##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146625_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3729560
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.988243385278693	31.0	31.0	34.0	27.0	34.0
2	31.098531998412682	31.0	31.0	34.0	27.0	34.0
3	31.291463336157616	31.0	31.0	34.0	28.0	34.0
4	34.79644113514731	37.0	35.0	37.0	32.0	37.0
5	34.786051973959395	37.0	35.0	37.0	32.0	37.0
6	34.87437794270638	37.0	35.0	37.0	32.0	37.0
7	34.71695240189191	37.0	35.0	37.0	31.0	37.0
8	34.72529976726477	37.0	35.0	37.0	31.0	37.0
9	36.28523391499265	39.0	37.0	39.0	31.0	39.0
10-11	36.31860621628289	39.0	37.0	39.0	31.5	39.0
12-13	36.28692285417047	39.0	37.0	39.0	31.0	39.0
14-15	37.580448229817996	40.0	38.0	41.0	31.5	41.0
16-17	37.5820799236371	40.0	38.0	41.0	31.0	41.0
18-19	37.57770769206019	40.0	38.0	41.0	31.0	41.0
20-21	37.70092356739133	40.0	38.0	41.0	31.0	41.0
22-23	37.69956442583039	40.0	38.0	41.0	31.0	41.0
24-25	37.71771294737181	40.0	38.0	41.0	31.0	41.0
26-27	37.79315629725758	40.0	38.0	41.0	31.0	41.0
28-29	37.7769413818252	40.0	38.0	41.0	31.0	41.0
30-31	37.74273359323888	40.0	38.0	41.0	31.0	41.0
32-33	37.71981292699407	40.0	38.0	41.0	31.0	41.0
34-35	37.66699892212486	40.0	38.0	41.0	30.5	41.0
36-37	37.595679249026695	40.0	37.5	41.0	30.0	41.0
38-39	37.53673824794346	40.0	37.0	41.0	30.0	41.0
40-41	37.491948379969756	40.0	37.0	41.0	30.0	41.0
42-43	37.318629409367325	40.0	37.0	41.0	30.0	41.0
44-45	37.176364638187884	40.0	37.0	41.0	30.0	41.0
46-47	37.06337878999132	40.0	36.0	41.0	30.0	41.0
48-49	36.96295675629297	40.0	36.0	41.0	30.0	41.0
50-51	36.83904334559573	40.0	36.0	41.0	30.0	41.0
52-53	36.72365721425584	39.0	35.0	41.0	29.5	41.0
54-55	36.6173599566705	39.0	35.0	41.0	29.0	41.0
56-57	36.443548434667896	39.0	35.0	41.0	29.0	41.0
58-59	36.26407981102328	39.0	35.0	40.0	29.0	41.0
60-61	36.05845702978367	38.5	35.0	40.0	29.0	41.0
62-63	35.842304454144724	38.0	35.0	40.0	28.0	41.0
64-65	35.56360053196624	37.5	34.0	40.0	28.0	41.0
66-67	35.34004105041882	37.0	34.0	40.0	28.0	41.0
68-69	35.09418751809865	36.5	34.0	39.5	28.0	41.0
70-71	34.81204860626991	36.0	34.0	39.0	28.0	41.0
72-73	34.47756102596553	35.5	34.0	39.0	27.5	40.5
74-75	34.151979321957555	35.0	33.5	38.5	27.0	40.0
76-77	33.87832116925321	35.0	33.0	37.0	27.0	39.5
78-79	33.56258687351859	35.0	33.0	37.0	26.0	39.0
80-81	33.31482105127682	35.0	33.0	36.5	26.0	39.0
82-83	33.03705490728129	35.0	33.0	36.0	26.0	37.5
84-85	32.81874684949431	35.0	33.0	36.0	26.0	37.0
86-87	32.602836527633286	35.0	33.0	35.0	26.0	37.0
88-89	32.416335975289314	35.0	33.0	35.0	25.5	36.0
90-91	32.266522056221106	35.0	32.5	35.0	25.0	36.0
92-93	32.11251836677785	35.0	32.0	35.0	25.0	36.0
94-95	31.98713521166036	35.0	32.0	35.0	25.0	36.0
96-97	31.89130125269469	35.0	32.0	35.0	25.0	35.0
98-99	31.783122673988352	35.0	32.0	35.0	24.5	35.0
100	30.592169049432105	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31631461965096364
1101	2	0.32193148603374055
1101	3	0.35014362182932857
1101	4	0.4303262949666262
1101	5	0.31776787238096915
1101	6	0.39205932639607965
1101	7	0.4547690369748736
1101	8	0.42821723736199147
1101	9	0.588904153231141
1101	10-11	0.5804074674027859
1101	12-13	0.5698401496638965
1101	14-15	0.713926817696823
1101	16-17	0.6947051344966368
1101	18-19	0.6489655606740641
1101	20-21	0.677442431645261
1101	22-23	0.7643289738827832
1101	24-25	0.7341374352760184
1101	26-27	0.7028439719253399
1101	28-29	0.6378577043245244
1101	30-31	0.6915897623038632
1101	32-33	0.7459866764003422
1101	34-35	0.6866534836104137
1101	36-37	0.6542343457042747
1101	38-39	0.653362982860358
1101	40-41	0.6611404671933414
1101	42-43	0.6608862626736212
1101	44-45	0.5560601424080858
1101	46-47	0.481525752019067
1101	48-49	0.49134837032553236
1101	50-51	0.547674382100027
1101	52-53	0.5218676320260869
1101	54-55	0.43071207326840266
1101	56-57	0.5290793800447702
1101	58-59	0.5402486408739051
1101	60-61	0.5304828659683452
1101	62-63	0.5609995699031529
1101	64-65	0.5865021234266621
1101	66-67	0.48657609821798786
1101	68-69	0.45447886127871584
1101	70-71	0.48595911939542447
1101	72-73	0.4721987580623832
1101	74-75	0.40685136055863325
1101	76-77	0.40650510626290526
1101	78-79	0.40709751311170095
1101	80-81	0.43624615262585564
1101	82-83	0.4620151770827192
1101	84-85	0.36854136183488606
1101	86-87	0.3862090080711198
1101	88-89	0.35845211333656835
1101	90-91	0.34360783604681444
1101	92-93	0.33885526091371787
1101	94-95	0.43727722529482094
1101	96-97	0.42249415409975555
1101	98-99	0.43996732357006607
1101	100	0.3649473909096912
1102	1	0.34138514875586523
1102	2	0.4001610692759492
1102	3	0.3970604147102641
1102	4	0.4234339219593082
1102	5	0.44972288646543035
1102	6	0.48992372929588157
1102	7	0.49303952610755175
1102	8	0.4873829605529565
1102	9	0.6736152795564436
1102	10-11	0.6783268845385066
1102	12-13	0.7459607010987881
1102	14-15	0.7886470247693964
1102	16-17	0.7181076428428526
1102	18-19	0.6978490396679291
1102	20-21	0.6890599701914439
1102	22-23	0.6903090962858585
1102	24-25	0.6954013932693002
1102	26-27	0.5967520570217459
1102	28-29	0.6290107044490796
1102	30-31	0.6606683245358482
1102	32-33	0.6966719968738957
1102	34-35	0.6485575136935111
1102	36-37	0.650646108676149
1102	38-39	0.6279428133326235
1102	40-41	0.6463467255258237
1102	42-43	0.7070541783945288
1102	44-45	0.6769207289549968
1102	46-47	0.5827313230716911
1102	48-49	0.6117970006419498
1102	50-51	0.6959771880668484
1102	52-53	0.6400269089001682
1102	54-55	0.5390031788549194
1102	56-57	0.6177909520766534
1102	58-59	0.6258540155453218
1102	60-61	0.6690813199537473
1102	62-63	0.578003901249879
1102	64-65	0.6374225467422789
1102	66-67	0.6000984692433917
1102	68-69	0.5155048928966153
1102	70-71	0.5123455820328857
1102	72-73	0.600243275589861
1102	74-75	0.5831421395940026
1102	76-77	0.6092971729188008
1102	78-79	0.5228475820202902
1102	80-81	0.5058460742660031
1102	82-83	0.5162438532211553
1102	84-85	0.5295802449957847
1102	86-87	0.47166209836620254
1102	88-89	0.5656419593669995
1102	90-91	0.5115804936802704
1102	92-93	0.4931430642479029
1102	94-95	0.5473920731923982
1102	96-97	0.5519636820970355
1102	98-99	0.5685206402488276
1102	100	0.6336055768007931
1103	1	0.25131760699833094
1103	2	0.24081716660621666
1103	3	0.285276877918907
1103	4	0.25566408094319826
1103	5	0.2705855651812712
1103	6	0.31220955400810624
1103	7	0.29612968867951395
1103	8	0.28426144350613214
1103	9	0.41265046918675097
1103	10-11	0.38167543039508445
1103	12-13	0.4076829955915784
1103	14-15	0.4645194817088978
1103	16-17	0.4531744699512714
1103	18-19	0.4078480520129588
1103	20-21	0.3315749022931769
1103	22-23	0.4195571085341072
1103	24-25	0.47392300670860976
1103	26-27	0.45991130710268635
1103	28-29	0.41411774636311094
1103	30-31	0.45937699037877167
1103	32-33	0.5090973665771088
1103	34-35	0.422667308627112
1103	36-37	0.4864634656740918
1103	38-39	0.4859563700231888
1103	40-41	0.3575603667218701
1103	42-43	0.4648686001126521
1103	44-45	0.3697568466733685
1103	46-47	0.3982572592318263
1103	48-49	0.3370536893127749
1103	50-51	0.363002497631868
1103	52-53	0.2432866756707739
1103	54-55	0.2610904179022455
1103	56-57	0.3261833497041309
1103	58-59	0.3074628912127366
1103	60-61	0.28618220874434286
1103	62-63	0.2587455301598922
1103	64-65	0.18102339395029077
1103	66-67	0.21379084976543794
1103	68-69	0.20530323001084128
1103	70-71	0.2437620407591723
1103	72-73	0.23406766789916134
1103	74-75	0.30520081464881343
1103	76-77	0.2204872378348668
1103	78-79	0.2298541316339069
1103	80-81	0.20999721338154842
1103	82-83	0.21759253508628262
1103	84-85	0.28305090741345396
1103	86-87	0.2396190445194506
1103	88-89	0.1907335152026306
1103	90-91	0.2683608988692967
1103	92-93	0.21867610954633676
1103	94-95	0.21873395133422946
1103	96-97	0.25136309119591616
1103	98-99	0.19563590002350395
1103	100	0.3077669956577971
1104	1	0.2157145779158931
1104	2	0.18539652872055257
1104	3	0.17382236245497396
1104	4	0.16011200492686584
1104	5	0.2146113610306415
1104	6	0.24532029699200564
1104	7	0.2374364288249069
1104	8	0.2469319055720831
1104	9	0.3115114156051959
1104	10-11	0.35120547993355444
1104	12-13	0.3561038346105576
1104	14-15	0.43882113067403594
1104	16-17	0.4062109533750231
1104	18-19	0.43008269914567876
1104	20-21	0.4744645118480406
1104	22-23	0.4456431252407569
1104	24-25	0.4895258622725791
1104	26-27	0.43167736441266413
1104	28-29	0.39177465018192237
1104	30-31	0.3930096113446879
1104	32-33	0.49633878569162704
1104	34-35	0.423917488097068
1104	36-37	0.3940579991225803
1104	38-39	0.4759554632833414
1104	40-41	0.4041930343473368
1104	42-43	0.39522230377462364
1104	44-45	0.4195197241514421
1104	46-47	0.37464070671175165
1104	48-49	0.342050122132612
1104	50-51	0.3214965603903295
1104	52-53	0.2669235425492076
1104	54-55	0.2794650724391232
1104	56-57	0.28792342473817456
1104	58-59	0.3644108620146653
1104	60-61	0.25608035022390396
1104	62-63	0.3026519474127909
1104	64-65	0.371111722261638
1104	66-67	0.26624575003832973
1104	68-69	0.26149517868999794
1104	70-71	0.23501401148999435
1104	72-73	0.3542542362358674
1104	74-75	0.32177436854952646
1104	76-77	0.27708108281705535
1104	78-79	0.2961690319089243
1104	80-81	0.20525740804177417
1104	82-83	0.23601268532154052
1104	84-85	0.29655254943784826
1104	86-87	0.30731288767123743
1104	88-89	0.2572293931336489
1104	90-91	0.2585287494292601
1104	92-93	0.2471278496668674
1104	94-95	0.29441419402309776
1104	96-97	0.27703784794999464
1104	98-99	0.2864687495682787
1104	100	0.3198857800592876
1105	1	0.18542165384105047
1105	2	0.21669556706345006
1105	3	0.20817677701726112
1105	4	0.21927894422432104
1105	5	0.2278751944191839
1105	6	0.27439207406518307
1105	7	0.20509786080980064
1105	8	0.3058184148791625
1105	9	0.38088179464134697
1105	10-11	0.4018098840428621
1105	12-13	0.2924680031259044
1105	14-15	0.43646387699702416
1105	16-17	0.3455507824035138
1105	18-19	0.41778804900649646
1105	20-21	0.40858387515318384
1105	22-23	0.4081234800379434
1105	24-25	0.4696784604226423
1105	26-27	0.3579906469675507
1105	28-29	0.3816751429311367
1105	30-31	0.3604240287433669
1105	32-33	0.44506824089254593
1105	34-35	0.4470537814754394
1105	36-37	0.44382881006733754
1105	38-39	0.4350358263063754
1105	40-41	0.5009363977289283
1105	42-43	0.47262048620191166
1105	44-45	0.3361415837988915
1105	46-47	0.37011080325291346
1105	48-49	0.3764008333533795
1105	50-51	0.3795056337477192
1105	52-53	0.4013995018042493
1105	54-55	0.37361747910765786
1105	56-57	0.3879816201049735
1105	58-59	0.31568811378710393
1105	60-61	0.3974504125134217
1105	62-63	0.33660281721166285
1105	64-65	0.45554606585630353
1105	66-67	0.40338393399814976
1105	68-69	0.372902921199568
1105	70-71	0.26926249683184267
1105	72-73	0.3237300495758504
1105	74-75	0.35013764689762894
1105	76-77	0.3020525781802803
1105	78-79	0.332290076906574
1105	80-81	0.3463493101308899
1105	82-83	0.32802273451795827
1105	84-85	0.27188715146431264
1105	86-87	0.20814496195377785
1105	88-89	0.2585724261498825
1105	90-91	0.2646987382663326
1105	92-93	0.30947930428651205
1105	94-95	0.3225741299092135
1105	96-97	0.24552050018420957
1105	98-99	0.2079365595947067
1105	100	0.3099894958078586
1106	1	0.15483192379340238
1106	2	0.17509449688744638
1106	3	0.1789543129089708
1106	4	0.21797024837577084
1106	5	0.10489163529804557
1106	6	0.1795657273729674
1106	7	0.21969557543486218
1106	8	0.25120183781506
1106	9	0.3517916350199144
1106	10-11	0.28960304226705347
1106	12-13	0.3246092861859253
1106	14-15	0.3435008157770909
1106	16-17	0.35081503020587945
1106	18-19	0.363593170073905
1106	20-21	0.35238730792794115
1106	22-23	0.4098236921588523
1106	24-25	0.43179668884697975
1106	26-27	0.40790627309926464
1106	28-29	0.3813582089514753
1106	30-31	0.3735011385833218
1106	32-33	0.377524468636274
1106	34-35	0.38585002588034456
1106	36-37	0.40350093822104327
1106	38-39	0.3423743553981211
1106	40-41	0.38113328258929613
1106	42-43	0.40783192145774194
1106	44-45	0.3613229399215072
1106	46-47	0.35343239919657776
1106	48-49	0.32652885759278405
1106	50-51	0.3117953150250017
1106	52-53	0.31127800310781595
1106	54-55	0.2534494259941127
1106	56-57	0.2699325096056171
1106	58-59	0.3428075985659689
1106	60-61	0.2867290204236781
1106	62-63	0.2655157175187526
1106	64-65	0.32348278928693475
1106	66-67	0.343602459789615
1106	68-69	0.2950844679358653
1106	70-71	0.2495338987620812
1106	72-73	0.2346322882349554
1106	74-75	0.22336247337639037
1106	76-77	0.26358133907881154
1106	78-79	0.24904898222635552
1106	80-81	0.25023055698898133
1106	82-83	0.22373037193061407
1106	84-85	0.2534639391850746
1106	86-87	0.2358886617148812
1106	88-89	0.11025661791605046
1106	90-91	0.21470471430705373
1106	92-93	0.2560297917210903
1106	94-95	0.24742118647679945
1106	96-97	0.23853076641886517
1106	98-99	0.23165265249352984
1106	100	0.3774910362892996
1107	1	0.1449889392950645
1107	2	0.19670542047174067
1107	3	0.16357331517784957
1107	4	0.2030328013576721
1107	5	0.14219597223939928
1107	6	0.17942386935908416
1107	7	0.21201244069696656
1107	8	0.22450613388313911
1107	9	0.254009076358642
1107	10-11	0.21839438770454223
1107	12-13	0.2943359250362718
1107	14-15	0.23561867928690816
1107	16-17	0.27674735951792684
1107	18-19	0.2327398594285981
1107	20-21	0.3341439983483099
1107	22-23	0.39087404131469583
1107	24-25	0.4006202995711803
1107	26-27	0.3549050536186513
1107	28-29	0.3844134546372189
1107	30-31	0.3588712345670757
1107	32-33	0.3260266507991858
1107	34-35	0.2960088037265649
1107	36-37	0.2761181343705914
1107	38-39	0.2706489030711694
1107	40-41	0.28309758401064045
1107	42-43	0.32993350094170637
1107	44-45	0.26901735265535365
1107	46-47	0.2673924703602282
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2216	92-93	-1.0865647754670356
2216	94-95	-1.1647400455670045
2216	96-97	-1.1094721333028836
2216	98-99	-1.11713427448802
2216	100	-1.2680461606053512
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1167.0
1	275.0
2	212.0
3	197.0
4	132.0
5	195.0
6	271.0
7	577.0
8	1714.0
9	2282.0
10	2717.0
11	3966.0
12	5799.0
13	7817.0
14	9917.0
15	10787.0
16	11845.0
17	13179.0
18	14658.0
19	16559.0
20	18413.0
21	20252.0
22	22614.0
23	26611.0
24	32387.0
25	39097.0
26	41445.0
27	38671.0
28	41008.0
29	49276.0
30	57155.0
31	66949.0
32	84368.0
33	112706.0
34	164080.0
35	247427.0
36	448937.0
37	866690.0
38	974635.0
39	272232.0
40	341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01935734058641	20.10732012339533	13.470755333466622	24.402567202551637
2	14.624453754818548	20.713746061454195	40.88621036771208	23.77558981601518
3	17.59927744555753	26.30127150047575	30.628038482571274	25.47141257139545
4	11.947473747875492	17.804084313768634	40.3377369962965	29.910704942059375
5	12.949639515575912	38.86195974295427	35.58276973540862	12.605631006061206
6	29.56734652692686	39.06735645620357	18.679679923056156	12.685617093813416
7	26.19662523346429	33.205142654730466	23.376135119469314	17.22209699233593
8	24.687290597880036	36.391669884711256	21.068312087082834	17.852727430325867
9	25.898139927129726	16.17128136355794	20.846538018088506	37.08404069122383
10-11	23.94145500328443	27.032778843965875	29.303839994504482	19.72192615824521
12-13	24.49282674790794	24.786467312699163	28.616526055569274	22.104179883823623
14-15	22.07431875370215	26.083061031591477	26.087421437229946	25.755198777476423
16-17	21.987061564835	28.759755581992774	26.83283652858594	22.420346324586287
18-19	21.82884526988354	28.667295647607528	28.0194640620845	21.484395020424436
20-21	22.97120879796091	27.7871785126435	27.4210753042464	21.820537385149187
22-23	22.874219057001476	27.61236811660973	27.121078548238586	22.392334278150205
24-25	22.54189879674287	27.830989626797624	27.419260056704047	22.207851519755454
26-27	22.454913806773803	27.995968249359994	27.016230841738626	22.532887102127578
28-29	22.307603858368005	28.14710668688348	27.166172717957636	22.37911673679088
30-31	22.718604381875735	27.8131531410411	27.11234123184086	22.355901245242304
32-33	22.581561033561513	28.065048901800978	26.83266528400829	22.520724780629223
34-35	22.754424329015958	28.027470685058997	27.16637511289686	22.05172987302819
36-37	22.730102143575305	27.85510031534177	27.0956822960842	22.319115244998724
38-39	22.58447158821091	27.89933789265952	27.282289759147936	22.23390075998163
40-41	22.684310906669335	27.56087520425467	27.404542725823344	22.35027116325265
42-43	22.512147305451897	27.610808518746637	27.249093229311338	22.62795094649013
44-45	22.535071037073784	27.998419862712154	27.131222914352293	22.33528618586177
46-47	22.507422480747575	27.749229574206492	27.018554491078866	22.724793453967067
48-49	22.563029793999547	27.60125339653903	27.25822123809609	22.57749557136533
50-51	22.502511303212724	27.687819045541072	27.053840784315618	22.755828866930585
52-53	22.38830004798758	27.71391511029092	27.091297715168476	22.806487126553023
54-55	22.396644023693536	27.731192341465196	27.04626745187116	22.82589618297011
56-57	22.596013138438707	27.593176997345825	27.057872978721093	22.752936885494375
58-59	22.460960381656598	27.612720544571896	27.305570186768836	22.620748887002673
60-61	22.46342445984467	27.692322350539555	27.179817918747023	22.664435270868744
62-63	22.43522008865008	27.57040273653105	27.167142839889046	22.827234334929823
64-65	22.17492134899013	27.637689088864132	27.134158902215354	23.053230659930385
66-67	22.46006248714177	27.47606406428384	27.04783885773687	23.01603459083752
68-69	22.266556731755326	27.451077488277143	27.20354948050655	23.078816299460982
70-71	22.31385926509552	27.196058135416777	27.276283604144613	23.213798995343087
72-73	22.38398089886555	27.28617508156524	27.385360449099966	22.944483570469252
74-75	22.32796260125985	27.44405260023185	27.250652027397898	22.9773327711104
76-77	22.230084842488964	27.35195642584458	27.26974559599698	23.148213135669476
78-79	22.13023205625847	27.289996373659616	27.356832699014554	23.22293887106736
80-81	22.156913242139044	27.292151278072346	27.42894405184947	23.121991427939143
82-83	22.349396498908554	27.110113197975334	27.660877145067747	22.87961315804837
84-85	22.456185461861704	27.194551687412634	27.300129049436027	23.04913380128964
86-87	22.00994275768269	27.346153304198868	27.50050827634935	23.143395661769098
88-89	22.32305392158639	26.869310603459002	27.468355840305243	23.339279634649372
90-91	22.313989585990726	26.90926746445405	27.518189490629847	23.258553458925373
92-93	22.416330209045658	26.914842853446896	27.420790665568816	23.24803627193863
94-95	21.91681926228617	26.884387046007348	27.66936771871987	23.529425972986616
96-97	21.971182580192043	26.881506885527124	27.65805929929265	23.48925123498819
98-99	21.877466422432832	27.012094084155997	27.46569101968253	23.644748473728647
100	22.319957940729974	26.867912174395453	27.224265575476608	23.58786430939797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1853.0
1	1237.5
2	604.0
3	575.0
4	531.0
5	469.5
6	434.5
7	444.0
8	440.5
9	446.0
10	446.5
11	417.5
12	450.0
13	496.5
14	567.5
15	674.0
16	783.5
17	982.0
18	1250.0
19	1580.5
20	2258.0
21	3397.5
22	4982.0
23	7201.0
24	10283.0
25	14232.5
26	19086.5
27	24812.0
28	30773.0
29	37725.0
30	46451.0
31	54915.0
32	63549.0
33	71449.0
34	78097.0
35	85641.5
36	94396.0
37	105520.0
38	112671.5
39	114559.5
40	116603.5
41	118274.0
42	120338.5
43	124789.5
44	130830.5
45	138865.5
46	145619.5
47	149979.0
48	152744.5
49	150632.5
50	146418.5
51	145723.5
52	143153.0
53	138651.0
54	131888.5
55	117114.0
56	104803.5
57	92911.0
58	79322.5
59	68470.5
60	55748.5
61	43425.0
62	33620.0
63	24727.5
64	17123.0
65	11616.0
66	8449.5
67	6378.0
68	4378.0
69	2944.5
70	2191.5
71	1602.0
72	1168.5
73	927.0
74	709.0
75	470.0
76	315.0
77	235.0
78	167.0
79	102.5
80	67.0
81	57.5
82	48.0
83	28.5
84	20.0
85	16.0
86	21.0
87	21.0
88	12.5
89	11.0
90	11.5
91	8.5
92	5.5
93	4.0
94	3.5
95	5.5
96	5.0
97	4.5
98	4.5
99	4.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08915260781432661
2	0.0615622218170508
3	0.07459324960585162
4	0.07545125966601958
5	0.07797166421776296
6	0.08596188290307703
7	0.08258346829116571
8	0.08518431128604984
9	0.07140252469460205
10-11	0.07743540793015799
12-13	0.06849333433434507
14-15	0.07621542487585667
16-17	0.07806550906809383
18-19	0.07767672325958021
20-21	0.06986078786773775
22-23	0.07334645373717007
24-25	0.07620201846866655
26-27	0.07086626840699707
28-29	0.0793257113439655
30-31	0.08302587972843982
32-33	0.08482233829191647
34-35	0.08742318128680059
36-37	0.0880935016463068
38-39	0.08986314739540321
40-41	0.08598869571745729
42-43	0.09183388925235149
44-45	0.08852250667639078
46-47	0.08610935338216841
48-49	0.09450176428318623
50-51	0.09135125859350701
52-53	0.096418880511374
54-55	0.08880404122738339
56-57	0.0950916461995517
58-59	0.09914038117096924
60-61	0.09762545715848518
62-63	0.09853709284741363
64-65	0.10096365254882614
66-67	0.10278692392668304
68-69	0.09971685668014457
70-71	0.10217022919593734
72-73	0.09942191572196184
74-75	0.10384603009470285
76-77	0.10117815506386811
78-79	0.10835058291058462
80-81	0.10326955458552751
82-83	0.10745235362884631
84-85	0.10339021225023864
86-87	0.10007882967427793
88-89	0.09959619901543344
90-91	0.09956938620105321
92-93	0.09341584530078616
94-95	0.09333540685764541
96-97	0.091458509851028
98-99	0.10444931841825847
100	0.24388935960274133
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3729560.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.37477944430127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.70697919363509	39.297155777696766
2	15.357565204872795	17.315587024523584
3	6.307023924843662	10.666712481389784
4	3.138529271187227	7.077355817706541
5	1.7612329112590137	4.964455846113577
6	1.0994243804088957	3.7187884176743444
7	0.6986736230591423	2.757129998245702
8	0.4566977744486218	2.059698904579544
9	0.3357752632859101	1.7036330769535832
>10	1.1190977906973458	9.47063085375446
>50	0.014829258921676363	0.5526129392368211
>100	0.004091333961645566	0.3512387621755429
>500	0.0	0.0
>1k	8.006941888444023E-5	0.06500009994974912
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.6812814380248608E-5	0.0
12-13	0.0	0.0	0.0	2.6812814380248608E-5	2.6812814380248608E-5
14-15	0.0	0.0	0.0	5.3625628760497216E-5	2.6812814380248608E-5
16-17	0.0	0.0	0.0	5.3625628760497216E-5	2.6812814380248608E-5
18-19	0.0	0.0	0.0	6.703203595062151E-5	2.6812814380248608E-5
20-21	0.0	0.0	0.0	8.043844314074582E-5	8.043844314074582E-5
22-23	0.0	0.0	0.0	1.6087688628149167E-4	8.043844314074582E-5
24-25	0.0	0.0	0.0	2.681281438024861E-4	8.043844314074582E-5
26-27	0.0	0.0	0.0	7.105395810765882E-4	8.043844314074582E-5
28-29	0.0	0.0	0.0	0.0028823775458767255	8.043844314074582E-5
30-31	0.0	0.0	0.0	0.00974645802722037	8.043844314074582E-5
32-33	0.0	0.0	0.0	0.02591458509851028	8.043844314074582E-5
34-35	0.0	0.0	0.0	0.052620148221237895	8.043844314074582E-5
36-37	0.0	0.0	0.0	0.09604350111005053	8.043844314074582E-5
38-39	0.0	0.0	0.0	0.1637726702345585	8.043844314074582E-5
40-41	0.0	0.0	0.0	0.23927755552933858	8.043844314074582E-5
42-43	0.0	0.0	0.0	0.31710174926801016	8.043844314074582E-5
44-45	0.0	0.0	0.0	0.4156388421154238	8.043844314074582E-5
46-47	0.0	0.0	0.0	0.5369132015572883	8.043844314074582E-5
48-49	0.0	0.0	0.0	0.675253917352181	8.043844314074582E-5
50-51	0.0	0.0	0.0	0.8189035703943629	8.043844314074582E-5
52-53	0.0	0.0	0.0	0.9641351794849794	8.043844314074582E-5
54-55	0.0	0.0	0.0	1.128350261156812	8.043844314074582E-5
56-57	0.0	0.0	0.0	1.3248345649352737	8.043844314074582E-5
58-59	0.0	0.0	0.0	1.5501962698012632	8.043844314074582E-5
60-61	0.0	0.0	0.0	1.7875567091024143	8.043844314074582E-5
62-63	0.0	0.0	0.0	2.0301322408005236	8.043844314074582E-5
64-65	0.0	0.0	0.0	2.2816364396872553	8.043844314074582E-5
66-67	0.0	0.0	0.0	2.5703836377481526	8.043844314074582E-5
68-69	0.0	0.0	0.0	2.8996449983376054	8.043844314074582E-5
70-71	0.0	0.0	0.0	3.258641770074754	8.043844314074582E-5
72-73	0.0	0.0	0.0	3.6180675468419867	8.043844314074582E-5
74-75	0.0	0.0	0.0	3.9821587533113827	8.043844314074582E-5
76-77	0.0	0.0	0.0	4.377942706378232	8.043844314074582E-5
78-79	0.0	0.0	0.0	4.819134160598033	8.043844314074582E-5
80-81	0.0	0.0	0.0	5.303266337047802	8.043844314074582E-5
82-83	0.0	0.0	0.0	5.8018506204485245	8.043844314074582E-5
84-85	0.0	0.0	0.0	6.292954128637158	8.043844314074582E-5
86-87	0.0	0.0	0.0	6.8041270283894075	8.043844314074582E-5
88	0.0	0.0	0.0	7.214148585892169	8.043844314074582E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3265	0.0	34.422947	1
GTATCAA	5165	0.0	33.231953	1
CAACGCA	5075	0.0	31.30809	5
ATCAACG	5190	0.0	31.068073	3
AACGCAG	5970	0.0	27.786665	6
TATCAAC	6245	0.0	27.023998	2
TCAACGC	6190	0.0	26.20089	4
GTACATG	10425	0.0	24.403637	1
ACGCAGA	6610	0.0	24.100311	7
TACATGG	10550	0.0	23.30434	2
CGCAGAG	6925	0.0	23.207314	8
ACATGGG	10425	0.0	22.952463	3
GTACTAG	1580	0.0	22.322226	1
CATGGGG	8260	0.0	21.11453	4
TAACGCC	665	0.0	19.793562	4
CTAACGC	700	0.0	19.47545	3
TCTAACG	685	0.0	19.215647	2
GCAGAGT	8285	0.0	18.546757	9
GTATATA	2495	0.0	18.470934	1
GTATAAG	2125	0.0	17.703712	1
>>END_MODULE