FastQCFastQC Report
Mon 16 Jan 2017
ERR1146622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6497651
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159700.24578112921115647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149180.22959066284107904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77980.12001260147705686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113850.058.734931
GTATCAA217200.043.0630571
CAACGCA265450.034.6873365
ATCAACG266550.034.523273
TATCAAC278750.033.4861682
AACGCAG286550.032.427156
TCAACGC286900.032.156464
ACGCAGA308250.029.8378077
CGCAGAG316850.028.9834428
GCAGAGT354200.025.6087239
GTGGTAT32200.025.2650451
GTACTAG25000.025.017361
GTACATG226300.023.0865691
GAGTACT222750.023.0300712-13
TACATGG228050.022.6614382
AGAGTAC318300.022.56182310-11
CAGAGTA343600.022.27522710-11
ACATGGG226600.022.0998573
CTAACGC10800.021.333993
CATGGGG191800.020.863194
Produced by FastQC (version 0.11.5)