##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146624_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4330301
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.080279638759524	31.0	31.0	34.0	28.0	34.0
2	31.316486775399678	31.0	31.0	34.0	28.0	34.0
3	31.443308675309176	33.0	31.0	34.0	28.0	34.0
4	34.942879028501714	37.0	35.0	37.0	32.0	37.0
5	34.97432280111706	37.0	35.0	37.0	32.0	37.0
6	35.04086459578676	37.0	35.0	37.0	32.0	37.0
7	34.9361443927339	37.0	35.0	37.0	32.0	37.0
8	34.940700196129555	37.0	35.0	37.0	32.0	37.0
9	36.54416171069863	39.0	37.0	39.0	32.0	39.0
10-11	36.51606897534374	39.0	37.0	39.0	32.0	39.0
12-13	36.46259428617087	39.0	37.0	39.0	32.0	39.0
14-15	37.79728614708308	40.0	38.0	41.0	32.0	41.0
16-17	37.83022588960905	40.0	38.0	41.0	32.0	41.0
18-19	37.854378829554804	40.0	38.0	41.0	32.0	41.0
20-21	37.95755860851243	40.0	38.0	41.0	32.0	41.0
22-23	37.973864980748445	40.0	38.0	41.0	32.0	41.0
24-25	37.99656790601854	40.0	38.0	41.0	32.0	41.0
26-27	38.05405351729591	40.0	38.0	41.0	32.0	41.0
28-29	38.03012827514762	40.0	38.0	41.0	32.0	41.0
30-31	37.993894766206786	40.0	38.0	41.0	32.0	41.0
32-33	37.96105490126437	40.0	38.0	41.0	32.0	41.0
34-35	37.909037270157434	40.0	38.0	41.0	31.5	41.0
36-37	37.83844644979644	40.0	38.0	41.0	31.0	41.0
38-39	37.77987869665411	40.0	38.0	41.0	31.0	41.0
40-41	37.72797306699927	40.0	38.0	41.0	31.0	41.0
42-43	37.57912209797887	40.0	37.0	41.0	30.5	41.0
44-45	37.4671483575853	40.0	37.0	41.0	30.0	41.0
46-47	37.360878839600296	40.0	37.0	41.0	30.0	41.0
48-49	37.253757302321475	40.0	36.5	41.0	30.0	41.0
50-51	37.12308278339081	40.0	36.0	41.0	30.0	41.0
52-53	36.993068380235	40.0	36.0	41.0	30.0	41.0
54-55	36.87418634409017	39.0	35.0	41.0	30.0	41.0
56-57	36.68191264302412	39.0	35.0	41.0	30.0	41.0
58-59	36.48315960022178	39.0	35.0	41.0	30.0	41.0
60-61	36.26780540198014	38.5	35.0	40.5	29.0	41.0
62-63	36.034324981104085	38.0	35.0	40.0	29.0	41.0
64-65	35.74798980486576	37.0	35.0	40.0	29.0	41.0
66-67	35.51482275712473	37.0	34.5	40.0	29.0	41.0
68-69	35.260412151487856	36.5	34.0	39.5	29.0	41.0
70-71	34.989867217082605	36.0	34.0	39.0	28.5	41.0
72-73	34.65361403283513	35.5	34.0	39.0	28.0	41.0
74-75	34.326853722177745	35.0	34.0	38.5	28.0	40.0
76-77	34.058747879189	35.0	34.0	37.0	28.0	39.5
78-79	33.74729112364244	35.0	34.0	37.0	27.0	39.0
80-81	33.507158509304546	35.0	33.0	36.0	27.0	39.0
82-83	33.232462824177816	35.0	33.0	36.0	27.0	37.5
84-85	33.01758595995983	35.0	33.0	36.0	27.0	37.0
86-87	32.808720109756806	35.0	33.0	35.0	26.0	37.0
88-89	32.63144409591851	35.0	33.0	35.0	26.0	36.0
90-91	32.47967081272179	35.0	33.0	35.0	26.0	36.0
92-93	32.32292801354917	35.0	33.0	35.0	25.5	36.0
94-95	32.19613243975419	35.0	33.0	35.0	25.0	36.0
96-97	32.09766849925675	35.0	33.0	35.0	25.0	35.0
98-99	31.98657933940389	35.0	33.0	35.0	25.0	35.0
100	30.834864366241515	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25590893478133836
1101	2	0.2662914993441561
1101	3	0.31133104640047193
1101	4	0.28577072288399563
1101	5	0.15844059132929544
1101	6	0.3422441513743024
1101	7	0.30885779537170777
1101	8	0.3511531207601877
1101	9	0.497063840909469
1101	10-11	0.511504321057842
1101	12-13	0.4975164935365868
1101	14-15	0.6070185018064578
1101	16-17	0.5623185321848894
1101	18-19	0.5325700388083945
1101	20-21	0.5023557873294422
1101	22-23	0.5177128928535168
1101	24-25	0.6059248501083729
1101	26-27	0.5380694219713078
1101	28-29	0.536346200762047
1101	30-31	0.5603447970131512
1101	32-33	0.5922938377424103
1101	34-35	0.531313235528394
1101	36-37	0.5179395322588434
1101	38-39	0.5823207413087559
1101	40-41	0.5562233969724772
1101	42-43	0.5817682214240563
1101	44-45	0.5292423085914422
1101	46-47	0.4550247064528179
1101	48-49	0.4705299730071033
1101	50-51	0.4358921644217162
1101	52-53	0.42966964598196
1101	54-55	0.4226581621205838
1101	56-57	0.45481371507818125
1101	58-59	0.5153809737066339
1101	60-61	0.4463327475269381
1101	62-63	0.38641168472683773
1101	64-65	0.47724148009412914
1101	66-67	0.39683835557552527
1101	68-69	0.4515163362331336
1101	70-71	0.38444680406074383
1101	72-73	0.34559051252476536
1101	74-75	0.38493054759879186
1101	76-77	0.3581003571318391
1101	78-79	0.30352533372197144
1101	80-81	0.3356692590023016
1101	82-83	0.3670224589356721
1101	84-85	0.32037911610570546
1101	86-87	0.31306200463342293
1101	88-89	0.344679156915511
1101	90-91	0.34606480548318785
1101	92-93	0.32490732401060995
1101	94-95	0.2973354906417569
1101	96-97	0.31359092481132933
1101	98-99	0.34961801629597744
1101	100	0.3854986369349227
1102	1	0.19915797356825138
1102	2	0.273235781716032
1102	3	0.32940989650695585
1102	4	0.26847648210339514
1102	5	0.2593396908815677
1102	6	0.388905711943643
1102	7	0.3527359387036384
1102	8	0.32995641552616206
1102	9	0.39921935198182723
1102	10-11	0.4368430319215548
1102	12-13	0.4122211700196843
1102	14-15	0.550159431740326
1102	16-17	0.5080887676920227
1102	18-19	0.45922785144437483
1102	20-21	0.42946077545124695
1102	22-23	0.44081424329292673
1102	24-25	0.5046312288902257
1102	26-27	0.46716364594462334
1102	28-29	0.48902033323606275
1102	30-31	0.5108565427097318
1102	32-33	0.5005076688747963
1102	34-35	0.48646808943913555
1102	36-37	0.5126178563664041
1102	38-39	0.4979904432222213
1102	40-41	0.45616458647544533
1102	42-43	0.4750872530910968
1102	44-45	0.4300009430314802
1102	46-47	0.43856650047856505
1102	48-49	0.38024851998937237
1102	50-51	0.4134430622405034
1102	52-53	0.37786526773069795
1102	54-55	0.393804812521239
1102	56-57	0.419019738165872
1102	58-59	0.43000510407599535
1102	60-61	0.442283885980423
1102	62-63	0.4462503168719749
1102	64-65	0.4343906779510007
1102	66-67	0.4433118753478169
1102	68-69	0.3709695633880088
1102	70-71	0.3273718009037623
1102	72-73	0.35822570663760445
1102	74-75	0.4431803226404014
1102	76-77	0.38041784723284877
1102	78-79	0.3280382559280852
1102	80-81	0.2875152509980268
1102	82-83	0.3494377158705859
1102	84-85	0.31938892469596425
1102	86-87	0.32173388654985047
1102	88-89	0.3155776527838654
1102	90-91	0.25852699846759464
1102	92-93	0.2771590874641845
1102	94-95	0.2883574975417318
1102	96-97	0.2652339411996323
1102	98-99	0.34038587676380416
1102	100	0.24816641949307083
1103	1	0.1887223201517223
1103	2	0.21227875384320427
1103	3	0.29204159510678807
1103	4	0.25067463338717744
1103	5	0.1848465558180763
1103	6	0.2558466834930684
1103	7	0.2698238732114788
1103	8	0.2807826073492876
1103	9	0.3727902831658483
1103	10-11	0.35324744343434133
1103	12-13	0.39883899551333
1103	14-15	0.4929267956393062
1103	16-17	0.46012442037519463
1103	18-19	0.48235987896485
1103	20-21	0.3900173835477716
1103	22-23	0.3761446260612189
1103	24-25	0.4480376132393502
1103	26-27	0.4121702025860685
1103	28-29	0.4183919727711185
1103	30-31	0.42376821345128235
1103	32-33	0.46358467939005976
1103	34-35	0.45776933353950966
1103	36-37	0.4468350882559662
1103	38-39	0.4299110745160988
1103	40-41	0.35426084867417984
1103	42-43	0.417239774298487
1103	44-45	0.29602694111169114
1103	46-47	0.3392368775335797
1103	48-49	0.30433992612540095
1103	50-51	0.3427231184887347
1103	52-53	0.3073719474953762
1103	54-55	0.24957859590158904
1103	56-57	0.35575768758715043
1103	58-59	0.33661039036528706
1103	60-61	0.3245520095308265
1103	62-63	0.33500853573431044
1103	64-65	0.24751184438690643
1103	66-67	0.2808269821499181
1103	68-69	0.26916292335048553
1103	70-71	0.3170218009209549
1103	72-73	0.2536682449859953
1103	74-75	0.3246116064140665
1103	76-77	0.3077881613454778
1103	78-79	0.28465596626227097
1103	80-81	0.18708193056331623
1103	82-83	0.20872278871725314
1103	84-85	0.2940861842100517
1103	86-87	0.25634695761905846
1103	88-89	0.18246075813392082
1103	90-91	0.26042480928041556
1103	92-93	0.24840455656942595
1103	94-95	0.217287807043391
1103	96-97	0.25101133176404744
1103	98-99	0.2762328729179373
1103	100	0.331646920130801
1104	1	0.109834190673471
1104	2	0.15680387053266287
1104	3	0.1479691898311266
1104	4	0.15732521621607276
1104	5	0.17232346338415994
1104	6	0.184767482642485
1104	7	0.261577451047188
1104	8	0.239314436904273
1104	9	0.2708808237050633
1104	10-11	0.3068091486298954
1104	12-13	0.2753642430776253
1104	14-15	0.35515353176589315
1104	16-17	0.35801396288755427
1104	18-19	0.30061465979200364
1104	20-21	0.38994942616393047
1104	22-23	0.3452857215544043
1104	24-25	0.3640723493380378
1104	26-27	0.3724005580937728
1104	28-29	0.32012347079105297
1104	30-31	0.3216880364335353
1104	32-33	0.35336927205494106
1104	34-35	0.3400778375595195
1104	36-37	0.3456549890112228
1104	38-39	0.33274679488943093
1104	40-41	0.3288334404546518
1104	42-43	0.3307488767466822
1104	44-45	0.3036686801363757
1104	46-47	0.335454747407816
1104	48-49	0.2871578367332077
1104	50-51	0.2710229977373615
1104	52-53	0.2728938661967035
1104	54-55	0.2777678063667324
1104	56-57	0.2430251191396664
1104	58-59	0.23984960515115716
1104	60-61	0.2678093239465582
1104	62-63	0.23572839635534137
1104	64-65	0.33245347976875905
1104	66-67	0.2855059072806796
1104	68-69	0.275899108781708
1104	70-71	0.25650120785853403
1104	72-73	0.27922152896901764
1104	74-75	0.21104803453582832
1104	76-77	0.20173455464320256
1104	78-79	0.2628371918369723
1104	80-81	0.18976361147565513
1104	82-83	0.17207257748135163
1104	84-85	0.19212632540666874
1104	86-87	0.18373162567046109
1104	88-89	0.1760925151465429
1104	90-91	0.21096918756338567
1104	92-93	0.20868915266690635
1104	94-95	0.16143980514031142
1104	96-97	0.22696075185559295
1104	98-99	0.21765552867015714
1104	100	0.2152080657574409
1105	1	0.1843301383580247
1105	2	0.1549276099926189
1105	3	0.1950883101015961
1105	4	0.25268359306457455
1105	5	0.13081474367082535
1105	6	0.1580972305084316
1105	7	0.18766827948453368
1105	8	0.24510251247709647
1105	9	0.2921713795355245
1105	10-11	0.29620336550922133
1105	12-13	0.31790422292084486
1105	14-15	0.38391172313323807
1105	16-17	0.368494571742616
1105	18-19	0.34623769558981365
1105	20-21	0.35983062365641416
1105	22-23	0.3255021164210987
1105	24-25	0.4364710899181361
1105	26-27	0.3921753851738572
1105	28-29	0.37263485506245786
1105	30-31	0.3425002592578039
1105	32-33	0.3291724458793155
1105	34-35	0.36423538806968025
1105	36-37	0.315010547395687
1105	38-39	0.38098569054897524
1105	40-41	0.40691226463996344
1105	42-43	0.39427321175307384
1105	44-45	0.32754415995817254
1105	46-47	0.28936687437728636
1105	48-49	0.28651879188027607
1105	50-51	0.34420325574355104
1105	52-53	0.26738978191419704
1105	54-55	0.23032344697875118
1105	56-57	0.24579287412509387
1105	58-59	0.17620932508047815
1105	60-61	0.2929681010124128
1105	62-63	0.3134107386915801
1105	64-65	0.2980088371628682
1105	66-67	0.2836600927930988
1105	68-69	0.2017941904990721
1105	70-71	0.24335676276271556
1105	72-73	0.2687649727531962
1105	74-75	0.31559673664458643
1105	76-77	0.2719491583229967
1105	78-79	0.28366655828942555
1105	80-81	0.1969430922696631
1105	82-83	0.22201062582287534
1105	84-85	0.2499302054969661
1105	86-87	0.23925602192987583
1105	88-89	0.21180037246061545
1105	90-91	0.18729350190462668
1105	92-93	0.2441294018120317
1105	94-95	0.26354600427110597
1105	96-97	0.2438838330620925
1105	98-99	0.18882840187160355
1105	100	0.34260200586384215
1106	1	0.015165706541669266
1106	2	0.08168817128315808
1106	3	0.05164786468943561
1106	4	0.04485381509061881
1106	5	0.020492838675764347
1106	6	0.08333166414234228
1106	7	0.13747338075447146
1106	8	0.13081020271138755
1106	9	0.14434471736946364
1106	10-11	0.1659522462294163
1106	12-13	0.1804215607844597
1106	14-15	0.20301471665803916
1106	16-17	0.2056295121898799
1106	18-19	0.2590275430017712
1106	20-21	0.24378047893208077
1106	22-23	0.17710890888232456
1106	24-25	0.2478630647344957
1106	26-27	0.23355628156211594
1106	28-29	0.2351449968935384
1106	30-31	0.2240980381318849
1106	32-33	0.30954208950260664
1106	34-35	0.22087087192867472
1106	36-37	0.2216468596639345
1106	38-39	0.1902925098407806
1106	40-41	0.18857760214255137
1106	42-43	0.24018768282613223
1106	44-45	0.24211919219812472
1106	46-47	0.2593218064415481
1106	48-49	0.20964121469292962
1106	50-51	0.22599468455166516
1106	52-53	0.18397784861721078
1106	54-55	0.13212895874401198
1106	56-57	0.18074348486053537
1106	58-59	0.1903392789986711
1106	60-61	0.24830717386173262
1106	62-63	0.17920938084227345
1106	64-65	0.27064971928271575
1106	66-67	0.237298107627808
1106	68-69	0.20728701363122326
1106	70-71	0.1375965736381204
1106	72-73	0.1702523259114841
1106	74-75	0.21953971735996447
1106	76-77	0.163502870771012
1106	78-79	0.19454148209432987
1106	80-81	0.16071696622039155
1106	82-83	0.14220800978066706
1106	84-85	0.19059095460148257
1106	86-87	0.2026329642095419
1106	88-89	0.18239453971768427
1106	90-91	0.18901906599568719
1106	92-93	0.15454469449396413
1106	94-95	0.14226670547625275
1106	96-97	0.1447860820077267
1106	98-99	0.21689663806468218
1106	100	0.22277770280948417
1107	1	0.0914274707624898
1107	2	0.08281939713022268
1107	3	0.09181512972368466
1107	4	0.07728170137600898
1107	5	0.04080539134631067
1107	6	0.10238637022334274
1107	7	0.11549250314465809
1107	8	0.11116898161863986
1107	9	0.15231638718655205
1107	10-11	0.19306053898188935
1107	12-13	0.22067530971897042
1107	14-15	0.2667096621585614
1107	16-17	0.21802367724508542
1107	18-19	0.2166725542592971
1107	20-21	0.2442714726089008
1107	22-23	0.24844071624239206
1107	24-25	0.27396009512769837
1107	26-27	0.23501393036715967
1107	28-29	0.20024242418478622
1107	30-31	0.24503905497618916
1107	32-33	0.27577184211491357
1107	34-35	0.27082769643028115
1107	36-37	0.23879594773482182
1107	38-39	0.25243962550363364
1107	40-41	0.20062331851328707
1107	42-43	0.20314442214869644
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2216	60-61	-0.43294804306771795
2216	62-63	-0.47446777805228635
2216	64-65	-0.46388574422829265
2216	66-67	-0.4676163511756215
2216	68-69	-0.5569212997889323
2216	70-71	-0.5512505937892769
2216	72-73	-0.4502077506712041
2216	74-75	-0.4319312777699338
2216	76-77	-0.45790348957780225
2216	78-79	-0.43012379274619406
2216	80-81	-0.40803724940688824
2216	82-83	-0.3694886701528475
2216	84-85	-0.44664056581057565
2216	86-87	-0.4624418964304766
2216	88-89	-0.45461908379656535
2216	90-91	-0.44286437856120386
2216	92-93	-0.3829935146166257
2216	94-95	-0.43188828255648914
2216	96-97	-0.4777462837548967
2216	98-99	-0.4196183369656481
2216	100	-0.49586230632316486
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1263.0
1	328.0
2	211.0
3	201.0
4	178.0
5	226.0
6	365.0
7	861.0
8	2463.0
9	3361.0
10	3467.0
11	4158.0
12	5441.0
13	7368.0
14	9694.0
15	10873.0
16	11827.0
17	13480.0
18	15077.0
19	16971.0
20	19044.0
21	20748.0
22	23300.0
23	27288.0
24	33540.0
25	40553.0
26	44605.0
27	42834.0
28	45423.0
29	55636.0
30	64737.0
31	74403.0
32	92115.0
33	123186.0
34	182035.0
35	275641.0
36	500339.0
37	1013853.0
38	1171997.0
39	370608.0
40	603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22285918362865	20.56200800028663	13.084872153755292	24.130260662329423
2	14.744796838276395	21.010081309981043	40.7375321576157	23.507589694126857
3	17.55010018882184	26.863639200061012	30.71655230272509	24.869708308392056
4	11.89133157989618	17.825868898986304	40.6968063715407	29.585993149576815
5	12.843421356133868	38.99080172751314	35.70923400425545	12.456542912097538
6	28.967681452927014	39.53725580111749	18.77328386448704	12.72177888146846
7	25.976700723745672	33.4033796986034	23.565971703795398	17.053947873855527
8	25.04373555871382	35.99961724885596	21.28260463078036	17.674042561649863
9	26.122099035756175	15.739417293993263	21.220297072604268	36.9181865976463
10-11	24.09037790271395	26.83497420394019	29.31458990291132	19.760057990434536
12-13	24.59338266958098	24.865498854445285	28.604828857741737	21.936289618232006
14-15	22.29195003863546	26.080293580966973	25.93996487391274	25.68779150648483
16-17	21.89070082103749	28.813422809175197	26.8341436318143	22.461732737973012
18-19	21.722785244706106	28.80287467143005	27.98401948556869	21.490320598295153
20-21	22.974265992603453	27.851845826353888	27.298970868484762	21.874917312557894
22-23	23.02792429911931	27.61854073718264	26.997687486300038	22.35584747739802
24-25	22.536260847690514	27.911721138969803	27.470516340882067	22.08150167245761
26-27	22.436992752953355	28.214675269453327	26.966500896637147	22.381831080956164
28-29	22.296987324599566	28.303444625868263	27.02482270732871	22.374745342203457
30-31	22.66703399615968	28.063865409487644	26.96899548018316	22.30010511416952
32-33	22.678779834197034	28.306077589280278	26.507893015864614	22.507249560658078
34-35	22.88279159607986	28.125031419531847	27.07411958571404	21.918057398674247
36-37	22.873400004830653	27.690428591091283	27.097032464651576	22.33913893942649
38-39	22.637084540908155	27.914676705861467	27.202913480591395	22.24532527263898
40-41	22.557719855944775	27.50494952936135	27.580735225256163	22.356595389437707
42-43	22.49713561788946	27.669630707932875	27.194387128551988	22.638846545625672
44-45	22.302149377389085	28.147218408264173	27.247465867081804	22.303166347264934
46-47	22.490667591770077	27.853478503999618	26.986448290490483	22.66940561373982
48-49	22.612050738125188	27.58804368365122	27.148040516996787	22.6518650612268
50-51	22.463432552681546	27.68134410988673	26.984347478782748	22.870875858648976
52-53	22.308726243915068	27.85267182277533	26.97157591083291	22.86702602247669
54-55	22.244676428872804	27.812244927204112	27.028150502584776	22.914928141338308
56-57	22.570902783179182	27.67046433627307	26.986148766837015	22.772484113710732
58-59	22.232769296648314	27.555602813845937	27.401815604443485	22.809812285062264
60-61	22.20500146318664	27.938809211095066	27.107393276368448	22.748796049349842
62-63	22.433463503803175	27.55034416505129	27.039898551173547	22.976293779971993
64-65	21.93730659261583	27.66458076988246	27.155592982676758	23.24251965482496
66-67	22.294725293675583	27.569928619097738	26.913089781266393	23.22225630596029
68-69	21.975960501039218	27.50215350425679	27.15751191867665	23.36437407602734
70-71	22.054079106976403	27.270160327925886	27.20920300671929	23.466557558378422
72-73	22.362722019942428	27.273480627543933	27.374402854670493	22.98939449784315
74-75	22.117612574517327	27.56782453503859	27.267414691914915	23.047148198529168
76-77	22.110729791020848	27.330949559454133	27.24174585450688	23.316574795018138
78-79	21.92622706855337	27.34517614474172	27.36369317989392	23.364903606810987
80-81	21.921120034027286	27.413718174547036	27.5499082280383	23.11525356338738
82-83	22.23356125836254	26.861009192710327	28.00016043406894	22.905269114858196
84-85	22.34402026388591	27.152555322015132	27.387106449549197	23.116317964549758
86-87	21.805777337245118	27.397472906162534	27.626710672674264	23.17003908391808
88-89	22.206601295322088	26.767504860896253	27.45237026697569	23.573523576805975
90-91	22.060589319345343	26.783731508283747	27.725505175860203	23.43017399651071
92-93	22.294275734876873	26.781755559032966	27.590164612696604	23.333804093393557
94-95	21.646967943789875	26.710095235277503	27.907886531288124	23.735050289644498
96-97	21.793040913430527	26.648110889950356	27.742169008039102	23.816679188580014
98-99	21.625503066045887	26.96549590213342	27.57533001907025	23.833671012750447
100	22.022174451923792	26.654483851596826	27.3080639463524	24.015277750126977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1972.0
1	1360.0
2	796.0
3	803.5
4	719.0
5	647.5
6	581.5
7	564.5
8	580.5
9	576.5
10	561.0
11	547.0
12	556.5
13	596.0
14	642.5
15	717.5
16	847.0
17	993.5
18	1226.5
19	1611.5
20	2200.5
21	3252.0
22	4923.5
23	7073.5
24	9331.5
25	12392.0
26	17829.5
27	24757.0
28	31381.0
29	39688.0
30	50667.5
31	61799.0
32	76082.5
33	88254.5
34	97122.5
35	105901.5
36	115974.5
37	131761.0
38	139187.0
39	137408.0
40	137583.5
41	136973.5
42	137924.0
43	143099.0
44	150044.0
45	162019.5
46	171364.5
47	179137.0
48	186768.5
49	182674.5
50	172018.0
51	166552.5
52	161081.5
53	154670.5
54	147069.0
55	131006.0
56	117881.5
57	102447.5
58	87724.0
59	78913.5
60	64100.0
61	49417.5
62	38625.0
63	29082.0
64	20202.5
65	13232.5
66	9158.0
67	6818.0
68	5336.5
69	3958.5
70	2842.0
71	2075.0
72	1510.5
73	1122.5
74	836.5
75	610.0
76	417.5
77	267.5
78	162.5
79	111.5
80	76.0
81	66.5
82	65.0
83	56.0
84	46.0
85	34.0
86	29.5
87	23.5
88	15.5
89	15.0
90	18.0
91	13.5
92	11.5
93	12.0
94	11.5
95	12.5
96	10.0
97	9.5
98	13.5
99	11.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09712950670172812
2	0.0643835151413262
3	0.08015609076597678
4	0.08223446822749736
5	0.08357386703603283
6	0.08791536662232025
7	0.08412810102577165
8	0.08613719923857487
9	0.07103432301819204
10-11	0.0780661667630033
12-13	0.06746644170924838
14-15	0.0766113025399389
16-17	0.07845874917240164
18-19	0.0786665869185537
20-21	0.06954481917076896
22-23	0.0737015740938101
24-25	0.07587232388695382
26-27	0.06976420345837392
28-29	0.07888597120615864
30-31	0.08260395755398989
32-33	0.0845091368937171
34-35	0.08711865526207069
36-37	0.08693391059882442
38-39	0.08756897037873348
40-41	0.0837586116992791
42-43	0.08978590633768876
44-45	0.08596400111678149
46-47	0.08261550409544278
48-49	0.09164489951160439
50-51	0.0879730993295847
52-53	0.09331914802227374
54-55	0.08584853570225257
56-57	0.09254552974492997
58-59	0.09684084316540582
60-61	0.09530515315217117
62-63	0.09650599346327195
64-65	0.09846890551026359
66-67	0.10054728297178418
68-69	0.09727961174061572
70-71	0.09984294394315776
72-73	0.09693321549702896
74-75	0.1010899704200701
76-77	0.09808786964231817
78-79	0.10517744609439389
80-81	0.10070893455212467
82-83	0.10476177060208978
84-85	0.10111306350297589
86-87	0.09756827527693802
88-89	0.0968870293312174
90-91	0.09718723940899258
92-93	0.09154098063852836
94-95	0.09151788755562257
96-97	0.0897281736304243
98-99	0.10282195163800391
100	0.2437474900705517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4330301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.6392338467214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.9450428465465	31.892105510454122
2	14.872968842576064	14.17073681372965
3	6.709621592431512	9.589236961945682
4	3.5780408724677337	6.818205033464697
5	2.1661920224895894	5.159786415814197
6	1.393354756798379	3.9827011854335757
7	0.9637123988613525	3.213736423123857
8	0.6782835807791268	2.585032809530269
9	0.4872420290307484	2.0890653264852155
>10	2.115692963368009	16.489917465575147
>50	0.06788993514091722	2.1417457430977356
>100	0.021475066903259777	1.6829265931796271
>500	3.864740854329227E-4	0.11120442916308429
>1k	9.661852135821706E-5	0.0735992890032077
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6186165811568294E-5
2	0.0	0.0	0.0	0.0	4.6186165811568294E-5
3	0.0	0.0	0.0	0.0	4.6186165811568294E-5
4	0.0	0.0	0.0	0.0	4.6186165811568294E-5
5	0.0	0.0	0.0	0.0	4.6186165811568294E-5
6	0.0	0.0	0.0	0.0	4.6186165811568294E-5
7	0.0	0.0	0.0	0.0	4.6186165811568294E-5
8	0.0	0.0	0.0	0.0	4.6186165811568294E-5
9	0.0	0.0	0.0	0.0	4.6186165811568294E-5
10-11	0.0	0.0	0.0	0.0	4.6186165811568294E-5
12-13	0.0	0.0	0.0	0.0	2.4247737051073353E-4
14-15	0.0	0.0	0.0	0.0	3.3484970213387016E-4
16-17	0.0	0.0	0.0	1.1546541452892073E-5	3.925824093983305E-4
18-19	0.0	0.0	0.0	3.463962435867622E-5	4.387685752098988E-4
20-21	0.0	0.0	0.0	9.23723316231366E-5	4.965012824743592E-4
22-23	0.0	0.0	0.0	2.0783774615205735E-4	5.542339897388196E-4
24-25	0.0	0.0	0.0	5.888736140974958E-4	5.542339897388196E-4
26-27	0.0	0.0	0.0	0.001096921438024747	6.1196669700328E-4
28-29	0.0	0.0	0.0	0.00479181470295021	6.466063213619561E-4
30-31	0.0	0.0	0.0	0.015680203293027436	6.696994042677403E-4
32-33	0.0	0.0	0.0	0.03955845101760824	6.927924871735244E-4
34-35	0.0	0.0	0.0	0.07774286360232233	7.85164818796661E-4
36-37	0.0	0.0	0.0	0.13887025405393297	7.85164818796661E-4
38-39	0.0	0.0	0.0	0.23270899644158682	8.313509846082294E-4
40-41	0.0	0.0	0.0	0.3369165330539378	8.544440675140135E-4
42-43	0.0	0.0	0.0	0.4418284086949152	8.544440675140135E-4
44-45	0.0	0.0	0.0	0.5762070581236731	9.35269857684258E-4
46-47	0.0	0.0	0.0	0.7429622097863404	0.0010391887307602866
48-49	0.0	0.0	0.0	0.9237348627728188	0.001085374896571855
50-51	0.0	0.0	0.0	1.1197258573942088	0.0011315610623834232
52-53	0.0	0.0	0.0	1.316247992922432	0.0012239333940065598
54-55	0.0	0.0	0.0	1.529443334308631	0.0012701195598181282
56-57	0.0	0.0	0.0	1.7892636100816088	0.0013047591841768044
58-59	0.0	0.0	0.0	2.0784582873107436	0.0013163057256296963
60-61	0.0	0.0	0.0	2.3845340081440067	0.0013163057256296963
62-63	0.0	0.0	0.0	2.695286078265691	0.001408678057252833
64-65	0.0	0.0	0.0	3.019178112560766	0.001420224598705725
66-67	0.0	0.0	0.0	3.3887251717605773	0.0014317711401586172
68-69	0.0	0.0	0.0	3.8030843583390626	0.0014317711401586172
70-71	0.0	0.0	0.0	4.252279460480922	0.0014433176816115094
72-73	0.0	0.0	0.0	4.706589680486415	0.0015010503888759695
74-75	0.0	0.0	0.0	5.156108085788956	0.0015818761790462141
76-77	0.0	0.0	0.0	5.661142262397002	0.0016280623448577825
78-79	0.0	0.0	0.0	6.215676462213597	0.0016857950521222429
80-81	0.0	0.0	0.0	6.808487446946529	0.001697341593575135
82-83	0.0	0.0	0.0	7.42728969648992	0.0017550743008395951
84-85	0.0	0.0	0.0	8.039060102288502	0.0017781673837453794
86-87	0.0	0.0	0.0	8.67313842617407	0.0019051793397271922
88	0.0	0.0	0.0	9.176660005851787	0.002032191295709005
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4250	0.0	41.166412	1
GTATCAA	7005	0.0	33.032845	1
CAACGCA	7385	0.0	29.793991	5
ATCAACG	7695	0.0	28.595034	3
AACGCAG	8670	0.0	26.940144	6
TATCAAC	8795	0.0	26.194712	2
GTACATG	15285	0.0	25.969645	1
TACATGG	15280	0.0	25.41617	2
TCAACGC	8840	0.0	25.210093	4
ACATGGG	15205	0.0	24.52111	3
ACGCAGA	9210	0.0	23.87997	7
CGCAGAG	9260	0.0	23.49728	8
GTACTAG	2310	0.0	23.006762	1
TGATCGC	1480	0.0	22.227154	9
CATGGGG	11925	0.0	21.921223	4
GTATAAG	2825	0.0	21.476341	1
GTGATCG	1490	0.0	21.44718	8
ATGGGGG	8025	0.0	20.914934	5
GCTATTG	2775	0.0	20.830019	8
GCATCGT	2050	0.0	20.631756	8
>>END_MODULE