FastQCFastQC Report
Mon 16 Jan 2017
ERR1146623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4169617
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116020.2782509760488793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104460.25052660712003044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59310.1422432803780299No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77600.055.0243381
GTATCAA143150.044.1508031
ATCAACG177850.034.846643
CAACGCA177650.034.7112355
TATCAAC188850.033.44022
AACGCAG196550.031.8274086
TCAACGC195700.031.6922744
ACGCAGA209850.029.4952587
CGCAGAG214300.028.8389238
GCAGAGT232650.026.220879
GTGGTAT24400.025.0544091
GTACATG147100.024.1999191
TACATGG147800.023.861442
ACATGGG147300.023.3672583
GAGTACT152300.023.1133312-13
AGAGTAC217950.022.71751810-11
CAGAGTA227350.022.32605710-11
CATGGGG123450.021.2921814
AGTACTT166800.020.6391712-13
TGGTATC28700.020.4803772
Produced by FastQC (version 0.11.5)