FastQCFastQC Report
Mon 16 Jan 2017
ERR1146623_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146623_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4169617
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.037.6235661
GTATCAA67800.035.376141
CAACGCA70200.031.4741025
ATCAACG72500.030.5419243
TATCAAC86400.027.4787082
AACGCAG84550.027.3469286
GTACATG144400.026.5762021
TATTCGC4550.025.8183389
TCAACGC88600.025.5756574
ACGCAGA86750.025.3526277
TACATGG147650.025.1861462
CGCAGAG90600.024.3784378
ACATGGG147950.024.149723
CATGGGG124850.021.5011184
GTACTAG19950.021.4520341
GCAGAGT104050.020.0963699
GCTATTG28300.020.0925528
TAGTACT28650.020.0192894
GTATAAG23900.019.8743741
ATGGGGG83050.019.4720365
Produced by FastQC (version 0.11.5)