FastQCFastQC Report
Mon 16 Jan 2017
ERR1146627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014900
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19600.022.0767771
TAGTACT20550.021.7357624
CTATTGA24600.021.2050749
CATGGGG94850.020.9188694
GGGCTAT28400.020.5199786
CTAGTAC21950.020.3501873
AGGGCTA29300.019.8971125
GTGATCG13300.019.7876268
CTAACGC7400.019.6974183
GCTATTG26950.019.0075028
GCATCGT19350.018.943978
CTGTGCG22350.018.9241719
GGCTATT30050.018.76727
TACATGG222700.018.6431712
ATTTAGG30300.018.6269861
GTACATG230050.018.604721
ACATGGG213500.018.5435773
CTAGGCA29000.018.158634
GTATAAG22600.018.1056771
TTTAGGG31800.017.7432612
Produced by FastQC (version 0.11.5)