FastQCFastQC Report
Mon 16 Jan 2017
ERR1146628_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146628_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988639
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4641789.30470214421208No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1146052.2973199704368263No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1119422.2439386774629315No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC864331.7325968064636468No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA796061.5957458537288427No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG732271.4678753062709087No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA727851.4590151742789967No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC615431.2336631293625377No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG409630.8211257619563171No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT350960.7035185348148062No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG196660.3942157369976059No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118650.23784042100460667No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG112000.2245101319217526No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA105740.21196161919112608No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75660.1516646123321411No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC71850.1440272587373029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATG20600.026.7106881
TAGCGTG950.001304081624.7404675
CTAACGC5400.024.3756543
GTACTAG8600.024.0613881
GTACGCA56000.023.9248313
GAGTACG57100.023.555721
TACGCAG57400.023.3403634
TCTAACG5650.023.2970872
AGTACGC58900.022.7468682
TCTCGGG19100.022.67346894
GTGATCG8500.022.6661788
GGGCACC13250.022.38144594
AGTAAGG13750.022.25225894
TAGTACT15050.022.1766784
TATGTAC24450.022.1102474
CGCAGTG60850.022.0871966
TAGGCAG31750.021.6164194
GTAGGCA33600.020.9866923
TTATGTA25350.020.9552353
AGTAGGC32550.020.9415592
Produced by FastQC (version 0.11.5)