	22.197607326271843	27.101473522054842	27.591220279958726	23.109698871714592
86-87	21.8419341938704	27.537282422733572	27.326387698709798	23.29439568468623
88-89	21.872869338084488	27.134005427313433	26.2260615680036	24.767063666598474
90-91	22.60297227505072	25.786861994402404	26.795203785707645	24.814961944839233
92-93	23.53101197280368	26.806007628295287	27.074507266603685	22.588473132297345
94-95	22.403441817349602	26.334607394558024	27.711096214592608	23.550854573499766
96-97	21.009145537774167	26.55716345941078	28.957422162872543	23.476268839942506
98-99	22.13674892934868	27.472673523934887	26.640268132514194	23.75030941420224
100	22.14207783491632	25.108988684737778	26.500902527728066	26.248030952617835
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1673.0
1	1060.5
2	457.0
3	484.0
4	480.0
5	405.0
6	342.0
7	355.5
8	382.0
9	388.0
10	391.0
11	397.5
12	423.5
13	441.5
14	466.5
15	516.5
16	619.0
17	812.5
18	1052.0
19	1354.5
20	1802.5
21	2470.5
22	3511.0
23	5079.0
24	7504.5
25	11033.5
26	15291.0
27	20440.5
28	26617.0
29	33775.0
30	41393.5
31	49061.0
32	58339.0
33	67716.0
34	77065.0
35	86484.5
36	96126.0
37	105621.5
38	110648.5
39	112477.5
40	114069.5
41	115745.5
42	117492.0
43	123112.5
44	134663.0
45	147798.0
46	159129.5
47	206396.5
48	310442.0
49	314143.5
50	228298.5
51	178309.5
52	144304.5
53	128555.0
54	120120.0
55	108902.5
56	97760.5
57	87335.5
58	77003.5
59	68474.0
60	56301.0
61	43440.5
62	34510.5
63	27070.0
64	19829.0
65	13700.5
66	9716.0
67	6921.0
68	5119.0
69	3917.0
70	3174.0
71	2631.0
72	2077.0
73	1738.0
74	1355.0
75	1025.0
76	799.0
77	556.0
78	372.5
79	228.5
80	153.5
81	102.0
82	76.5
83	66.0
84	47.5
85	34.0
86	28.5
87	29.0
88	29.0
89	23.0
90	18.5
91	16.5
92	15.5
93	14.5
94	13.0
95	13.0
96	12.0
97	5.5
98	6.5
99	10.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09395901224326782
2	0.06187837762823903
3	0.07665709694527477
4	0.07999732781530398
5	0.0798531451878207
6	0.0874948244444343
7	0.0841305631364912
8	0.08562045028715172
9	0.07170682673501565
10-11	0.07826713628550468
12-13	0.06841465674081418
14-15	0.07689740132441356
16-17	0.07871169938691144
18-19	0.07811093843906446
20-21	0.07040918308766617
22-23	0.07347306392168576
24-25	0.07650089909883455
26-27	0.06994058954834552
28-29	0.07861557763525592
30-31	0.08277284339435703
32-33	0.0842507153260606
34-35	0.0867378656501471
36-37	0.08676189608806098
38-39	0.0886482854643005
40-41	0.08456311101894104
42-43	0.09007809652017632
44-45	0.08702623090511366
46-47	0.08316934561993604
48-49	0.09281756644235856
50-51	0.08902075725196563
52-53	0.09464387972381337
54-55	0.08637740908143891
56-57	0.09339429695229165
58-59	0.09725118223746927
60-61	0.09663840607066534
62-63	0.09740738008390948
64-65	0.0988732367966561
66-67	0.1015045697482259
68-69	0.09780388230948849
70-71	0.1009278392382928
72-73	0.09767171490096214
74-75	0.10256190901643658
76-77	0.0990174194241394
78-79	0.10646685517744195
80-81	0.10131232624491487
82-83	0.1059622159812505
84-85	0.1019010719738049
86-87	0.09856084110377568
88-89	0.09812829322132587
90-91	0.09776783665261767
92-93	0.09231292724616708
94-95	0.09174821195519092
96-97	0.08970562473251119
98-99	0.10429210054623589
100	0.24679259737553977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4161389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.28916016240729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.80088290209908	42.43502323445112
2	14.412290796133231	16.801606530459974
3	5.6426173155902335	9.867102733308357
4	2.7303249447092828	6.365933919903009
5	1.4696233489583017	4.283155538292192
6	0.8727793222898574	3.052414422203448
7	0.5640326997101396	2.3013894659167513
8	0.38754706816880063	1.8071834501570083
9	0.26103986353115133	1.3694214972726204
>10	0.8429764572153254	7.292033586716841
>50	0.012663037939026523	0.5000165863441902
>100	0.002772821441861852	0.2704274511786107
>500	6.420317339841797E-5	0.02249151783350271
>1k	1.9260952019290226E-4	0.21439917748180332
>5k	0.0	0.0
>10k+	1.9260952019290226E-4	3.417400888480683
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	68479	1.6455803579045363	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	11885	0.2856017546064547	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	10953	0.2632053864707193	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4030437913879236E-5
2	0.0	0.0	0.0	4.806087582775847E-5	2.4030437913879236E-5
3	0.0	0.0	0.0	7.20913137416377E-5	2.4030437913879236E-5
4	0.0	0.0	0.0	1.2015218956939618E-4	2.4030437913879236E-5
5	0.0	0.0	0.0	1.2015218956939618E-4	2.4030437913879236E-5
6	0.0	0.0	0.0	1.2015218956939618E-4	7.20913137416377E-5
7	0.0	0.0	0.0	1.2015218956939618E-4	7.20913137416377E-5
8	0.0	0.0	0.0	1.2015218956939618E-4	7.20913137416377E-5
9	0.0	0.0	0.0	2.1627394122491312E-4	7.20913137416377E-5
10-11	0.0	0.0	0.0	3.484413497512489E-4	7.20913137416377E-5
12-13	0.0	0.0	0.0	4.5657832036370547E-4	1.5619784644021502E-4
14-15	0.0	0.0	0.0	4.806087582775847E-4	2.042587222679735E-4
16-17	0.0	2.4030437913879236E-5	0.0	5.527000720192224E-4	2.4030437913879235E-4
18-19	0.0	2.4030437913879236E-5	0.0	6.007609478469809E-4	2.4030437913879235E-4
20-21	0.0	2.4030437913879236E-5	0.0	8.050196701149543E-4	2.4030437913879235E-4
22-23	0.0	2.4030437913879236E-5	0.0	0.0011054001440384449	2.883652549665508E-4
24-25	0.0	2.4030437913879236E-5	0.0	0.0014898871506605126	2.883652549665508E-4
26-27	0.0	2.4030437913879236E-5	0.0	0.002366998134517105	3.0038047392349045E-4
28-29	0.0	2.4030437913879236E-5	0.0	0.005394833311665889	3.123956928804301E-4
30-31	0.0	2.4030437913879236E-5	0.0	0.014574460594767757	3.123956928804301E-4
32-33	0.0	2.4030437913879236E-5	0.0	0.038208396283067984	3.123956928804301E-4
34-35	0.0	2.4030437913879236E-5	0.0	0.07389359658517865	3.6045656870818855E-4
36-37	0.0	2.4030437913879236E-5	0.0	0.12607569251516743	3.6045656870818855E-4
38-39	0.0	2.4030437913879236E-5	0.0	0.20869233806308424	3.844870066220678E-4
40-41	0.0	2.4030437913879236E-5	0.0	0.3020746197964189	4.08517444535947E-4
42-43	0.0	2.4030437913879236E-5	0.0	0.3948321101439928	4.08517444535947E-4
44-45	0.0	2.4030437913879236E-5	0.0	0.5097696946860771	4.08517444535947E-4
46-47	0.0	2.4030437913879236E-5	0.0	0.6532434242508931	4.08517444535947E-4
48-49	0.0	2.4030437913879236E-5	0.0	0.8006100847577575	4.08517444535947E-4
50-51	0.0	2.4030437913879236E-5	0.0	0.9582497574728054	4.5657832036370547E-4
52-53	0.0	2.4030437913879236E-5	0.0	1.1205152894862749	4.5657832036370547E-4
54-55	0.0	2.4030437913879236E-5	0.0	1.3021493544583311	4.5657832036370547E-4
56-57	0.0	2.4030437913879236E-5	0.0	1.5321566909510262	4.5657832036370547E-4
58-59	0.0	2.4030437913879236E-5	0.0	1.7734823637011585	4.5657832036370547E-4
60-61	0.0	2.4030437913879236E-5	0.0	2.0265709358101347	4.5657832036370547E-4
62-63	0.0	2.4030437913879236E-5	0.0	2.2878418720287863	4.806087582775847E-4
64-65	0.0	2.4030437913879236E-5	0.0	2.564336090665881	5.04639196191464E-4
66-67	0.0	2.4030437913879236E-5	0.0	2.8808650188674982	5.166544151484036E-4
68-69	0.0	2.4030437913879236E-5	0.0	3.2378371740781744	5.286696341053432E-4
70-71	0.0	2.4030437913879236E-5	0.0	3.618239006254883	5.286696341053432E-4
72-73	0.0	2.4030437913879236E-5	0.0	3.9895453176811877	5.527000720192223E-4
74-75	0.0	2.4030437913879236E-5	0.0	4.376831870320222	5.767305099331016E-4
76-77	0.0	2.4030437913879236E-5	0.0	4.780218816361556	5.767305099331016E-4
78-79	0.0	2.4030437913879236E-5	0.0	5.28470181470658	5.767305099331016E-4
80-81	0.0	2.4030437913879236E-5	0.0	5.817144227564402	5.767305099331016E-4
82-83	0.0	2.4030437913879236E-5	0.0	6.327586774512068	5.767305099331016E-4
84-85	0.0	2.4030437913879236E-5	0.0	6.844109022251946	5.767305099331016E-4
86-87	0.0	2.4030437913879236E-5	0.0	7.3698229124938806	5.887457288900413E-4
88	0.0	2.4030437913879236E-5	0.0	7.800880907793047	6.007609478469809E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1395	0.0	23.933128	1
CGTTAAC	280	7.6397555E-11	21.832357	1
CATGGGG	7635	0.0	21.303537	4
CTAACGC	475	0.0	19.793907	3
CTGTGCG	2165	0.0	19.534727	9
CTAGTAC	2415	0.0	18.882076	3
TACCTGG	3420	0.0	18.831703	2
GGCGTCG	775	0.0	18.797424	8
TAGTACT	2500	0.0	18.61572	4
ATGGGGG	5185	0.0	17.950651	5
GAGTACG	1155	0.0	17.91373	1
CGACGAG	710	0.0	17.869934	24-25
TACGGGC	870	0.0	17.83115	4
TGCGCGA	820	0.0	17.797752	94
CGATAAT	1030	0.0	17.797318	66-67
CTCGTCG	1245	0.0	17.362457	9
TTTGCGT	980	0.0	17.269173	3
TAACGCC	545	0.0	17.251156	4
ACGGGCG	900	0.0	17.235949	5
TCTAACG	555	0.0	16.94073	2
>>END_MODULE