FastQCFastQC Report
Mon 16 Jan 2017
ERR1146625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3729560
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106690.2860659166228724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97730.2620416349381696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56240.15079526807451818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59900.059.4290281
GTATCAA113700.044.0058821
ATCAACG143900.034.4413573
CAACGCA144900.034.1227465
TATCAAC150050.033.1873132
AACGCAG153500.032.5784076
TCAACGC153750.032.2960364
ACGCAGA170900.028.9302947
CGCAGAG174100.028.3175648
CTAACGC5950.027.659963
TCTAACG7000.026.1986352
TAACGCC6350.025.9175994
GCAGAGT195450.024.9597859
GTGGTAT20400.023.9735741
GTACATG107350.023.830131
ACATGGG101750.023.6611583
TACATGG107400.023.2488982
GAGTACT136000.022.51440612-13
AGAGTAC177200.022.45164910-11
GTACTAG14150.021.933961
Produced by FastQC (version 0.11.5)