##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146624_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4330301
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.336395784034416	33.0	31.0	34.0	30.0	34.0
2	32.39331284361064	34.0	31.0	34.0	30.0	34.0
3	32.50262002572108	34.0	31.0	34.0	30.0	34.0
4	36.03080986748958	37.0	35.0	37.0	35.0	37.0
5	36.07511325425184	37.0	35.0	37.0	35.0	37.0
6	36.1511326810769	37.0	36.0	37.0	35.0	37.0
7	36.122906236771996	37.0	36.0	37.0	35.0	37.0
8	36.132624036989576	37.0	36.0	37.0	35.0	37.0
9	37.916433753681325	39.0	38.0	39.0	35.0	39.0
10-11	37.86798700598411	39.0	38.0	39.0	35.0	39.0
12-13	37.84391327531273	39.0	38.0	39.0	35.0	39.0
14-15	39.20082022011864	40.0	39.0	41.0	36.0	41.0
16-17	39.15274515559081	40.0	39.0	41.0	36.0	41.0
18-19	39.260411458695366	40.5	39.0	41.0	36.0	41.0
20-21	39.319395349191666	41.0	39.0	41.0	36.0	41.0
22-23	39.28636219514533	41.0	39.0	41.0	36.0	41.0
24-25	39.27327430587388	41.0	39.0	41.0	36.0	41.0
26-27	39.1950946366084	41.0	39.0	41.0	35.5	41.0
28-29	39.1360757600915	40.5	39.0	41.0	35.0	41.0
30-31	38.98716959860296	40.0	38.5	41.0	35.0	41.0
32-33	38.87234432433219	40.0	38.0	41.0	35.0	41.0
34-35	38.79756430326668	40.0	38.0	41.0	35.0	41.0
36-37	38.724234412342234	40.0	38.0	41.0	35.0	41.0
38-39	38.63319131857116	40.0	38.0	41.0	35.0	41.0
40-41	38.53054487436324	40.0	38.0	41.0	35.0	41.0
42-43	38.4180547726359	40.0	38.0	41.0	34.0	41.0
44-45	38.25847521915913	40.0	38.0	41.0	34.0	41.0
46-47	38.00212236978446	40.0	38.0	41.0	33.0	41.0
48-49	37.70418938544918	40.0	37.0	41.0	33.0	41.0
50-51	37.587781195810635	40.0	37.0	41.0	33.0	41.0
52-53	37.47843279254722	40.0	37.0	41.0	33.0	41.0
54-55	37.31662660401667	40.0	36.0	41.0	32.0	41.0
56-57	37.15070418892359	39.5	36.0	41.0	32.0	41.0
58-59	36.960407140288865	39.0	35.0	41.0	32.0	41.0
60-61	36.75660883619868	39.0	35.0	41.0	31.5	41.0
62-63	36.51602498302081	39.0	35.0	41.0	31.0	41.0
64-65	36.24408337896142	38.0	35.0	40.5	31.0	41.0
66-67	35.97827102550146	37.0	35.0	40.0	31.0	41.0
68-69	35.692012518298384	37.0	35.0	40.0	31.0	41.0
70-71	35.398203727639256	36.0	35.0	39.0	30.5	41.0
72-73	35.0892622706828	36.0	35.0	39.0	30.0	41.0
74-75	34.77706087867794	35.0	35.0	39.0	30.0	40.5
76-77	34.48860183160478	35.0	34.5	37.0	30.0	39.5
78-79	34.21210557880387	35.0	34.0	37.0	30.0	39.0
80-81	33.959381691942426	35.0	34.0	36.5	29.5	39.0
82-83	33.711973024508	35.0	34.0	36.0	29.0	38.0
84-85	33.50524570924746	35.0	34.0	36.0	29.0	37.0
86-87	33.32220866401666	35.0	34.0	35.5	29.0	37.0
88-89	33.1316530883188	35.0	34.0	35.0	29.0	36.0
90-91	33.04702375192856	35.0	34.0	35.0	29.0	36.0
92-93	32.94954553967496	35.0	34.0	35.0	29.0	36.0
94-95	32.878657280406145	35.0	34.0	35.0	29.0	36.0
96-97	32.82546525056803	35.0	34.0	35.0	29.0	35.5
98-99	32.76053466029267	35.0	34.0	35.0	29.0	35.0
100	32.046315256145014	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31602112154300244
1101	2	0.30340939604629114
1101	3	0.3071391267548407
1101	4	0.20569805148262077
1101	5	0.1696304489653997
1101	6	0.24208726205309006
1101	7	0.22567550015131133
1101	8	0.24330077038296594
1101	9	0.2547397796868509
1101	10-11	0.2497182393616768
1101	12-13	0.32101990331923247
1101	14-15	0.4672701253998568
1101	16-17	0.47328653573499224
1101	18-19	0.41856542706312894
1101	20-21	0.38534203376125475
1101	22-23	0.39290001300884114
1101	24-25	0.34929870028096843
1101	26-27	0.37559050986077835
1101	28-29	0.41388039822707867
1101	30-31	0.45216488200314586
1101	32-33	0.47146195327584195
1101	34-35	0.4960712090040289
1101	36-37	0.5237368757820136
1101	38-39	0.49463297682150653
1101	40-41	0.5213956329026104
1101	42-43	0.4950512664060369
1101	44-45	0.4937220402067979
1101	46-47	0.5563825700360283
1101	48-49	0.6599697485943281
1101	50-51	0.6038933227925014
1101	52-53	0.5220370848504032
1101	54-55	0.5255134736139695
1101	56-57	0.4865410656957039
1101	58-59	0.4953047632296972
1101	60-61	0.5095867173239697
1101	62-63	0.5504594490464996
1101	64-65	0.4591781464638345
1101	66-67	0.4855006770646213
1101	68-69	0.42056962459838587
1101	70-71	0.43095586005555475
1101	72-73	0.4143018080893981
1101	74-75	0.41001547632694724
1101	76-77	0.36990872400110675
1101	78-79	0.3823362094146816
1101	80-81	0.2992053149012719
1101	82-83	0.2945978021371758
1101	84-85	0.27883259602535304
1101	86-87	0.3616721738289925
1101	88-89	0.401687145774865
1101	90-91	0.39865510717709185
1101	92-93	0.353963060113756
1101	94-95	0.38835649630114233
1101	96-97	0.3614593516866478
1101	98-99	0.38973526024656735
1101	100	0.3735538628797386
1102	1	0.251736005377289
1102	2	0.2168301282294891
1102	3	0.24214163361593677
1102	4	0.1639520657933815
1102	5	0.17801823993018218
1102	6	0.19365224892558075
1102	7	0.19620832535156296
1102	8	0.2259949951951512
1102	9	0.25565171688455024
1102	10-11	0.25567934084664046
1102	12-13	0.2543872356909702
1102	14-15	0.40880958528101985
1102	16-17	0.49416937027512375
1102	18-19	0.4020265551371409
1102	20-21	0.32394537283732916
1102	22-23	0.3589065121230135
1102	24-25	0.3257457103903505
1102	26-27	0.3687999346998083
1102	28-29	0.3629420742004186
1102	30-31	0.4226724213860251
1102	32-33	0.4053106599017866
1102	34-35	0.32859958551823354
1102	36-37	0.3451859148984795
1102	38-39	0.3725230161718045
1102	40-41	0.3649001662991367
1102	42-43	0.32153829491655017
1102	44-45	0.3560235079676133
1102	46-47	0.4018612696559529
1102	48-49	0.5301198561714031
1102	50-51	0.49989079361156996
1102	52-53	0.42358300999150345
1102	54-55	0.43977308675594884
1102	56-57	0.40227703802150927
1102	58-59	0.396589037084091
1102	60-61	0.36033120339047287
1102	62-63	0.39371665179695725
1102	64-65	0.3942405573436858
1102	66-67	0.3060131372952597
1102	68-69	0.31362126773461085
1102	70-71	0.31613769698929417
1102	72-73	0.34479908035188345
1102	74-75	0.3529219881589256
1102	76-77	0.3523571610814429
1102	78-79	0.34159428638332656
1102	80-81	0.2994905892531392
1102	82-83	0.2120055894199382
1102	84-85	0.2336082076148287
1102	86-87	0.31263509460454486
1102	88-89	0.3677730825013015
1102	90-91	0.30001609232931514
1102	92-93	0.29693092900019735
1102	94-95	0.29945241628171715
1102	96-97	0.2518183548681989
1102	98-99	0.22960912176687032
1102	100	0.2819961351939071
1103	1	0.22882921201153295
1103	2	0.2274577140894607
1103	3	0.24074052677877944
1103	4	0.1670656397255854
1103	5	0.09912544033022641
1103	6	0.14885429993286436
1103	7	0.1788283842513394
1103	8	0.1821110743327381
1103	9	0.22304551722599797
1103	10-11	0.2320331492274761
1103	12-13	0.244228967625169
1103	14-15	0.36084174307492844
1103	16-17	0.4338471231507057
1103	18-19	0.35334936499602776
1103	20-21	0.34795970234167584
1103	22-23	0.33175374340871144
1103	24-25	0.311530262613104
1103	26-27	0.30176581002805847
1103	28-29	0.30212923915655665
1103	30-31	0.32608847798444174
1103	32-33	0.33225736192374455
1103	34-35	0.3226712363629858
1103	36-37	0.3562792806934141
1103	38-39	0.2937737934982039
1103	40-41	0.24291276084878888
1103	42-43	0.2682091063691061
1103	44-45	0.26050340026543495
1103	46-47	0.2918174477550508
1103	48-49	0.3163078876309058
1103	50-51	0.2749121668647163
1103	52-53	0.25212978690721854
1103	54-55	0.2298175566436953
1103	56-57	0.191814018476137
1103	58-59	0.17896584882608835
1103	60-61	0.1615112785340358
1103	62-63	0.17657531423522954
1103	64-65	0.12907071832974992
1103	66-67	0.1230031461516532
1103	68-69	0.12544792145605754
1103	70-71	0.14068892742522365
1103	72-73	0.11043157681775284
1103	74-75	0.11550825892759065
1103	76-77	0.11881840465012772
1103	78-79	0.14773955357563295
1103	80-81	0.11359658397367411
1103	82-83	0.09667392843886091
1103	84-85	0.06380371286638109
1103	86-87	0.04531198842488493
1103	88-89	0.16899170169003241
1103	90-91	0.12315063215953614
1103	92-93	0.11623756244899397
1103	94-95	0.09303524670430363
1103	96-97	0.08965928847802473
1103	98-99	0.08252574723012884
1103	100	0.08840880438521737
1104	1	0.1940116843297588
1104	2	0.14706397916908287
1104	3	0.1257762481982354
1104	4	0.11673039964436782
1104	5	0.08353686917937608
1104	6	0.1226348728551514
1104	7	0.16093926018972837
1104	8	0.1418433344997041
1104	9	0.18671372086375015
1104	10-11	0.18711278727192848
1104	12-13	0.2013845137268362
1104	14-15	0.32670016592748397
1104	16-17	0.40181798677313196
1104	18-19	0.31334239408587194
1104	20-21	0.24436581512264866
1104	22-23	0.2654053790117672
1104	24-25	0.2572290165343105
1104	26-27	0.25531268147115327
1104	28-29	0.23367441380362663
1104	30-31	0.3054837692365169
1104	32-33	0.3003107987393747
1104	34-35	0.31526723239947074
1104	36-37	0.2749181372026186
1104	38-39	0.2988735258967665
1104	40-41	0.29496377163076204
1104	42-43	0.2642510008090184
1104	44-45	0.282222874364372
1104	46-47	0.3887274695090426
1104	48-49	0.5070874069072602
1104	50-51	0.413869365781494
1104	52-53	0.3602902359659055
1104	54-55	0.37996706016385673
1104	56-57	0.33914585820906495
1104	58-59	0.2866879461971834
1104	60-61	0.26796882915649434
1104	62-63	0.3269263333971182
1104	64-65	0.23171261645290286
1104	66-67	0.27290081641616126
1104	68-69	0.2602455945211304
1104	70-71	0.33508812748685557
1104	72-73	0.24512335677759722
1104	74-75	0.271486214854491
1104	76-77	0.19890666506860555
1104	78-79	0.24012826392674924
1104	80-81	0.1911816180169339
1104	82-83	0.20575616785220063
1104	84-85	0.2420808715465128
1104	86-87	0.26226612022837514
1104	88-89	0.20318568053233577
1104	90-91	0.16564027468599818
1104	92-93	0.183703729778955
1104	94-95	0.12886556960771856
1104	96-97	0.1492880447410272
1104	98-99	0.11967269166238026
1104	100	0.1507120630596006
1105	1	0.1832055269984849
1105	2	0.16175008758662557
1105	3	0.161541259532882
1105	4	0.10385764883037751
1105	5	0.07808442202055232
1105	6	0.10234460261434464
1105	7	0.14406348508505573
1105	8	0.0955878406113655
1105	9	0.17256223584658414
1105	10-11	0.1470289995293328
1105	12-13	0.181745546677611
1105	14-15	0.2803655019908078
1105	16-17	0.357146744588114
1105	18-19	0.2693664742580353
1105	20-21	0.24365454790226693
1105	22-23	0.26063902500878555
1105	24-25	0.26104348669488786
1105	26-27	0.2279222036042512
1105	28-29	0.25719200427467115
1105	30-31	0.271526856407462
1105	32-33	0.3173000955984904
1105	34-35	0.3154477045334403
1105	36-37	0.26054174840761846
1105	38-39	0.2766143808538857
1105	40-41	0.28218116020916284
1105	42-43	0.28942967995149615
1105	44-45	0.29426395297236496
1105	46-47	0.3368581850077774
1105	48-49	0.38767266562472
1105	50-51	0.30353724137042093
1105	52-53	0.29548783703351944
1105	54-55	0.2844064142826923
1105	56-57	0.28759273830333143
1105	58-59	0.27179619158112445
1105	60-61	0.21866454079004427
1105	62-63	0.22826882642919344
1105	64-65	0.2168356337149575
1105	66-67	0.2271556612699257
1105	68-69	0.1951529268947283
1105	70-71	0.19884093420814963
1105	72-73	0.20587378056035277
1105	74-75	0.20201442009295079
1105	76-77	0.11822806767415273
1105	78-79	0.16456975932341322
1105	80-81	0.17132345743594612
1105	82-83	0.158322287990309
1105	84-85	0.2082466065536579
1105	86-87	0.21496581887690525
1105	88-89	0.2915244505870902
1105	90-91	0.23031842742886255
1105	92-93	0.24402959225426457
1105	94-95	0.22067957222417078
1105	96-97	0.23127280614374257
1105	98-99	0.22372653585417623
1105	100	0.22781812168998528
1106	1	0.09455208767822398
1106	2	0.07031203096052252
1106	3	0.10184005561932707
1106	4	0.08699620707682243
1106	5	0.07441073613108529
1106	6	0.0780568305530025
1106	7	0.055502333027448
1106	8	0.0768994968240051
1106	9	0.1124508580783683
1106	10-11	0.10624512655537899
1106	12-13	0.09473621532751508
1106	14-15	0.17809927104266876
1106	16-17	0.23896637640347507
1106	18-19	0.1615807008885497
1106	20-21	0.13161041718176136
1106	22-23	0.12182578925464327
1106	24-25	0.12921602826681067
1106	26-27	0.17386655923168348
1106	28-29	0.16224086187359887
1106	30-31	0.20300133831166534
1106	32-33	0.16231255264793987
1106	34-35	0.11492042823175552
1106	36-37	0.12037028005840966
1106	38-39	0.1044458032820117
1106	40-41	0.13124275800039698
1106	42-43	0.1342041857188434
1106	44-45	0.10612530199354353
1106	46-47	0.16196785031807082
1106	48-49	0.24254378616568317
1106	50-51	0.16175095611820467
1106	52-53	0.1341839950692787
1106	54-55	0.14208108189893665
1106	56-57	0.07526248492138166
1106	58-59	0.1374338750521389
1106	60-61	0.17619569577799865
1106	62-63	0.13441260612673744
1106	64-65	0.1785964361096788
1106	66-67	0.16117474435690582
1106	68-69	0.11691695637620825
1106	70-71	0.10449207005613204
1106	72-73	0.11538426488301923
1106	74-75	0.09553120279873184
1106	76-77	0.07570321230575416
1106	78-79	0.059251928370827045
1106	80-81	0.09546573857825535
1106	82-83	0.08184991420216647
1106	84-85	0.09229009680253597
1106	86-87	0.094574883899611
1106	88-89	0.12645547624428133
1106	90-91	0.09103616367077905
1106	92-93	0.05201220555159125
1106	94-95	0.09139547854715602
1106	96-97	0.1030661266923687
1106	98-99	0.10633880694430076
1106	100	0.028316898240809962
1107	1	0.13874920282758296
1107	2	0.09998456289812907
1107	3	0.094836792839601
1107	4	0.04877017817756979
1107	5	0.031451266246705245
1107	6	0.06765249596735146
1107	7	0.07207974294458097
1107	8	0.07166111938182951
1107	9	0.04277619968554802
1107	10-11	0.06936829724509153
1107	12-13	0.08620227933744218
1107	14-15	0.21596366965153635
1107	16-17	0.23973111494311894
1107	18-19	0.16728549695122297
1107	20-21	0.12442506459191804
1107	22-23	0.1616321489264223
1107	24-25	0.14800364673966726
1107	26-27	0.15734119913884115
1107	28-29	0.17127238838450864
1107	30-31	0.19391617921716886
1107	32-33	0.10097142392309877
1107	34-35	0.12886864542258536
1107	36-37	0.10359105020974368
1107	38-39	0.15358820751752944
1107	40-41	0.11995936374292171
1107	42-43	0.20198818378086258
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2216	52-53	-0.4575266643970437
2216	54-55	-0.4848027786578797
2216	56-57	-0.5688478750249786
2216	58-59	-0.5376429204713133
2216	60-61	-0.5693212663776137
2216	62-63	-0.5405009142582244
2216	64-65	-0.4459783152094232
2216	66-67	-0.46916337652300655
2216	68-69	-0.44991816446693633
2216	70-71	-0.4237766929922344
2216	72-73	-0.3756235868739495
2216	74-75	-0.37656643335417783
2216	76-77	-0.3591372081831139
2216	78-79	-0.36360102822780505
2216	80-81	-0.38346132018608614
2216	82-83	-0.4258163358196043
2216	84-85	-0.41981338269833657
2216	86-87	-0.3631129303980174
2216	88-89	-0.24163788962088262
2216	90-91	-0.3171755590139895
2216	92-93	-0.311133605329033
2216	94-95	-0.28990062246924
2216	96-97	-0.3164459924500136
2216	98-99	-0.3195087397202556
2216	100	-0.3840189510859453
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	40.0
8	149.0
9	287.0
10	313.0
11	239.0
12	235.0
13	339.0
14	633.0
15	1063.0
16	1950.0
17	3653.0
18	6115.0
19	9605.0
20	13769.0
21	20535.0
22	28939.0
23	33046.0
24	31644.0
25	26856.0
26	24910.0
27	27219.0
28	33900.0
29	41963.0
30	51453.0
31	65216.0
32	83642.0
33	110470.0
34	160043.0
35	248528.0
36	443681.0
37	1018186.0
38	1345053.0
39	495404.0
40	1221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02570320077594	20.492817883700525	13.507020460948684	23.974458454574847
2	15.030918111805876	21.29255166336116	40.1722065850867	23.504323639746264
3	17.44345764975773	27.000868763183284	31.307666407869768	24.24800717918922
4	11.971496612675416	18.15313003763591	39.91239004567187	29.9629833040168
5	12.314679374938512	38.86233377780877	35.67954629326419	13.143440553988524
6	28.593579103727574	39.77656928966272	18.327124102101745	13.302727504507967
7	25.166945746702478	33.93654803051706	23.61959301193751	17.276913210842945
8	25.412178698754488	35.35042290548283	20.912702683104705	18.324695712657974
9	26.295406784749325	14.717525344664342	21.334156986813845	37.65291088377249
10-11	24.213301741970383	26.375312550696574	29.22324620883438	20.188139498498657
12-13	25.198132878060903	24.532613321799108	28.899827055902115	21.36942674423787
14-15	22.51652945141689	25.419456984629935	27.042877619823656	25.02113594412952
16-17	21.242841409818794	28.260701888812452	27.862656678414154	22.6338000229546
18-19	21.041374899559372	27.305002003905276	30.418981896035014	21.234641200500334
20-21	22.169625050689422	26.37938734847442	30.16533296137997	21.285654639456183
22-23	22.102054799423872	26.2010423755762	29.935771208514144	21.76113161648578
24-25	21.70774040880761	26.507695423482108	30.3502343139657	21.434329853744575
26-27	21.67479795519445	26.77322151324987	29.872275283211437	21.67970524834425
28-29	21.490411405581273	26.877611972008413	29.948518590278134	21.683458032132176
30-31	22.004461821245645	26.548275180821996	29.848835916356265	21.59842708157609
32-33	21.979155444847073	26.91830330590908	29.335184478920183	21.767356770323662
34-35	22.158909903744465	26.732372713970047	29.828684941088905	21.280032441196578
36-37	22.170512936866483	26.354739014557655	29.75545106700484	21.719296981571016
38-39	22.02606324379667	26.50736113842206	29.862541046762743	21.604034571018534
40-41	21.916407916950376	26.268495201867502	30.03045877996069	21.784638101221436
42-43	22.07716045605144	26.302536474947125	29.50568563247682	22.114617436524618
44-45	21.917168568796935	26.898790412113406	29.326672326813313	21.857368692276346
46-47	22.260092312289608	26.70571860939921	28.750969043491438	22.28322003481975
48-49	22.577658330020437	26.683483829117094	28.479895785475296	22.258962055387173
50-51	22.28205383413301	26.928162730489174	28.17432321679255	22.615460218585266
52-53	22.114374510857605	27.123977809302534	28.177176872735572	22.584470807104292
54-55	22.08450617707325	27.097441809284234	28.07382051120399	22.744231502438524
56-57	22.448086972355583	26.878165704857786	27.995918523343267	22.677828799443365
58-59	21.973320598175242	26.876444988008252	28.463367002908246	22.68686741090826
60-61	22.013540253288852	27.30984544047853	28.16505701083315	22.51155729539947
62-63	22.221011116221106	26.875430037248055	28.087437953108722	22.81612089342212
64-65	21.70236311154008	26.97234928912902	28.203029984274714	23.122257615056185
66-67	22.00197471072428	26.928936855789885	28.028779832686507	23.04030860079933
68-69	21.79536149898203	26.788350262528777	28.199246130425625	23.217042108063566
70-71	21.9203610402354	26.592018829813117	28.155311779291015	23.332308350660465
72-73	22.248187312608525	26.58363765142871	28.22507322455708	22.943101811405686
74-75	22.03029568930857	26.9709945552005	27.9285629004013	23.07014685508963
76-77	22.06547274355402	26.875094180360946	27.75690526076341	23.30252781532162
78-79	21.803819432617118	26.971384218996107	27.767261963251315	23.457534385135457
80-81	21.81141609970595	27.070415978004057	27.868937450075087	23.2492304722149
82-83	22.101941136050645	26.602084140874705	28.256009695461497	23.039965027613153
84-85	22.23435897909604	26.833507302068227	27.62567316663126	23.306460552204477
86-87	21.61639571937378	27.14429089340441	27.777607145554086	23.461706241667727
88-89	21.964985805836594	26.638587017392094	27.621174601950305	23.775252574821014
90-91	21.804898044015424	26.637080571784864	27.845461053506686	23.712560330693027
92-93	21.99838717760006	26.754642926339695	27.561527534947373	23.685442361112873
94-95	21.35794779990943	26.667708165186742	27.854035021600126	24.1203090133037
96-97	21.54491554084676	26.646134547386392	27.650940578208587	24.15800933355826
98-99	21.291169948485063	26.973285322775165	27.520014502467788	24.215530226271984
100	21.65684597659073	26.643177434067105	27.25203941957284	24.44793716976933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	127.0
2	279.0
3	494.0
4	329.5
5	247.5
6	574.0
7	1562.0
8	2912.5
9	3495.5
10	2702.0
11	1830.0
12	1793.5
13	2088.5
14	2458.0
15	2916.0
16	3491.0
17	4186.0
18	5051.0
19	5935.0
20	6825.0
21	8061.0
22	9791.5
23	11806.5
24	14013.0
25	17031.0
26	21934.0
27	28346.0
28	34725.5
29	42235.0
30	52276.5
31	62505.5
32	75838.0
33	86863.5
34	94587.5
35	102992.5
36	112140.0
37	127397.5
38	136032.0
39	135039.5
40	134502.5
41	133134.5
42	134521.0
43	139716.0
44	146252.0
45	159314.0
46	169143.5
47	176904.5
48	185197.5
49	180949.0
50	170005.5
51	163906.0
52	158601.0
53	152208.5
54	145388.5
55	130421.0
56	116975.0
57	102237.0
58	88010.0
59	79343.5
60	64356.0
61	48785.5
62	37366.0
63	27762.5
64	18936.0
65	12289.0
66	8631.0
67	6232.0
68	4627.5
69	3390.0
70	2381.5
71	1692.0
72	1245.0
73	954.5
74	692.0
75	497.5
76	316.5
77	167.0
78	96.5
79	51.0
80	28.5
81	20.0
82	16.0
83	12.0
84	6.0
85	1.5
86	1.5
87	2.5
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002332401373484199
2	0.003533241684584975
3	1.6165158034048903E-4
4	0.052051808869637464
5	0.0023785875392957675
6	0.004364592669193204
7	4.6186165811568294E-5
8	4.387685752098988E-4
9	2.3093082905784147E-5
10-11	8.082579017024452E-5
12-13	0.0
14-15	0.0
16-17	3.2330316068097806E-4
18-19	1.5010503888759696E-4
20-21	2.0783774615205735E-4
22-23	0.0
24-25	0.0
26-27	2.655704534165177E-4
28-29	0.0
30-31	1.5010503888759696E-4
32-33	2.193842876049494E-4
34-35	1.0391887307602867E-4
36-37	4.6186165811568294E-5
38-39	6.119666970032799E-4
40-41	5.195943653801433E-4
42-43	0.0
44-45	5.657805311917116E-4
46-47	0.0
48-49	5.773270726446037E-5
50-51	0.0
52-53	4.156754923041147E-4
54-55	3.2330316068097806E-4
56-57	1.2701195598181282E-4
58-59	7.274321115322007E-4
60-61	6.350597799090641E-4
62-63	1.731981217933811E-4
64-65	3.348497021338701E-4
66-67	0.0010738283551189629
68-69	0.002517146036730472
70-71	0.0033831366456973776
72-73	0.002955914611940371
74-75	0.0026326114512593927
76-77	0.00412211529868247
78-79	0.0011662006867420994
80-81	0.001108467979477639
82-83	6.350597799090641E-4
84-85	4.8495474102146707E-4
86-87	0.0
88-89	0.0
90-91	2.3093082905784147E-5
92-93	9.237233162313659E-5
94-95	4.6186165811568294E-5
96-97	2.886635363223018E-4
98-99	8.082579017024452E-5
100	0.07417498229337868
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4330301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.461385632434826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.1224991972253	28.95436550155761
2	15.60129490640276	13.873103783978285
3	7.056271782235784	9.411948625117601
4	3.7942559382406067	6.747915058330856
5	2.279541999940056	5.0675797962333275
6	1.4939862976897726	3.985482054669513
7	1.003319589217786	3.1226285418151654
8	0.7094823350897503	2.5235654159860585
9	0.5311526204107413	2.1254203337184214
>10	2.294485681600515	16.663945671202818
>50	0.07881711156920862	2.3211559165497673
>100	0.03175961430226849	2.5029626355391263
>500	0.0018191183664192179	0.5299471614586673
>1k	0.0010106213146771892	0.6722519486673031
>5k	1.0106213146771892E-4	0.3354295031616481
>10k+	2.0212426293543783E-4	1.162298052013711
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13612	0.31434304451353384	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12255	0.28300573101038473	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7465	0.17238986389167865	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1546541452892074E-4
2	0.0	0.0	0.0	0.0	1.1546541452892074E-4
3	0.0	0.0	0.0	0.0	1.1546541452892074E-4
4	0.0	0.0	0.0	0.0	1.1546541452892074E-4
5	0.0	0.0	0.0	0.0	1.1546541452892074E-4
6	0.0	0.0	0.0	0.0	1.385584974347049E-4
7	0.0	0.0	0.0	0.0	1.385584974347049E-4
8	0.0	0.0	0.0	0.0	1.385584974347049E-4
9	0.0	0.0	0.0	0.0	1.385584974347049E-4
10-11	0.0	0.0	0.0	0.0	1.385584974347049E-4
12-13	0.0	0.0	0.0	0.0	2.655704534165177E-4
14-15	0.0	0.0	0.0	0.0	3.2330316068097806E-4
16-17	0.0	0.0	0.0	1.1546541452892073E-5	3.925824093983305E-4
18-19	0.0	0.0	0.0	3.463962435867622E-5	4.387685752098988E-4
20-21	0.0	0.0	0.0	9.23723316231366E-5	5.311409068330354E-4
22-23	0.0	0.0	0.0	2.0783774615205735E-4	5.542339897388196E-4
24-25	0.0	0.0	0.0	5.888736140974958E-4	5.542339897388196E-4
26-27	0.0	0.0	0.0	0.0011200145209305313	5.542339897388196E-4
28-29	0.0	0.0	0.0	0.004803361244403103	5.542339897388196E-4
30-31	0.0	0.0	0.0	0.01576102908319768	5.542339897388196E-4
32-33	0.0	0.0	0.0	0.0396277302663256	5.773270726446036E-4
34-35	0.0	0.0	0.0	0.07811235292881488	6.466063213619561E-4
36-37	0.0	0.0	0.0	0.13977088428725853	6.466063213619561E-4
38-39	0.0	0.0	0.0	0.23403684870866948	7.158855700793085E-4
40-41	0.0	0.0	0.0	0.33854459539879556	7.389786529850927E-4
42-43	0.0	0.0	0.0	0.44391833269788866	7.389786529850927E-4
44-45	0.0	0.0	0.0	0.5784355406240813	7.620717358908769E-4
46-47	0.0	0.0	0.0	0.7451329595794842	8.082579017024451E-4
48-49	0.0	0.0	0.0	0.9258247867757923	8.659906089669055E-4
50-51	1.1546541452892073E-5	0.0	0.0	1.1230397147911888	9.23723316231366E-4
52-53	2.3093082905784147E-5	0.0	0.0	1.3210513541668352	9.930025649487184E-4
54-55	2.3093082905784147E-5	0.0	0.0	1.536209607600026	9.930025649487184E-4
56-57	2.3093082905784147E-5	0.0	0.0	1.7984892967024693	9.930025649487184E-4
58-59	2.3093082905784147E-5	0.0	0.0	2.0907438074166205	9.930025649487184E-4
60-61	2.3093082905784147E-5	0.0	0.0	2.3997408032374654	9.930025649487184E-4
62-63	2.3093082905784147E-5	0.0	0.0	2.713471881053996	0.0010160956478545025
64-65	2.3093082905784147E-5	0.0	0.0	3.040954889740921	0.0010276421893073945
66-67	2.3093082905784147E-5	0.0	0.0	3.414947367400095	0.0010391887307602866
68-69	2.3093082905784147E-5	0.0	0.0	3.8336480535648674	0.0010391887307602866
70-71	2.3093082905784147E-5	0.0	0.0	4.288119925150699	0.0010507352722131788
72-73	2.3093082905784147E-5	0.0	0.0	4.747568356102728	0.0011431076038363153
74-75	2.3093082905784147E-5	0.0	0.0	5.202455903180864	0.0012123868525536676
76-77	2.3093082905784147E-5	0.0	0.0	5.71239735990639	0.001247026476912344
78-79	2.3093082905784147E-5	0.0	0.0	6.271180686977649	0.00128166610127102
80-81	2.3093082905784147E-5	0.0	0.0	6.866612736620388	0.0013163057256296963
82-83	2.3093082905784147E-5	0.0	0.0	7.4889597743898175	0.001420224598705725
84-85	2.3093082905784147E-5	0.0	0.0	8.103512896678545	0.0014433176816115094
86-87	2.3093082905784147E-5	0.0	0.0	8.74048940246879	0.0015934227204991063
88	2.3093082905784147E-5	0.0	0.0	9.246331836978538	0.001708888135028027
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7760	0.0	61.881508	1
GTATCAA	15090	0.0	44.009083	1
CAACGCA	19480	0.0	33.489326	5
ATCAACG	19525	0.0	33.432217	3
TATCAAC	19915	0.0	33.15688	2
TCAACGC	20660	0.0	31.617949	4
AACGCAG	21040	0.0	31.40729	6
ACGCAGA	22535	0.0	28.926422	7
CGCAGAG	22680	0.0	28.617155	8
GCAGAGT	24905	0.0	25.928417	9
GTGGTAT	2490	0.0	25.877249	1
GTACATG	14500	0.0	23.808111	1
TACATGG	14175	0.0	23.689833	2
GTACTAG	2190	0.0	23.408806	1
GAGTACT	17180	0.0	23.252604	12-13
ACATGGG	14035	0.0	23.187847	3
AGAGTAC	23180	0.0	22.981792	10-11
CAGAGTA	24250	0.0	22.636377	10-11
TGGTATC	2845	0.0	21.325258	2
AGTACTT	18465	0.0	21.176289	12-13
>>END_MODULE