FastQCFastQC Report
Mon 16 Jan 2017
ERR1146625_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146625_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3729560
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32650.034.4229471
GTATCAA51650.033.2319531
CAACGCA50750.031.308095
ATCAACG51900.031.0680733
AACGCAG59700.027.7866656
TATCAAC62450.027.0239982
TCAACGC61900.026.200894
GTACATG104250.024.4036371
ACGCAGA66100.024.1003117
TACATGG105500.023.304342
CGCAGAG69250.023.2073148
ACATGGG104250.022.9524633
GTACTAG15800.022.3222261
CATGGGG82600.021.114534
TAACGCC6650.019.7935624
CTAACGC7000.019.475453
TCTAACG6850.019.2156472
GCAGAGT82850.018.5467579
GTATATA24950.018.4709341
GTATAAG21250.017.7037121
Produced by FastQC (version 0.11.5)