FastQCFastQC Report
Mon 16 Jan 2017
ERR1146626_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146626_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381960
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA581701.7200085157719192No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA482881.4278110917929248No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA182760.5403966930419047No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG80210.23717016168139185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG51850.020.3093514
GTACTAG10900.018.982141
TTTGCGT7950.018.922553
TAACGCC5100.018.4355724
CTAACGC4850.018.4165673
ATTGCGT4700.017.9985076
GGCGTCG7600.017.9321928
TTGCGTT8400.017.9088424
TACGGGC8750.017.7297534
TCTAACG5350.017.5740992
CTTTGCG9400.017.5039882
ATGGGGG35350.017.2879835
TGCGCGA4701.8189894E-1217.02328194
CGCCTAC5000.016.9183447
TACCTGG27600.016.692212
ATACGGG8900.016.3745173
GTACATG181100.016.124721
ACATGGG161200.016.0396353
CGGGCGT8500.016.0339646
TACATGG179450.015.8230542
Produced by FastQC (version 0.11.5)