FastQCFastQC Report
Mon 16 Jan 2017
ERR1146622_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146622_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6497651
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60850.039.9582441
GTATCAA102800.036.5993461
CAACGCA106300.032.7728465
ATCAACG110550.031.5994363
TATCAAC125800.029.5258482
AACGCAG124700.028.7948976
TCAACGC131850.026.7438354
ACGCAGA133750.025.9323087
GTACATG232450.025.027451
CGCAGAG138700.025.0062458
TACATGG231750.024.6294612
ACATGGG231700.023.741553
GTACTAG27700.023.090621
CATGGGG193550.022.4450914
CTAACGC13450.020.6242893
GCAGAGT165400.020.3720849
GGGCTAT45700.019.5400686
GTATAAG38750.018.9334341
ATGGGGG134300.018.7636765
GCTATTG41200.018.7074768
Produced by FastQC (version 0.11.5)