##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146628_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4988639
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72092368279204	33.0	31.0	34.0	30.0	34.0
2	31.816105555042167	33.0	31.0	34.0	30.0	34.0
3	32.02288459838445	34.0	31.0	34.0	30.0	34.0
4	35.366106868025526	37.0	35.0	37.0	33.0	37.0
5	35.53496835509645	37.0	35.0	37.0	35.0	37.0
6	35.71182861698351	37.0	35.0	37.0	35.0	37.0
7	35.69415566049177	37.0	36.0	37.0	35.0	37.0
8	35.720557651094815	37.0	36.0	37.0	35.0	37.0
9	37.38710077037044	39.0	38.0	39.0	35.0	39.0
10-11	37.353531694716736	39.0	37.5	39.0	35.0	39.0
12-13	37.37938884733892	39.0	38.0	39.0	35.0	39.0
14-15	38.56395161085017	40.0	38.0	41.0	34.5	41.0
16-17	38.74739272575145	40.0	38.5	41.0	35.5	41.0
18-19	38.860489303796086	41.0	39.0	41.0	36.0	41.0
20-21	38.84633363929521	41.0	39.0	41.0	35.0	41.0
22-23	38.76429593321946	40.0	39.0	41.0	35.0	41.0
24-25	38.68178956224333	40.0	39.0	41.0	34.5	41.0
26-27	38.50903623212663	40.0	38.5	41.0	34.5	41.0
28-29	38.561962791855656	40.0	38.0	41.0	34.5	41.0
30-31	38.64968020335807	40.0	38.0	41.0	34.5	41.0
32-33	38.467912490761506	40.0	38.0	41.0	34.0	41.0
34-35	38.120022414931206	40.0	38.0	41.0	33.0	41.0
36-37	38.02543268815403	40.0	38.0	41.0	33.0	41.0
38-39	37.89613790454671	40.0	38.0	41.0	33.0	41.0
40-41	38.0999940063813	40.0	38.0	41.0	33.0	41.0
42-43	37.91541069618387	40.0	38.0	41.0	33.0	41.0
44-45	37.64163582492139	40.0	37.0	41.0	32.0	41.0
46-47	37.34817201645579	40.0	37.0	41.0	31.0	41.0
48-49	37.10907904941608	39.0	36.5	41.0	30.5	41.0
50-51	36.782088862312946	39.0	36.0	41.0	29.5	41.0
52-53	36.87582595172752	39.0	36.0	41.0	30.5	41.0
54-55	36.6582747318457	39.0	35.5	40.5	29.5	41.0
56-57	35.84595928869578	38.5	34.5	40.0	27.5	41.0
58-59	35.58583222798843	38.0	34.0	40.0	28.0	41.0
60-61	35.04222975444806	38.0	33.0	40.0	26.5	41.0
62-63	35.09458341242972	37.0	33.5	40.0	27.0	41.0
64-65	34.856360321923475	36.5	33.5	39.5	26.5	40.5
66-67	34.126392288558066	36.0	32.5	39.0	26.0	40.0
68-69	33.41648092796452	35.5	31.5	38.5	25.0	39.0
70-71	32.739811800372806	35.0	31.0	37.0	24.0	39.0
72-73	32.42963641987323	35.0	31.0	37.0	24.5	38.5
74-75	32.33065491409581	35.0	31.0	36.0	25.5	37.0
76-77	32.12180306091501	34.0	31.0	35.5	25.0	37.0
78-79	31.590538822312055	34.0	30.5	35.0	23.0	36.0
80-81	30.81748929918561	34.0	29.0	35.0	20.0	36.0
82-83	30.677416024691304	34.0	29.0	35.0	20.5	36.0
84-85	30.6963890351657	34.0	29.0	35.0	20.0	35.0
86-87	31.058391577342036	34.0	30.0	35.0	22.5	35.5
88-89	30.746758083717822	34.0	29.5	35.0	20.0	35.0
90-91	30.18465777940637	34.0	29.0	35.0	19.0	35.0
92-93	29.788830380390323	34.0	28.5	35.0	18.5	35.0
94-95	29.77622503853255	34.0	28.0	35.0	18.5	35.0
96-97	29.886071631962146	34.0	29.0	35.0	18.5	35.0
98-99	30.064931838122583	34.0	29.0	35.0	18.5	35.0
100	28.464595654245578	32.0	25.0	34.0	11.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.244833124327144
1101	2	0.30245601829325963
1101	3	0.248657977371451
1101	4	0.20053812671148563
1101	5	0.18305427370422223
1101	6	0.2276676832301021
1101	7	0.2687858057014765
1101	8	0.280729138418252
1101	9	0.3644480772025105
1101	10-11	0.3628685930963158
1101	12-13	0.35123361558726174
1101	14-15	0.5056559238270566
1101	16-17	0.46947372776509155
1101	18-19	0.4415385375572356
1101	20-21	0.4437434370539961
1101	22-23	0.5559998781772961
1101	24-25	0.5731685667695885
1101	26-27	0.5610287101773253
1101	28-29	0.5182183458230583
1101	30-31	0.577218412741729
1101	32-33	0.5729927001220005
1101	34-35	0.5672197146546409
1101	36-37	0.5717479636853326
1101	38-39	0.6079637890312171
1101	40-41	0.5056786206986459
1101	42-43	0.5589647748206374
1101	44-45	0.5443857514182397
1101	46-47	0.6103663376372879
1101	48-49	0.724617790642526
1101	50-51	0.6639886704412845
1101	52-53	0.6909326184611118
1101	54-55	0.6726654611847351
1101	56-57	0.6981418333019818
1101	58-59	0.7983277703550016
1101	60-61	0.7453682228655794
1101	62-63	0.7341978776969498
1101	64-65	0.8078349168229337
1101	66-67	0.7743211042934419
1101	68-69	0.77974449664584
1101	70-71	0.6821593275503801
1101	72-73	0.7187965579143025
1101	74-75	0.7185296664039527
1101	76-77	0.7821292251689513
1101	78-79	0.7581455787780236
1101	80-81	0.6952847464402261
1101	82-83	0.7330676484833276
1101	84-85	0.7025522037005736
1101	86-87	0.6905818652784461
1101	88-89	0.7082134979379511
1101	90-91	0.6612884887576769
1101	92-93	0.6741665591874657
1101	94-95	0.78302679886243
1101	96-97	0.635812892627083
1101	98-99	0.745389671627251
1101	100	0.8822152103219629
1102	1	0.23515572445366573
1102	2	0.3323056265742359
1102	3	0.29946655367022856
1102	4	0.2984005441817459
1102	5	0.29184768249762527
1102	6	0.33359985975051387
1102	7	0.29900063591630754
1102	8	0.32293091787493466
1102	9	0.3977744654308566
1102	10-11	0.425115832794539
1102	12-13	0.3992920058025149
1102	14-15	0.5280784217397567
1102	16-17	0.5339121480858608
1102	18-19	0.5160299218525424
1102	20-21	0.5088326590059609
1102	22-23	0.5066832916058033
1102	24-25	0.5935893646413675
1102	26-27	0.5823849834943786
1102	28-29	0.5560521826764955
1102	30-31	0.575078490013567
1102	32-33	0.545844133757754
1102	34-35	0.6043606263053505
1102	36-37	0.6374533372338433
1102	38-39	0.6072597667585669
1102	40-41	0.5566854344505572
1102	42-43	0.6300315429462273
1102	44-45	0.6430600667592117
1102	46-47	0.714075325660005
1102	48-49	0.7327070261827373
1102	50-51	0.7741199933177114
1102	52-53	0.7187996257398837
1102	54-55	0.6535491352056653
1102	56-57	0.8214383575788986
1102	58-59	0.823210722688934
1102	60-61	0.7759843593640667
1102	62-63	0.7128252229909648
1102	64-65	0.8016254645350571
1102	66-67	0.7576772356887957
1102	68-69	0.736825635099521
1102	70-71	0.8096870060584465
1102	72-73	0.76183507154105
1102	74-75	0.7581468099426729
1102	76-77	0.6618462725330616
1102	78-79	0.6407682466948081
1102	80-81	0.6774624746963838
1102	82-83	0.7684731142809511
1102	84-85	0.7508826608545611
1102	86-87	0.6939990834015433
1102	88-89	0.6843581191806116
1102	90-91	0.6838781221316239
1102	92-93	0.7700427521224817
1102	94-95	0.8129354405357745
1102	96-97	0.7723254232572607
1102	98-99	0.7124363563209926
1102	100	0.755649730815307
1103	1	0.24225450802303783
1103	2	0.22656235024272675
1103	3	0.2543626379780761
1103	4	0.2321295602637008
1103	5	0.178553133908963
1103	6	0.17320535229718104
1103	7	0.21246951997342478
1103	8	0.20959523395101343
1103	9	0.2961897756108769
1103	10-11	0.33731539793527787
1103	12-13	0.3507591660931979
1103	14-15	0.4360547591278561
1103	16-17	0.4425290214086175
1103	18-19	0.3894369070242263
1103	20-21	0.419027366259499
1103	22-23	0.461347255093294
1103	24-25	0.48543183589560357
1103	26-27	0.4663255527290602
1103	28-29	0.4467262145270041
1103	30-31	0.5103569370567271
1103	32-33	0.4606763603546824
1103	34-35	0.5113644068385454
1103	36-37	0.48391080133836084
1103	38-39	0.48380283153692005
1103	40-41	0.4457169911291743
1103	42-43	0.5478147401411846
1103	44-45	0.5095347685280345
1103	46-47	0.5768370737158648
1103	48-49	0.603175773122075
1103	50-51	0.6203345630616894
1103	52-53	0.6071818317691466
1103	54-55	0.5310010950105664
1103	56-57	0.5745088165317114
1103	58-59	0.6622148558107099
1103	60-61	0.7258110033083511
1103	62-63	0.6000311543145429
1103	64-65	0.5540847699355353
1103	66-67	0.602856566162238
1103	68-69	0.6229248300222494
1103	70-71	0.6586419726604831
1103	72-73	0.5521369265487905
1103	74-75	0.5726421185556205
1103	76-77	0.5867311915356268
1103	78-79	0.5515662721987162
1103	80-81	0.5804007129287427
1103	82-83	0.5875432598216825
1103	84-85	0.5806587671012515
1103	86-87	0.4619193983806831
1103	88-89	0.4465185078115894
1103	90-91	0.48162841105838083
1103	92-93	0.493222513537539
1103	94-95	0.5869721881019387
1103	96-97	0.5358374014751206
1103	98-99	0.6043562011820178
1103	100	0.8006439522801188
1104	1	0.14581660526410545
1104	2	0.11196126419914165
1104	3	0.16683796867253875
1104	4	0.11076751586222855
1104	5	0.10135264184994952
1104	6	0.17641341980836955
1104	7	0.16759848667726374
1104	8	0.2054304788050132
1104	9	0.24971443092358214
1104	10-11	0.26975691040039607
1104	12-13	0.23033826173653438
1104	14-15	0.35830669205422083
1104	16-17	0.31174414908043246
1104	18-19	0.32248737416823303
1104	20-21	0.3145655966172569
1104	22-23	0.32686468832833526
1104	24-25	0.4221329549111843
1104	26-27	0.40664660602054425
1104	28-29	0.3424405546578555
1104	30-31	0.36268191811365114
1104	32-33	0.32381519000540493
1104	34-35	0.3611804510852039
1104	36-37	0.41606691557805675
1104	38-39	0.3927559223478454
1104	40-41	0.3518810886121173
1104	42-43	0.39692419084371977
1104	44-45	0.4044922963990345
1104	46-47	0.4973241997005431
1104	48-49	0.4375089053362231
1104	50-51	0.46561821829121186
1104	52-53	0.4461729220005779
1104	54-55	0.45440537088655475
1104	56-57	0.5153624192026101
1104	58-59	0.5471108607575133
1104	60-61	0.5627510221498113
1104	62-63	0.4916688608184643
1104	64-65	0.5940325478017812
1104	66-67	0.5111024415254235
1104	68-69	0.5610001672022733
1104	70-71	0.5277682014337159
1104	72-73	0.48615309892980463
1104	74-75	0.4266886176214939
1104	76-77	0.3721434653140321
1104	78-79	0.5162492995943531
1104	80-81	0.44296966787860015
1104	82-83	0.48650803464649073
1104	84-85	0.4252765634724689
1104	86-87	0.35651829887732944
1104	88-89	0.4406768451345471
1104	90-91	0.49127038226624364
1104	92-93	0.4667194697081065
1104	94-95	0.38263938571880374
1104	96-97	0.35410237705263725
1104	98-99	0.4108702415997527
1104	100	0.5719922093008805
1105	1	0.17317742222839172
1105	2	0.1371381524144475
1105	3	0.11829034980853947
1105	4	0.16558293954104641
1105	5	0.1725847339990807
1105	6	0.15783687124298496
1105	7	0.1668074725480153
1105	8	0.19237614385685475
1105	9	0.23082205719124715
1105	10-11	0.2705728128132776
1105	12-13	0.2316245122835383
1105	14-15	0.3663399583083944
1105	16-17	0.3192971678623451
1105	18-19	0.3060950031309986
1105	20-21	0.3387574851389701
1105	22-23	0.33475148584124526
1105	24-25	0.44390541409419626
1105	26-27	0.3903647965965007
1105	28-29	0.3235938630021451
1105	30-31	0.37971286383857716
1105	32-33	0.3866080601102482
1105	34-35	0.429497732356694
1105	36-37	0.42657452917456595
1105	38-39	0.4421349301285815
1105	40-41	0.3787982701065573
1105	42-43	0.4279554854399805
1105	44-45	0.42355504240536135
1105	46-47	0.4298947698534903
1105	48-49	0.4508653825965183
1105	50-51	0.4597912709120777
1105	52-53	0.39938365363775574
1105	54-55	0.4122189526872617
1105	56-57	0.45311559866270557
1105	58-59	0.4684596344876226
1105	60-61	0.535816115138914
1105	62-63	0.45723078415350926
1105	64-65	0.4523767299804575
1105	66-67	0.5182802183799566
1105	68-69	0.5480125983661068
1105	70-71	0.5139767648774196
1105	72-73	0.4514062354754884
1105	74-75	0.4610006979983936
1105	76-77	0.36974193771035857
1105	78-79	0.43428421027889286
1105	80-81	0.5276140571161818
1105	82-83	0.5145672265934778
1105	84-85	0.5175391101735016
1105	86-87	0.3491261054684358
1105	88-89	0.3717498475600607
1105	90-91	0.5089992676103066
1105	92-93	0.6001393802686756
1105	94-95	0.5742422156482974
1105	96-97	0.5466475721440212
1105	98-99	0.5017872557487344
1105	100	0.6387759638878521
1106	1	0.0687131596655135
1106	2	0.17234873297763542
1106	3	0.13996471971986324
1106	4	0.16201162672665248
1106	5	0.14229931419926345
1106	6	0.12675768232009688
1106	7	0.12738787367854343
1106	8	0.186543285899063
1106	9	0.24416055483165167
1106	10-11	0.20314570733176396
1106	12-13	0.2300163519220746
1106	14-15	0.27244823697353837
1106	16-17	0.22088081667217807
1106	18-19	0.2749679660387443
1106	20-21	0.2925902004840992
1106	22-23	0.3530978757961236
1106	24-25	0.35092613020423613
1106	26-27	0.36640015643418167
1106	28-29	0.31032318740706444
1106	30-31	0.30285598212930154
1106	32-33	0.360986114938342
1106	34-35	0.39889305466131475
1106	36-37	0.3831731199021533
1106	38-39	0.371252101017447
1106	40-41	0.371977495330853
1106	42-43	0.40746249128709877
1106	44-45	0.4289866401857907
1106	46-47	0.5066337935401606
1106	48-49	0.47540946773628434
1106	50-51	0.5242587179509144
1106	52-53	0.4695320275292616
1106	54-55	0.3799387382496846
1106	56-57	0.4872941011000833
1106	58-59	0.5167089830070495
1106	60-61	0.5379457435680948
1106	62-63	0.5248735912893281
1106	64-65	0.5515595837246892
1106	66-67	0.6165233681102862
1106	68-69	0.5087417704347743
1106	70-71	0.4756283410714772
1106	72-73	0.4551622559987507
1106	74-75	0.3968083475472213
1106	76-77	0.40527886628733967
1106	78-79	0.4002168403805406
1106	80-81	0.36736117893332576
1106	82-83	0.3991820346497974
1106	84-85	0.3508614504473151
1106	86-87	0.40465538482071395
1106	88-89	0.426672131761169
1106	90-91	0.4734118573914614
1106	92-93	0.39648120337711035
1106	94-95	0.3672195143051482
1106	96-97	0.4133160349739775
1106	98-99	0.42548397047155007
1106	100	0.5047644641212194
1107	1	0.06350215700439676
1107	2	0.09046067380480238
1107	3	0.13796314738313242
1107	4	0.11768093243449584
1107	5	0.0887133546580543
1107	6	0.10145738294472295
1107	7	0.14237215801123426
1107	8	0.14987503816902148
1107	9	0.16127338423697068
1107	10-11	0.15871791624157083
1107	12-13	0.22625952011128447
1107	14-15	0.21803219990889744
1107	16-17	0.25108061585659414
1107	18-19	0.23984617346707893
1107	20-21	0.2331368531470588
1107	22-23	0.2703321916987491
1107	24-25	0.30087056519482047
1107	26-27	0.2603943133013331
1107	28-29	0.267311173122053
1107	30-31	0.2926869982807858
1107	32-33	0.304260920051739
1107	34-35	0.309595724399756
1107	36-37	0.2967328301979464
1107	38-39	0.33766593550166846
1107	40-41	0.31668329434278775
1107	42-43	0.35487967603435777
1107	44-45	0.29842925374515517
1107	46-47	0.3601170256700357
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2216	92-93	-2.5584586392221738
2216	94-95	-2.583627240705969
2216	96-97	-2.18642619762549
2216	98-99	-2.249406875951827
2216	100	-2.380430045379544
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1520.0
1	366.0
2	252.0
3	288.0
4	250.0
5	273.0
6	394.0
7	1135.0
8	2648.0
9	2972.0
10	2982.0
11	3278.0
12	4170.0
13	5497.0
14	7194.0
15	7880.0
16	8850.0
17	10098.0
18	11253.0
19	13335.0
20	15596.0
21	18602.0
22	22641.0
23	27710.0
24	34167.0
25	41951.0
26	49488.0
27	57532.0
28	69229.0
29	86066.0
30	108272.0
31	137229.0
32	184713.0
33	256418.0
34	379418.0
35	572261.0
36	847586.0
37	1106312.0
38	749491.0
39	139158.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.672649027791074	16.45131250161779	12.901324751673021	17.974713718918117
2	12.842942489485262	28.430247298904156	29.467318866510585	29.259491345099995
3	34.94529224846963	20.605439538801388	29.340283037056103	15.108985175672881
4	10.591689313656767	13.456926448006348	46.88065380619901	29.070730432137875
5	10.076613932227927	44.11147258927325	34.079437099857316	11.732476378641508
6	26.13000930564033	38.102403776508936	14.34047728825856	21.427109629592177
7	24.601266701496144	33.39845803329024	22.813627794884884	19.186647470328737
8	22.485112837469103	19.7931446823537	20.078067317261084	37.64367516291612
9	17.260113048932972	26.7488491793551	29.25215866008709	26.738879111624836
10-11	27.96534461188581	19.7201600781934	26.196473753772647	26.11802155614814
12-13	36.83585503916734	20.47740615028692	22.269331643224906	20.41740716732083
14-15	24.94240303909732	24.32995134250471	33.68544516171485	17.042200456683112
16-17	19.208240751338312	27.232202013202606	30.857948834051168	22.70160840140791
18-19	17.399830677130982	38.100634760139314	23.69431805415122	20.80521650857849
20-21	24.12068486841366	25.08573693645116	19.900888984107574	30.89268921102761
22-23	19.753135086305523	26.606963060236627	24.505390496813206	29.134511356644644
24-25	29.99920957703745	25.61433819229466	21.222525529758922	23.163926700908966
26-27	25.927286306219905	18.801142911133514	34.51510780514182	20.756462977504768
28-29	18.698599240111108	36.757290460542585	24.282630037709563	20.261480261636745
30-31	22.503519504081464	24.126855024920918	23.354299552982354	30.015325918015268
32-33	24.702196772478803	26.028785045491436	26.33620097709011	22.93281720493964
34-35	30.30130969978376	22.624114506814845	25.062771391152864	22.011804402248526
36-37	25.25208304450228	20.067299055349423	33.957522725444214	20.723095174704078
38-39	22.23409217903716	28.192875150102175	29.433990656296366	20.1390420145643
40-41	21.47606140861341	30.693342244007138	25.448968653374422	22.381627694005026
42-43	19.47121637769164	30.225519602786783	18.93085977624303	31.372404243278545
44-45	24.235441004472204	22.032111482827986	22.60177826883774	31.130669243862062
46-47	30.359817292600884	27.969501819991844	24.497312896352053	17.173367991055226
48-49	26.34328106653483	25.59625804287231	26.12106624589981	21.93939464469305
50-51	14.685298409816555	28.09496298286324	35.924333940830564	21.295404666489638
52-53	22.841369490959522	32.37917672590919	21.305565944755497	23.473887838375795
54-55	18.52012172397829	23.847687530314378	26.902794866406076	30.729395879301258
56-57	27.112005096384877	20.09190547610568	25.58614947054781	27.20993995696163
58-59	34.62160335124573	21.614918958789445	22.56962147994893	21.193856210015895
60-61	24.325345205467908	24.853399163003274	33.78040443672071	17.040851194808106
62-63	19.524047394004928	26.413703203667225	30.563934241364944	23.498315160962903
64-65	17.577488652996653	36.511982489711976	23.88724421147068	22.02328464582069
66-67	23.63195143488169	24.655660801873065	20.599109721100326	31.113278042144927
68-69	19.45100671342942	26.156038700414907	25.0565773097824	29.33637727637327
70-71	28.96047225531147	25.203822035002784	21.81640470782473	24.019301001861013
72-73	25.028099066427767	18.90435361713215	34.25094354974556	21.816603766694527
74-75	18.82680527865587	35.28240724622748	24.682635419038032	21.208152056078617
76-77	22.124808122560776	23.547199341847843	24.173075958383915	30.15491657720747
78-79	23.95555989895836	25.14148277885382	27.10306761072801	23.799889711459816
80-81	28.9229710361946	22.44711222276902	25.56391807241729	23.06599866861909
82-83	24.197784772659865	20.05242081292695	33.51998243743235	22.229811976980834
84-85	21.598796350725788	27.391769839399387	29.3224466322592	21.686987177615624
86-87	21.102255004697824	29.592566154512944	26.057727277515617	23.247451563273618
88-89	19.555523896998885	28.975531893013923	20.4413057883825	31.027638421604692
90-91	23.56368923393214	21.533259372561435	23.73244861321546	31.170602780290956
92-93	28.711513481692887	26.772104354358273	25.25326955079215	19.263112613156693
94-95	24.76445893346584	24.905058754473185	26.71566141556682	23.614820896494155
96-97	15.350036275268291	26.67906539820935	35.07219540812887	22.89870291839349
98-99	22.72408625278837	30.520637277019414	22.632091224874316	24.123185245317902
100	21.547693733499614	17.37979960606045	23.762327691635594	37.310178968804344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1737.0
1	1064.0
2	375.5
3	363.0
4	348.0
5	306.0
6	270.5
7	272.5
8	286.5
9	283.5
10	272.0
11	275.0
12	297.5
13	302.0
14	310.5
15	378.5
16	423.0
17	440.5
18	526.0
19	614.5
20	712.0
21	926.5
22	1259.0
23	1760.0
24	2424.5
25	3314.0
26	4678.5
27	6174.5
28	8072.5
29	11243.5
30	14704.5
31	18196.5
32	23444.5
33	27761.5
34	31372.5
35	35866.5
36	39802.0
37	45716.5
38	49416.0
39	49049.0
40	48806.5
41	48423.5
42	49456.0
43	54031.5
44	61385.0
45	77867.5
46	137587.0
47	452037.5
48	1111978.0
49	1133048.0
50	524784.5
51	213553.5
52	117238.5
53	91001.5
54	80716.0
55	70071.5
56	62189.0
57	53964.0
58	45178.0
59	39563.5
60	32896.0
61	24796.0
62	18860.0
63	14856.0
64	10975.5
65	7307.5
66	5188.0
67	3959.0
68	3026.5
69	2348.0
70	1807.0
71	1357.0
72	1005.5
73	805.0
74	638.5
75	487.5
76	327.0
77	220.5
78	151.5
79	100.5
80	78.5
81	52.5
82	45.0
83	40.5
84	33.5
85	31.0
86	27.5
87	24.0
88	22.5
89	16.0
90	13.5
91	15.0
92	12.5
93	8.5
94	6.5
95	7.0
96	7.0
97	6.0
98	6.0
99	8.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09998719089515196
2	0.06524825708975936
3	0.08142501391662135
4	0.08348970530840175
5	0.08352979640338778
6	0.0915079243056072
7	0.08729835933207433
8	0.08978400722120802
9	0.07452934557902466
10-11	0.08168560603403052
12-13	0.07125189856391693
14-15	0.08020223551954751
16-17	0.08205644866265127
18-19	0.08176578822400257
20-21	0.07262501856718837
22-23	0.07713526675311644
24-25	0.07950064135729204
26-27	0.07315622557575323
28-29	0.08207649421014429
30-31	0.0856345788901542
32-33	0.08718810882086275
34-35	0.08983412108994057
36-37	0.08956350619878488
38-39	0.09088651233332377
40-41	0.08728833655832784
42-43	0.09252022445400439
44-45	0.08839084167044359
46-47	0.08571476108012625
48-49	0.09467512080950335
50-51	0.09077626182211221
52-53	0.09684003993874883
54-55	0.08843093276542961
56-57	0.09449471088206624
58-59	0.09902500461548731
60-61	0.09758172519599033
62-63	0.09879448081931766
64-65	0.10132021980343738
66-67	0.10312431907780858
68-69	0.09965643936151725
70-71	0.10150062973087448
72-73	0.09887466300928971
74-75	0.10294390915037148
76-77	0.10000723644264498
78-79	0.10718354244514386
80-81	0.10206190506067887
82-83	0.10650199383038139
84-85	0.10278354477042735
86-87	0.0991553006741919
88-89	0.09835347877447136
90-91	0.09854391147565499
92-93	0.09263047496521597
94-95	0.09292113540386465
96-97	0.09074619350087268
98-99	0.10458764404479859
100	0.2453775468619798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4988639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.99531491629295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.80517907401534	32.758919599724216
2	14.880666696634476	13.391205683589218
3	5.593234554259567	7.550080505088021
4	2.6284394205690518	4.730698378676122
5	1.4502620737915104	3.262749936070255
6	0.8262676161786867	2.230690295705681
7	0.5118642452136091	1.6122045035443855
8	0.35301672520139044	1.270727897692401
9	0.2408121039760202	0.9751874808650499
>10	0.7036999550396601	4.491630674366466
>50	0.004492401891247556	0.13645661919513968
>100	7.132822771439417E-4	0.05062833536137734
>500	2.503427689493661E-4	0.0955659116419668
>1k	3.0041132273005724E-4	0.21904463905502977
>5k	1.0013710757668571E-4	0.3323179544071524
>10k+	7.009597530368E-4	26.891891585017486
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	464178	9.30470214421208	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	114605	2.2973199704368263	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	111942	2.2439386774629315	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	86433	1.7325968064636468	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	79606	1.5957458537288427	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	73227	1.4678753062709087	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	72785	1.4590151742789967	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	61543	1.2336631293625377	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	40963	0.8211257619563171	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	35096	0.7035185348148062	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	19666	0.3942157369976059	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	11865	0.23784042100460667	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	11200	0.2245101319217526	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	10574	0.21196161919112608	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	7566	0.1516646123321411	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	7185	0.1440272587373029	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.0045547493013625E-5	0.0
9	0.0	0.0	0.0	8.01821899720545E-5	0.0
10-11	0.0	0.0	0.0	4.710703660858202E-4	0.0
12-13	0.0	0.0	0.0	7.717535784810246E-4	0.0
14-15	0.0	0.0	0.0	0.0013731200032714333	0.0
16-17	0.0	0.0	0.0	0.0026760805903173193	0.0
18-19	0.0	0.0	0.0	0.004520270959674572	0.0
20-21	0.0	0.0	0.0	0.00751708030988011	0.0
22-23	0.0	0.0	0.0	0.011205461048594616	0.0
24-25	0.0	0.0	0.0	0.014713431859872	0.0
26-27	0.0	0.0	0.0	0.018121174933684318	0.0
28-29	0.0	0.0	0.0	0.028705224009995513	0.0
30-31	0.0	0.0	0.0	0.046816376169933324	0.0
32-33	0.0	0.0	0.0	0.10625142448671873	0.0
34-35	0.0	0.0	0.0	0.17666141005592909	0.0
36-37	0.0	0.0	0.0	0.24787321752485997	0.0
38-39	0.0	0.0	0.0	0.35886541399367644	0.0
40-41	0.0	0.0	0.0	0.47663300551513144	0.0
42-43	0.0	0.0	0.0	0.6118803144504944	0.0
44-45	0.0	0.0	0.0	0.7837909297505792	0.0
46-47	0.0	0.0	0.0	1.017221330306723	0.0
48-49	0.0	0.0	0.0	1.1817251158081392	0.0
50-51	0.0	0.0	0.0	1.3785222783208004	0.0
52-53	0.0	0.0	0.0	1.5730643167404978	0.0
54-55	0.0	0.0	0.0	1.8297174840672978	0.0
56-57	0.0	0.0	0.0	2.25487352362037	0.0
58-59	0.0	0.0	0.0	2.5603676674138978	0.0
60-61	0.0	0.0	0.0	2.8757643116689744	0.0
62-63	0.0	0.0	0.0	3.1843354470026792	0.0
64-65	0.0	0.0	0.0	3.486742576482283	0.0
66-67	0.0	0.0	0.0	3.895571517602296	0.0
68-69	0.0	0.0	0.0	4.386176269720059	0.0
70-71	0.0	0.0	0.0	4.836820222910497	0.0
72-73	0.0	0.0	0.0	5.1980810798295884	0.0
74-75	0.0	0.0	0.0	5.674563743738522	1.0022773746506812E-5
76-77	0.0	0.0	0.0	6.072357610963632	2.0045547493013625E-5
78-79	0.0	0.0	0.0	6.8880410067755955	2.0045547493013625E-5
80-81	0.0	0.0	0.0	7.661628752852231	2.0045547493013625E-5
82-83	0.0	0.0	0.0	8.180347385328945	2.0045547493013625E-5
84-85	0.0	0.0	0.0	8.759423161307122	2.0045547493013625E-5
86-87	0.0	0.0	0.0	9.30662651677141	2.0045547493013625E-5
88	0.0	0.0	0.0	9.816986155943535	2.0045547493013625E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTATG	2060	0.0	26.710688	1
TAGCGTG	95	0.0013040816	24.740467	5
CTAACGC	540	0.0	24.375654	3
GTACTAG	860	0.0	24.061388	1
GTACGCA	5600	0.0	23.924831	3
GAGTACG	5710	0.0	23.55572	1
TACGCAG	5740	0.0	23.340363	4
TCTAACG	565	0.0	23.297087	2
AGTACGC	5890	0.0	22.746868	2
TCTCGGG	1910	0.0	22.673468	94
GTGATCG	850	0.0	22.666178	8
GGGCACC	1325	0.0	22.381445	94
AGTAAGG	1375	0.0	22.252258	94
TAGTACT	1505	0.0	22.176678	4
TATGTAC	2445	0.0	22.110247	4
CGCAGTG	6085	0.0	22.087196	6
TAGGCAG	3175	0.0	21.616419	4
GTAGGCA	3360	0.0	20.986692	3
TTATGTA	2535	0.0	20.955235	3
AGTAGGC	3255	0.0	20.941559	2
>>END_MODULE