FastQCFastQC Report
Mon 16 Jan 2017
ERR1146624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4330301
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136120.31434304451353384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122550.28300573101038473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74650.17238986389167865No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77600.061.8815081
GTATCAA150900.044.0090831
CAACGCA194800.033.4893265
ATCAACG195250.033.4322173
TATCAAC199150.033.156882
TCAACGC206600.031.6179494
AACGCAG210400.031.407296
ACGCAGA225350.028.9264227
CGCAGAG226800.028.6171558
GCAGAGT249050.025.9284179
GTGGTAT24900.025.8772491
GTACATG145000.023.8081111
TACATGG141750.023.6898332
GTACTAG21900.023.4088061
GAGTACT171800.023.25260412-13
ACATGGG140350.023.1878473
AGAGTAC231800.022.98179210-11
CAGAGTA242500.022.63637710-11
TGGTATC28450.021.3252582
AGTACTT184650.021.17628912-13
Produced by FastQC (version 0.11.5)