##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146623_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4169617
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.250054381493552	31.0	31.0	34.0	28.0	34.0
2	31.43908349375974	33.0	31.0	34.0	28.0	34.0
3	31.44734732230802	33.0	31.0	34.0	28.0	34.0
4	34.93807776589553	37.0	35.0	37.0	32.0	37.0
5	35.0028266385138	37.0	35.0	37.0	32.0	37.0
6	35.005073367649835	37.0	35.0	37.0	32.0	37.0
7	34.96107052518253	37.0	35.0	37.0	32.0	37.0
8	34.949140172826425	37.0	35.0	37.0	32.0	37.0
9	36.52284778194256	39.0	37.0	39.0	32.0	39.0
10-11	36.518762154893366	39.0	37.0	39.0	32.0	39.0
12-13	36.478868682663176	39.0	37.0	39.0	32.0	39.0
14-15	37.810169015523485	40.0	38.0	41.0	32.0	41.0
16-17	37.82662100619793	40.0	38.0	41.0	32.0	41.0
18-19	37.8341537124393	40.0	38.0	41.0	32.0	41.0
20-21	37.93884390340887	40.0	38.0	41.0	32.0	41.0
22-23	37.94659617418098	40.0	38.0	41.0	32.0	41.0
24-25	37.95579785865225	40.0	38.0	41.0	32.0	41.0
26-27	38.01614584744834	40.0	38.0	41.0	32.0	41.0
28-29	37.99861761883645	40.0	38.0	41.0	32.0	41.0
30-31	37.96162213459893	40.0	38.0	41.0	32.0	41.0
32-33	37.9332306780215	40.0	38.0	41.0	32.0	41.0
34-35	37.88269522116779	40.0	38.0	41.0	31.5	41.0
36-37	37.810325144971344	40.0	38.0	41.0	31.0	41.0
38-39	37.75145031306232	40.0	38.0	41.0	31.0	41.0
40-41	37.700830795730155	40.0	38.0	41.0	31.0	41.0
42-43	37.540118432940005	40.0	37.0	41.0	30.5	41.0
44-45	37.40460718574392	40.0	37.0	41.0	30.0	41.0
46-47	37.293577803428946	40.0	37.0	41.0	30.0	41.0
48-49	37.18461347888787	40.0	36.0	41.0	30.0	41.0
50-51	37.04772956365057	40.0	36.0	41.0	30.0	41.0
52-53	36.91473113717639	39.5	35.5	41.0	30.0	41.0
54-55	36.794557509718516	39.0	35.0	41.0	30.0	41.0
56-57	36.60303260467329	39.0	35.0	41.0	30.0	41.0
58-59	36.408994399245785	39.0	35.0	41.0	29.5	41.0
60-61	36.194683828274876	38.5	35.0	40.5	29.0	41.0
62-63	35.96207157635821	38.0	35.0	40.0	29.0	41.0
64-65	35.67157499118025	37.0	35.0	40.0	29.0	41.0
66-67	35.44403802555486	37.0	34.0	40.0	29.0	41.0
68-69	35.198320013564796	36.0	34.0	39.5	28.5	41.0
70-71	34.932610357258234	36.0	34.0	39.0	28.0	41.0
72-73	34.605670736664784	35.5	34.0	39.0	28.0	41.0
74-75	34.28198609128847	35.0	34.0	38.5	28.0	40.0
76-77	34.01889286234203	35.0	34.0	37.0	28.0	39.5
78-79	33.71364660111468	35.0	33.5	37.0	27.0	39.0
80-81	33.476637182743644	35.0	33.0	36.0	27.0	39.0
82-83	33.20709587954961	35.0	33.0	36.0	27.0	37.5
84-85	32.99754018174811	35.0	33.0	36.0	26.5	37.0
86-87	32.78829422462543	35.0	33.0	35.0	26.0	37.0
88-89	32.61620611677284	35.0	33.0	35.0	26.0	36.0
90-91	32.46522942035204	35.0	33.0	35.0	26.0	36.0
92-93	32.30902334674863	35.0	33.0	35.0	25.0	36.0
94-95	32.1870981195635	35.0	33.0	35.0	25.0	36.0
96-97	32.089802492651	35.0	33.0	35.0	25.0	35.0
98-99	31.98432457945178	35.0	33.0	35.0	25.0	35.0
100	30.828099079603714	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25367150198139754
1101	2	0.24298720494767068
1101	3	0.16752037125362662
1101	4	0.18235607723609348
1101	5	0.17440105652271853
1101	6	0.15156200884736393
1101	7	0.23957828326793873
1101	8	0.2753022612517597
1101	9	0.3604470764435277
1101	10-11	0.37547617553142487
1101	12-13	0.37660400302326025
1101	14-15	0.3922514458559121
1101	16-17	0.41300625340301167
1101	18-19	0.35516581938212255
1101	20-21	0.3750234729622477
1101	22-23	0.4095920366202961
1101	24-25	0.4259365870539824
1101	26-27	0.4214042934755682
1101	28-29	0.45318753686638047
1101	30-31	0.4473482845373198
1101	32-33	0.4316848978982364
1101	34-35	0.3986319642336511
1101	36-37	0.3909304317296858
1101	38-39	0.35250767906072866
1101	40-41	0.33480174993654543
1101	42-43	0.30943280757072245
1101	44-45	0.2656316081032557
1101	46-47	0.2525821001302049
1101	48-49	0.21836459438156908
1101	50-51	0.11997208702946693
1101	52-53	0.2160358309989121
1101	54-55	0.18261284625729246
1101	56-57	0.17264776151323957
1101	58-59	0.21792886200765338
1101	60-61	0.278991510516569
1101	62-63	0.2575440191407452
1101	64-65	0.26635138664714475
1101	66-67	0.14815684223241732
1101	68-69	0.22516648519934535
1101	70-71	0.18727371904159895
1101	72-73	0.24406577860749223
1101	74-75	0.31617941568386243
1101	76-77	0.20235281712874809
1101	78-79	0.21728901297288417
1101	80-81	0.20596115160920903
1101	82-83	0.2301170719293424
1101	84-85	0.1854355707622375
1101	86-87	0.1855252945584951
1101	88-89	0.14447882380582655
1101	90-91	0.1293549576425832
1101	92-93	0.15796455338168158
1101	94-95	0.13192231100134677
1101	96-97	0.15043229003894965
1101	98-99	0.22865680253373455
1101	100	0.24518127402028256
1102	1	0.22484026731402906
1102	2	0.2694059544384686
1102	3	0.23119358487297603
1102	4	0.2604298710186512
1102	5	0.15829217332682788
1102	6	0.2149068187101335
1102	7	0.2391931506541809
1102	8	0.2620147334531069
1102	9	0.38451006961494727
1102	10-11	0.3419899056247786
1102	12-13	0.3594903451485578
1102	14-15	0.3786506017627005
1102	16-17	0.33036945789947225
1102	18-19	0.3316159507939176
1102	20-21	0.3788179193680534
1102	22-23	0.3647383699143987
1102	24-25	0.40343263162435505
1102	26-27	0.32214720010529874
1102	28-29	0.4221609256008705
1102	30-31	0.42341896627937814
1102	32-33	0.42056221973290775
1102	34-35	0.3906092565974859
1102	36-37	0.3803935494229478
1102	38-39	0.42553602437853755
1102	40-41	0.3153390748088327
1102	42-43	0.39923614753248415
1102	44-45	0.36371937287564293
1102	46-47	0.39213956475261824
1102	48-49	0.3101668218944553
1102	50-51	0.28187071336634517
1102	52-53	0.22221998051364977
1102	54-55	0.244385089954001
1102	56-57	0.25644030689883834
1102	58-59	0.26021926949989194
1102	60-61	0.2904400275699288
1102	62-63	0.2831616746899641
1102	64-65	0.27021181555351603
1102	66-67	0.30356760844414765
1102	68-69	0.2799035459404635
1102	70-71	0.22940435031106432
1102	72-73	0.23835567714934314
1102	74-75	0.27430411456326453
1102	76-77	0.2316560378995547
1102	78-79	0.19431211590055852
1102	80-81	0.19764438933832906
1102	82-83	0.2528208859391583
1102	84-85	0.17911804808326792
1102	86-87	0.15191032860084164
1102	88-89	0.1348621760990696
1102	90-91	0.10956282263594375
1102	92-93	0.10521249972664037
1102	94-95	0.14863041789701725
1102	96-97	0.21332065388416765
1102	98-99	0.18948477227431937
1102	100	0.10345750130987952
1103	1	0.11845330302171675
1103	2	0.18138387709707615
1103	3	0.17218742828703526
1103	4	0.11238445648411499
1103	5	0.0839174495961359
1103	6	0.17010106390097945
1103	7	0.13944817827792377
1103	8	0.2029809977060566
1103	9	0.2776185276914376
1103	10-11	0.2638429036006045
1103	12-13	0.27974868800403385
1103	14-15	0.31559304730182447
1103	16-17	0.3224097171053728
1103	18-19	0.30794322180726397
1103	20-21	0.3498175079099042
1103	22-23	0.32068093073823434
1103	24-25	0.33919890237400807
1103	26-27	0.33010448871070963
1103	28-29	0.3248208178574359
1103	30-31	0.31475321806883727
1103	32-33	0.3616788158911888
1103	34-35	0.33795727822261057
1103	36-37	0.3274821485926438
1103	38-39	0.3784755776088673
1103	40-41	0.28567990037477387
1103	42-43	0.3190642268126993
1103	44-45	0.2566699991710024
1103	46-47	0.2957721160606894
1103	48-49	0.26783115356795406
1103	50-51	0.1964873662115849
1103	52-53	0.23282483673895626
1103	54-55	0.17580792944838208
1103	56-57	0.25316999481483293
1103	58-59	0.27194980772027577
1103	60-61	0.20440861789899856
1103	62-63	0.23488640727401133
1103	64-65	0.11210688608100838
1103	66-67	0.08298251940740187
1103	68-69	0.16498522611646393
1103	70-71	0.1183802016663904
1103	72-73	0.19659625724164442
1103	74-75	0.25539079720419267
1103	76-77	0.1619795962355326
1103	78-79	0.1533572368093843
1103	80-81	0.11182342204224227
1103	82-83	0.15007758742054023
1103	84-85	0.11096172455741282
1103	86-87	0.08471499234526192
1103	88-89	0.06578986455773617
1103	90-91	0.12195174167295875
1103	92-93	0.1094224618546562
1103	94-95	0.17013252996395778
1103	96-97	0.1820602798521378
1103	98-99	0.09246155418748003
1103	100	0.24817542317869012
1104	1	0.1574842306281674
1104	2	0.1931909994825851
1104	3	0.16499186758211337
1104	4	0.22166703469010685
1104	5	0.1925334128754983
1104	6	0.1889464480430263
1104	7	0.1976917518882928
1104	8	0.266456320480728
1104	9	0.2477843914636182
1104	10-11	0.24966139863078496
1104	12-13	0.2604012638262816
1104	14-15	0.35169280948244364
1104	16-17	0.4003690161757163
1104	18-19	0.39148767296558873
1104	20-21	0.3862110795800149
1104	22-23	0.3574961606025866
1104	24-25	0.4017044459492851
1104	26-27	0.34790837364825933
1104	28-29	0.38379752929770916
1104	30-31	0.37052782184179733
1104	32-33	0.40601321266704815
1104	34-35	0.30526231068024146
1104	36-37	0.37253998194168503
1104	38-39	0.34843251788793594
1104	40-41	0.31154864791481884
1104	42-43	0.32008424466301477
1104	44-45	0.22975178353701864
1104	46-47	0.2610804219995302
1104	48-49	0.2677299833192137
1104	50-51	0.281048716959134
1104	52-53	0.286404877650547
1104	54-55	0.2708168407456313
1104	56-57	0.3044809810723095
1104	58-59	0.3014825120426039
1104	60-61	0.315958518391831
1104	62-63	0.23472460417071517
1104	64-65	0.3463458086317175
1104	66-67	0.32199688558272754
1104	68-69	0.2951005602000265
1104	70-71	0.30864402041365935
1104	72-73	0.3076850015664121
1104	74-75	0.2531428774308111
1104	76-77	0.23458276217137097
1104	78-79	0.2675205865701784
1104	80-81	0.20570871992625683
1104	82-83	0.21792609813904562
1104	84-85	0.207924399665103
1104	86-87	0.20048875072298955
1104	88-89	0.2524617930012667
1104	90-91	0.18943610879068018
1104	92-93	0.21846805221770893
1104	94-95	0.22247531416920197
1104	96-97	0.26816863457443674
1104	98-99	0.2237344560828305
1104	100	0.2648158502557294
1105	1	0.08803980179953896
1105	2	0.05036026828684115
1105	3	0.07051228082902483
1105	4	0.09794602392352658
1105	5	0.037118074838332404
1105	6	0.1140832548273707
1105	7	0.1264131231639567
1105	8	0.17716575825693326
1105	9	0.28321221484870307
1105	10-11	0.25015071656344645
1105	12-13	0.21790212891836802
1105	14-15	0.3103197573594585
1105	16-17	0.2252211894314584
1105	18-19	0.25999139938413407
1105	20-21	0.271067907597633
1105	22-23	0.19732484137946216
1105	24-25	0.22367039872723637
1105	26-27	0.1602772890988362
1105	28-29	0.2020316292252886
1105	30-31	0.20280303721823145
1105	32-33	0.15557586656630207
1105	34-35	0.16045238315077626
1105	36-37	0.16462899992386326
1105	38-39	0.2593580016540855
1105	40-41	0.1628784622989059
1105	42-43	0.2510677852393428
1105	44-45	0.12012049424264148
1105	46-47	0.16022462445266683
1105	48-49	0.16300879608913021
1105	50-51	0.23706133249970662
1105	52-53	0.1465868117833864
1105	54-55	0.1553272859811301
1105	56-57	0.18822204715692692
1105	58-59	0.09146820959050217
1105	60-61	0.148250079922029
1105	62-63	0.08590283464565829
1105	64-65	0.21875783699076834
1105	66-67	0.1742116265326601
1105	68-69	0.17123420704509584
1105	70-71	0.19455768348898772
1105	72-73	0.1816510388715642
1105	74-75	0.15030086364117778
1105	76-77	0.059281333474288544
1105	78-79	0.06390237264204046
1105	80-81	0.051122149182397436
1105	82-83	0.0811903921802326
1105	84-85	0.11240448256864255
1105	86-87	0.08760652280046344
1105	88-89	0.11998006544978779
1105	90-91	0.10081321631657403
1105	92-93	0.1223774323701079
1105	94-95	0.13640860554452416
1105	96-97	0.13106953660825127
1105	98-99	0.1435478077999619
1105	100	0.16010948123205537
1106	1	0.12448923750178054
1106	2	0.12706878771579255
1106	3	0.1501300867079287
1106	4	0.135037421836536
1106	5	0.15824816967467115
1106	6	0.21349306340713525
1106	7	0.22591765154461996
1106	8	0.23981108723590694
1106	9	0.32293493874882984
1106	10-11	0.30099680435327514
1106	12-13	0.3477232614018533
1106	14-15	0.3921956717203088
1106	16-17	0.3569529591552225
1106	18-19	0.3804237277756499
1106	20-21	0.4502488838281735
1106	22-23	0.3746988128685871
1106	24-25	0.4311139121276497
1106	26-27	0.3591216193652329
1106	28-29	0.3442020417184608
1106	30-31	0.3866293474419962
1106	32-33	0.39937760860064486
1106	34-35	0.39038321207384286
1106	36-37	0.39755682340803844
1106	38-39	0.2993972434228951
1106	40-41	0.3073142704721903
1106	42-43	0.3458269011867614
1106	44-45	0.3514127894370418
1106	46-47	0.3408636602128041
1106	48-49	0.34727355044070407
1106	50-51	0.37893549076976285
1106	52-53	0.3780848842299278
1106	54-55	0.3675255515508198
1106	56-57	0.3413550643663683
1106	58-59	0.35140893284808783
1106	60-61	0.3547323168323331
1106	62-63	0.3401546530495594
1106	64-65	0.40743841303152095
1106	66-67	0.426717079602291
1106	68-69	0.40267947829953954
1106	70-71	0.38076838148712966
1106	72-73	0.3992621911665921
1106	74-75	0.3733282776399065
1106	76-77	0.3515144833713464
1106	78-79	0.36489959747309797
1106	80-81	0.3738606540129936
1106	82-83	0.3203411936246283
1106	84-85	0.31841984288036684
1106	86-87	0.27494354061833803
1106	88-89	0.2912197864710393
1106	90-91	0.2791685901871759
1106	92-93	0.265286524311108
1106	94-95	0.24159685922023755
1106	96-97	0.32908212067654574
1106	98-99	0.3433382615923932
1106	100	0.381630332440718
1107	1	0.08695881038720543
1107	2	0.1687854993802418
1107	3	0.14750634628848402
1107	4	0.14813386912113202
1107	5	0.14762266037681826
1107	6	0.218367338098993
1107	7	0.19557327989395645
1107	8	0.2067459274458301
1107	9	0.22238675381055373
1107	10-11	0.2735676693254092
1107	12-13	0.329237592873568
1107	14-15	0.30359520915494187
1107	16-17	0.25060012544548016
1107	18-19	0.27076738810914946
1107	20-21	0.29464849030241425
1107	22-23	0.3339504209160964
1107	24-25	0.3295271779834792
1107	26-27	0.29461302445722026
1107	28-29	0.2562004062120167
1107	30-31	0.2997770252529861
1107	32-33	0.3087447926110727
1107	34-35	0.26542174611194014
1107	36-37	0.311763265430983
1107	38-39	0.23742995982807003
1107	40-41	0.22455406176337078
1107	42-43	0.2838383042284818
1107	44-45	0.20016076662886206
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2216	68-69	-0.5196714466746997
2216	70-71	-0.4999469716427214
2216	72-73	-0.4400849639920352
2216	74-75	-0.39772390500446875
2216	76-77	-0.4325472009355451
2216	78-79	-0.3481213999140209
2216	80-81	-0.3864313265761936
2216	82-83	-0.32994523024700584
2216	84-85	-0.30375186984157665
2216	86-87	-0.3458442341205341
2216	88-89	-0.3253297535918165
2216	90-91	-0.3370287672146688
2216	92-93	-0.27740405637461407
2216	94-95	-0.36641410504288885
2216	96-97	-0.35604385724001375
2216	98-99	-0.32839003896387453
2216	100	-0.37617837820280187
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1289.0
1	313.0
2	251.0
3	201.0
4	179.0
5	260.0
6	434.0
7	1021.0
8	3016.0
9	4452.0
10	4649.0
11	5484.0
12	6713.0
13	8258.0
14	10133.0
15	10979.0
16	11768.0
17	13325.0
18	14411.0
19	15821.0
20	17598.0
21	19621.0
22	22037.0
23	25450.0
24	30727.0
25	37964.0
26	41781.0
27	40469.0
28	43359.0
29	53082.0
30	62006.0
31	70798.0
32	88070.0
33	117562.0
34	175848.0
35	267181.0
36	485791.0
37	970470.0
38	1119576.0
39	366704.0
40	566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83172589289465	20.51736575821775	13.317312943746588	24.333595405141008
2	14.80641978781016	20.798602519985344	40.691622055475385	23.70335563672911
3	17.42852993875795	27.0236459362185	30.517719150652084	25.03010497437147
4	12.19827040971977	17.767484864464862	40.30861297939195	29.72563174642341
5	12.93884562863992	38.77016290182085	35.638286764524096	12.652704705015136
6	29.06132227199186	39.422280153437704	18.63243123868781	12.883966335882628
7	25.842293149701106	33.33308529412329	23.692153255586643	17.132468300588958
8	25.23623397731722	35.62092719180603	21.19193709100886	17.950901739867895
9	26.185469770589854	15.68900446531967	21.21760063169693	36.90792513239355
10-11	24.20895504117556	26.73477835139576	29.037667127393235	20.018599480035444
12-13	24.68167124784871	24.720611095204507	28.45738444705297	22.140333209893818
14-15	22.31754892302323	25.918128379859855	25.83455333615517	25.929769360961743
16-17	21.964308216670965	28.58675419594477	26.798060594423006	22.650876992961255
18-19	21.87707133437048	28.568071660683465	27.993657598910097	21.56119940603596
20-21	23.197706246860466	27.637790910188375	27.155613625525337	22.00888921742582
22-23	23.309957712190883	27.440465489917194	26.710599361710656	22.538977436181266
24-25	22.782224311979288	27.648254042773857	27.272961835811582	22.296559809435276
26-27	22.64597760129948	27.85084461336525	26.88088867107108	22.622289114264188
28-29	22.381874712492902	28.129820413150924	26.972147170510112	22.51615770384606
30-31	22.737088533047274	27.790803024780402	26.915058360374307	22.557050081798018
32-33	22.93939559420714	27.99893422755802	26.420834891085377	22.64083528714946
34-35	23.035499573799637	27.943957112553143	26.96478848051589	22.055754833131335
36-37	22.969882226536104	27.687770096058962	26.86421041033597	22.478137267068966
38-39	22.81808315815778	27.65146414116209	27.184377341225176	22.346075359454957
40-41	22.73718475652649	27.309965166546586	27.420947816071013	22.531902260855915
42-43	22.6781236834493	27.359060809488888	27.086490554442634	22.87632495261918
44-45	22.524612547268116	27.932987714670404	27.034842480417627	22.507557257643853
46-47	22.649356921557658	27.66720747618146	26.86693901574589	22.816496586515
48-49	22.843820773355777	27.36691788382235	27.111959757882804	22.67730158493907
50-51	22.6879599819527	27.46044771846791	26.843519626436773	23.008072673142618
52-53	22.489678942305545	27.606779765181383	26.907946272163834	22.995595020349235
54-55	22.439319413886793	27.60730371865241	26.881833613568222	23.071543253892575
56-57	22.840844901025147	27.44579171120857	26.849844426418297	22.86351896134799
58-59	22.533425761708845	27.350012453823325	27.266310757402522	22.850251027065312
60-61	22.484534333916745	27.664459655068562	26.94764402242118	22.903361988593513
62-63	22.637701562129763	27.38198440823133	26.816853015182446	23.163461014456466
64-65	22.218822454710836	27.400516287173787	26.962470447931043	23.41819081018434
66-67	22.56624951563392	27.2183726356093	26.852667242544126	23.36271060621266
68-69	22.24339151233638	27.205900182048957	27.09951089496882	23.451197410645843
70-71	22.307808100157565	26.990041718714025	27.11736818463694	23.58478199649147
72-73	22.549678636145824	27.11892239156551	27.18548307330054	23.145915898988136
74-75	22.448208630466425	27.328753984482457	27.127420129688197	23.09561725536292
76-77	22.289752765548602	27.169393363067474	27.099831515082258	23.441022356301666
78-79	22.261127904926184	27.10859883480628	27.26674787400945	23.36352538625808
80-81	22.140311941961873	27.220244488822072	27.37298398035946	23.266459588856595
82-83	22.42142324397578	26.718912117848298	27.84682467950522	23.012839958670696
84-85	22.649468152342113	26.956395226353514	27.149757271093517	23.24437935021086
86-87	21.935627219044296	27.174990511031094	27.511347750484173	23.378034519440444
88-89	22.408476347917354	26.62698845208149	27.377679977990248	23.586855222010904
90-91	22.333875414483217	26.646433451736534	27.52732732386684	23.49236380991341
92-93	22.473682365864807	26.604456257465824	27.45902918345477	23.4628321932146
94-95	21.794251952512663	26.615699128445016	27.77047467650969	23.81957424253263
96-97	22.06853333826243	26.52448941232276	27.497125960123764	23.909851289291048
98-99	21.84850354606524	26.820173437882637	27.40254922956511	23.928773786487017
100	22.287009714998344	26.51852899051966	27.092576310233675	24.10188498424832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1760.0
1	1169.0
2	637.0
3	678.0
4	634.0
5	584.5
6	532.0
7	510.5
8	508.5
9	495.0
10	483.0
11	506.0
12	505.0
13	497.5
14	524.0
15	636.0
16	742.0
17	903.0
18	1190.5
19	1532.0
20	2112.0
21	2914.0
22	4257.5
23	6246.5
24	9004.0
25	12859.5
26	18863.0
27	25458.0
28	30825.5
29	39369.5
30	49099.0
31	57357.0
32	69376.0
33	80739.0
34	89481.5
35	98914.5
36	108939.0
37	122896.5
38	129424.0
39	126674.0
40	126315.5
41	126045.0
42	127577.0
43	134915.5
44	141944.5
45	152832.5
46	162946.5
47	169261.5
48	175632.0
49	171356.5
50	162514.5
51	161502.5
52	159133.5
53	153111.0
54	146855.5
55	133195.5
56	119929.0
57	104488.5
58	88230.5
59	78551.0
60	66019.5
61	52626.5
62	42217.0
63	32589.0
64	23267.5
65	15248.0
66	10552.0
67	7804.0
68	5806.0
69	4408.0
70	3402.5
71	2532.0
72	1927.5
73	1620.5
74	1241.0
75	862.0
76	614.5
77	410.0
78	267.5
79	183.0
80	127.5
81	95.0
82	74.0
83	64.0
84	51.5
85	41.0
86	33.0
87	26.0
88	24.5
89	25.0
90	20.0
91	18.5
92	16.5
93	14.0
94	14.5
95	12.5
96	12.5
97	14.5
98	14.0
99	10.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09813851008377988
2	0.06446635266500496
3	0.08063090686746528
4	0.08221378606236496
5	0.08365276714863738
6	0.0901041990187588
7	0.0865547123392868
8	0.08936072545751804
9	0.07343600143610313
10-11	0.08085874553945842
12-13	0.07036617511872194
14-15	0.07917993427214058
16-17	0.0813503974106015
18-19	0.08085874553945842
20-21	0.07185312224120344
22-23	0.07597820135518442
24-25	0.07826857958416804
26-27	0.07281244296538507
28-29	0.08191399833605821
30-31	0.08543950199742566
32-33	0.08692644911990718
34-35	0.08960055563856345
36-37	0.08964852167477252
38-39	0.09103953672483588
40-41	0.08755000759062523
42-43	0.09358173664391718
44-45	0.09003224996444517
46-47	0.08729818590052756
48-49	0.09667554597940291
50-51	0.09344983004434221
52-53	0.09842630630103437
54-55	0.09033203769075193
56-57	0.09746698557685274
58-59	0.10143617507315421
60-61	0.1002610071860317
62-63	0.10106443829253381
64-65	0.10311498634047205
66-67	0.105021636279783
68-69	0.10202375901671545
70-71	0.10408629857370592
72-73	0.1018079118537746
74-75	0.10542934758756019
76-77	0.10214367410723815
78-79	0.10932658802954803
80-81	0.10444604384527403
82-83	0.10872701257693453
84-85	0.10488972968020803
86-87	0.10202375901671545
88-89	0.10051282887612939
90-91	0.1004648628399203
92-93	0.09518859885692138
94-95	0.09494876867587598
96-97	0.0930061442094082
98-99	0.10641265132984637
100	0.24889576188892168
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4169617.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.26733943199877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.88442112839832	35.48138081262744
2	15.434284622519467	16.134179865106084
3	6.59012877546157	10.333454928228969
4	3.473429101767679	7.261875914202958
5	1.999844037733652	5.226326356564188
6	1.2130889744536297	3.8042959913389893
7	0.8022325605235714	2.9351393081000907
8	0.5646757690699762	2.361128007280432
9	0.41952643188943384	1.9734777374634318
>10	1.5836791367279341	12.887975360825573
>50	0.027016416845136892	0.9458083155456508
>100	0.00758984623134126	0.5988610060704846
>500	0.0	0.0
>1k	8.319837831375956E-5	0.056096396645770805
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	1.199150905227027E-5
14-15	0.0	0.0	0.0	0.0	2.398301810454054E-5
16-17	0.0	0.0	0.0	0.0	2.398301810454054E-5
18-19	0.0	0.0	0.0	3.597452715681081E-5	2.398301810454054E-5
20-21	0.0	0.0	0.0	8.394056336589187E-5	2.398301810454054E-5
22-23	0.0	0.0	0.0	1.9186414483632428E-4	2.398301810454054E-5
24-25	0.0	0.0	0.0	3.957197987249189E-4	2.398301810454054E-5
26-27	0.0	0.0	0.0	0.0010312697784952431	2.398301810454054E-5
28-29	0.0	0.0	0.0	0.003453554607053837	2.398301810454054E-5
30-31	0.0	0.0	0.0	0.01206345810658389	2.398301810454054E-5
32-33	0.0	0.0	0.0	0.03336037818341589	2.398301810454054E-5
34-35	0.0	0.0	0.0	0.06806380538068604	2.398301810454054E-5
36-37	0.0	0.0	0.0	0.12227741780599993	2.398301810454054E-5
38-39	0.0	0.0	0.0	0.20440726330499898	2.398301810454054E-5
40-41	0.0	0.0	0.0	0.2965260358445392	2.398301810454054E-5
42-43	0.0	0.0	0.0	0.3927698874980604	2.398301810454054E-5
44-45	0.0	0.0	0.0	0.5136682817630492	2.398301810454054E-5
46-47	0.0	0.0	0.0	0.6642216779143024	2.398301810454054E-5
48-49	0.0	0.0	0.0	0.8273901415885441	2.398301810454054E-5
50-51	0.0	0.0	0.0	1.004061044455642	2.398301810454054E-5
52-53	0.0	0.0	0.0	1.1793169492545719	2.398301810454054E-5
54-55	0.0	0.0	0.0	1.370641476183544	2.398301810454054E-5
56-57	1.199150905227027E-5	0.0	0.0	1.599235613246972	2.398301810454054E-5
58-59	2.398301810454054E-5	0.0	0.0	1.8589836908281985	2.398301810454054E-5
60-61	2.398301810454054E-5	0.0	0.0	2.134356704704533	2.398301810454054E-5
62-63	2.398301810454054E-5	0.0	0.0	2.4140586533487367	2.398301810454054E-5
64-65	2.398301810454054E-5	0.0	0.0	2.7083422770005017	2.398301810454054E-5
66-67	2.398301810454054E-5	0.0	0.0	3.0415863135630925	2.398301810454054E-5
68-69	2.398301810454054E-5	0.0	0.0	3.4153976252495135	2.398301810454054E-5
70-71	2.398301810454054E-5	0.0	0.0	3.8190797859851395	2.398301810454054E-5
72-73	2.398301810454054E-5	0.0	0.0	4.234333752956207	2.398301810454054E-5
74-75	2.398301810454054E-5	0.0	0.0	4.650559032160508	2.398301810454054E-5
76-77	2.398301810454054E-5	0.0	0.0	5.115829583388594	2.398301810454054E-5
78-79	2.398301810454054E-5	0.0	0.0	5.620276394690448	2.398301810454054E-5
80-81	2.398301810454054E-5	0.0	0.0	6.169919203610307	2.398301810454054E-5
82-83	2.398301810454054E-5	0.0	0.0	6.739156138321578	2.398301810454054E-5
84-85	2.398301810454054E-5	0.0	0.0	7.305802907077556	2.398301810454054E-5
86-87	2.398301810454054E-5	0.0	0.0	7.891660073335273	2.398301810454054E-5
88	2.398301810454054E-5	0.0	0.0	8.35350105297441	2.398301810454054E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4300	0.0	37.623566	1
GTATCAA	6780	0.0	35.37614	1
CAACGCA	7020	0.0	31.474102	5
ATCAACG	7250	0.0	30.541924	3
TATCAAC	8640	0.0	27.478708	2
AACGCAG	8455	0.0	27.346928	6
GTACATG	14440	0.0	26.576202	1
TATTCGC	455	0.0	25.818338	9
TCAACGC	8860	0.0	25.575657	4
ACGCAGA	8675	0.0	25.352627	7
TACATGG	14765	0.0	25.186146	2
CGCAGAG	9060	0.0	24.378437	8
ACATGGG	14795	0.0	24.14972	3
CATGGGG	12485	0.0	21.501118	4
GTACTAG	1995	0.0	21.452034	1
GCAGAGT	10405	0.0	20.096369	9
GCTATTG	2830	0.0	20.092552	8
TAGTACT	2865	0.0	20.019289	4
GTATAAG	2390	0.0	19.874374	1
ATGGGGG	8305	0.0	19.472036	5
>>END_MODULE