##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146626_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3381960 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.349795976297766 31.0 31.0 34.0 28.0 34.0 2 31.499617677323208 33.0 31.0 34.0 28.0 34.0 3 31.548196016511135 33.0 31.0 34.0 28.0 34.0 4 35.00581112727531 37.0 35.0 37.0 32.0 37.0 5 35.02868543684727 37.0 35.0 37.0 32.0 37.0 6 35.123704301647564 37.0 35.0 37.0 32.0 37.0 7 35.033604477876736 37.0 35.0 37.0 32.0 37.0 8 35.058564264509336 37.0 35.0 37.0 32.0 37.0 9 36.68296431655017 39.0 37.0 39.0 32.0 39.0 10-11 36.678867136216866 39.0 37.0 39.0 32.0 39.0 12-13 36.67043297377852 39.0 37.0 39.0 32.0 39.0 14-15 37.97340403198145 40.0 38.0 41.0 33.0 41.0 16-17 37.98021591030054 40.0 38.0 41.0 33.0 41.0 18-19 38.03483926480502 40.0 38.0 41.0 32.0 41.0 20-21 38.115519550793024 40.0 38.0 41.0 33.0 41.0 22-23 38.114185117505826 40.0 38.0 41.0 32.0 41.0 24-25 38.10661022010905 40.0 38.0 41.0 32.0 41.0 26-27 38.1031446261931 40.0 38.0 41.0 32.0 41.0 28-29 38.05574371074762 40.0 38.0 41.0 32.0 41.0 30-31 38.03897902399792 40.0 38.0 41.0 32.0 41.0 32-33 38.01467861831601 40.0 38.0 41.0 32.0 41.0 34-35 37.96566162816828 40.0 38.0 41.0 32.0 41.0 36-37 37.83553501519829 40.0 38.0 41.0 32.0 41.0 38-39 37.75267773717016 40.0 38.0 41.0 31.0 41.0 40-41 37.69954316431891 40.0 38.0 41.0 31.0 41.0 42-43 37.596869714603365 40.0 37.5 41.0 31.0 41.0 44-45 37.54072239174916 40.0 37.0 41.0 31.0 41.0 46-47 37.42641574708158 40.0 37.0 41.0 31.0 41.0 48-49 37.29154011283398 40.0 37.0 41.0 30.0 41.0 50-51 37.150222208423514 40.0 36.5 41.0 30.0 41.0 52-53 36.98081364061077 40.0 36.0 41.0 30.0 41.0 54-55 36.88890096275533 39.0 36.0 41.0 30.0 41.0 56-57 36.73294790594803 39.0 35.0 41.0 30.0 41.0 58-59 36.56576201374351 39.0 35.0 41.0 29.5 41.0 60-61 36.29730141692983 39.0 35.0 40.5 29.0 41.0 62-63 35.998567398786506 38.0 35.0 40.0 28.5 41.0 64-65 35.631615098936706 37.5 34.0 40.0 28.0 41.0 66-67 35.3908483542088 37.0 34.0 40.0 28.0 41.0 68-69 35.184179883854334 37.0 34.0 39.5 28.0 41.0 70-71 34.861767288791114 36.0 34.0 39.0 28.0 41.0 72-73 34.42778817608724 35.5 34.0 39.0 27.5 40.5 74-75 34.05085468190043 35.0 33.0 37.5 26.5 40.0 76-77 33.71496617346155 35.0 33.0 37.0 26.0 39.0 78-79 33.408934907568394 35.0 33.0 37.0 26.0 39.0 80-81 33.18512282818247 35.0 33.0 36.0 26.0 39.0 82-83 32.93921971282924 35.0 33.0 36.0 26.0 37.0 84-85 32.742574867828125 35.0 33.0 35.5 26.0 37.0 86-87 32.43810349619747 35.0 32.0 35.0 25.0 37.0 88-89 32.19373617665496 35.0 32.0 35.0 25.0 36.0 90-91 32.03135711244367 35.0 32.0 35.0 25.0 36.0 92-93 31.939756531715336 35.0 32.0 35.0 25.0 36.0 94-95 31.904503601461876 35.0 32.0 35.0 25.0 36.0 96-97 31.79369596328756 35.0 32.0 35.0 24.0 35.0 98-99 31.624688198559415 35.0 32.0 35.0 24.0 35.0 100 30.271343540432177 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28963017040918615 1101 2 0.3821641995927685 1101 3 0.2834353794911628 1101 4 0.30868405086955875 1101 5 0.29903783014228225 1101 6 0.28893453600043273 1101 7 0.3547830333179718 1101 8 0.350515799406665 1101 9 0.4969528650385726 1101 10-11 0.4765575806266469 1101 12-13 0.502178840965918 1101 14-15 0.5713683498838407 1101 16-17 0.5773405680924668 1101 18-19 0.5412983430008609 1101 20-21 0.5689055695558309 1101 22-23 0.517952171096816 1101 24-25 0.6114589756473023 1101 26-27 0.5495951126425211 1101 28-29 0.5732672654671092 1101 30-31 0.6765926981525823 1101 32-33 0.6424590321178485 1101 34-35 0.5896260127551542 1101 36-37 0.5758530661292696 1101 38-39 0.6363196209608262 1101 40-41 0.621031396217937 1101 42-43 0.6485308155982068 1101 44-45 0.563413402319803 1101 46-47 0.5850402694557175 1101 48-49 0.5991005859660845 1101 50-51 0.5769018820893166 1101 52-53 0.559163350155309 1101 54-55 0.5021569051953705 1101 56-57 0.6095582714439658 1101 58-59 0.583924122470016 1101 60-61 0.558356596059852 1101 62-63 0.5475718832312211 1101 64-65 0.6247464100428033 1101 66-67 0.600678963314131 1101 68-69 0.570326802060535 1101 70-71 0.600342381467307 1101 72-73 0.5937777414188474 1101 74-75 0.6856107417226767 1101 76-77 0.5593852151234557 1101 78-79 0.5316166426593014 1101 80-81 0.47346505765386127 1101 82-83 0.5407535422129612 1101 84-85 0.5019752175183072 1101 86-87 0.558197136041457 1101 88-89 0.5529127799122548 1101 90-91 0.5419296666408897 1101 92-93 0.48522942837763594 1101 94-95 0.4542277851422405 1101 96-97 0.4085460760800075 1101 98-99 0.50223822231181 1101 100 0.5622023637140288 1102 1 0.12807784342079387 1102 2 0.2517418618393954 1102 3 0.21831475328636074 1102 4 0.23051192799856324 1102 5 0.19225422295561145 1102 6 0.24330269835972018 1102 7 0.23781441866257325 1102 8 0.31575742733887324 1102 9 0.38921042347406143 1102 10-11 0.34740158853765024 1102 12-13 0.38996726318684694 1102 14-15 0.44549734607019786 1102 16-17 0.38250609011060277 1102 18-19 0.36390939337930917 1102 20-21 0.4009155334222072 1102 22-23 0.43900814065445104 1102 24-25 0.5245046308507924 1102 26-27 0.42337153483006773 1102 28-29 0.43761264612185613 1102 30-31 0.3471417121971214 1102 32-33 0.4036057946957641 1102 34-35 0.40852856881232924 1102 36-37 0.43882319956496474 1102 38-39 0.37880958616239724 1102 40-41 0.3353968047448035 1102 42-43 0.38336971640181616 1102 44-45 0.34688832158244765 1102 46-47 0.3119902748799319 1102 48-49 0.2882838660308451 1102 50-51 0.40506941523962325 1102 52-53 0.3280663234598151 1102 54-55 0.318885185385291 1102 56-57 0.2874938150755142 1102 58-59 0.32941910298456634 1102 60-61 0.26285535683526007 1102 62-63 0.23305376217942353 1102 64-65 0.3205342981871411 1102 66-67 0.2756170020847577 1102 68-69 0.21885391896489637 1102 70-71 0.23489004363047883 1102 72-73 0.23884712965080723 1102 74-75 0.3267104388011077 1102 76-77 0.3387940590979923 1102 78-79 0.20133199652676836 1102 80-81 0.1827488108771007 1102 82-83 0.2274905011474786 1102 84-85 0.3039195901034901 1102 86-87 0.13202738313532336 1102 88-89 0.21567721041136423 1102 90-91 0.200829135067778 1102 92-93 0.20395548881173653 1102 94-95 0.18304914486785506 1102 96-97 0.19979639944669714 1102 98-99 0.24921818609474045 1102 100 0.1623450623029008 1103 1 0.258518582674089 1103 2 0.23255264921323615 1103 3 0.2632169449924575 1103 4 0.27145300370328584 1103 5 0.21043805381234648 1103 6 0.3310081965427827 1103 7 0.2867112503995628 1103 8 0.35855477506493116 1103 9 0.3566696847536619 1103 10-11 0.34274579360394597 1103 12-13 0.37825773630682136 1103 14-15 0.4682208973307169 1103 16-17 0.44339263113320726 1103 18-19 0.4181917248108462 1103 20-21 0.46424034202762243 1103 22-23 0.4631542589157931 1103 24-25 0.5264365014964838 1103 26-27 0.4504312885059676 1103 28-29 0.48768228599529806 1103 30-31 0.4941922758319848 1103 32-33 0.5416878568692738 1103 34-35 0.5448104826468523 1103 36-37 0.5368728228798574 1103 38-39 0.5159183372421197 1103 40-41 0.4164396442882392 1103 42-43 0.4938018905562487 1103 44-45 0.4825500408710184 1103 46-47 0.48067296945615823 1103 48-49 0.48765117264152025 1103 50-51 0.552674288044507 1103 52-53 0.5553508140907724 1103 54-55 0.4626589446934233 1103 56-57 0.5211050215966182 1103 58-59 0.48425286110094135 1103 60-61 0.545942096386014 1103 62-63 0.47235746517977617 1103 64-65 0.5328142180201425 1103 66-67 0.5773042248272233 1103 68-69 0.4741784504641515 1103 70-71 0.37118823502846254 1103 72-73 0.47370744538709175 1103 74-75 0.4658537884233098 1103 76-77 0.455722410205027 1103 78-79 0.3390208053627717 1103 80-81 0.25898899407719966 1103 82-83 0.3028918327103014 1103 84-85 0.3273139610701321 1103 86-87 0.3563996190379157 1103 88-89 0.3028521133152182 1103 90-91 0.3629681764646264 1103 92-93 0.3109072195382083 1103 94-95 0.25253813595443475 1103 96-97 0.3071908702102988 1103 98-99 0.39075101905447696 1103 100 0.4642799123240451 1104 1 0.18356859549386684 1104 2 0.18687530407260766 1104 3 0.15114149808317734 1104 4 0.1827632957571126 1104 5 0.07096232600277119 1104 6 0.15210770634656967 1104 7 0.15825965541893794 1104 8 0.24268598390405316 1104 9 0.26898659926784063 1104 10-11 0.3037074006053615 1104 12-13 0.3550920417566985 1104 14-15 0.41889734245740584 1104 16-17 0.3616844895545128 1104 18-19 0.32170391042757984 1104 20-21 0.36196236107396373 1104 22-23 0.37017663729420036 1104 24-25 0.41910974679913693 1104 26-27 0.31976158125914367 1104 28-29 0.3430078820688607 1104 30-31 0.35421872636982954 1104 32-33 0.3442951248301398 1104 34-35 0.34425310138387744 1104 36-37 0.3249707220692599 1104 38-39 0.32101861152725064 1104 40-41 0.2780970760091961 1104 42-43 0.3731457725511831 1104 44-45 0.20832875590576094 1104 46-47 0.27946325107702563 1104 48-49 0.2577591032728179 1104 50-51 0.3113069948002192 1104 52-53 0.36812364955724064 1104 54-55 0.3591647685816852 1104 56-57 0.29183758773611146 1104 58-59 0.28663501024935556 1104 60-61 0.33013988134606365 1104 62-63 0.32909381349983846 1104 64-65 0.3658643537326469 1104 66-67 0.3064862703840774 1104 68-69 0.20446752239432442 1104 70-71 0.23610924369729247 1104 72-73 0.37104337183080816 1104 74-75 0.29790867294335754 1104 76-77 0.2669771929418516 1104 78-79 0.2880772484615477 1104 80-81 0.22523907100048035 1104 82-83 0.16660990885600313 1104 84-85 0.13334012873326628 1104 86-87 0.23016053141862614 1104 88-89 0.1874629220997548 1104 90-91 0.24904294949366346 1104 92-93 0.17997834887164288 1104 94-95 0.14962103438866947 1104 96-97 0.20886455477141652 1104 98-99 0.19828246118681392 1104 100 0.3098199903461669 1105 1 0.12315332441892579 1105 2 0.12360787441982524 1105 3 0.15829483309867243 1105 4 0.053149354497222134 1105 5 0.06980658489965919 1105 6 0.151367062659169 1105 7 0.15674596893269666 1105 8 0.18419871915238417 1105 9 0.1789459694861364 1105 10-11 0.2550588388213555 1105 12-13 0.24536396231511048 1105 14-15 0.3526541192175543 1105 16-17 0.24867872160550064 1105 18-19 0.2674065635648972 1105 20-21 0.31694282376177796 1105 22-23 0.3282213431433192 1105 24-25 0.33438943990439185 1105 26-27 0.3461641355532521 1105 28-29 0.31621368731572375 1105 30-31 0.304663443053208 1105 32-33 0.33474516274358024 1105 34-35 0.2952994047683788 1105 36-37 0.2814294914136326 1105 38-39 0.2740936824715803 1105 40-41 0.3162779384761265 1105 42-43 0.2939405720013326 1105 44-45 0.2906260628527306 1105 46-47 0.2839255158967333 1105 48-49 0.23078425107432565 1105 50-51 0.3001987002518689 1105 52-53 0.24703883918082425 1105 54-55 0.2489092316059427 1105 56-57 0.26763571068710945 1105 58-59 0.24141485763917103 1105 60-61 0.26780314856836185 1105 62-63 0.21652193445116552 1105 64-65 0.3489133978227841 1105 66-67 0.18610633507603325 1105 68-69 0.22266279857795723 1105 70-71 0.2862212733448146 1105 72-73 0.2027816321099749 1105 74-75 0.2373030246896306 1105 76-77 0.27647363169631944 1105 78-79 0.25743617761130366 1105 80-81 0.19183286558570956 1105 82-83 0.16686106676784362 1105 84-85 0.23182053264910252 1105 86-87 0.13275078853926914 1105 88-89 0.17691772410813655 1105 90-91 0.11893738411685462 1105 92-93 0.19746065020903814 1105 94-95 0.2022805413700972 1105 96-97 0.17431368874965614 1105 98-99 0.22996814418794997 1105 100 0.2536884001887074 1106 1 0.18039864865696487 1106 2 0.20456285117278483 1106 3 0.18522693438949034 1106 4 0.17954583122806156 1106 5 0.1581421660987985 1106 6 0.16422370951121934 1106 7 0.206844920119579 1106 8 0.294270535083065 1106 9 0.3316982310316874 1106 10-11 0.26389961156072417 1106 12-13 0.338513534903754 1106 14-15 0.3333543419079916 1106 16-17 0.3736999115034294 1106 18-19 0.34764422190416866 1106 20-21 0.375916366216714 1106 22-23 0.3692647891672962 1106 24-25 0.370280465514945 1106 26-27 0.39829603732084706 1106 28-29 0.35340858477611903 1106 30-31 0.3642402958728965 1106 32-33 0.3649235381160878 1106 34-35 0.3345906244668271 1106 36-37 0.3920999867033572 1106 38-39 0.4011611066245493 1106 40-41 0.38338096775103025 1106 42-43 0.40053115124386807 1106 44-45 0.36295920375558666 1106 46-47 0.43005324561564606 1106 48-49 0.44770074221978007 1106 50-51 0.45613392515605256 1106 52-53 0.41741140724776926 1106 54-55 0.3196098820550688 1106 56-57 0.34130136255418364 1106 58-59 0.3183818072481017 1106 60-61 0.3723877101845616 1106 62-63 0.35282291375044394 1106 64-65 0.43225236757756846 1106 66-67 0.400154040040988 1106 68-69 0.4622689632265349 1106 70-71 0.37124111537633553 1106 72-73 0.4483659376803075 1106 74-75 0.4098503983548696 1106 76-77 0.3888828868343879 1106 78-79 0.39356148336431573 1106 80-81 0.3304509929751731 1106 82-83 0.3975443801939491 1106 84-85 0.3869877418137193 1106 86-87 0.2972903002192169 1106 88-89 0.33718367058029486 1106 90-91 0.34104419680156894 1106 92-93 0.45017384069047495 1106 94-95 0.3539883892552105 1106 96-97 0.42907061518040024 1106 98-99 0.38015781433968954 1106 100 0.47326887737972356 1107 1 0.051237707362556506 1107 2 0.1181870766271409 1107 3 0.09029723763977415 1107 4 0.05182293060324383 1107 5 0.06874512341561001 1107 6 0.12597390179730894 1107 7 0.11725429503856333 1107 8 0.09460757586499824 1107 9 0.19238631275111828 1107 10-11 0.16635828085458115 1107 12-13 0.19464378820286043 1107 14-15 0.23858625189217264 1107 16-17 0.2831932268612576 1107 18-19 0.2666066548200874 1107 20-21 0.33388305967379495 1107 22-23 0.30347442853366147 1107 24-25 0.3424469463770734 1107 26-27 0.2757480090094191 1107 28-29 0.27507512181171734 1107 30-31 0.3073158362184074 1107 32-33 0.3269390419920484 1107 34-35 0.2383205424535717 1107 36-37 0.3102094471788419 1107 38-39 0.25642888120476215 1107 40-41 0.22898431396105678 1107 42-43 0.30016904845507497 1107 44-45 0.2673441907492773 1107 46-47 0.27156457105012777 1107 48-49 0.3147908703816995 1107 50-51 0.30989452249602323 1107 52-53 0.2779638084472893 1107 54-55 0.23326781158761634 1107 56-57 0.26452001560094374 1107 58-59 0.2358016304313253 1107 60-61 0.3063087043684476 1107 62-63 0.2614831334319021 1107 64-65 0.2779274429796388 1107 66-67 0.2274076761925059 1107 68-69 0.2782611162257709 1107 70-71 0.27791279444436867 1107 72-73 0.34629184056071693 1107 74-75 0.26562978355791955 1107 76-77 0.268120310460084 1107 78-79 0.2185241064058019 1107 80-81 0.15006935910516717 1107 82-83 0.21148749808044442 1107 84-85 0.14951312789825977 1107 86-87 0.14038917415732044 1107 88-89 0.1614497426724526 1107 90-91 0.2304360542419559 1107 92-93 0.18814191687896908 1107 94-95 0.22385348652172254 1107 96-97 0.18537370990613766 1107 98-99 0.21289398054400266 1107 100 0.3135979674560332 1108 1 0.046141133744995955 1108 2 0.026691142492268227 1108 3 0.009184943406154389 1108 4 0.055541912163434404 1108 5 0.06074602219103298 1108 6 0.0018122534591000772 1108 7 0.010497671292398536 1108 8 0.0526753280217136 1108 9 0.0025780680774047937 1108 10-11 0.09870206642903412 1108 12-13 -0.02121860371293849 1108 14-15 -0.04978365384577188 1108 16-17 -0.01983556676141518 1108 18-19 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-0.4440536907169168 2215 4 -0.3411783686576513 2215 5 -0.3555493080267027 2215 6 -0.38931837585879947 2215 7 -0.4106411429765444 2215 8 -0.399399010441563 2215 9 -0.5606424552785612 2215 10-11 -0.5369585597886442 2215 12-13 -0.592642592833009 2215 14-15 -0.7095415068943183 2215 16-17 -0.7031292417146631 2215 18-19 -0.7076640892598007 2215 20-21 -0.683916484909048 2215 22-23 -0.6443276413824677 2215 24-25 -0.5918277274642634 2215 26-27 -0.7005468589265291 2215 28-29 -0.6710605413338797 2215 30-31 -0.6753719821474888 2215 32-33 -0.6750620044058309 2215 34-35 -0.702765443166534 2215 36-37 -0.6236558160706664 2215 38-39 -0.7540999947944513 2215 40-41 -0.7601128398300929 2215 42-43 -0.7932372272289001 2215 44-45 -0.7487365447491001 2215 46-47 -0.7039665813755036 2215 48-49 -0.6873244588858256 2215 50-51 -0.7606795424630661 2215 52-53 -0.7550690508860427 2215 54-55 -0.7636544302357962 2215 56-57 -0.8170474745903178 2215 58-59 -0.7376612377736507 2215 60-61 -0.7632382818309011 2215 62-63 -0.833227842833594 2215 64-65 -0.7427845262759121 2215 66-67 -0.6864097211119855 2215 68-69 -0.6914094662459789 2215 70-71 -0.6217820885908267 2215 72-73 -0.5441238438029501 2215 74-75 -0.5194465682124374 2215 76-77 -0.5871057942331106 2215 78-79 -0.5816870523364486 2215 80-81 -0.5585903416856937 2215 82-83 -0.5862770874500569 2215 84-85 -0.5724139189816242 2215 86-87 -0.5789716264062932 2215 88-89 -0.5629607519005546 2215 90-91 -0.6308246409236133 2215 92-93 -0.6562603818996067 2215 94-95 -0.5934758878187196 2215 96-97 -0.6019098523830486 2215 98-99 -0.7011825930996238 2215 100 -0.6747535206938586 2216 1 -0.2829036158136198 2216 2 -0.32460278779272755 2216 3 -0.31940104409320114 2216 4 -0.2914207370627935 2216 5 -0.29727475611500864 2216 6 -0.33235087198209357 2216 7 -0.38724015180060434 2216 8 -0.3567313199765749 2216 9 -0.4313784274417145 2216 10-11 -0.5261433896605467 2216 12-13 -0.5174303105602363 2216 14-15 -0.7044041544636386 2216 16-17 -0.6350577718423978 2216 18-19 -0.6716051168233008 2216 20-21 -0.5796153435220504 2216 22-23 -0.6379914424817343 2216 24-25 -0.6603595190372502 2216 26-27 -0.7885483956574433 2216 28-29 -0.7580292801759185 2216 30-31 -0.7329493470415045 2216 32-33 -0.745743669097763 2216 34-35 -0.7392446396846566 2216 36-37 -0.841072883498228 2216 38-39 -0.7308158344391131 2216 40-41 -0.7165746796597432 2216 42-43 -0.7706521399261561 2216 44-45 -0.7679930129446078 2216 46-47 -0.8038958421479876 2216 48-49 -0.7586179739743599 2216 50-51 -0.8158434285630918 2216 52-53 -0.8646207067988669 2216 54-55 -0.8215663467211343 2216 56-57 -0.7902238467582308 2216 58-59 -0.8510002584926468 2216 60-61 -0.9499192324131656 2216 62-63 -0.9705497352551902 2216 64-65 -0.9238197475904855 2216 66-67 -0.8045779213231157 2216 68-69 -0.9058922250448518 2216 70-71 -0.9323726913830868 2216 72-73 -0.9110765332629143 2216 74-75 -0.8693029711976408 2216 76-77 -0.9005660637449182 2216 78-79 -0.7889109277816644 2216 80-81 -0.7226575785722034 2216 82-83 -0.8012106602667686 2216 84-85 -0.8046930449155347 2216 86-87 -0.872414503059094 2216 88-89 -0.880285894502876 2216 90-91 -0.9176972953291553 2216 92-93 -0.7794745264380865 2216 94-95 -0.7745251122966614 2216 96-97 -0.8130711486729325 2216 98-99 -0.8706087010934667 2216 100 -0.8954971661771651 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1033.0 1 246.0 2 195.0 3 171.0 4 148.0 5 179.0 6 293.0 7 871.0 8 2218.0 9 2859.0 10 3100.0 11 4240.0 12 5329.0 13 6782.0 14 8377.0 15 9053.0 16 9586.0 17 10429.0 18 11239.0 19 12717.0 20 13869.0 21 15409.0 22 17142.0 23 19773.0 24 23998.0 25 29093.0 26 32168.0 27 32395.0 28 35975.0 29 43405.0 30 51849.0 31 61064.0 32 77463.0 33 104760.0 34 152144.0 35 234548.0 36 412869.0 37 773295.0 38 888840.0 39 272185.0 40 651.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.43707020677429 18.368506025059155 12.788219503641079 23.406204264525478 2 13.878640302733537 21.14776603833875 40.64833854556536 24.32525511336235 3 19.937195803268754 26.869330341079106 30.319063310796736 22.874410544855404 4 11.978957984028806 16.583267590596254 40.48957575664317 30.94819866873177 5 13.150223375919316 39.45706201999775 34.82557384248306 12.567140761599878 6 28.538105566451133 38.08322287319472 19.195057067865584 14.183614492488564 7 26.030753477966222 34.3384243873423 22.059553790472698 17.57126834421878 8 23.386298220765685 34.86774857402605 21.20985684328403 20.53609636192423 9 25.64019712712806 18.215647372649276 21.025818564333363 35.118336935889296 10-11 23.531399617411463 25.66204698923476 29.160295246258265 21.646258147095512 12-13 27.44863215094384 23.304896117739393 27.393375235877492 21.85309649543927 14-15 24.3060041504667 24.948163553126182 26.770377042645997 23.97545525376112 16-17 21.669768651004496 27.912769211625466 28.473700898872643 21.943761238497395 18-19 21.17874671180781 30.131654931833314 27.98168510796456 20.707913248394316 20-21 22.408711370604006 28.769215018539267 25.797842514890153 23.024231095966574 22-23 21.996447375078894 28.021691547764995 24.865267454422625 25.116593622733486 24-25 22.83827539044352 27.990031846247902 26.30437342906738 22.867319334241195 26-27 24.297069803760973 26.424568211118288 26.73630600990708 22.542055975213657 28-29 22.357120430669454 26.85286318426584 28.395210601494924 22.394805783569776 30-31 22.31922610883891 29.221162301615074 27.01223672414051 21.447374865405504 32-33 22.039832605869446 28.88580094469193 25.772345284575053 23.302021164863568 34-35 22.85395847788447 27.21664684568146 26.325701329197194 23.603693347236877 36-37 24.071964352540274 27.02196832563451 27.153931284262388 21.75213603756283 38-39 25.040697085095786 25.550370642755727 27.721947274689974 21.686984997458513 40-41 22.61647717688337 26.658753256069563 29.136725031769057 21.58804453527801 42-43 21.951245859564967 28.503624878361773 27.3165275275299 22.228601734543364 44-45 22.029114480545335 29.23846797760798 25.93340902697759 22.799008514869097 46-47 22.01070583458515 27.63843067901081 25.174277586996745 25.176585899407293 48-49 23.073144975572564 27.293481114353895 26.147920715831706 23.48545319424183 50-51 24.42447096580676 27.658940364521328 26.218843314309716 21.6977453553622 52-53 22.11345312436644 27.717473822163857 27.901182608664772 22.267890444804937 54-55 21.31035396115239 27.391403747736927 28.284397105924953 23.013845185185733 56-57 22.78249820976772 28.8376490546878 25.82156456312339 22.558288172421083 58-59 21.97447525664446 27.514411277557237 26.84709424365803 23.66401922214027 60-61 22.355768981749573 26.408191359021334 27.482738213541065 23.753301445688027 62-63 25.006689133473074 25.898030967728186 26.36508639341458 22.73019350538416 64-65 23.56464948876224 26.579509347903624 27.87766403783855 21.978177125495588 66-67 22.148231168009698 26.692692127081163 28.39426766985749 22.76480903505164 68-69 21.585033848499847 28.698886870949487 27.36180305121909 22.354276229331578 70-71 21.836976711867955 28.062482527278164 25.95948089681145 24.14105986404243 72-73 21.90535161459176 27.30590690729844 25.624889284058373 25.163852194051433 74-75 22.621812354481396 27.544714503820412 26.500917288897757 23.33255585280044 76-77 23.689015235433903 25.970726691437733 27.176114760864795 23.164143312263572 78-79 22.30330514112508 26.054099735147663 28.505097612036835 23.13749751169043 80-81 21.750071260468186 28.47688186094752 27.484799986739482 22.288246891844814 82-83 21.821880815590987 27.76912782748539 26.62849867877765 23.780492678145972 84-85 22.47388146732638 26.439701235243206 26.53626935950396 24.550147937926454 86-87 23.258724929899692 26.52398520490216 27.43397577334547 22.783314091852677 88-89 24.336077242043945 24.826283984562128 27.880649072560036 22.956989700833887 90-91 22.393319567209158 25.85266872936543 29.01544389830827 22.738567805117142 92-93 21.745092854015134 27.622288160060542 27.77169157488158 22.86092741104274 94-95 21.303726942456954 28.096558664012843 26.879120391266063 23.720594002264132 96-97 21.49799198629469 26.687174712360306 25.943294385508146 25.871538915836854 98-99 22.425556848257877 26.54170292062747 26.43509814580508 24.59764208530957 100 22.039844586431308 28.28836994784374 27.063664075254884 22.60812139047007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1808.0 1 1192.0 2 603.0 3 602.0 4 550.5 5 471.5 6 398.5 7 394.0 8 419.0 9 456.0 10 432.0 11 369.0 12 397.5 13 458.0 14 483.5 15 560.0 16 659.0 17 748.5 18 927.5 19 1202.5 20 1721.5 21 2562.0 22 3707.5 23 5506.5 24 8010.5 25 11002.5 26 14959.0 27 19906.0 28 24390.5 29 29466.0 30 35532.0 31 41020.0 32 47599.0 33 54394.0 34 60816.0 35 67659.0 36 74359.0 37 81757.0 38 85780.0 39 87422.5 40 89272.0 41 89913.5 42 91862.5 43 96853.5 44 103808.5 45 112805.0 46 128194.0 47 158267.5 48 291875.5 49 292564.5 50 164859.5 51 137140.5 52 117428.5 53 106985.5 54 98500.0 55 87946.0 56 78413.0 57 69021.0 58 59651.5 59 52327.5 60 43860.0 61 34547.0 62 26788.5 63 20519.0 64 15182.0 65 10882.5 66 8011.0 67 6021.5 68 4498.0 69 3283.5 70 2562.5 71 2014.0 72 1475.0 73 1194.5 74 961.5 75 739.0 76 552.0 77 400.0 78 258.0 79 158.0 80 105.5 81 75.5 82 67.0 83 49.0 84 41.0 85 31.5 86 24.0 87 21.0 88 17.0 89 14.5 90 13.0 91 11.5 92 10.0 93 11.5 94 10.0 95 11.0 96 12.5 97 8.5 98 6.5 99 8.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09328909862919726 2 0.06179848371949993 3 0.07593229961324202 4 0.07764728145808938 5 0.07921442004044991 6 0.08613348472483412 7 0.08367928656755255 8 0.0847437580574578 9 0.07140829578114466 10-11 0.07721853599687754 12-13 0.06778613585021703 14-15 0.07631669209570782 16-17 0.07899265514671965 18-19 0.07786904635181965 20-21 0.07028468698624467 22-23 0.07353723876095519 24-25 0.07681935918816307 26-27 0.0696933139362973 28-29 0.07874132160049202 30-31 0.08363493358880648 32-33 0.08483246401494991 34-35 0.08768583898094595 36-37 0.08815893742090386 38-39 0.08966693869826964 40-41 0.08533513110740518 42-43 0.09130799891187358 44-45 0.08836591798838543 46-47 0.08480289536245254 48-49 0.09393960898413937 50-51 0.091381920543117 52-53 0.09589114004896568 54-55 0.08833634933588806 56-57 0.09487102153780648 58-59 0.09923239778116832 60-61 0.09836012253249594 62-63 0.09893671125619465 64-65 0.10038557522856568 66-67 0.1027067144496091 68-69 0.09982377083111568 70-71 0.10288412636459332 72-73 0.09998639841985121 74-75 0.10425906870572095 76-77 0.10116914451974596 78-79 0.10829518977161173 80-81 0.10375640161326567 82-83 0.10789601296289726 84-85 0.10396338218074727 86-87 0.10078475203728016 88-89 0.10010467302984068 90-91 0.09983855515736437 92-93 0.09427964848785911 94-95 0.09417615820411832 96-97 0.09235768607553016 98-99 0.10625495274929331 100 0.24400052040828396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3381960.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.5429124856268 #Duplication Level Percentage of deduplicated Percentage of total 1 73.70009121496322 43.88318081395268 2 14.565404566127294 17.345332187973327 3 5.527315925733547 9.873374653390917 4 2.4620067211376986 5.863802029429078 5 1.3362269879874706 3.9781423303335326 6 0.7993314813321235 2.8556714663979035 7 0.4615487233563195 1.9237368669860673 8 0.3052953437709212 1.4542539149137048 9 0.20669608090049546 1.1076557990562215 >10 0.6286324022333896 5.39649204253573 >50 0.005818585521407764 0.22559029863395857 >100 9.941828055354408E-4 0.09381615941525269 >500 7.086490339358184E-5 0.022363364527652137 >1k 2.834596135720813E-4 0.37498720859560325 >5k 7.086490339302032E-5 0.33844631485825744 >10k+ 2.1259471017906094E-4 5.26315454900011 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 58170 1.7200085157719192 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 48288 1.4278110917929248 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 18276 0.5403966930419047 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 8021 0.23717016168139185 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.956865249736839E-5 5 0.0 2.956865249736839E-5 0.0 0.0 2.956865249736839E-5 6 0.0 2.956865249736839E-5 0.0 0.0 5.913730499473678E-5 7 0.0 2.956865249736839E-5 0.0 0.0 5.913730499473678E-5 8 0.0 2.956865249736839E-5 0.0 0.0 5.913730499473678E-5 9 0.0 2.956865249736839E-5 0.0 0.0 8.870595749210517E-5 10-11 0.0 2.956865249736839E-5 0.0 0.0 8.870595749210517E-5 12-13 0.0 7.392163124342098E-5 0.0 0.0 1.1827460998947356E-4 14-15 0.0 1.3305893623815777E-4 0.0 0.0 1.4784326248684196E-4 16-17 0.0 1.4784326248684196E-4 0.0 0.0 1.4784326248684196E-4 18-19 0.0 1.4784326248684196E-4 0.0 5.913730499473678E-5 2.3654921997894713E-4 20-21 0.0 1.4784326248684196E-4 0.0 2.6611787247631555E-4 2.3654921997894713E-4 22-23 0.0 1.4784326248684196E-4 0.0 5.470200712013152E-4 3.252551774710523E-4 24-25 0.0 1.9219624123289456E-4 0.0 0.001123608794899999 4.139611349631575E-4 26-27 0.0 2.0698056748157874E-4 0.0 0.0020106683698210507 4.5831411370921005E-4 28-29 0.0 2.2176489373026292E-4 0.0 0.0051449455345420995 4.7309843995789426E-4 30-31 0.0 2.3654921997894713E-4 0.0 0.01339459958130788 4.7309843995789426E-4 32-33 0.0 2.3654921997894713E-4 0.0 0.0323628901583697 5.32235744952631E-4 34-35 0.0 2.809021987249997E-4 0.0 0.07012205939750914 5.618043974499994E-4 36-37 0.0 3.1047085122236806E-4 0.0 0.1282392458810867 5.765887236986836E-4 38-39 0.0 3.252551774710523E-4 0.0 0.20071201315213663 6.06157376196052E-4 40-41 2.956865249736839E-5 3.252551774710523E-4 0.0 0.282528474612355 6.505103549421045E-4 42-43 2.956865249736839E-5 4.4352978746052584E-4 0.0 0.3633248175614141 6.80079007439473E-4 44-45 2.956865249736839E-5 4.4352978746052584E-4 0.0 0.4616258027889153 6.80079007439473E-4 46-47 2.956865249736839E-5 4.4352978746052584E-4 0.0 0.5911217164011402 6.80079007439473E-4 48-49 2.956865249736839E-5 4.7309843995789426E-4 0.0 0.7423653739251794 7.687849649315781E-4 50-51 2.956865249736839E-5 5.026670924552627E-4 0.0 0.8880205561272161 7.983536174289465E-4 52-53 2.956865249736839E-5 5.026670924552627E-4 0.0 1.0321381683993898 8.870595749210517E-4 54-55 2.956865249736839E-5 5.322357449526311E-4 0.0 1.1887041833729555 9.166282274184201E-4 56-57 2.956865249736839E-5 5.618043974499994E-4 0.0 1.3715862990691787 0.0010201185111592093 58-59 2.956865249736839E-5 5.618043974499994E-4 0.0 1.613886621958864 0.001064471489905262 60-61 2.956865249736839E-5 6.06157376196052E-4 0.0 1.9156199363682598 0.0011088244686513146 62-63 2.956865249736839E-5 6.209417024447362E-4 0.0 2.1885681675714674 0.001212314752392104 64-65 2.956865249736839E-5 6.209417024447362E-4 0.0 2.4378614767767806 0.001212314752392104 66-67 2.956865249736839E-5 6.505103549421045E-4 0.0 2.7156589669895563 0.001212314752392104 68-69 2.956865249736839E-5 6.505103549421045E-4 0.0 3.020659617499911 0.001212314752392104 70-71 2.956865249736839E-5 6.948633336881572E-4 0.0 3.3768583898094597 0.0012566677311381565 72-73 2.956865249736839E-5 7.392163124342098E-4 0.0 3.762965854120096 0.0013158050361328933 74-75 2.956865249736839E-5 7.392163124342098E-4 0.0 4.129809341328697 0.0013305893623815775 76-77 2.956865249736839E-5 7.983536174289465E-4 0.0 4.499934948964506 0.001360158014878946 78-79 2.956865249736839E-5 7.983536174289465E-4 0.0 4.910259139670487 0.0014045109936249986 80-81 2.956865249736839E-5 7.983536174289465E-4 0.0 5.362349052029001 0.0014192953198736828 82-83 2.956865249736839E-5 7.983536174289465E-4 0.0 5.838079102059162 0.0014488639723710512 84-85 2.956865249736839E-5 7.983536174289465E-4 0.0 6.415880140510236 0.0014488639723710512 86-87 2.956865249736839E-5 7.983536174289465E-4 0.0 7.027729482312032 0.0014488639723710512 88 2.956865249736839E-5 7.983536174289465E-4 0.0 7.439265987770406 0.0014784326248684196 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 5185 0.0 20.309351 4 GTACTAG 1090 0.0 18.98214 1 TTTGCGT 795 0.0 18.92255 3 TAACGCC 510 0.0 18.435572 4 CTAACGC 485 0.0 18.416567 3 ATTGCGT 470 0.0 17.998507 6 GGCGTCG 760 0.0 17.932192 8 TTGCGTT 840 0.0 17.908842 4 TACGGGC 875 0.0 17.729753 4 TCTAACG 535 0.0 17.574099 2 CTTTGCG 940 0.0 17.503988 2 ATGGGGG 3535 0.0 17.287983 5 TGCGCGA 470 1.8189894E-12 17.023281 94 CGCCTAC 500 0.0 16.918344 7 TACCTGG 2760 0.0 16.69221 2 ATACGGG 890 0.0 16.374517 3 GTACATG 18110 0.0 16.12472 1 ACATGGG 16120 0.0 16.039635 3 CGGGCGT 850 0.0 16.033964 6 TACATGG 17945 0.0 15.823054 2 >>END_MODULE