FastQCFastQC Report
Mon 16 Jan 2017
ERR1146624_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146624_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4330301
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.041.1664121
GTATCAA70050.033.0328451
CAACGCA73850.029.7939915
ATCAACG76950.028.5950343
AACGCAG86700.026.9401446
TATCAAC87950.026.1947122
GTACATG152850.025.9696451
TACATGG152800.025.416172
TCAACGC88400.025.2100934
ACATGGG152050.024.521113
ACGCAGA92100.023.879977
CGCAGAG92600.023.497288
GTACTAG23100.023.0067621
TGATCGC14800.022.2271549
CATGGGG119250.021.9212234
GTATAAG28250.021.4763411
GTGATCG14900.021.447188
ATGGGGG80250.020.9149345
GCTATTG27750.020.8300198
GCATCGT20500.020.6317568
Produced by FastQC (version 0.11.5)