FastQCFastQC Report
Mon 16 Jan 2017
ERR1146628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988639
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4738309.498181768614646No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1241372.4883941291402327No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1105242.215514091117838No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC959201.9227689155298668No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA841181.6861913640173203No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG788461.5805112376341524No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA767361.5382151324238935No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC682201.3675072499733896No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG442890.8877972529180804No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT381740.7652187299983021No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA226240.45351046648194027No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG223050.44711593683166895No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA138290.27720987628088545No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128830.25824678835249454No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG125570.2517119398697721No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA116310.23314976289124148No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC107070.21462767700769686No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT102100.2046650399036691No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60480.1212354712377464No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC6350.024.4384673
TCTAACG6400.024.2475412
GTACGCA62650.023.5690633
GCTTATG23850.023.4634721
GAGTACG63250.023.419831
TACGCAG63450.023.0495534
TAACGCC7600.022.8940094
AGTACGC66000.022.5152572
TATTCGC1903.6468919E-722.2618589
CTGTGCG13600.022.1163569
TAGTACT13400.022.109024
CGCCTAC7100.021.8437947
CTAGTAC14000.021.8332823
CGCAGTG66350.021.8170176
ACGCCTA7400.021.5939836
TATTCTC10000.021.1498245
GGACCGT1354.080466E-420.8882966
GTACTAG10000.020.6912361
GAGTACT56450.020.6889912-13
TACCCCG2501.0741132E-820.6798295
Produced by FastQC (version 0.11.5)