FastQCFastQC Report
Mon 16 Jan 2017
ERR1146629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161389
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT729901.7539816633340453No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC181160.43533541324783626No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG122610.2946371992620733No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101030.2427795142439219No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90170.21668245866944907No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG85560.20560442679115076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82970.19938054337145603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42510.10215339157190063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT112950.023.15552112-13
GTACTAG14450.021.8041111
GTACTTT123500.021.13940614-15
CTAACGC4900.021.1121233
ATAAGCG2551.3413228E-820.2841973
GAGTACG11750.020.010761
ATACGGG9050.019.2246513
GGCGTCG9800.019.1826938
AGTACTT121000.018.97382412-13
TGATCGC8550.018.6891029
TAACGCC5050.018.6227554
CTAGTAC21250.018.58773
CATGGGG68150.018.3535924
ACTTTTT143750.018.25958616-17
TACGGGC9800.018.2331984
GTATAAG18850.018.2113821
GTGATCG8800.018.1581638
TAGTACT21350.017.8399014
CGACGAG6700.017.53652224-25
TCTAACG5400.017.4161432
Produced by FastQC (version 0.11.5)