##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146622_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6497651
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125302820973303	31.0	31.0	34.0	28.0	34.0
2	31.405863595936438	31.0	31.0	34.0	28.0	34.0
3	31.502440959048123	33.0	31.0	34.0	28.0	34.0
4	35.0251760213037	37.0	35.0	37.0	32.0	37.0
5	34.98852292928629	37.0	35.0	37.0	32.0	37.0
6	35.11837016177077	37.0	35.0	37.0	32.0	37.0
7	35.04057912621038	37.0	35.0	37.0	32.0	37.0
8	35.01727670507388	37.0	35.0	37.0	32.0	37.0
9	36.631805709478705	39.0	37.0	39.0	32.0	39.0
10-11	36.611211420865786	39.0	37.0	39.0	32.0	39.0
12-13	36.55467298874624	39.0	37.0	39.0	32.0	39.0
14-15	37.88922281298272	40.0	38.0	41.0	32.0	41.0
16-17	37.91295438920927	40.0	38.0	41.0	32.0	41.0
18-19	37.92297824244484	40.0	38.0	41.0	32.0	41.0
20-21	38.01740590561112	40.0	38.0	41.0	32.0	41.0
22-23	38.02473501577724	40.0	38.0	41.0	32.0	41.0
24-25	38.035802861680324	40.0	38.0	41.0	32.0	41.0
26-27	38.0861555198948	40.0	38.0	41.0	32.0	41.0
28-29	38.065519985607104	40.0	38.0	41.0	32.0	41.0
30-31	38.02644001655368	40.0	38.0	41.0	32.0	41.0
32-33	37.99471870680651	40.0	38.0	41.0	32.0	41.0
34-35	37.94414758502727	40.0	38.0	41.0	32.0	41.0
36-37	37.873378086942495	40.0	38.0	41.0	31.5	41.0
38-39	37.81629461169891	40.0	38.0	41.0	31.0	41.0
40-41	37.75818214921054	40.0	38.0	41.0	31.0	41.0
42-43	37.608429646344504	40.0	37.0	41.0	31.0	41.0
44-45	37.483665789375266	40.0	37.0	41.0	31.0	41.0
46-47	37.36639987281558	40.0	37.0	41.0	30.0	41.0
48-49	37.253575022727446	40.0	36.0	41.0	30.0	41.0
50-51	37.11811968663753	40.0	36.0	41.0	30.0	41.0
52-53	36.974910471491924	39.5	35.5	41.0	30.0	41.0
54-55	36.84695723115938	39.0	35.0	41.0	30.0	41.0
56-57	36.644113772808055	39.0	35.0	41.0	30.0	41.0
58-59	36.435005050286634	39.0	35.0	41.0	30.0	41.0
60-61	36.20706359882979	38.0	35.0	40.0	29.0	41.0
62-63	35.96028841807601	38.0	35.0	40.0	29.0	41.0
64-65	35.66199323417032	37.0	35.0	40.0	29.0	41.0
66-67	35.41883574541015	37.0	34.0	40.0	29.0	41.0
68-69	35.159891397675864	36.0	34.0	39.0	29.0	41.0
70-71	34.88284897111279	36.0	34.0	39.0	28.5	41.0
72-73	34.55131854573291	35.0	34.0	39.0	28.0	40.5
74-75	34.23015902208352	35.0	34.0	37.5	28.0	40.0
76-77	33.96603564888296	35.0	34.0	37.0	28.0	39.0
78-79	33.67053993820228	35.0	33.5	36.5	27.5	39.0
80-81	33.44202720336934	35.0	33.0	36.0	27.0	39.0
82-83	33.18835114412886	35.0	33.0	36.0	27.0	37.0
84-85	33.0011387961588	35.0	33.0	35.5	27.0	37.0
86-87	32.803916984768804	35.0	33.0	35.0	26.5	36.5
88-89	32.642069957281485	35.0	33.0	35.0	26.0	36.0
90-91	32.507315028154025	35.0	33.0	35.0	26.0	36.0
92-93	32.36651599170223	35.0	33.0	35.0	25.5	36.0
94-95	32.25525108997082	35.0	33.0	35.0	25.0	35.5
96-97	32.16176330492358	35.0	33.0	35.0	25.0	35.0
98-99	32.059885564798726	35.0	33.0	35.0	25.0	35.0
100	30.892978093160128	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2690268732727503
1101	2	0.2559156626858119
1101	3	0.3043647311333153
1101	4	0.32089798627171007
1101	5	0.2625820488475199
1101	6	0.31222164609133785
1101	7	0.3486653530881654
1101	8	0.3673117015659315
1101	9	0.5049123131561046
1101	10-11	0.4818058207169571
1101	12-13	0.44288429332074486
1101	14-15	0.5631824997684731
1101	16-17	0.5555187906372225
1101	18-19	0.48437132250162307
1101	20-21	0.5552188773303897
1101	22-23	0.568555651845486
1101	24-25	0.5949929475191169
1101	26-27	0.5965259097294862
1101	28-29	0.5774387911685963
1101	30-31	0.5725208937099637
1101	32-33	0.6259670826247543
1101	34-35	0.5585119025691654
1101	36-37	0.5711034785227085
1101	38-39	0.5462886220787553
1101	40-41	0.5393964990431783
1101	42-43	0.5500214688547445
1101	44-45	0.4817450624233999
1101	46-47	0.4964565532340899
1101	48-49	0.5495417996897061
1101	50-51	0.47576287805867423
1101	52-53	0.48025410530350143
1101	54-55	0.4687898714064289
1101	56-57	0.47638751620531394
1101	58-59	0.47505294501353745
1101	60-61	0.4675867358975907
1101	62-63	0.45448579869439243
1101	64-65	0.47666662959648676
1101	66-67	0.4236105490681794
1101	68-69	0.3741641418723205
1101	70-71	0.40940047423681847
1101	72-73	0.39403509466718134
1101	74-75	0.4049033980750778
1101	76-77	0.372634375333341
1101	78-79	0.3553659115233643
1101	80-81	0.3005884371155716
1101	82-83	0.38833136775357247
1101	84-85	0.25051613815593043
1101	86-87	0.27765597902735095
1101	88-89	0.2890935909526178
1101	90-91	0.31498488342275266
1101	92-93	0.3106338241637374
1101	94-95	0.30026839243033976
1101	96-97	0.29977351303553945
1101	98-99	0.24827572471564707
1101	100	0.2814836653446946
1102	1	0.3016734066721618
1102	2	0.41077201836691657
1102	3	0.3452667174281139
1102	4	0.350302717689857
1102	5	0.2879677930408846
1102	6	0.4099965252683546
1102	7	0.4079381389656547
1102	8	0.47062255239781337
1102	9	0.5254122000389572
1102	10-11	0.5118962666225428
1102	12-13	0.5087992959395677
1102	14-15	0.6198786914134544
1102	16-17	0.5744291099366592
1102	18-19	0.5209555597227933
1102	20-21	0.5528902680102661
1102	22-23	0.5348637959546494
1102	24-25	0.5775386205638995
1102	26-27	0.5486204100626182
1102	28-29	0.525080521780005
1102	30-31	0.5548082485585937
1102	32-33	0.5382631594042024
1102	34-35	0.5174494063963735
1102	36-37	0.4523725678225432
1102	38-39	0.4365148894445454
1102	40-41	0.5180387619820124
1102	42-43	0.5460187494290949
1102	44-45	0.45840108337559826
1102	46-47	0.4857227522164749
1102	48-49	0.43179537227489817
1102	50-51	0.4648332171869072
1102	52-53	0.3874879153966191
1102	54-55	0.3716810408169948
1102	56-57	0.42514895922261786
1102	58-59	0.38629368567552547
1102	60-61	0.3979882268479926
1102	62-63	0.28962303707224635
1102	64-65	0.3880743219347593
1102	66-67	0.3746602758872868
1102	68-69	0.3317052625739976
1102	70-71	0.3327234364833771
1102	72-73	0.3497756389342328
1102	74-75	0.35929313824031794
1102	76-77	0.3066934734888065
1102	78-79	0.2650535533801701
1102	80-81	0.26175713109329735
1102	82-83	0.24869242283424597
1102	84-85	0.24113201548897223
1102	86-87	0.21944432480106713
1102	88-89	0.21580227760156134
1102	90-91	0.225316593923246
1102	92-93	0.23740571270636934
1102	94-95	0.26303345714198656
1102	96-97	0.2541306689589433
1102	98-99	0.28538158082035636
1102	100	0.3074736599463712
1103	1	0.18988841563774272
1103	2	0.2290715887972219
1103	3	0.20612104427301148
1103	4	0.24382013913763956
1103	5	0.2268139350576277
1103	6	0.28092370651334875
1103	7	0.2404458905302036
1103	8	0.24256748517035476
1103	9	0.34748077760381335
1103	10-11	0.3080810672727239
1103	12-13	0.30254542107228133
1103	14-15	0.40121645739041156
1103	16-17	0.37542510841951326
1103	18-19	0.4320479890789599
1103	20-21	0.4367936397361163
1103	22-23	0.4138735365006383
1103	24-25	0.45464917861629317
1103	26-27	0.43888664539628763
1103	28-29	0.42284444681668276
1103	30-31	0.4778360501705734
1103	32-33	0.48187641018994043
1103	34-35	0.4257463106996937
1103	36-37	0.4172481192184989
1103	38-39	0.44724143316403087
1103	40-41	0.37629934999418424
1103	42-43	0.4117650474149883
1103	44-45	0.367988118906041
1103	46-47	0.40655406098862557
1103	48-49	0.3679371410578156
1103	50-51	0.3689589993511433
1103	52-53	0.366881395493337
1103	54-55	0.3166181007049076
1103	56-57	0.3791263358731314
1103	58-59	0.3760254041805666
1103	60-61	0.3570076186246425
1103	62-63	0.37473046504946694
1103	64-65	0.3118651989702741
1103	66-67	0.3467244295139196
1103	68-69	0.2594631684666098
1103	70-71	0.24774068788173764
1103	72-73	0.26623202638666754
1103	74-75	0.30687194737627266
1103	76-77	0.3065664609089964
1103	78-79	0.2925091779597935
1103	80-81	0.26517548442807737
1103	82-83	0.2254810797442488
1103	84-85	0.25690797261803766
1103	86-87	0.25791703257921483
1103	88-89	0.2692703974636359
1103	90-91	0.305194115210206
1103	92-93	0.2687977926714069
1103	94-95	0.28590392366898243
1103	96-97	0.31651966121247455
1103	98-99	0.2550633891459171
1103	100	0.304506177088264
1104	1	0.017445723643877642
1104	2	0.08368514555290929
1104	3	0.07242361849806045
1104	4	0.05578613981496261
1104	5	0.07716876365115155
1104	6	0.1101446197192999
1104	7	0.14391047913589716
1104	8	0.18882255995314523
1104	9	0.27952032746043187
1104	10-11	0.2586119863517524
1104	12-13	0.21367779242225993
1104	14-15	0.30538775173511823
1104	16-17	0.3288314625399096
1104	18-19	0.30712491260879204
1104	20-21	0.29757004267504783
1104	22-23	0.35118781842560054
1104	24-25	0.3686811778234045
1104	26-27	0.3263308721808684
1104	28-29	0.31721034764296974
1104	30-31	0.35436359908846526
1104	32-33	0.3296551756837758
1104	34-35	0.3529789443247111
1104	36-37	0.34619130946736476
1104	38-39	0.29361131625611137
1104	40-41	0.3003371732159579
1104	42-43	0.26684989229921996
1104	44-45	0.22235044402660975
1104	46-47	0.2834697328719997
1104	48-49	0.2657842220058839
1104	50-51	0.22655059359694008
1104	52-53	0.2592826207745276
1104	54-55	0.20539172460264155
1104	56-57	0.24065620726359072
1104	58-59	0.15583715237041673
1104	60-61	0.20306961888589825
1104	62-63	0.1988002333558967
1104	64-65	0.2661773117319015
1104	66-67	0.20722642704414085
1104	68-69	0.14005882804900693
1104	70-71	0.18858296925445472
1104	72-73	0.2371356552297783
1104	74-75	0.19096796017001338
1104	76-77	0.1479014076502665
1104	78-79	0.15269548000260613
1104	80-81	0.1376496044370441
1104	82-83	0.1554609000674887
1104	84-85	0.15295823691039345
1104	86-87	0.12744736623739783
1104	88-89	0.09746026645595407
1104	90-91	0.14382620358728104
1104	92-93	0.13272869723475367
1104	94-95	0.15448317018091018
1104	96-97	0.1448297583087026
1104	98-99	0.16590593735671177
1104	100	0.23796162941485122
1105	1	0.19883052334847306
1105	2	0.26947451176125625
1105	3	0.22599105006891307
1105	4	0.2879766726681723
1105	5	0.20985584759945652
1105	6	0.2727910915791085
1105	7	0.28176234272525846
1105	8	0.2950028031577574
1105	9	0.3637843366923761
1105	10-11	0.4161734050290349
1105	12-13	0.4005965752687928
1105	14-15	0.5200008546551871
1105	16-17	0.4222343958851269
1105	18-19	0.3994636697615732
1105	20-21	0.4588547225574189
1105	22-23	0.4504439432802627
1105	24-25	0.476155481107881
1105	26-27	0.43647489067247847
1105	28-29	0.4495672786317968
1105	30-31	0.44277480191822605
1105	32-33	0.47089244774618777
1105	34-35	0.43093438990521094
1105	36-37	0.45890049116970744
1105	38-39	0.4432588042960859
1105	40-41	0.434310279345965
1105	42-43	0.46694833667940827
1105	44-45	0.41656309447055406
1105	46-47	0.4163919446206279
1105	48-49	0.42847038506613444
1105	50-51	0.43651914388185986
1105	52-53	0.44680338583911805
1105	54-55	0.3908729753361371
1105	56-57	0.4778839336961127
1105	58-59	0.4647418123866629
1105	60-61	0.42821456918048995
1105	62-63	0.3858214682608434
1105	64-65	0.4640572487631829
1105	66-67	0.4192602010702231
1105	68-69	0.35844892448528043
1105	70-71	0.3600469442135079
1105	72-73	0.3168126050435376
1105	74-75	0.3601567591151138
1105	76-77	0.30307587310690565
1105	78-79	0.3685029078130313
1105	80-81	0.29822977885860524
1105	82-83	0.3410355921086179
1105	84-85	0.3213307057317749
1105	86-87	0.3136033080581555
1105	88-89	0.2408380812211135
1105	90-91	0.2423016616715472
1105	92-93	0.35452239567445076
1105	94-95	0.3074160349029995
1105	96-97	0.2980722037166075
1105	98-99	0.2997449203299354
1105	100	0.40414414767090534
1106	1	0.10972607202178963
1106	2	0.17666127511440521
1106	3	0.17300325566306185
1106	4	0.20789284070215075
1106	5	0.18586359185817258
1106	6	0.26533089685679556
1106	7	0.22929528403290078
1106	8	0.295780642520981
1106	9	0.32972486546984925
1106	10-11	0.3311803394079078
1106	12-13	0.35112774084782217
1106	14-15	0.37231743451845745
1106	16-17	0.3834570537508668
1106	18-19	0.3690961392141645
1106	20-21	0.394190224661088
1106	22-23	0.4211436078935833
1106	24-25	0.393395623269555
1106	26-27	0.3872232318537172
1106	28-29	0.35992373181976944
1106	30-31	0.36311907125857346
1106	32-33	0.41503388911127104
1106	34-35	0.3962710181045921
1106	36-37	0.36816459363577536
1106	38-39	0.3470531318930554
1106	40-41	0.3291349337210363
1106	42-43	0.38198678792392116
1106	44-45	0.3453609024029163
1106	46-47	0.37536564184108556
1106	48-49	0.39454570442590153
1106	50-51	0.33176583905608936
1106	52-53	0.28479910970565214
1106	54-55	0.29807681728772906
1106	56-57	0.3131027309296286
1106	58-59	0.2837336461205666
1106	60-61	0.33108726519623133
1106	62-63	0.30899288623147925
1106	64-65	0.3192509318786918
1106	66-67	0.3324034180747901
1106	68-69	0.24085708128628625
1106	70-71	0.22812134415868002
1106	72-73	0.30663999912436424
1106	74-75	0.3271021511822454
1106	76-77	0.28954093343612897
1106	78-79	0.37416980513547315
1106	80-81	0.30604557161350243
1106	82-83	0.3134844483349397
1106	84-85	0.3036354729534878
1106	86-87	0.278660651597022
1106	88-89	0.27013244522569124
1106	90-91	0.29408380506439613
1106	92-93	0.2867995586226897
1106	94-95	0.27014528584302155
1106	96-97	0.313788847999497
1106	98-99	0.262018496883897
1106	100	0.2853099450558467
1107	1	0.03072634872542679
1107	2	0.11160681626575553
1107	3	0.05727235963207633
1107	4	0.07935507985388313
1107	5	0.10440134521929423
1107	6	0.1361966134470265
1107	7	0.17066657464519608
1107	8	0.10817523282043595
1107	9	0.17763147462237328
1107	10-11	0.18676606817438568
1107	12-13	0.18931392870489105
1107	14-15	0.17767155367514675
1107	16-17	0.22866514462090493
1107	18-19	0.21840200562010637
1107	20-21	0.24485694994143614
1107	22-23	0.3035725524419348
1107	24-25	0.3376441343697394
1107	26-27	0.2480091653830243
1107	28-29	0.25074278635755576
1107	30-31	0.22565766617524474
1107	32-33	0.2879098678019858
1107	34-35	0.23323378440510822
1107	36-37	0.24555541950361714
1107	38-39	0.24879087246183929
1107	40-41	0.2284956916258878
1107	42-43	0.27012833098042677
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2216	58-59	-0.42992788852388486
2216	60-61	-0.5044446591042515
2216	62-63	-0.46526331346741046
2216	64-65	-0.4700485029971233
2216	66-67	-0.4168123632633822
2216	68-69	-0.5267293894966585
2216	70-71	-0.4932885375799998
2216	72-73	-0.43450947059779566
2216	74-75	-0.39312415113271015
2216	76-77	-0.4829411886542161
2216	78-79	-0.4055170641723933
2216	80-81	-0.4070584867472533
2216	82-83	-0.3751322055297308
2216	84-85	-0.3348513262326094
2216	86-87	-0.3520794739019877
2216	88-89	-0.36460699463979296
2216	90-91	-0.40185960885301597
2216	92-93	-0.39608429411734747
2216	94-95	-0.4155431543736583
2216	96-97	-0.36060938878201654
2216	98-99	-0.3836861450033666
2216	100	-0.4020873184720699
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1901.0
1	467.0
2	349.0
3	347.0
4	248.0
5	340.0
6	488.0
7	1002.0
8	2737.0
9	3837.0
10	4530.0
11	6227.0
12	8600.0
13	11662.0
14	14540.0
15	15931.0
16	17468.0
17	19380.0
18	22029.0
19	24109.0
20	27271.0
21	30022.0
22	33958.0
23	39276.0
24	47845.0
25	59190.0
26	65070.0
27	63548.0
28	68202.0
29	82789.0
30	96831.0
31	111597.0
32	139976.0
33	187761.0
34	279612.0
35	427421.0
36	786268.0
37	1575023.0
38	1713027.0
39	506077.0
40	695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.405098682766344	19.873346294780653	13.304461994414593	24.417093028038412
2	15.028751120323516	20.551645759094257	40.1403368824361	24.27926623814613
3	18.00356465056906	26.221559728689797	30.25210692349894	25.5227686972422
4	12.557729477416252	17.377071349436978	39.258555206284356	30.806643966862413
5	13.427910050770835	38.302415384380794	35.066269470329416	13.203405094518958
6	30.18257150963921	37.99156859863689	18.433878287508694	13.391981604215209
7	26.341753519929217	32.847723167086045	23.264403183154617	17.546120129830122
8	25.517050800275786	35.45735898208445	20.795263471478485	18.230326746161282
9	26.434454096652647	15.567303168236165	20.717290577841368	37.28095215726982
10-11	24.743427970962202	26.327076054984367	28.49141076206696	20.438085211986465
12-13	25.15642455691516	24.144400641530112	28.09598033396651	22.60319446758822
14-15	22.80548679609062	25.288736487298735	25.474539137884015	26.431237578726623
16-17	22.568306650588685	28.056619899536745	26.257196162416367	23.117877287458207
18-19	22.57584115618993	27.993182665129424	27.38206840083964	22.048907777841013
20-21	23.846068371020294	27.015409318468258	26.64631075077738	22.492211559734066
22-23	23.88343699352934	26.824880726473076	26.34100993261972	22.95067234737786
24-25	23.35801235080348	27.078254869511042	26.693106637536495	22.870626142148982
26-27	23.205718801711075	27.236546255156526	26.393182864570953	23.16455207856145
28-29	22.959298129799596	27.550159687130126	26.4289882256396	23.06155395743068
30-31	23.410575615009634	27.21169651966897	26.34143662850017	23.03629123682122
32-33	23.443481518323793	27.39573419090459	25.946349219894294	23.21443507087732
34-35	23.558797698983895	27.302180999188003	26.42739685134084	22.711624450487264
36-37	23.438463933140653	27.109914622046944	26.39700940717876	23.054612037633646
38-39	23.453979767284025	27.098535058200557	26.514804227200145	22.93268094731527
40-41	23.36396169454991	26.79169597779219	26.725115374289278	23.119226953368624
42-43	23.155332051398766	26.899799453182972	26.578838282614353	23.36603021280391
44-45	23.165546129042742	27.24178766609165	26.531048419332613	23.061617785532995
46-47	23.207555103867815	27.034843690746147	26.36505471031827	23.39254649506777
48-49	23.39376288634781	26.807600920480652	26.56843061315235	23.230205580019188
50-51	23.260013506202874	27.00581284296944	26.294095410971817	23.44007823985587
52-53	23.0464909335551	27.100620866560483	26.304979371843896	23.54790882804052
54-55	23.031628342503197	26.97315831099754	26.408764497340925	23.58644884915834
56-57	23.32626569691547	26.801970003521497	26.411355233147276	23.460409066415757
58-59	23.10998974937894	26.847122169717874	26.687223881020085	23.355664199883105
60-61	23.009301687169938	27.035187924705955	26.578907518143613	23.376602869980495
62-63	23.22208566425864	26.721369787634053	26.39836261900852	23.65818192909879
64-65	22.790508581526623	26.83739598050348	26.524257184060108	23.847838253909785
66-67	23.217378966361263	26.578548927390912	26.439219434399902	23.764852671847926
68-69	22.770541383170176	26.673819359009084	26.672032341322844	23.8836069164979
70-71	22.860308947326622	26.495428783983133	26.58341070201205	24.060851566678192
72-73	23.047842042814988	26.61040707238236	26.680909367924162	23.660841516878495
74-75	22.94369750074102	26.879713032294962	26.644334871247217	23.532254595716807
76-77	22.854535375181563	26.592736439528498	26.62851559042801	23.92421259486193
78-79	22.80135999073763	26.671488378244863	26.606180351681953	23.920971279335557
80-81	22.66118303507639	26.693388483926896	26.883880799106404	23.761547681890313
82-83	22.952420180266845	26.31640654458915	27.25526330364834	23.47590997149566
84-85	23.08755902357862	26.53546036465595	26.674174440190722	23.702806171574707
86-87	22.508656829083712	26.738173044149733	26.93911238376202	23.81405774300454
88-89	22.862468450059893	26.24777856378757	26.842228424031195	24.04752456212134
90-91	22.803270817942497	26.16524179393545	26.970745298905925	24.060742089216127
92-93	22.94962987598502	26.13730461419398	26.97071811429884	23.942347395522155
94-95	22.422889461910884	26.074419449378734	27.278117369346887	24.224573719363494
96-97	22.536609448638174	26.125355927869393	27.0471446054929	24.290890017999534
98-99	22.397135557689484	26.352967856368696	26.90018036027994	24.349716225661876
100	22.745168524071175	26.07535197089042	26.72047444233509	24.459005062703312
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2387.0
1	1542.0
2	730.0
3	749.0
4	703.0
5	630.5
6	605.5
7	588.5
8	589.0
9	621.0
10	592.5
11	586.0
12	587.0
13	618.5
14	697.5
15	868.5
16	1029.5
17	1170.0
18	1492.5
19	1999.0
20	2701.0
21	3938.0
22	5932.0
23	8735.5
24	12606.5
25	17839.0
26	24893.0
27	33225.5
28	40365.0
29	50635.0
30	63946.5
31	77379.5
32	94127.0
33	108576.5
34	121474.5
35	134174.0
36	146950.5
37	164500.5
38	174010.5
39	175133.5
40	178159.0
41	180198.5
42	183380.0
43	193649.5
44	209348.5
45	229834.5
46	248725.5
47	265067.5
48	274987.5
49	271069.0
50	264363.5
51	269587.5
52	271043.5
53	265082.5
54	258145.5
55	235726.0
56	213992.0
57	189765.0
58	163482.5
59	145965.5
60	121500.5
61	96292.5
62	76733.0
63	59178.5
64	43234.0
65	29856.0
66	21590.0
67	15688.0
68	11214.0
69	8278.5
70	6323.0
71	4662.0
72	3431.0
73	2645.5
74	2108.0
75	1539.5
76	1046.5
77	677.5
78	363.0
79	219.5
80	165.0
81	118.5
82	87.5
83	65.0
84	48.5
85	39.0
86	29.5
87	25.0
88	22.5
89	16.5
90	13.5
91	14.0
92	14.0
93	12.0
94	11.5
95	12.0
96	12.0
97	11.0
98	12.0
99	12.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09375695924573357
2	0.06142219703705232
3	0.07708939738376222
4	0.07824366067060234
5	0.07916707130007444
6	0.0881703249374274
7	0.08426121993932884
8	0.08678520899321925
9	0.0708871559891413
10-11	0.07815901469623407
12-13	0.06810153392356715
14-15	0.07709709247234116
16-17	0.07892082846554856
18-19	0.07825135575918128
20-21	0.06978675832235372
22-23	0.07390363071208349
24-25	0.07627371799439521
26-27	0.06990987973961667
28-29	0.07866689054244373
30-31	0.08303000576669939
32-33	0.08492299755711717
34-35	0.08694680585337686
36-37	0.08763166873690201
38-39	0.08909373556689949
40-41	0.08495377791143292
42-43	0.09080204523142286
44-45	0.08738542590237611
46-47	0.08398419675048721
48-49	0.0929951454764191
50-51	0.08955544088163553
52-53	0.09479579620388968
54-55	0.08682368443611392
56-57	0.09371078871425997
58-59	0.0975275526494113
60-61	0.09615782688236102
62-63	0.097096627688991
64-65	0.09911274089667173
66-67	0.10100573268708954
68-69	0.09832784186162045
70-71	0.10072101440966898
72-73	0.09808159902709457
74-75	0.10168290048203574
76-77	0.09912043598525067
78-79	0.1057536023402919
80-81	0.10119810990156289
82-83	0.10595367464334418
84-85	0.10159055941908852
86-87	0.09832014677304152
88-89	0.09780457583825294
90-91	0.09802773340704203
92-93	0.09196400360684191
94-95	0.0915330786464216
96-97	0.08950927035016193
98-99	0.10394525652424239
100	0.24525786318778894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6497651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.359350556035366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.51136735610666	32.16446529935209
2	15.2046385124337	14.705728878011545
3	6.741135221019688	9.779907638967842
4	3.684804902903297	7.127790881203937
5	2.1891552330787554	5.293306266901741
6	1.3805699500853152	4.005807970998241
7	0.9971407997348235	3.3754757041671253
8	0.6882768386832656	2.662769673718708
9	0.48262748355462826	2.1005596498675914
>10	2.0550063548915865	16.047656866664227
>50	0.051778453986674806	1.652866753182745
>100	0.013298457179669222	0.997461592037273
>500	1.33624228052062E-4	0.03609016325364928
>1k	6.68121138839355E-5	0.050112661673263434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.539017715786828E-5	0.0
2	0.0	0.0	0.0	1.0773124010507798E-4	0.0
3	0.0	0.0	0.0	1.0773124010507798E-4	0.0
4	0.0	0.0	0.0	1.692919487365511E-4	0.0
5	0.0	0.0	0.0	2.1546248021015596E-4	0.0
6	0.0	0.0	0.0	2.1546248021015596E-4	3.078035431573656E-5
7	0.0	0.0	0.0	2.1546248021015596E-4	3.078035431573656E-5
8	0.0	0.0	0.0	2.1546248021015596E-4	3.078035431573656E-5
9	0.0	0.0	0.0	2.3085265736802422E-4	4.6170531473604845E-5
10-11	0.0	0.0	0.0	5.540463776832581E-4	4.6170531473604845E-5
12-13	0.0	0.0	0.0	5.617414662621923E-4	1.1542632868401211E-4
14-15	0.0	0.0	0.0	6.00216909156863E-4	1.539017715786828E-4
16-17	0.0	0.0	0.0	6.156070863147313E-4	1.7698703731548523E-4
18-19	0.0	0.0	0.0	6.617776177883361E-4	2.0007230305228767E-4
20-21	0.0	0.0	0.0	6.771677949462044E-4	2.0776739163122181E-4
22-23	0.0	0.0	0.0	7.695088578934141E-4	2.462428345258925E-4
24-25	0.0	0.0	0.0	9.926664266825041E-4	2.6932810026269495E-4
26-27	0.0	0.0	0.0	0.001415896298523882	2.9241336599949736E-4
28-29	0.0	0.0	0.0	0.0035397407463097046	3.0780354315736565E-4
30-31	0.0	0.0	0.0	0.01092702578208648	3.616691632099046E-4
32-33	0.0	0.0	0.0	0.02988002895200127	4.1553478326244363E-4
34-35	0.0	0.0	0.0	0.059175231172003545	4.4631513757818016E-4
36-37	0.0	0.0	0.0	0.10747730218197316	4.4631513757818016E-4
38-39	0.0	0.0	0.0	0.1805883387704264	4.4631513757818016E-4
40-41	0.0	0.0	0.0	0.26356447891707324	4.4631513757818016E-4
42-43	0.0	0.0	0.0	0.3504805044161344	4.4631513757818016E-4
44-45	0.0	0.0	0.0	0.4619284723048376	4.6170531473604845E-4
46-47	0.0	0.0	0.0	0.5980238089118668	5.001807576307191E-4
48-49	0.0	0.0	0.0	0.7473623929632416	5.848267319989947E-4
50-51	0.0	1.539017715786828E-5	0.0	0.9083051705916492	6.00216909156863E-4
52-53	0.0	1.539017715786828E-5	0.0	1.070509942747002	6.617776177883361E-4
54-55	0.0	1.539017715786828E-5	0.0	1.251152147137481	7.233383264198092E-4
56-57	0.0	1.539017715786828E-5	0.0	1.466245263095848	7.387285035776775E-4
58-59	0.0	1.539017715786828E-5	0.0	1.70974864608764	7.387285035776775E-4
60-61	0.0	1.539017715786828E-5	0.0	1.9689807901347733	7.387285035776775E-4
62-63	0.0	1.539017715786828E-5	0.0	2.235184684434421	8.156793893670189E-4
64-65	0.0	1.539017715786828E-5	0.0	2.5155475417193074	8.541548322616897E-4
66-67	0.0	1.539017715786828E-5	0.0	2.828906938830664	9.234106294720969E-4
68-69	0.0	1.539017715786828E-5	0.0	3.1843661655573685	9.234106294720969E-4
70-71	0.0	1.539017715786828E-5	0.0	3.5709828059401776	9.464958952088993E-4
72-73	0.0	1.539017715786828E-5	0.0	3.969472968000282	0.0010465320467350431
74-75	0.0	1.539017715786828E-5	0.0	4.373403557685693	0.0010850074896297138
76-77	0.0	1.539017715786828E-5	0.0	4.815624908139879	0.0011080927553665163
78-79	0.0	1.539017715786828E-5	0.0	5.299907612766521	0.001138873109682253
80-81	0.0	1.539017715786828E-5	0.0	5.825451382353407	0.001154263286840121
82-83	0.0	1.539017715786828E-5	0.0	6.375457838532725	0.0011773485525769236
84-85	7.69508857893414E-6	1.539017715786828E-5	0.0	6.922347783837575	0.0012004338183137261
86-87	1.539017715786828E-5	1.539017715786828E-5	0.0	7.489752835293862	0.0012389092612083968
88	1.539017715786828E-5	1.539017715786828E-5	0.0	7.939853956452878	0.001261994526945199
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6085	0.0	39.958244	1
GTATCAA	10280	0.0	36.599346	1
CAACGCA	10630	0.0	32.772846	5
ATCAACG	11055	0.0	31.599436	3
TATCAAC	12580	0.0	29.525848	2
AACGCAG	12470	0.0	28.794897	6
TCAACGC	13185	0.0	26.743835	4
ACGCAGA	13375	0.0	25.932308	7
GTACATG	23245	0.0	25.02745	1
CGCAGAG	13870	0.0	25.006245	8
TACATGG	23175	0.0	24.629461	2
ACATGGG	23170	0.0	23.74155	3
GTACTAG	2770	0.0	23.09062	1
CATGGGG	19355	0.0	22.445091	4
CTAACGC	1345	0.0	20.624289	3
GCAGAGT	16540	0.0	20.372084	9
GGGCTAT	4570	0.0	19.540068	6
GTATAAG	3875	0.0	18.933434	1
ATGGGGG	13430	0.0	18.763676	5
GCTATTG	4120	0.0	18.707476	8
>>END_MODULE