##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146625_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3729560
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.242372826821395	33.0	31.0	34.0	30.0	34.0
2	32.30907935520544	34.0	31.0	34.0	30.0	34.0
3	32.42998503844957	34.0	31.0	34.0	30.0	34.0
4	35.97358052960671	37.0	35.0	37.0	35.0	37.0
5	36.01401452182027	37.0	35.0	37.0	35.0	37.0
6	36.09212588080095	37.0	35.0	37.0	35.0	37.0
7	36.066213708855734	37.0	35.0	37.0	35.0	37.0
8	36.07466671671725	37.0	36.0	37.0	35.0	37.0
9	37.837759682107276	39.0	38.0	39.0	35.0	39.0
10-11	37.789042943403516	39.0	38.0	39.0	35.0	39.0
12-13	37.764498627183904	39.0	38.0	39.0	35.0	39.0
14-15	39.11090222439108	40.0	39.0	41.0	36.0	41.0
16-17	39.06259746458027	40.0	38.5	41.0	36.0	41.0
18-19	39.16289347805103	40.0	39.0	41.0	36.0	41.0
20-21	39.22221508703439	40.0	39.0	41.0	36.0	41.0
22-23	39.192070780467404	40.0	39.0	41.0	36.0	41.0
24-25	39.1842250828516	41.0	39.0	41.0	36.0	41.0
26-27	39.11028928345435	40.0	39.0	41.0	35.0	41.0
28-29	39.05045179592231	40.0	39.0	41.0	35.0	41.0
30-31	38.91191212904471	40.0	38.5	41.0	35.0	41.0
32-33	38.806886871373564	40.0	38.0	41.0	35.0	41.0
34-35	38.73741178584069	40.0	38.0	41.0	35.0	41.0
36-37	38.67048070013621	40.0	38.0	41.0	35.0	41.0
38-39	38.586086294361806	40.0	38.0	41.0	35.0	41.0
40-41	38.487876049721685	40.0	38.0	41.0	34.5	41.0
42-43	38.38146550799558	40.0	38.0	41.0	34.0	41.0
44-45	38.2424028571735	40.0	38.0	41.0	34.0	41.0
46-47	38.010615059149075	40.0	38.0	41.0	33.0	41.0
48-49	37.73684040476624	40.0	37.5	41.0	33.0	41.0
50-51	37.63488373963685	40.0	37.0	41.0	33.0	41.0
52-53	37.539709509969	40.0	37.0	41.0	33.0	41.0
54-55	37.391081521680846	40.0	36.5	41.0	32.0	41.0
56-57	37.24598558543099	40.0	36.0	41.0	32.0	41.0
58-59	37.07585010027992	39.0	36.0	41.0	32.0	41.0
60-61	36.88258628363668	39.0	35.0	41.0	31.5	41.0
62-63	36.65217116764444	39.0	35.0	41.0	31.0	41.0
64-65	36.390783229120856	38.5	35.0	40.5	31.0	41.0
66-67	36.13183498857774	37.5	35.0	40.0	31.0	41.0
68-69	35.84559224144403	37.0	35.0	40.0	31.0	41.0
70-71	35.5430550788833	36.5	35.0	39.5	30.5	41.0
72-73	35.221379599738306	36.0	35.0	39.0	30.0	41.0
74-75	34.903643325217985	36.0	35.0	39.0	30.0	40.5
76-77	34.59927203208957	35.0	34.0	37.5	30.0	39.5
78-79	34.30676366649149	35.0	34.0	37.0	30.0	39.0
80-81	34.039232375937104	35.0	34.0	37.0	29.0	39.0
82-83	33.78070630315641	35.0	34.0	36.0	29.0	38.0
84-85	33.559466130052876	35.0	34.0	36.0	29.0	37.0
86-87	33.36334312894819	35.0	34.0	35.5	29.0	37.0
88-89	33.16133659198404	35.0	34.0	35.0	29.0	36.5
90-91	33.069731147910204	35.0	34.0	35.0	29.0	36.0
92-93	32.96391880007293	35.0	34.0	35.0	29.0	36.0
94-95	32.88491256341231	35.0	34.0	35.0	29.0	36.0
96-97	32.82569646285353	35.0	34.0	35.0	29.0	35.5
98-99	32.756111310717614	35.0	34.0	35.0	29.0	35.0
100	32.02247798667939	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35007958014505647
1101	2	0.34417010791677427
1101	3	0.34473041991320486
1101	4	0.24875327513377243
1101	5	0.23727343080570762
1101	6	0.28243111674589017
1101	7	0.23325088663658988
1101	8	0.2777340176586378
1101	9	0.35072806195406514
1101	10-11	0.28914128652677107
1101	12-13	0.303629210271275
1101	14-15	0.48355349865824593
1101	16-17	0.5090638336659339
1101	18-19	0.4901511048477687
1101	20-21	0.45446746961815165
1101	22-23	0.4697379938904689
1101	24-25	0.44883452711375327
1101	26-27	0.44421186466905027
1101	28-29	0.4848417745366973
1101	30-31	0.49287036615281465
1101	32-33	0.4801664882676846
1101	34-35	0.4643005114256269
1101	36-37	0.4505651707452216
1101	38-39	0.45392442608441286
1101	40-41	0.4576288198960796
1101	42-43	0.5165733146378599
1101	44-45	0.5039875915109064
1101	46-47	0.5304431592697867
1101	48-49	0.5330334323152144
1101	50-51	0.5488240479542128
1101	52-53	0.4889212796118727
1101	54-55	0.5048938151136824
1101	56-57	0.44896059005289146
1101	58-59	0.4412713302843585
1101	60-61	0.37057932806980887
1101	62-63	0.42727679372401894
1101	64-65	0.392227457321205
1101	66-67	0.3596168141866585
1101	68-69	0.36810248645823407
1101	70-71	0.28980861931287194
1101	72-73	0.2719132810614653
1101	74-75	0.28064162347468624
1101	76-77	0.25144657109280644
1101	78-79	0.3455615386879174
1101	80-81	0.3048571869923933
1101	82-83	0.3503391398717497
1101	84-85	0.32142928265508175
1101	86-87	0.3439430367546521
1101	88-89	0.3933867304439147
1101	90-91	0.32891533227746805
1101	92-93	0.3502551327728085
1101	94-95	0.3899150977558321
1101	96-97	0.36394170196473397
1101	98-99	0.4112165558923806
1101	100	0.3902383764213937
1102	1	0.28139998666705424
1102	2	0.2500107089355552
1102	3	0.25871522510941247
1102	4	0.2025540558659813
1102	5	0.15728634371298966
1102	6	0.20430596928229505
1102	7	0.21228795768720943
1102	8	0.2055629523969955
1102	9	0.289717487918864
1102	10-11	0.26010116862347843
1102	12-13	0.3053543425258525
1102	14-15	0.39901788192147336
1102	16-17	0.5098433347052591
1102	18-19	0.42230563826355194
1102	20-21	0.38287594719345464
1102	22-23	0.37230646156359626
1102	24-25	0.41670240719289353
1102	26-27	0.408161402885419
1102	28-29	0.3759938658485993
1102	30-31	0.4224334824341085
1102	32-33	0.3918247913334838
1102	34-35	0.45329739237283917
1102	36-37	0.42761258291867676
1102	38-39	0.4506512829469216
1102	40-41	0.46983665944605946
1102	42-43	0.48388808123943505
1102	44-45	0.44615360897851986
1102	46-47	0.47282744712633473
1102	48-49	0.6400956406577194
1102	50-51	0.5952088553098207
1102	52-53	0.5603527306116689
1102	54-55	0.5864433414611128
1102	56-57	0.47201538046457614
1102	58-59	0.4549592916354257
1102	60-61	0.43071029574050357
1102	62-63	0.45881820484053293
1102	64-65	0.5119032085024386
1102	66-67	0.4696440626425584
1102	68-69	0.47562143141529845
1102	70-71	0.49923393085598633
1102	72-73	0.5268266465535589
1102	74-75	0.5064250772664778
1102	76-77	0.4606508935766698
1102	78-79	0.5008432684737585
1102	80-81	0.4814824013158585
1102	82-83	0.4551144027370668
1102	84-85	0.4686131810116123
1102	86-87	0.4905238369377827
1102	88-89	0.5581314913817224
1102	90-91	0.4959338337223045
1102	92-93	0.4408176726571611
1102	94-95	0.4432864412073627
1102	96-97	0.5177651564074495
1102	98-99	0.5221419753472887
1102	100	0.6227512189345319
1103	1	0.20953594775586737
1103	2	0.2047407358109794
1103	3	0.2044187007650322
1103	4	0.19506226993640752
1103	5	0.17079546425073033
1103	6	0.1814101714960259
1103	7	0.22970155627896105
1103	8	0.21800993185915019
1103	9	0.2696600720966771
1103	10-11	0.27705669965061475
1103	12-13	0.24945252180203425
1103	14-15	0.39434591136083696
1103	16-17	0.4463762856326525
1103	18-19	0.35389015214119723
1103	20-21	0.3795699946441786
1103	22-23	0.3603914315313048
1103	24-25	0.326340546450929
1103	26-27	0.37018513538846065
1103	28-29	0.36782308085845017
1103	30-31	0.3962998462345695
1103	32-33	0.35235654960431617
1103	34-35	0.38625006656128136
1103	36-37	0.37589983648970104
1103	38-39	0.36722371590366265
1103	40-41	0.35735077802332427
1103	42-43	0.37100461101316995
1103	44-45	0.32856427591589465
1103	46-47	0.38871363016286153
1103	48-49	0.4763679890980015
1103	50-51	0.4348518647285715
1103	52-53	0.3811641589332453
1103	54-55	0.42613595335149057
1103	56-57	0.39177890576221586
1103	58-59	0.38622421838142174
1103	60-61	0.3339378511164739
1103	62-63	0.3459751087190739
1103	64-65	0.3176124145344019
1103	66-67	0.3145321901179443
1103	68-69	0.28482967159980177
1103	70-71	0.2965737222883078
1103	72-73	0.22606076901112715
1103	74-75	0.21358180756383405
1103	76-77	0.1636967160433258
1103	78-79	0.17163743205908588
1103	80-81	0.2002539650703028
1103	82-83	0.14950885103947797
1103	84-85	0.2288421389894708
1103	86-87	0.23493312203707717
1103	88-89	0.3275439377459577
1103	90-91	0.26422552789554743
1103	92-93	0.28678771627986777
1103	94-95	0.26373983086888586
1103	96-97	0.279424016864553
1103	98-99	0.3264488541389525
1103	100	0.2214167970933616
1104	1	0.19567563034190272
1104	2	0.19826765016680525
1104	3	0.21269722500731092
1104	4	0.13825729317254343
1104	5	0.1072942613127239
1104	6	0.14061703546378368
1104	7	0.15633106671456432
1104	8	0.17452270985506146
1104	9	0.23218769883050072
1104	10-11	0.24343199282013472
1104	12-13	0.24720122535978817
1104	14-15	0.39353354863485634
1104	16-17	0.43782986578967353
1104	18-19	0.38485674634273437
1104	20-21	0.36172219087495705
1104	22-23	0.38204066541019444
1104	24-25	0.3557952551128736
1104	26-27	0.31729559249009043
1104	28-29	0.32925769988963793
1104	30-31	0.3748696265316127
1104	32-33	0.32890415191224065
1104	34-35	0.3607574513066609
1104	36-37	0.34953291029652434
1104	38-39	0.3475593112196407
1104	40-41	0.3001370806016652
1104	42-43	0.33129268554315416
1104	44-45	0.23872349710570973
1104	46-47	0.33473599944552035
1104	48-49	0.36342788037597273
1104	50-51	0.29774544616370235
1104	52-53	0.2924119969617891
1104	54-55	0.3110676510827233
1104	56-57	0.3069055165876762
1104	58-59	0.256543808733916
1104	60-61	0.16265998381091862
1104	62-63	0.21190002621580106
1104	64-65	0.22483938506155
1104	66-67	0.26690327250187806
1104	68-69	0.22013674587406484
1104	70-71	0.19708965408835155
1104	72-73	0.19368629455257746
1104	74-75	0.19849136052838645
1104	76-77	0.16569000955416868
1104	78-79	0.16562152528167928
1104	80-81	0.1606576833173463
1104	82-83	0.1401458363125272
1104	84-85	0.13307771444605265
1104	86-87	0.20018588820200023
1104	88-89	0.2042968314575333
1104	90-91	0.1868215272577629
1104	92-93	0.17212289737337727
1104	94-95	0.18611581091764862
1104	96-97	0.2444965989030834
1104	98-99	0.28476008767228933
1104	100	0.3080914985119705
1105	1	0.2509161743201034
1105	2	0.2269538360032115
1105	3	0.23966086211875393
1105	4	0.20639311126031146
1105	5	0.13890649653873766
1105	6	0.14159673142456342
1105	7	0.18604063770207802
1105	8	0.15632506985637917
1105	9	0.213716864863585
1105	10-11	0.23390807358574506
1105	12-13	0.25657822262562746
1105	14-15	0.34114246143447247
1105	16-17	0.4050327490031762
1105	18-19	0.3460577246392802
1105	20-21	0.3083998071449514
1105	22-23	0.3003193352040512
1105	24-25	0.2683644815735775
1105	26-27	0.3072208481358558
1105	28-29	0.32639829358136296
1105	30-31	0.2996540604826876
1105	32-33	0.2891152628583811
1105	34-35	0.2545808578927762
1105	36-37	0.3131618470808277
1105	38-39	0.24487423502040429
1105	40-41	0.3602195814938014
1105	42-43	0.3188300880125894
1105	44-45	0.341606847138209
1105	46-47	0.39666870341558536
1105	48-49	0.4862006148397384
1105	50-51	0.38664397170472853
1105	52-53	0.4086601945693573
1105	54-55	0.4030621360050901
1105	56-57	0.3529126403149476
1105	58-59	0.3686321298453734
1105	60-61	0.327150126693347
1105	62-63	0.3320057404035026
1105	64-65	0.32621591881238743
1105	66-67	0.2984307404590112
1105	68-69	0.33218124126648974
1105	70-71	0.35806909747099525
1105	72-73	0.3538169900827768
1105	74-75	0.2908045888131099
1105	76-77	0.30652670533388005
1105	78-79	0.31113060930537983
1105	80-81	0.3283099849440987
1105	82-83	0.26896005195588657
1105	84-85	0.2749914170315506
1105	86-87	0.3291659521076724
1105	88-89	0.34175538005825956
1105	90-91	0.28777574354622004
1105	92-93	0.3192204077209766
1105	94-95	0.2938886127924647
1105	96-97	0.2776061957969205
1105	98-99	0.2842031187600469
1105	100	0.39619747483481405
1106	1	0.1743514340691661
1106	2	0.1191793575941702
1106	3	0.1530977247185703
1106	4	0.14702880219741843
1106	5	0.15376927669723983
1106	6	0.18238339688026173
1106	7	0.17054884868419862
1106	8	0.16276262724227308
1106	9	0.17007794034220325
1106	10-11	0.2163713861581158
1106	12-13	0.19371309509789825
1106	14-15	0.3091503266541267
1106	16-17	0.36722326061585164
1106	18-19	0.2584769645227709
1106	20-21	0.257387801743846
1106	22-23	0.26988671061990743
1106	24-25	0.20744946739494452
1106	26-27	0.2366647926292771
1106	28-29	0.248726216627567
1106	30-31	0.25878537733301954
1106	32-33	0.2180803414200838
1106	34-35	0.20870589949270624
1106	36-37	0.22490997677574143
1106	38-39	0.24880671440224233
1106	40-41	0.19873207402782356
1106	42-43	0.25347160628239607
1106	44-45	0.2321145301416223
1106	46-47	0.21394914669594556
1106	48-49	0.2879584187091737
1106	50-51	0.2519624555416158
1106	52-53	0.22295382410995046
1106	54-55	0.2649279684284309
1106	56-57	0.2333004975872015
1106	58-59	0.19650320977056168
1106	60-61	0.20828920408070672
1106	62-63	0.1895956790011155
1106	64-65	0.20298960036165425
1106	66-67	0.15491754922554435
1106	68-69	0.11023550762962486
1106	70-71	0.15144813352014097
1106	72-73	0.21049318960785968
1106	74-75	0.13382091261701134
1106	76-77	0.1285670772800387
1106	78-79	0.08249974877018929
1106	80-81	0.10236894604570068
1106	82-83	0.10629307135005206
1106	84-85	0.13622502837170458
1106	86-87	0.1095911983605049
1106	88-89	0.10730113280540365
1106	90-91	0.09199182804759687
1106	92-93	0.0899045445369211
1106	94-95	0.11272732703547916
1106	96-97	0.13869307210167392
1106	98-99	0.1798546156318821
1106	100	0.2793375230638304
1107	1	0.1220330757971908
1107	2	0.09014400564988989
1107	3	0.09887686692920994
1107	4	0.09206576018382862
1107	5	0.08151027991723936
1107	6	0.08796922490514447
1107	7	0.08595416538252465
1107	8	0.06842758415928785
1107	9	0.1031950406004043
1107	10-11	0.10558541801611909
1107	12-13	0.11498013126611539
1107	14-15	0.2158216144817331
1107	16-17	0.2813369534748347
1107	18-19	0.2181578136372906
1107	20-21	0.12728448098272338
1107	22-23	0.1415198417009762
1107	24-25	0.161849558905665
1107	26-27	0.19071153216411574
1107	28-29	0.20225715184560755
1107	30-31	0.1857907877749554
1107	32-33	0.11239988987384919
1107	34-35	0.12167285417613272
1107	36-37	0.14551578681701471
1107	38-39	0.20122468866412646
1107	40-41	0.11281787150813471
1107	42-43	0.15678928298621742
1107	44-45	0.1842771725937311
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2216	60-61	-0.8932878122817485
2216	62-63	-0.935504693920997
2216	64-65	-0.8421307819832293
2216	66-67	-0.9021446932413539
2216	68-69	-0.8815952239484304
2216	70-71	-0.8208790102460455
2216	72-73	-0.798072845577515
2216	74-75	-0.7342402952182496
2216	76-77	-0.7498319985189852
2216	78-79	-0.7689766055329557
2216	80-81	-0.7546668537661958
2216	82-83	-0.7541191880899163
2216	84-85	-0.6854096444877413
2216	86-87	-0.7044761897331
2216	88-89	-0.6118528500468727
2216	90-91	-0.6427252256708229
2216	92-93	-0.6341544746566541
2216	94-95	-0.618698132349941
2216	96-97	-0.6402027075614427
2216	98-99	-0.6800884375971776
2216	100	-0.8395305327138658
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	30.0
8	137.0
9	230.0
10	252.0
11	168.0
12	193.0
13	272.0
14	483.0
15	963.0
16	1644.0
17	2971.0
18	5114.0
19	7696.0
20	11451.0
21	16669.0
22	23763.0
23	27524.0
24	25638.0
25	22620.0
26	21196.0
27	24301.0
28	29460.0
29	37049.0
30	46129.0
31	58727.0
32	74604.0
33	98363.0
34	141108.0
35	214772.0
36	380235.0
37	854198.0
38	1182007.0
39	418524.0
40	1065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63969450829784	20.174110857246742	13.886127841997627	24.300066792457788
2	14.9751356905403	21.129058985231573	40.16850241939679	23.72730290483134
3	17.609760531495613	26.40030931337311	31.12173556548764	24.868194589643632
4	12.121921814614328	18.204304000266124	39.57748171612742	30.096292468992132
5	12.53680125872371	38.51031186016755	35.705479459400905	13.247407421707836
6	29.178946374890703	39.38088014906455	18.168221031262206	13.271952444782539
7	25.400824762170338	33.69070346099808	23.398229281738328	17.510242495093255
8	25.119216763626508	35.53063089900098	20.848059189966836	18.50209314740568
9	26.07865270970302	15.029601347075793	21.0308990872918	37.86084685592939
10-11	24.067355970868398	26.55966202965606	29.15254300911797	20.22043899035757
12-13	25.115013567284077	24.380020699492704	28.88807258765109	21.61689314557213
14-15	22.366481180010986	25.374906976254696	27.064597592449612	25.194014251284703
16-17	21.41790180359754	28.233302391229387	27.742183633074912	22.60661217209816
18-19	21.172357338169657	27.341754536869555	30.242101334368666	21.24378679059212
20-21	22.257171386099746	26.45057948103039	30.031754552299155	21.260494580570708
22-23	22.049745814519675	26.324874784156844	29.80218846190972	21.823190939413763
24-25	21.730659916987527	26.552797112796146	30.085506065058613	21.631036905157714
26-27	21.723752056048667	26.724496785063163	29.65327267176997	21.8984784871182
28-29	21.5301431710635	26.890387457285502	29.782672725827275	21.796796645823726
30-31	22.01337667903947	26.535838568213517	29.716597214597307	21.734187538149705
32-33	21.999697282067558	26.737608305786402	29.41675585316116	21.845938558984884
34-35	22.09063659907769	26.77662711858499	29.663300132750493	21.469436149586826
36-37	22.06815419791758	26.620530719797188	29.523891239108124	21.787423843177105
38-39	22.032290201303304	26.613172762243092	29.6686585665212	21.685878469932405
40-41	22.123906381320882	26.455708200502826	29.589089359299226	21.83129605887707
42-43	22.081331309859607	26.523209172127544	29.29179313377449	22.103666384238355
44-45	22.140311537372003	26.927823336760483	28.987033643456765	21.944831482410752
46-47	22.27892834543485	26.79278520790656	28.534518817233135	22.393767629425454
48-49	22.494971253966288	26.863210064034398	28.427135333463106	22.21468334853621
50-51	22.293018479391673	27.080848679200763	28.10720299445511	22.518929846952457
52-53	22.22095307808789	27.153508569069036	28.064986436022068	22.56055191682101
54-55	22.307262372854815	27.20889180732612	27.87827632080102	22.605569499018046
56-57	22.454171159493093	27.04064142747268	27.923253073120087	22.58193433991414
58-59	22.200237053712733	27.058309823920386	28.272281701643998	22.46917142072288
60-61	22.322802258941735	27.142184519567326	28.06014102566165	22.474872195829292
62-63	22.26648877224697	27.02904342692006	28.056230867345228	22.648236933487738
64-65	21.959069811589643	26.984776026998464	28.127797341525927	22.928356819885963
66-67	22.231650010584474	26.885360670553048	28.007811196002912	22.87517812285956
68-69	22.05180017034608	26.80556320857152	28.192021566623538	22.95061505445886
70-71	22.152763821520853	26.564694339762273	28.136873106352866	23.145668732364
72-73	22.186205529055474	26.667343264484366	28.222789807648248	22.923661398811916
74-75	22.149256404701177	26.86205840649876	27.959378975119105	23.029306213680954
76-77	22.101478858180176	26.816717713786208	27.879556952564055	23.20224647546956
78-79	21.966130916749734	26.777235332010996	27.91958455640591	23.337049194833366
80-81	21.9435189066885	26.814717254676783	27.935439836858077	23.30632400177664
82-83	22.152439915431817	26.623318992850315	28.119122088946746	23.105119002771126
84-85	22.248084816917054	26.718771408477025	27.728534277292717	23.304609497313205
86-87	21.754992546037602	26.878277866557987	27.90248715666191	23.4642424307425
88-89	21.995717993543472	26.497321399843415	27.805464987826984	23.70149561878613
90-91	21.964269506894848	26.524955561105806	27.856694604282357	23.654080327716986
92-93	22.04899189072372	26.498958589172545	27.750930673785295	23.701118846318437
94-95	21.533671112729415	26.47525327756975	27.961664053977493	24.029411555723346
96-97	21.599563270888687	26.440169747339016	27.946533931905332	24.013733049866968
98-99	21.465328025817566	26.57400858118692	27.739684155263607	24.22097923773191
100	21.78885906684952	26.341086198990425	27.60215330448201	24.267901429678048
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	115.5
2	174.0
3	311.5
4	225.0
5	186.0
6	425.0
7	1072.0
8	2019.0
9	2528.5
10	2036.0
11	1475.5
12	1563.0
13	1864.5
14	2198.5
15	2592.5
16	3034.0
17	3580.5
18	4264.0
19	4909.0
20	5859.0
21	7113.5
22	8748.0
23	10892.0
24	13765.5
25	17468.5
26	22053.0
27	27475.5
28	32981.5
29	39411.0
30	47540.5
31	55660.5
32	64076.5
33	71399.5
34	77279.0
35	84076.0
36	92039.0
37	102529.5
38	109647.0
39	111989.5
40	113909.5
41	115015.0
42	116501.5
43	120836.5
44	127213.5
45	136274.0
46	144145.0
47	148333.0
48	151049.0
49	149611.0
50	145550.5
51	144217.0
52	141576.0
53	136960.5
54	130578.5
55	116378.0
56	103878.5
57	92313.5
58	79226.0
59	68753.0
60	55899.0
61	43232.0
62	33157.5
63	23988.5
64	16374.5
65	11038.0
66	7972.5
67	5863.0
68	3935.5
69	2661.5
70	1962.0
71	1347.0
72	923.0
73	728.0
74	553.0
75	365.5
76	219.0
77	140.5
78	92.0
79	49.0
80	25.0
81	14.0
82	7.5
83	5.5
84	6.0
85	3.0
86	2.0
87	1.0
88	0.5
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002600842994884115
2	0.0031639120968693355
3	2.4131532942223748E-4
4	0.05249949055652677
5	0.0018232713778569055
6	0.004531365630262015
7	0.0
8	2.4131532942223748E-4
9	0.0
10-11	5.362562876049722E-5
12-13	0.0
14-15	4.021922157037291E-5
16-17	3.7537940132348054E-4
18-19	1.6087688628149165E-4
20-21	4.290050300839778E-4
22-23	0.0
24-25	0.0
26-27	4.1559862289385345E-4
28-29	1.3406407190124306E-5
30-31	2.547217366123618E-4
32-33	4.1559862289385345E-4
34-35	1.8768970066174027E-4
36-37	6.703203595062151E-5
38-39	0.0010322933536395713
40-41	7.10539581076588E-4
42-43	0.0
44-45	6.301011379358423E-4
46-47	0.0
48-49	5.362562876049722E-5
50-51	0.0
52-53	3.351601797531076E-4
54-55	3.887858085136048E-4
56-57	9.384485033087013E-5
58-59	6.837267666963395E-4
60-61	6.971331738864638E-4
62-63	1.8768970066174027E-4
64-65	3.887858085136048E-4
66-67	0.0011127317967803171
68-69	0.002506998144553245
70-71	0.004008515749847167
72-73	0.0031236928752989626
74-75	0.0030030352105878443
76-77	0.0043570823367903985
78-79	0.001407672754963052
80-81	0.0012467958686815603
82-83	6.703203595062152E-4
84-85	5.630691019852209E-4
86-87	0.0
88-89	0.0
90-91	1.3406407190124306E-5
92-93	1.0725125752099444E-4
94-95	1.4747047909136735E-4
96-97	4.558178444642264E-4
98-99	1.6087688628149165E-4
100	0.07070539152071559
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3729560.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.304161814712444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.69599886628345	36.08478477776963
2	16.306634365782408	17.384229537740325
3	6.778005004229823	10.838876265791916
4	3.3459849864997935	7.134197005999337
5	1.9004085816102272	5.064984327410986
6	1.1426575690507441	3.6545042375692094
7	0.7396905286418131	2.7600008542073375
8	0.5061553547343527	2.158414954571449
9	0.34304250141872744	1.645703370445282
>10	1.2093370391515952	9.743969308874679
>50	0.019706269449035585	0.699691589074885
>100	0.010708238494343644	1.0373882026374972
>500	0.0010859515249730597	0.3994115770896641
>1k	3.3413893076089527E-4	0.23319046033649218
>5k	1.6706946538044763E-4	0.6855897494368857
>10k+	8.353473269022382E-5	0.47506378104449787
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10669	0.2860659166228724	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9773	0.2620416349381696	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5624	0.15079526807451818	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.6812814380248608E-5	0.0
12-13	0.0	0.0	0.0	2.6812814380248608E-5	1.0725125752099443E-4
14-15	0.0	0.0	0.0	5.3625628760497216E-5	1.2065766471111874E-4
16-17	0.0	0.0	0.0	5.3625628760497216E-5	1.3406407190124305E-4
18-19	0.0	0.0	0.0	6.703203595062151E-5	1.6087688628149165E-4
20-21	0.0	0.0	0.0	8.043844314074582E-5	1.6087688628149165E-4
22-23	0.0	0.0	0.0	1.7428329347161594E-4	1.6087688628149165E-4
24-25	0.0	0.0	0.0	2.8153455099261043E-4	1.6087688628149165E-4
26-27	0.0	0.0	0.0	7.239459882667124E-4	1.6087688628149165E-4
28-29	0.0	0.0	0.0	0.0029762223962075955	1.6087688628149165E-4
30-31	0.0	0.0	0.0	0.00993414772788211	1.6087688628149165E-4
32-33	0.0	0.0	0.0	0.02624974527826339	1.6087688628149165E-4
34-35	0.0	0.0	0.0	0.053210030137603365	1.6087688628149165E-4
36-37	0.0	0.0	0.0	0.09720985853559133	1.6087688628149165E-4
38-39	0.0	0.0	0.0	0.16532781346861292	1.6087688628149165E-4
40-41	0.0	0.0	0.0	0.2410606076856251	1.6087688628149165E-4
42-43	0.0	0.0	0.0	0.3191395231609091	1.6087688628149165E-4
44-45	0.0	0.0	0.0	0.4174218942717103	1.6087688628149165E-4
46-47	0.0	0.0	0.0	0.5383476871266315	1.6087688628149165E-4
48-49	0.0	0.0	0.0	0.677197846394749	1.6087688628149165E-4
50-51	0.0	0.0	0.0	0.8222953914134643	1.6087688628149165E-4
52-53	0.0	0.0	0.0	0.968733577151192	1.6087688628149165E-4
54-55	0.0	0.0	0.0	1.1349328070871632	1.6087688628149165E-4
56-57	0.0	0.0	0.0	1.333924109010178	1.6087688628149165E-4
58-59	0.0	0.0	0.0	1.5622486298651852	1.6087688628149165E-4
60-61	0.0	0.0	0.0	1.8017809071311361	1.6087688628149165E-4
62-63	0.0	0.0	0.0	2.0464344319437147	1.6087688628149165E-4
64-65	0.0	0.0	0.0	2.301129355741696	1.6087688628149165E-4
66-67	0.0	0.0	0.0	2.59277233775566	1.6087688628149165E-4
68-69	0.0	0.0	0.0	2.9261494653524815	1.6087688628149165E-4
70-71	0.0	0.0	0.0	3.288940250324435	2.0109610785186457E-4
72-73	0.0	0.0	0.0	3.653031456793831	2.4131532942223748E-4
74-75	0.0	0.0	0.0	4.021466339192827	2.681281438024861E-4
76-77	0.0	0.0	0.0	4.420186295434314	2.681281438024861E-4
78-79	0.0	0.0	0.0	4.865319233368011	2.681281438024861E-4
80-81	0.0	0.0	0.0	5.351824343890431	2.681281438024861E-4
82-83	0.0	0.0	0.0	5.852540246034385	2.681281438024861E-4
84-85	0.0	0.0	0.0	6.34699535602055	2.681281438024861E-4
86-87	0.0	0.0	0.0	6.860514377031071	2.681281438024861E-4
88	0.0	0.0	0.0	7.272305580282929	2.681281438024861E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5990	0.0	59.429028	1
GTATCAA	11370	0.0	44.005882	1
ATCAACG	14390	0.0	34.441357	3
CAACGCA	14490	0.0	34.122746	5
TATCAAC	15005	0.0	33.187313	2
AACGCAG	15350	0.0	32.578407	6
TCAACGC	15375	0.0	32.296036	4
ACGCAGA	17090	0.0	28.930294	7
CGCAGAG	17410	0.0	28.317564	8
CTAACGC	595	0.0	27.65996	3
TCTAACG	700	0.0	26.198635	2
TAACGCC	635	0.0	25.917599	4
GCAGAGT	19545	0.0	24.959785	9
GTGGTAT	2040	0.0	23.973574	1
GTACATG	10735	0.0	23.83013	1
ACATGGG	10175	0.0	23.661158	3
TACATGG	10740	0.0	23.248898	2
GAGTACT	13600	0.0	22.514406	12-13
AGAGTAC	17720	0.0	22.451649	10-11
GTACTAG	1415	0.0	21.93396	1
>>END_MODULE