FastQCFastQC Report
Mon 16 Jan 2017
ERR1146626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381960
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA569371.683550367242664No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA473291.3994547540479485No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG114730.33924115010230754No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG109860.32484121633608914No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG83480.24683911104803133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79890.23622396480147606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76370.2258157991224024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.12545979254633408No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36520.10798471892038936No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT94350.021.26969312-13
TAACGCC4650.021.234124
TTTGCGT6850.019.9056033
GTACTAG11050.019.574141
TTGCGTT7100.019.2047024
AGTACTT101450.019.13256812-13
GTACTTT104400.019.10963614-15
CTAACGC4500.018.8073643
TATTCGC1550.00103425118.1926029
TGATCGC6600.017.8021059
CATGGGG52000.017.7223244
ACTGCGC9100.017.0430688
ACTTTTT119050.016.81726816-17
TATACTC44400.016.6258774
GTCCTAC10200.016.5954691
TACCTGG28250.016.4777362
CGCCTAC4951.8189894E-1216.1405757
TACTTTT120950.016.04794514-15
CTTTGCG9250.015.7579862
TCTAACG5400.015.6732662
Produced by FastQC (version 0.11.5)