FastQCFastQC Report
Mon 16 Jan 2017
ERR1146627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014900
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133360.33216269396498044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119480.2975914717676654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69330.17268176044235223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16750.027.5165331
CGACGAG9250.022.3555324-25
GAGTACT143550.021.98478712-13
TAGTACT25250.021.7897784
CTAGTAC25950.021.5644263
AGTACTT152150.020.77302612-13
CATGGGG90600.020.7096634
TAGGCAT26400.020.1173235
CTAACGC9600.020.083573
CTGTGCG27000.019.6692929
GTACTTT160450.019.50805514-15
CTAGGCA28200.019.3435964
GCATCGT21950.018.841798
TCTAACG10250.018.8113862
CTATTGA24150.018.48769
ATGGGGG61300.018.4778845
GGGCTAT24700.018.4569386
TAATACA19000.018.067484
TACTTTT177100.018.01914-15
TACTGTG42150.017.8400657
Produced by FastQC (version 0.11.5)