##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4169617
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3291659641641	33.0	31.0	34.0	30.0	34.0
2	32.39277108664896	34.0	31.0	34.0	30.0	34.0
3	32.50182570725321	34.0	31.0	34.0	30.0	34.0
4	36.027703263872915	37.0	35.0	37.0	35.0	37.0
5	36.069245208852514	37.0	35.0	37.0	35.0	37.0
6	36.14333786532432	37.0	36.0	37.0	35.0	37.0
7	36.115988111138265	37.0	36.0	37.0	35.0	37.0
8	36.12372695142024	37.0	36.0	37.0	35.0	37.0
9	37.90892592772909	39.0	38.0	39.0	35.0	39.0
10-11	37.86211731197373	39.0	38.0	39.0	35.0	39.0
12-13	37.83548069283102	39.0	38.0	39.0	35.0	39.0
14-15	39.197636017888456	40.0	39.0	41.0	36.0	41.0
16-17	39.14761751498999	40.0	39.0	41.0	36.0	41.0
18-19	39.24909314212792	40.5	39.0	41.0	36.0	41.0
20-21	39.30477847245922	41.0	39.0	41.0	36.0	41.0
22-23	39.27297243367916	41.0	39.0	41.0	36.0	41.0
24-25	39.25894200834273	41.0	39.0	41.0	36.0	41.0
26-27	39.18183696488191	41.0	39.0	41.0	35.5	41.0
28-29	39.12118079430317	40.5	39.0	41.0	35.0	41.0
30-31	38.98238555243803	40.0	38.5	41.0	35.0	41.0
32-33	38.87665329933181	40.0	38.0	41.0	35.0	41.0
34-35	38.804421844980006	40.0	38.0	41.0	35.0	41.0
36-37	38.73388539043275	40.0	38.0	41.0	35.0	41.0
38-39	38.64382879770492	40.0	38.0	41.0	35.0	41.0
40-41	38.54469319364344	40.0	38.0	41.0	35.0	41.0
42-43	38.436427254589574	40.0	38.0	41.0	34.0	41.0
44-45	38.27771867296205	40.0	38.0	41.0	34.0	41.0
46-47	38.030828850707394	40.0	38.0	41.0	33.5	41.0
48-49	37.73605849170319	40.0	37.0	41.0	33.0	41.0
50-51	37.61825270762279	40.0	37.0	41.0	33.0	41.0
52-53	37.51017335644977	40.0	37.0	41.0	33.0	41.0
54-55	37.34345432686024	40.0	36.0	41.0	32.5	41.0
56-57	37.18049439552841	39.5	36.0	41.0	32.0	41.0
58-59	36.99247424883389	39.0	35.0	41.0	32.0	41.0
60-61	36.78627533416139	39.0	35.0	41.0	32.0	41.0
62-63	36.54438585126644	39.0	35.0	41.0	31.0	41.0
64-65	36.267646764678865	38.0	35.0	40.5	31.0	41.0
66-67	36.004179640480174	37.0	35.0	40.0	31.0	41.0
68-69	35.72239092943069	37.0	35.0	40.0	31.0	41.0
70-71	35.43466558199471	36.0	35.0	39.0	31.0	41.0
72-73	35.12691009749816	36.0	35.0	39.0	30.0	41.0
74-75	34.8221915825842	35.0	35.0	39.0	30.0	40.5
76-77	34.53938059538802	35.0	34.5	37.0	30.0	39.5
78-79	34.2620828243937	35.0	34.0	37.0	30.0	39.0
80-81	34.013794912098646	35.0	34.0	36.5	30.0	39.0
82-83	33.76411718390442	35.0	34.0	36.0	29.0	38.0
84-85	33.55242447927471	35.0	34.0	36.0	29.0	37.0
86-87	33.37055813519563	35.0	34.0	35.5	29.0	37.0
88-89	33.182639436667685	35.0	34.0	35.0	29.0	36.0
90-91	33.100097203172375	35.0	34.0	35.0	29.0	36.0
92-93	33.00115154461429	35.0	34.0	35.0	29.0	36.0
94-95	32.92782646463692	35.0	34.0	35.0	29.0	36.0
96-97	32.870291923694666	35.0	34.0	35.0	29.0	35.5
98-99	32.80497309465114	35.0	34.0	35.0	29.0	35.0
100	32.07967686240727	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27104988052357015
1101	2	0.25351493428861005
1101	3	0.2676159731444514
1101	4	0.22033247870677286
1101	5	0.1983369923845828
1101	6	0.18788974056642616
1101	7	0.18721932186370083
1101	8	0.20257582882041447
1101	9	0.15662205634115622
1101	10-11	0.20060232650597243
1101	12-13	0.27336928977516806
1101	14-15	0.38795461576977885
1101	16-17	0.399231450499677
1101	18-19	0.3602921034928386
1101	20-21	0.3456711266329009
1101	22-23	0.28767188803772825
1101	24-25	0.31596392206241575
1101	26-27	0.3411826148327748
1101	28-29	0.4051067796390626
1101	30-31	0.38533376025048227
1101	32-33	0.3450027352810423
1101	34-35	0.3529616528681956
1101	36-37	0.3827075672348741
1101	38-39	0.4297995849838756
1101	40-41	0.38327462037333504
1101	42-43	0.37316079358251386
1101	44-45	0.42420001802259577
1101	46-47	0.3684474600012919
1101	48-49	0.4367708776942649
1101	50-51	0.4693010674664606
1101	52-53	0.35819103515905937
1101	54-55	0.39004720504902224
1101	56-57	0.395577433624851
1101	58-59	0.4295114935251618
1101	60-61	0.3948619580239594
1101	62-63	0.41345857154692567
1101	64-65	0.40291920035289763
1101	66-67	0.335805403334966
1101	68-69	0.3483679530485517
1101	70-71	0.33044193833068647
1101	72-73	0.3190554245162147
1101	74-75	0.2709521108233446
1101	76-77	0.2888069583366857
1101	78-79	0.24096951838600944
1101	80-81	0.25505273707486964
1101	82-83	0.259333294647611
1101	84-85	0.21716312741036603
1101	86-87	0.24847263660182506
1101	88-89	0.30032293698889134
1101	90-91	0.20762771748206177
1101	92-93	0.20810061484331754
1101	94-95	0.25751479063724503
1101	96-97	0.2482056843043523
1101	98-99	0.3194932172144149
1101	100	0.2832948697744797
1102	1	0.23631884470895415
1102	2	0.21518322537202295
1102	3	0.2109607427739988
1102	4	0.14484165842158347
1102	5	0.1406410288841684
1102	6	0.17544771413923144
1102	7	0.18847842086059075
1102	8	0.20354323688496123
1102	9	0.26458290538305107
1102	10-11	0.2513891376501718
1102	12-13	0.29429489551162646
1102	14-15	0.41642304726716617
1102	16-17	0.42490875181540133
1102	18-19	0.360360097654997
1102	20-21	0.32546614837393406
1102	22-23	0.3536767180372351
1102	24-25	0.3643244178565368
1102	26-27	0.3323775217079259
1102	28-29	0.3359845279459961
1102	30-31	0.39294281996967584
1102	32-33	0.33040174333071093
1102	34-35	0.3159804226767804
1102	36-37	0.3179280906088664
1102	38-39	0.32581815463212394
1102	40-41	0.35949275691083926
1102	42-43	0.39670794642756135
1102	44-45	0.462723387998345
1102	46-47	0.42624556561955984
1102	48-49	0.4898889028863067
1102	50-51	0.43851826958714923
1102	52-53	0.3495363137389873
1102	54-55	0.37949961064426674
1102	56-57	0.37293572966700594
1102	58-59	0.3140535840237817
1102	60-61	0.342568060298845
1102	62-63	0.32320869300099275
1102	64-65	0.33883495397665797
1102	66-67	0.33993293061057983
1102	68-69	0.31266578759541375
1102	70-71	0.2922632162789469
1102	72-73	0.3153930436945984
1102	74-75	0.24648301346596213
1102	76-77	0.22645490347464658
1102	78-79	0.2451762045456789
1102	80-81	0.2440344641467007
1102	82-83	0.2328495181955148
1102	84-85	0.2423696496196115
1102	86-87	0.2181018270186712
1102	88-89	0.30735967898222327
1102	90-91	0.2677684030362144
1102	92-93	0.22910718995926516
1102	94-95	0.2657715551008337
1102	96-97	0.23281793150135854
1102	98-99	0.24264734167391566
1102	100	0.32965330965043904
1103	1	0.24858211296056254
1103	2	0.21552064016642447
1103	3	0.19285155222556227
1103	4	0.13474016965642477
1103	5	0.12121173280881692
1103	6	0.15558915765672765
1103	7	0.1540370485521052
1103	8	0.17407821528659895
1103	9	0.20198808211260655
1103	10-11	0.23051333686725428
1103	12-13	0.2163633198561925
1103	14-15	0.3428089478828227
1103	16-17	0.38732432646194326
1103	18-19	0.34009263074680973
1103	20-21	0.30776704657561993
1103	22-23	0.2993958335168472
1103	24-25	0.27856067891208625
1103	26-27	0.3154636139127689
1103	28-29	0.26809837510521817
1103	30-31	0.37774568310696566
1103	32-33	0.33528634396789414
1103	34-35	0.35938413064084074
1103	36-37	0.29354196579310354
1103	38-39	0.2933235016773281
1103	40-41	0.3415971137813614
1103	42-43	0.30279848250784624
1103	44-45	0.2721235348748934
1103	46-47	0.30358487401318257
1103	48-49	0.3759073828350452
1103	50-51	0.2683846698888033
1103	52-53	0.21760759090255277
1103	54-55	0.30108070229497486
1103	56-57	0.23574247617948174
1103	58-59	0.22705547061674025
1103	60-61	0.2376188428973478
1103	62-63	0.22091247306578055
1103	64-65	0.23339577653628396
1103	66-67	0.17495942061775338
1103	68-69	0.170033611440374
1103	70-71	0.17273807784174977
1103	72-73	0.20554632778033266
1103	74-75	0.21096218451153703
1103	76-77	0.2437993296179073
1103	78-79	0.1756919412277469
1103	80-81	0.15343520018546997
1103	82-83	0.1715428105358967
1103	84-85	0.14865787350890258
1103	86-87	0.18038628918681354
1103	88-89	0.30225011391770096
1103	90-91	0.216440447320835
1103	92-93	0.1926605164646844
1103	94-95	0.22385963771858286
1103	96-97	0.13893483657172112
1103	98-99	0.11805572663059394
1103	100	0.15542154533570596
1104	1	0.18808161126700185
1104	2	0.1578958681459639
1104	3	0.13142180872678466
1104	4	0.13027204798603975
1104	5	0.10133385777209014
1104	6	0.1632205819675221
1104	7	0.1311316884691749
1104	8	0.15776139441614134
1104	9	0.1776947493705876
1104	10-11	0.1794313695632681
1104	12-13	0.2184449323713622
1104	14-15	0.33961654147223186
1104	16-17	0.3940379425915239
1104	18-19	0.3442953537509794
1104	20-21	0.28432118790352234
1104	22-23	0.2900382784552846
1104	24-25	0.29853002294082387
1104	26-27	0.2878035986124985
1104	28-29	0.31563626544220824
1104	30-31	0.34966886024083266
1104	32-33	0.32870404127759656
1104	34-35	0.33721606606928134
1104	36-37	0.35384844235209556
1104	38-39	0.3355551510572852
1104	40-41	0.2980374316499734
1104	42-43	0.281393352483299
1104	44-45	0.2855559642358827
1104	46-47	0.3568250244658131
1104	48-49	0.4294243655778942
1104	50-51	0.3629230702347215
1104	52-53	0.3319524927535511
1104	54-55	0.4334200494835514
1104	56-57	0.4086476283783398
1104	58-59	0.3314174328206434
1104	60-61	0.23655170465721653
1104	62-63	0.30650007818582736
1104	64-65	0.29749405858230915
1104	66-67	0.30762687816783085
1104	68-69	0.28174670093030585
1104	70-71	0.33041780055211945
1104	72-73	0.35256194403187635
1104	74-75	0.33250403296128184
1104	76-77	0.2799517557636264
1104	78-79	0.2799624344396392
1104	80-81	0.3085222620351402
1104	82-83	0.2633558506880078
1104	84-85	0.22439177358040752
1104	86-87	0.2845095311805039
1104	88-89	0.28528330884643793
1104	90-91	0.23149298655548733
1104	92-93	0.28094091327380255
1104	94-95	0.2859587679316249
1104	96-97	0.26494723200602976
1104	98-99	0.21442489271056786
1104	100	0.2591695565780796
1105	1	0.12036249446323666
1105	2	0.09547094477612461
1105	3	0.08158172283192755
1105	4	0.10107205152625909
1105	5	0.03500205950692248
1105	6	0.08734946242406494
1105	7	0.13283462903844878
1105	8	0.1282243270043466
1105	9	0.12728035707517904
1105	10-11	0.07683075704088083
1105	12-13	0.13194068304887452
1105	14-15	0.21383483423669247
1105	16-17	0.2840459135757456
1105	18-19	0.2311928996051904
1105	20-21	0.18132505323555392
1105	22-23	0.21063692303913228
1105	24-25	0.20729573870819706
1105	26-27	0.18862235929209703
1105	28-29	0.19359542018057851
1105	30-31	0.23476717601329256
1105	32-33	0.23830054238194265
1105	34-35	0.2508190901826808
1105	36-37	0.23133517306627738
1105	38-39	0.2054614034149722
1105	40-41	0.1800955213830875
1105	42-43	0.17720895045601281
1105	44-45	0.21454634073335654
1105	46-47	0.1988150876764223
1105	48-49	0.27008145328314015
1105	50-51	0.24913647045409704
1105	52-53	0.20314526236830233
1105	54-55	0.18168031565370768
1105	56-57	0.14902769346255695
1105	58-59	0.1995579546215822
1105	60-61	0.15032186603065156
1105	62-63	0.1770400683302782
1105	64-65	0.1709119375670909
1105	66-67	0.1587649207444528
1105	68-69	0.10043561024372138
1105	70-71	0.10910977613874451
1105	72-73	0.22191993716701575
1105	74-75	0.21260659513006885
1105	76-77	0.1463759890778462
1105	78-79	0.15696696803241394
1105	80-81	0.16381508175791026
1105	82-83	0.15273773803684065
1105	84-85	0.12426564296783482
1105	86-87	0.12654142555356174
1105	88-89	0.20977428399656617
1105	90-91	0.11139287948097376
1105	92-93	0.1379466628820296
1105	94-95	0.15715313756670213
1105	96-97	0.11369380634086212
1105	98-99	0.1524093871584853
1105	100	0.13781928586184478
1106	1	0.14705879371543062
1106	2	0.13371575876370656
1106	3	0.13322588968876659
1106	4	0.10699234214138187
1106	5	0.10573449786211597
1106	6	0.14992219492969383
1106	7	0.14641072057050764
1106	8	0.15480637290741583
1106	9	0.15314059842222605
1106	10-11	0.18890630839732125
1106	12-13	0.18514661081127315
1106	14-15	0.298353163799284
1106	16-17	0.3408979337785567
1106	18-19	0.2933410736082678
1106	20-21	0.25463687046192973
1106	22-23	0.2166502253028284
1106	24-25	0.2334902682181621
1106	26-27	0.24905069942346358
1106	28-29	0.29191873368529997
1106	30-31	0.3217845737374674
1106	32-33	0.3100641950441556
1106	34-35	0.27420780043649273
1106	36-37	0.3031180424420299
1106	38-39	0.2608661089172486
1106	40-41	0.29288502530507543
1106	42-43	0.2858302734480773
1106	44-45	0.2800428376573407
1106	46-47	0.3023348760286382
1106	48-49	0.32086143282332813
1106	50-51	0.28847906158726033
1106	52-53	0.25764928946136223
1106	54-55	0.26133236723142517
1106	56-57	0.2682380041784924
1106	58-59	0.2558796073063334
1106	60-61	0.29152113883164077
1106	62-63	0.3171704372699651
1106	64-65	0.28784941387507956
1106	66-67	0.32577221226811304
1106	68-69	0.2947917427543487
1106	70-71	0.26534591375951067
1106	72-73	0.2921512394493959
1106	74-75	0.3481333145031513
1106	76-77	0.2644178685093479
1106	78-79	0.23351003086907696
1106	80-81	0.2550387632289315
1106	82-83	0.26171986687896265
1106	84-85	0.21505028796791237
1106	86-87	0.195222538284753
1106	88-89	0.2789101096288178
1106	90-91	0.24122131293806603
1106	92-93	0.23390509460976006
1106	94-95	0.2539768310939863
1106	96-97	0.24426013404674052
1106	98-99	0.2818183039174329
1106	100	0.18535181989560812
1107	1	0.03984603770663142
1107	2	0.012937583020253385
1107	3	0.018326802580659773
1107	4	0.05589082044568272
1107	5	0.0372065425746797
1107	6	0.08707293035841701
1107	7	0.10264837058782206
1107	8	0.07677718170640446
1107	9	0.11202819252791585
1107	10-11	0.13475776026886876
1107	12-13	0.14191930470534686
1107	14-15	0.21447382044961927
1107	16-17	0.2738347426633325
1107	18-19	0.1663927575993398
1107	20-21	0.1702192535417879
1107	22-23	0.12147264183551698
1107	24-25	0.14821746239623934
1107	26-27	0.1623117303312398
1107	28-29	0.17617070785193079
1107	30-31	0.15317794206481494
1107	32-33	0.20674273617450467
1107	34-35	0.12854478223409416
1107	36-37	0.13121002307327956
1107	38-39	0.1558645947531474
1107	40-41	0.14107384706049686
1107	42-43	0.2290626529190476
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2216	48-49	-0.45287396259382007
2216	50-51	-0.5376203057339808
2216	52-53	-0.48670050054147396
2216	54-55	-0.42431223904198845
2216	56-57	-0.5084432460638197
2216	58-59	-0.5229376908327339
2216	60-61	-0.4810819994699784
2216	62-63	-0.48108179036726284
2216	64-65	-0.4454889819865002
2216	66-67	-0.5098283391938807
2216	68-69	-0.41670247805973304
2216	70-71	-0.46508793451240393
2216	72-73	-0.41392732408380084
2216	74-75	-0.4018991218683112
2216	76-77	-0.38457589472206877
2216	78-79	-0.35894860324166444
2216	80-81	-0.3516753285534904
2216	82-83	-0.34895232538961807
2216	84-85	-0.3004196149064242
2216	86-87	-0.31690537836797716
2216	88-89	-0.19267665852801485
2216	90-91	-0.25502681076500266
2216	92-93	-0.2546167457278301
2216	94-95	-0.26550392946831636
2216	96-97	-0.3273393861071128
2216	98-99	-0.2951924640843231
2216	100	-0.3847786518987455
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	45.0
8	141.0
9	260.0
10	293.0
11	207.0
12	208.0
13	288.0
14	535.0
15	889.0
16	1785.0
17	3303.0
18	5581.0
19	8639.0
20	12617.0
21	18383.0
22	26508.0
23	30897.0
24	29227.0
25	25266.0
26	23840.0
27	26831.0
28	32749.0
29	40887.0
30	49616.0
31	63362.0
32	81023.0
33	106175.0
34	155642.0
35	242665.0
36	434428.0
37	983663.0
38	1276641.0
39	485810.0
40	1210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62216287583988	20.51509835123918	13.722717019687035	24.140021753233906
2	15.04204381382721	21.11829829282449	40.12557981129451	23.714078082053792
3	17.40391422886756	26.96610808498065	31.173649897064664	24.45632778908713
4	12.149054819962279	18.1041929616601	39.6768055362259	30.069946682151716
5	12.507968531674823	38.6461434705048	35.57025426871064	13.275633729109737
6	28.830037333926867	39.51050007698953	18.217258222889733	13.442204366193872
7	25.193632892421537	33.79962716000055	23.686060374370115	17.320679573207805
8	25.397435633007053	35.141569731048925	20.950324802871062	18.510669833072956
9	26.30631365245952	14.753976086521178	21.293188939506404	37.6465213215129
10-11	24.328052067188498	26.404012363259177	28.899591988464156	20.368343581088173
12-13	25.220721711370615	24.425133051788688	28.71051465878041	21.64363057806029
14-15	22.536002707203085	25.274587570033415	26.837488910852002	25.351920811911498
16-17	21.390491425446776	28.11676405798631	27.731943433490702	22.760801083076217
18-19	21.210638259856644	27.182443060329014	30.239011533571304	21.367907146243038
20-21	22.41682489500809	26.34057598234078	29.74546522010348	21.49713390254765
22-23	22.441521607380245	26.195631397320184	29.389114156048386	21.973732839251184
24-25	22.038091268334718	26.395098158895646	29.889399913709102	21.677410659060534
26-27	21.947898440776125	26.61140805493276	29.489005737112123	21.95168776717899
28-29	21.62085870237003	26.831697011979756	29.66036209081074	21.887082194839476
30-31	22.103382128082288	26.470338881935795	29.53003861153871	21.896240378443206
32-33	22.276841025267135	26.707823233178384	29.03375043109589	21.98158531045859
34-35	22.346193330574483	26.716015012916678	29.488924562360097	21.448867094148742
36-37	22.34467971824703	26.498785258093683	29.244101966370955	21.91243305728833
38-39	22.238590765551375	26.40139313691561	29.55756007255402	21.802456024978998
40-41	22.175969439747085	26.17790104036383	29.669735626855243	21.976393893033837
42-43	22.303763151387766	26.195835252974074	29.172667417654907	22.327734177983256
44-45	22.180483496095178	26.830270321607237	28.891876393572602	22.09736978872498
46-47	22.463801831199365	26.644437606619505	28.359751027492454	22.532009534688676
48-49	22.811401389744784	26.601703826576774	28.259015311847175	22.32787947183127
50-51	22.55964996305416	26.861447945938437	27.839319534623925	22.739582556383475
52-53	22.246341280031253	27.033270749294864	27.958169063722877	22.762218906951002
54-55	22.2884383601331	27.04061510058112	27.76184830443344	22.909098234852333
56-57	22.693451729627153	26.87460765238976	27.669885391028544	22.76205522695454
58-59	22.242903754249678	26.861077064928455	28.184495254499765	22.7115239263221
60-61	22.287620331061287	27.14623894121017	27.869715263405364	22.696425464323173
62-63	22.419612308692955	26.875057259606784	27.70783378573419	22.99749664596607
64-65	21.930267618125296	26.893959561569247	27.8319036991096	23.343869121195855
66-67	22.278381891614462	26.745771736106448	27.715370446761767	23.26047592551732
68-69	22.03542391174002	26.634116800575608	28.008526202182516	23.321933085501858
70-71	22.14834235589219	26.405587874582693	27.94115971709982	23.5049100524253
72-73	22.41249094911767	26.478450685612582	27.976502122685236	23.132556242584513
74-75	22.29497824734221	26.855439531520698	27.722308037396896	23.127274183740195
76-77	22.15865245035028	26.745185708037866	27.572300272375067	23.523861569236786
78-79	21.983343784090124	26.72307077410788	27.76939358869155	23.524191853110445
80-81	21.916931842274156	26.836451005428863	27.795160357293952	23.451456795003025
82-83	22.175104488966394	26.391189614107326	28.248475445602544	23.185230451323743
84-85	22.440802540589484	26.56045861655007	27.483284601447604	23.515454241412844
86-87	21.675983669483312	26.835690184494165	27.861731665042615	23.626594480979907
88-89	22.07893434816675	26.34532140481968	27.684221356541862	23.891522890471713
90-91	21.91774974002402	26.352570596429025	27.855178990103646	23.87450067344331
92-93	22.017609550048117	26.34822780294332	27.72174872365238	23.912413923356187
94-95	21.402917274187296	26.358798655462266	27.983890105828806	24.254393964521633
96-97	21.60957819529496	26.257043399053938	27.784445757388216	24.348932648262885
98-99	21.369044699685137	26.519783418878788	27.688752116352216	24.422419765083855
100	21.663778537278468	26.218054568844686	27.46534244734164	24.652824446535206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	175.0
1	121.0
2	230.5
3	399.5
4	267.0
5	201.0
6	445.5
7	1205.0
8	2314.5
9	2789.5
10	2142.0
11	1500.5
12	1638.0
13	1933.5
14	2242.5
15	2709.0
16	3199.0
17	3758.5
18	4451.5
19	5194.0
20	6099.0
21	7144.0
22	8612.0
23	10692.0
24	13242.5
25	16812.5
26	22702.5
27	29053.5
28	34076.0
29	41878.5
30	50819.0
31	58184.5
32	68760.5
33	79273.5
34	87452.0
35	96499.5
36	106529.0
37	119734.0
38	126000.0
39	124270.0
40	123721.0
41	122815.0
42	124775.0
43	132262.5
44	139553.0
45	151212.0
46	161181.5
47	167300.5
48	174094.0
49	169575.5
50	161024.5
51	159391.0
52	156502.0
53	150709.0
54	145315.5
55	132352.0
56	118921.0
57	103822.0
58	87727.0
59	78597.5
60	65892.0
61	51824.5
62	41232.0
63	31487.5
64	22255.5
65	14383.5
66	9648.5
67	7117.5
68	5305.5
69	3920.5
70	2930.0
71	2179.0
72	1683.0
73	1365.5
74	998.0
75	722.5
76	516.0
77	297.0
78	160.5
79	87.0
80	47.5
81	24.5
82	8.5
83	5.0
84	3.5
85	3.0
86	2.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0029259282087539455
2	0.0037653338424128648
3	2.1584716294086482E-4
4	0.05173137005149394
5	0.002182454647513189
6	0.005252280964894378
7	0.0
8	2.6381319914994594E-4
9	4.796603620908108E-5
10-11	1.0792358147043241E-4
12-13	0.0
14-15	0.0
16-17	2.877962172544864E-4
18-19	1.558896176795135E-4
20-21	2.3983018104540538E-4
22-23	0.0
24-25	0.0
26-27	2.518216900976756E-4
28-29	0.0
30-31	2.0385565388859455E-4
32-33	2.6381319914994594E-4
34-35	1.79872635784054E-4
36-37	1.6788112673178375E-4
38-39	8.154226155543782E-4
40-41	4.3169432588172963E-4
42-43	0.0
44-45	6.71524506927135E-4
46-47	0.0
48-49	5.9957545261351345E-5
50-51	0.0
52-53	5.636009254567026E-4
54-55	5.396179073521621E-4
56-57	1.0792358147043241E-4
58-59	8.51397142711189E-4
60-61	6.355499797703242E-4
62-63	2.6381319914994594E-4
64-65	4.556773439862702E-4
66-67	0.0013670320319588106
68-69	0.002806013118231243
70-71	0.0038492744057787563
72-73	0.003213724426008432
74-75	0.003033851790224378
76-77	0.004376900804078648
78-79	0.0014989386315337836
80-81	0.0012710999595406485
82-83	9.113546879725404E-4
84-85	8.03431106502108E-4
86-87	0.0
88-89	0.0
90-91	2.398301810454054E-5
92-93	1.0792358147043241E-4
94-95	1.3190659957497297E-4
96-97	5.156348892476216E-4
98-99	3.59745271568108E-5
100	0.07523472779394366
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4169617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.27936092118452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.92405221694318	32.48695162585757
2	16.419791472941608	16.18313660491355
3	6.968017847631256	10.30138399256028
4	3.592366927697714	7.081181855653697
5	2.139439700244552	5.271511057873106
6	1.3148499008389813	3.8876977692376706
7	0.8703165878436608	3.002205167362909
8	0.5961578095856871	2.35026206916447
9	0.4392519367296729	1.948144925288777
>10	1.6855888175122624	13.022501730842947
>50	0.03376130574803977	1.1010656823277403
>100	0.014412020494361888	1.2717394343675292
>500	9.533918990591215E-4	0.32089077205753197
>1k	7.80047917410749E-4	0.5763049630463114
>5k	8.667199082341656E-5	0.2533213322335784
>10k+	1.733439816468331E-4	0.9417010172122635
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11602	0.2782509760488793	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10446	0.25052660712003044	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5931	0.1422432803780299	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	3.597452715681081E-5	0.0
20-21	0.0	0.0	0.0	8.394056336589187E-5	0.0
22-23	0.0	0.0	0.0	1.6788112673178375E-4	0.0
24-25	0.0	0.0	0.0	3.477537625158378E-4	0.0
26-27	0.0	0.0	0.0	9.83303742286162E-4	0.0
28-29	0.0	0.0	0.0	0.0034175800798970264	0.0
30-31	0.0	0.0	0.0	0.01206345810658389	0.0
32-33	0.0	0.0	0.0	0.0335162678010954	0.0
34-35	0.0	0.0	0.0	0.06847151668846324	0.0
36-37	0.0	0.0	0.0	0.12315279796681566	0.0
38-39	0.0	0.0	0.0	0.20573832080980098	0.0
40-41	0.0	0.0	0.0	0.29806094900322977	0.0
42-43	0.0	0.0	0.0	0.3947005204554759	0.0
44-45	0.0	0.0	0.0	0.5159466684829805	0.0
46-47	0.0	0.0	0.0	0.6673154872497882	0.0
48-49	0.0	0.0	0.0	0.8307477641231796	0.0
50-51	0.0	0.0	0.0	1.0089775631670728	0.0
52-53	0.0	0.0	0.0	1.1854685933983866	0.0
54-55	0.0	0.0	0.0	1.3791794306287604	0.0
56-57	0.0	0.0	0.0	1.6100879289392767	0.0
58-59	0.0	0.0	0.0	1.8724741385120025	0.0
60-61	0.0	0.0	0.0	2.1508570211604567	0.0
62-63	0.0	0.0	0.0	2.4334369319772056	0.0
64-65	0.0	0.0	0.0	2.7308023734554037	0.0
66-67	0.0	0.0	0.0	3.0683033957315504	0.0
68-69	0.0	0.0	0.0	3.447690759127277	0.0
70-71	0.0	0.0	0.0	3.856889014026948	0.0
72-73	0.0	0.0	0.0	4.277862930815948	0.0
74-75	0.0	0.0	0.0	4.69863299195106	0.0
76-77	0.0	0.0	0.0	5.1683643845465905	0.0
78-79	0.0	0.0	0.0	5.6776197909784045	0.0
80-81	0.0	0.0	0.0	6.231435645048454	0.0
82-83	0.0	0.0	0.0	6.803394652314589	0.0
84-85	0.0	0.0	0.0	7.373195187951316	0.0
86-87	0.0	0.0	0.0	7.9626737899428175	0.0
88	0.0	0.0	0.0	8.426529343102736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7760	0.0	55.024338	1
GTATCAA	14315	0.0	44.150803	1
ATCAACG	17785	0.0	34.84664	3
CAACGCA	17765	0.0	34.711235	5
TATCAAC	18885	0.0	33.4402	2
AACGCAG	19655	0.0	31.827408	6
TCAACGC	19570	0.0	31.692274	4
ACGCAGA	20985	0.0	29.495258	7
CGCAGAG	21430	0.0	28.838923	8
GCAGAGT	23265	0.0	26.22087	9
GTGGTAT	2440	0.0	25.054409	1
GTACATG	14710	0.0	24.199919	1
TACATGG	14780	0.0	23.86144	2
ACATGGG	14730	0.0	23.367258	3
GAGTACT	15230	0.0	23.11333	12-13
AGAGTAC	21795	0.0	22.717518	10-11
CAGAGTA	22735	0.0	22.326057	10-11
CATGGGG	12345	0.0	21.292181	4
AGTACTT	16680	0.0	20.63917	12-13
TGGTATC	2870	0.0	20.480377	2
>>END_MODULE