##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146622_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6497651 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31270377556443 33.0 31.0 34.0 30.0 34.0 2 32.37286259295859 34.0 31.0 34.0 30.0 34.0 3 32.48604364869704 34.0 31.0 34.0 30.0 34.0 4 36.01710464289325 37.0 35.0 37.0 35.0 37.0 5 36.05717758617691 37.0 35.0 37.0 35.0 37.0 6 36.132626698479186 37.0 36.0 37.0 35.0 37.0 7 36.106005693442135 37.0 36.0 37.0 35.0 37.0 8 36.116166288401764 37.0 36.0 37.0 35.0 37.0 9 37.895007134116625 39.0 38.0 39.0 35.0 39.0 10-11 37.8502835870994 39.0 38.0 39.0 35.0 39.0 12-13 37.82025858267857 39.0 38.0 39.0 35.0 39.0 14-15 39.181232417684484 40.0 39.0 41.0 36.0 41.0 16-17 39.13174768851081 40.0 39.0 41.0 36.0 41.0 18-19 39.22832589808225 40.5 39.0 41.0 36.0 41.0 20-21 39.2822775492251 41.0 39.0 41.0 36.0 41.0 22-23 39.25273294918425 41.0 39.0 41.0 36.0 41.0 24-25 39.23859576329969 41.0 39.0 41.0 36.0 41.0 26-27 39.16209157740236 41.0 39.0 41.0 35.5 41.0 28-29 39.10536700109009 40.0 39.0 41.0 35.0 41.0 30-31 38.97118735678478 40.0 38.5 41.0 35.0 41.0 32-33 38.872995410187464 40.0 38.0 41.0 35.0 41.0 34-35 38.80297841481483 40.0 38.0 41.0 35.0 41.0 36-37 38.73903284433098 40.0 38.0 41.0 35.0 41.0 38-39 38.654458665139146 40.0 38.0 41.0 35.0 41.0 40-41 38.55355319945623 40.0 38.0 41.0 35.0 41.0 42-43 38.448834201775384 40.0 38.0 41.0 34.0 41.0 44-45 38.29718793761007 40.0 38.0 41.0 34.0 41.0 46-47 38.05795009611935 40.0 38.0 41.0 33.5 41.0 48-49 37.766601037821204 40.0 37.0 41.0 33.0 41.0 50-51 37.6429043357361 40.0 37.0 41.0 33.0 41.0 52-53 37.525093991659446 40.0 37.0 41.0 33.0 41.0 54-55 37.35433951438758 40.0 36.0 41.0 32.5 41.0 56-57 37.18158993149986 39.5 36.0 41.0 32.0 41.0 58-59 36.982120307785074 39.0 35.0 41.0 32.0 41.0 60-61 36.764218946200714 39.0 35.0 41.0 32.0 41.0 62-63 36.51228543976893 38.5 35.0 41.0 31.5 41.0 64-65 36.22957581132012 37.5 35.0 40.0 31.0 41.0 66-67 35.95797804468107 37.0 35.0 40.0 31.0 41.0 68-69 35.66637681833019 36.5 35.0 39.5 31.0 41.0 70-71 35.371670008130636 36.0 35.0 39.0 31.0 41.0 72-73 35.0634243821344 35.5 35.0 39.0 30.5 41.0 74-75 34.75987714637182 35.0 35.0 38.0 30.0 40.0 76-77 34.47615161232882 35.0 34.0 37.0 30.0 39.0 78-79 34.20897190384648 35.0 34.0 37.0 30.0 39.0 80-81 33.97152386300834 35.0 34.0 36.0 30.0 39.0 82-83 33.73669484556804 35.0 34.0 36.0 30.0 37.0 84-85 33.547923241799225 35.0 34.0 35.5 29.0 37.0 86-87 33.37589538126932 35.0 34.0 35.0 29.0 37.0 88-89 33.196938709081174 35.0 34.0 35.0 29.0 36.0 90-91 33.12178408781881 35.0 34.0 35.0 29.0 36.0 92-93 33.03038136397292 35.0 34.0 35.0 29.0 36.0 94-95 32.966692193840515 35.0 34.0 35.0 29.0 36.0 96-97 32.91525352777488 35.0 34.0 35.0 29.0 35.0 98-99 32.85200890290968 35.0 34.0 35.0 29.0 35.0 100 32.12084120861524 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3022070070881071 1101 2 0.27989946110730557 1101 3 0.29920759507179895 1101 4 0.22826465813671604 1101 5 0.21018068801655687 1101 6 0.2514516837779439 1101 7 0.2536190018644362 1101 8 0.23705839472862777 1101 9 0.2955290165887732 1101 10-11 0.29684744641124894 1101 12-13 0.30998799961589185 1101 14-15 0.46012615538738544 1101 16-17 0.49381869726778405 1101 18-19 0.44713410709086787 1101 20-21 0.41147561047132086 1101 22-23 0.4297964136216237 1101 24-25 0.4344013318499407 1101 26-27 0.4235394313877521 1101 28-29 0.43331803317187223 1101 30-31 0.515507956585715 1101 32-33 0.4683373081253066 1101 34-35 0.44650163460854486 1101 36-37 0.4341416413918253 1101 38-39 0.4167850918054157 1101 40-41 0.4570580340253656 1101 42-43 0.4386776470553997 1101 44-45 0.3942229365322447 1101 46-47 0.45957128634518796 1101 48-49 0.5099290181245806 1101 50-51 0.5314296412986224 1101 52-53 0.4868854020469726 1101 54-55 0.483768429718225 1101 56-57 0.44088093513429527 1101 58-59 0.4386520838954553 1101 60-61 0.39237520103773704 1101 62-63 0.4238003622054336 1101 64-65 0.36091636958278883 1101 66-67 0.28179391196851356 1101 68-69 0.2757604232331943 1101 70-71 0.26029082667275105 1101 72-73 0.24187942987081357 1101 74-75 0.2638967412863451 1101 76-77 0.23974335606349229 1101 78-79 0.24678582088507284 1101 80-81 0.24705615356107558 1101 82-83 0.25363863505572937 1101 84-85 0.2542775000864026 1101 86-87 0.22905654979907553 1101 88-89 0.30854454036026624 1101 90-91 0.28392141191002196 1101 92-93 0.29045537220817863 1101 94-95 0.29932734877748146 1101 96-97 0.2926326950477218 1101 98-99 0.32574151452160294 1101 100 0.31098165434840297 1102 1 0.23708202466580985 1102 2 0.20706156835669987 1102 3 0.22456248256492728 1102 4 0.1483232338880569 1102 5 0.17436396986692415 1102 6 0.18400095505698744 1102 7 0.1999078714460012 1102 8 0.21995000693464561 1102 9 0.28363858269676 1102 10-11 0.2808048808982946 1102 12-13 0.24966322012900122 1102 14-15 0.4137195447431026 1102 16-17 0.4573440123120278 1102 18-19 0.43191548718896655 1102 20-21 0.4031848840396677 1102 22-23 0.39749637530994875 1102 24-25 0.3860359419879984 1102 26-27 0.3726808293188526 1102 28-29 0.3751532782947322 1102 30-31 0.43338987857594446 1102 32-33 0.42143373393765415 1102 34-35 0.3702343424800958 1102 36-37 0.40247980669929717 1102 38-39 0.3656369284712042 1102 40-41 0.40089548317442336 1102 42-43 0.45221417235747197 1102 44-45 0.4384396345684607 1102 46-47 0.4520869729926602 1102 48-49 0.5416698569214873 1102 50-51 0.5137346537626328 1102 52-53 0.4789819270644031 1102 54-55 0.5055504707295384 1102 56-57 0.4431455990090427 1102 58-59 0.4310530690083283 1102 60-61 0.42120632749575293 1102 62-63 0.44077756524416145 1102 64-65 0.4591397633089258 1102 66-67 0.3933405011523661 1102 68-69 0.3261960069319727 1102 70-71 0.33673270056866045 1102 72-73 0.3910364270994364 1102 74-75 0.3942281007192534 1102 76-77 0.32158501352054003 1102 78-79 0.35087947181811074 1102 80-81 0.37608205164713127 1102 82-83 0.36332802669118536 1102 84-85 0.373252601500603 1102 86-87 0.33979352237736293 1102 88-89 0.44115864517726067 1102 90-91 0.36029092502320026 1102 92-93 0.33997563682697063 1102 94-95 0.3693262708359981 1102 96-97 0.36083601621377426 1102 98-99 0.36336266584593346 1102 100 0.4333470248641049 1103 1 0.21407796422381864 1103 2 0.21059147761022246 1103 3 0.23875018117239932 1103 4 0.15026648583857138 1103 5 0.15357851868803607 1103 6 0.14083892505532702 1103 7 0.16028512272828976 1103 8 0.16405243326705943 1103 9 0.2134997709005546 1103 10-11 0.25394541940941195 1103 12-13 0.24490326782674998 1103 14-15 0.37703290153474 1103 16-17 0.4030018623815792 1103 18-19 0.35931773302286985 1103 20-21 0.31024398101799733 1103 22-23 0.3301599051828674 1103 24-25 0.3432213045286616 1103 26-27 0.32729091605745 1103 28-29 0.33296903079042295 1103 30-31 0.3542805304696728 1103 32-33 0.3121167828089497 1103 34-35 0.30466399684967627 1103 36-37 0.3647051975479272 1103 38-39 0.35167194303046756 1103 40-41 0.3911738773124327 1103 42-43 0.36612248511710277 1103 44-45 0.3246240630823678 1103 46-47 0.30535892201017134 1103 48-49 0.41901656530558995 1103 50-51 0.3442371829494846 1103 52-53 0.2844610500300604 1103 54-55 0.31648578851777387 1103 56-57 0.2841694741193308 1103 58-59 0.24807769691355475 1103 60-61 0.22519264417447005 1103 62-63 0.20405265095085667 1103 64-65 0.24862057543600713 1103 66-67 0.18095940323324555 1103 68-69 0.10227212287708909 1103 70-71 0.14824056183955747 1103 72-73 0.1583838103481554 1103 74-75 0.18433977336356122 1103 76-77 0.15814020125767314 1103 78-79 0.12415876212875077 1103 80-81 0.13427022275133993 1103 82-83 0.17058303831910848 1103 84-85 0.11337987654955128 1103 86-87 0.1571652225811917 1103 88-89 0.17820988128337234 1103 90-91 0.1782261234585576 1103 92-93 0.19508583706909377 1103 94-95 0.18806177792878742 1103 96-97 0.17089119316064938 1103 98-99 0.15533624200086393 1103 100 0.18138216953381203 1104 1 0.1586735099754364 1104 2 0.16218889979587914 1104 3 0.1653265327742517 1104 4 0.15177089400737742 1104 5 0.10117412456313701 1104 6 0.13371740552305766 1104 7 0.1262841771326464 1104 8 0.1333156684262491 1104 9 0.1574795509905016 1104 10-11 0.1788274813197006 1104 12-13 0.1840358606485637 1104 14-15 0.2751176835025575 1104 16-17 0.3584993287596987 1104 18-19 0.26859771217703354 1104 20-21 0.25607545507116924 1104 22-23 0.27064729568726165 1104 24-25 0.2465535686225735 1104 26-27 0.253859870168057 1104 28-29 0.24952761293893388 1104 30-31 0.2903970226895325 1104 32-33 0.2998118109664034 1104 34-35 0.27798143463498803 1104 36-37 0.29380379172642535 1104 38-39 0.28914104387936845 1104 40-41 0.27623686676168546 1104 42-43 0.27550682173707486 1104 44-45 0.26227089243269575 1104 46-47 0.27317043725453516 1104 48-49 0.3244357333905157 1104 50-51 0.28185034616618054 1104 52-53 0.23167068425091486 1104 54-55 0.2667010309805491 1104 56-57 0.24024654903353593 1104 58-59 0.21580238599152324 1104 60-61 0.2063715204570542 1104 62-63 0.14618992732818725 1104 64-65 0.1561073800666435 1104 66-67 0.16364374446909835 1104 68-69 0.17119458656323872 1104 70-71 0.16251411555547435 1104 72-73 0.09115897654731953 1104 74-75 0.1085411905945648 1104 76-77 0.08605966929955144 1104 78-79 0.13438207616028564 1104 80-81 0.11828579160199126 1104 82-83 0.10662121526173962 1104 84-85 0.0991928281904535 1104 86-87 0.13743932152241456 1104 88-89 0.15653247674300275 1104 90-91 0.09602820124023737 1104 92-93 0.1223098277068928 1104 94-95 0.14297640880397466 1104 96-97 0.15179468273465346 1104 98-99 0.14217231197191893 1104 100 0.19557380358263998 1105 1 0.18364959085147348 1105 2 0.1856897683159886 1105 3 0.16984525522799032 1105 4 0.1599169330426875 1105 5 0.1152566882395476 1105 6 0.11548684742503923 1105 7 0.15752992535146149 1105 8 0.13634742124423127 1105 9 0.2075420484664221 1105 10-11 0.20237188499449843 1105 12-13 0.20372134949823106 1105 14-15 0.3090311774951431 1105 16-17 0.3543161633430998 1105 18-19 0.2857184649219917 1105 20-21 0.2515129008613286 1105 22-23 0.2466656896727315 1105 24-25 0.24713677003946088 1105 26-27 0.24613663644012007 1105 28-29 0.27819695082160933 1105 30-31 0.30236678182688337 1105 32-33 0.2547124279020778 1105 34-35 0.2653733299469323 1105 36-37 0.23507809506785549 1105 38-39 0.2775908037057917 1105 40-41 0.2917711638265459 1105 42-43 0.3071387034651494 1105 44-45 0.3171113360610889 1105 46-47 0.3987878012216157 1105 48-49 0.5053728251335059 1105 50-51 0.46576150768529345 1105 52-53 0.3892583165606496 1105 54-55 0.43953097406487274 1105 56-57 0.36187967221646034 1105 58-59 0.3729656469384537 1105 60-61 0.3595985366596963 1105 62-63 0.3971372722259403 1105 64-65 0.3717546668447511 1105 66-67 0.38434520229112223 1105 68-69 0.34008092325632333 1105 70-71 0.3206687014569596 1105 72-73 0.35511139745784703 1105 74-75 0.393934784934288 1105 76-77 0.32231603798430086 1105 78-79 0.33856650153966683 1105 80-81 0.33547628364284776 1105 82-83 0.31992990671280097 1105 84-85 0.3088493893861326 1105 86-87 0.2876635213404555 1105 88-89 0.35918942859832725 1105 90-91 0.3347324213467644 1105 92-93 0.3319021153774102 1105 94-95 0.32051212305410104 1105 96-97 0.29993576781460973 1105 98-99 0.31231272094302653 1105 100 0.24657140225018281 1106 1 0.08572883946978749 1106 2 0.08648817775369366 1106 3 0.09343636332310723 1106 4 0.08462259515176385 1106 5 0.09784547489302753 1106 6 0.11118573664992937 1106 7 0.15538651111302926 1106 8 0.13062053158476772 1106 9 0.12872510760190892 1106 10-11 0.160825158439323 1106 12-13 0.13464013442357015 1106 14-15 0.2650843563960592 1106 16-17 0.3217989018153702 1106 18-19 0.21717623392628838 1106 20-21 0.22067927943108856 1106 22-23 0.21445281266350946 1106 24-25 0.2072608829884217 1106 26-27 0.21200741368145515 1106 28-29 0.19819141526127737 1106 30-31 0.23642660286545691 1106 32-33 0.2682505537953972 1106 34-35 0.2192603454260933 1106 36-37 0.24163441638631866 1106 38-39 0.2379699927083152 1106 40-41 0.2518459460292988 1106 42-43 0.1888407777539527 1106 44-45 0.18706899406006983 1106 46-47 0.26514323410538054 1106 48-49 0.3423629281469829 1106 50-51 0.2767125300527553 1106 52-53 0.24591592410590835 1106 54-55 0.2397496625657638 1106 56-57 0.2541526970860204 1106 58-59 0.22609832311673017 1106 60-61 0.24744900665648828 1106 62-63 0.20019554230940884 1106 64-65 0.2848688624866611 1106 66-67 0.2624473798580951 1106 68-69 0.21655234533562862 1106 70-71 0.2496871051674887 1106 72-73 0.22165246679691109 1106 74-75 0.23072050179826675 1106 76-77 0.2283949762690014 1106 78-79 0.22713755915967937 1106 80-81 0.23237427764169638 1106 82-83 0.19087774464021834 1106 84-85 0.20490892780899372 1106 86-87 0.1952054036787345 1106 88-89 0.2540284766780232 1106 90-91 0.21078945624539358 1106 92-93 0.2126442899925749 1106 94-95 0.22279158008255706 1106 96-97 0.22724294720730853 1106 98-99 0.2311747729256055 1106 100 0.24875639335058963 1107 1 0.14703839746808 1107 2 0.09489816077604729 1107 3 0.1014110969758022 1107 4 0.07774187303120783 1107 5 0.061254076536371826 1107 6 0.1030288192675144 1107 7 0.08924566692841296 1107 8 0.10626557354649435 1107 9 0.12439058306315331 1107 10-11 0.14053684544055045 1107 12-13 0.13520111976662008 1107 14-15 0.23172200501195306 1107 16-17 0.294822147381133 1107 18-19 0.20907772700287097 1107 20-21 0.20377365505258638 1107 22-23 0.20513606101235382 1107 24-25 0.19031795656346162 1107 26-27 0.2135821215783551 1107 28-29 0.20486568459306653 1107 30-31 0.216692766737971 1107 32-33 0.19477247996504587 1107 34-35 0.16225442747647634 1107 36-37 0.172027284213641 1107 38-39 0.14398491160163474 1107 40-41 0.1499186094531737 1107 42-43 0.19716476017284634 1107 44-45 0.20041257516135147 1107 46-47 0.21154618840606787 1107 48-49 0.30508795842447256 1107 50-51 0.20672875149610093 1107 52-53 0.20507238659053684 1107 54-55 0.22717059960814368 1107 56-57 0.18935608495682033 1107 58-59 0.20326709618584005 1107 60-61 0.2065595087414991 1107 62-63 0.19693180844461722 1107 64-65 0.16984890335152159 1107 66-67 0.17833383745576015 1107 68-69 0.20694809250658608 1107 70-71 0.1712699569434406 1107 72-73 0.17730914699713196 1107 74-75 0.1646905369440148 1107 76-77 0.0969765615401954 1107 78-79 0.12482074643477148 1107 80-81 0.144593438689526 1107 82-83 0.1236723187674329 1107 84-85 0.16345305915155706 1107 86-87 0.16182920106877674 1107 88-89 0.22626320778722686 1107 90-91 0.15437411392170475 1107 92-93 0.1591058633228286 1107 94-95 0.17904611983520624 1107 96-97 0.17071116901902172 1107 98-99 0.17435480367211653 1107 100 0.03945658404828123 1108 1 0.03379994738195791 1108 2 0.03252616281434939 1108 3 0.021615290540843546 1108 4 0.03182250241929552 1108 5 0.025949950816794853 1108 6 0.057670534261553996 1108 7 0.04344786417669866 1108 8 0.04342815348595508 1108 9 0.07323851249189772 1108 10-11 0.05082427801740863 1108 12-13 0.0642104206260754 1108 14-15 0.13473456986117327 1108 16-17 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-0.2706856998141518 2214 82-83 -0.2597333180301362 2214 84-85 -0.27293463120942363 2214 86-87 -0.22308729795796722 2214 88-89 -0.1748278459841046 2214 90-91 -0.20360075344470374 2214 92-93 -0.2574181268565283 2214 94-95 -0.2486742376120361 2214 96-97 -0.2642569124775562 2214 98-99 -0.21210436271675093 2214 100 -0.33891430283469504 2215 1 -0.40273572208838715 2215 2 -0.37981874219467215 2215 3 -0.3942050501721326 2215 4 -0.2685665687159968 2215 5 -0.23168009275526913 2215 6 -0.261338544053487 2215 7 -0.27436096287328127 2215 8 -0.24966865388469017 2215 9 -0.38358659151129615 2215 10-11 -0.3501753148169229 2215 12-13 -0.35233496862091584 2215 14-15 -0.41798350024124886 2215 16-17 -0.41073586548831287 2215 18-19 -0.4455789607673708 2215 20-21 -0.47247110329080755 2215 22-23 -0.47206338716186025 2215 24-25 -0.46225697175522384 2215 26-27 -0.5287012137090414 2215 28-29 -0.47091851739439505 2215 30-31 -0.41656985061643326 2215 32-33 -0.5093565980567263 2215 34-35 -0.48873647022170275 2215 36-37 -0.5077596014879688 2215 38-39 -0.47693738670085395 2215 40-41 -0.5201954747056945 2215 42-43 -0.4741510717851156 2215 44-45 -0.46097555765531695 2215 46-47 -0.4953735436890412 2215 48-49 -0.4430275663322263 2215 50-51 -0.42822213988477387 2215 52-53 -0.44639440472683845 2215 54-55 -0.41294761657956514 2215 56-57 -0.505234000155788 2215 58-59 -0.5459384747922371 2215 60-61 -0.4640325736353077 2215 62-63 -0.45155364776969265 2215 64-65 -0.38748290040622635 2215 66-67 -0.4158190172702021 2215 68-69 -0.47604561412936164 2215 70-71 -0.4742909269494433 2215 72-73 -0.4314689633169877 2215 74-75 -0.4562382066920918 2215 76-77 -0.445592109104922 2215 78-79 -0.42858441100718636 2215 80-81 -0.409781969460127 2215 82-83 -0.3579667234331865 2215 84-85 -0.36440707956430174 2215 86-87 -0.3286716530913836 2215 88-89 -0.27849860907426915 2215 90-91 -0.3288233529481843 2215 92-93 -0.30633445950858373 2215 94-95 -0.3071904840650177 2215 96-97 -0.33514650801784995 2215 98-99 -0.3149083359735343 2215 100 -0.46952800055522914 2216 1 -0.39548640150376 2216 2 -0.4227744655066914 2216 3 -0.38390577250942926 2216 4 -0.29411126964195233 2216 5 -0.28312548639545554 2216 6 -0.2772872547817826 2216 7 -0.2731493750260796 2216 8 -0.285051865205574 2216 9 -0.37319814352714076 2216 10-11 -0.4159528641193049 2216 12-13 -0.4016153471004529 2216 14-15 -0.5087350153838983 2216 16-17 -0.477728689338889 2216 18-19 -0.4777516535531916 2216 20-21 -0.5029086404023104 2216 22-23 -0.4975536107484615 2216 24-25 -0.49966206418123704 2216 26-27 -0.5191510258462984 2216 28-29 -0.5726627477002921 2216 30-31 -0.4752602941780566 2216 32-33 -0.5888306496338984 2216 34-35 -0.5348594285105079 2216 36-37 -0.5476982806747088 2216 38-39 -0.5651909086330562 2216 40-41 -0.5369526423771092 2216 42-43 -0.5642002492807165 2216 44-45 -0.5880949217483646 2216 46-47 -0.5293331818553781 2216 48-49 -0.4650795613116685 2216 50-51 -0.5713040327991621 2216 52-53 -0.571445656202819 2216 54-55 -0.5534263186626944 2216 56-57 -0.49357364032757545 2216 58-59 -0.5530973797384746 2216 60-61 -0.5895522120301564 2216 62-63 -0.6060724470319983 2216 64-65 -0.5229690916987835 2216 66-67 -0.5550914786296985 2216 68-69 -0.4492858989757025 2216 70-71 -0.5020068977635006 2216 72-73 -0.4744866499413831 2216 74-75 -0.47036717165811126 2216 76-77 -0.49627883033682707 2216 78-79 -0.4432275089414688 2216 80-81 -0.41760464944240994 2216 82-83 -0.40731846490550794 2216 84-85 -0.4084259869370044 2216 86-87 -0.3625711150103328 2216 88-89 -0.27219613039824964 2216 90-91 -0.3570977112138465 2216 92-93 -0.3575267864537821 2216 94-95 -0.30995451778082383 2216 96-97 -0.31875691287869046 2216 98-99 -0.3952759552227576 2216 100 -0.4577832406026623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 58.0 8 202.0 9 365.0 10 383.0 11 261.0 12 285.0 13 449.0 14 729.0 15 1391.0 16 2705.0 17 4634.0 18 7922.0 19 12241.0 20 17826.0 21 26691.0 22 38664.0 23 45986.0 24 44280.0 25 37875.0 26 35956.0 27 40691.0 28 50496.0 29 62429.0 30 78946.0 31 100465.0 32 128460.0 33 168703.0 34 248596.0 35 387232.0 36 707075.0 37 1607484.0 38 1982837.0 39 653936.0 40 1398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.06718042418855 20.009517524310933 13.57166030933801 24.35164174216251 2 15.29578980012882 20.89389120476003 39.593378002242424 24.216940992868725 3 17.92661367410111 26.409553867318923 30.744788762567833 24.91904369601214 4 12.701482268944414 17.76730237645911 38.64482271408088 30.8863926405156 5 13.01675765281741 38.23785392217233 35.1104554068336 13.634933018176659 6 29.782719610180436 38.37187844610854 17.929237467804256 13.916164475906772 7 25.67135810639231 33.416604464691076 23.142643878940845 17.76939354997576 8 25.787336954037553 34.82906308468425 20.62236221170454 18.761237749573652 9 26.515375022565856 14.812249784498979 20.809249622440362 37.8631255704948 10-11 24.826035088243103 26.065383827821346 28.3151368730201 20.793444210915442 12-13 25.68953764983684 23.92549245873624 28.31309345485007 22.071876436576847 14-15 22.97323824561482 24.79071085628734 26.416837350183325 25.819213547914522 16-17 22.002282216571103 27.68298382002485 27.09579284325263 23.218941120151413 18-19 21.942328485345726 26.82445923288047 29.43761389223433 21.79559838953947 20-21 23.129052674914536 25.8388683579224 29.068026648147118 21.964052319015952 22-23 23.090375275618836 25.694601018121784 28.841784515665736 22.373239190593647 24-25 22.61991295007996 25.950170300005343 29.129526962897824 22.30038978701688 26-27 22.50863313714922 26.142007295574178 28.808676322072728 22.540683245203873 28-29 22.242738183383505 26.40919002882734 28.903791539434792 22.444280248354367 30-31 22.739029799527874 26.03632874815327 28.78963758591297 22.435003866405882 32-33 22.890224998753393 26.204484215490066 28.338983593417666 22.566307192338876 34-35 22.930912064296578 26.173035607777255 28.708425575164643 22.18762675276152 36-37 22.81576942797075 26.047469326787994 28.638029588704285 22.49873165653698 38-39 22.87580225701589 25.987532946749926 28.760069477896938 22.376595318337248 40-41 22.811660943034237 25.76765007259579 28.796365808906703 22.62432317546327 42-43 22.80748842928006 25.846532847024257 28.470196383277585 22.875782340418098 44-45 22.79562187419225 26.27101721314749 28.243470051844366 22.689890860815893 46-47 23.026482955147944 26.128842561719612 27.750490138667033 23.094184344465408 48-49 23.36974626120396 26.183711286848244 27.57396851306084 22.87257393888695 50-51 23.114584024288163 26.5049092356607 27.162523810527833 23.217982929523302 52-53 22.89245046154528 26.606689917199027 27.1788139950742 23.322045626181488 54-55 22.91015949336659 26.50140351022995 27.181727757337015 23.406709239066448 56-57 23.166610607890288 26.33718265134388 27.17894923306438 23.317257507701445 58-59 22.876334328495112 26.352900727168098 27.538130019783445 23.232634924553334 60-61 22.828862808035165 26.561294148798765 27.406542816598307 23.203300226567766 62-63 22.99970273824783 26.234261498462402 27.25639407104779 23.509641692241974 64-65 22.59211409936955 26.28721267716736 27.38979854899502 23.73087467446807 66-67 22.979689392528616 26.117799060882575 27.298975466288272 23.60353608030054 68-69 22.59886879569065 26.124848018468644 27.521169680646402 23.755113505194306 70-71 22.715264154359836 26.008668181160232 27.317714211047534 23.9583534534324 72-73 22.89290617285471 26.09737159637275 27.3666944723185 23.643027758454046 74-75 22.84359806633023 26.4078282536795 27.215847965906836 23.532725714083437 76-77 22.78320160020525 26.224477067154307 27.058547039427133 23.93377429321331 78-79 22.659684271658392 26.33396020779738 27.01373642292488 23.992619097619354 80-81 22.54731716641357 26.450449543669198 27.18519900227607 23.817034287641153 82-83 22.813195255854154 26.109500603914547 27.511639668013217 23.565664472218085 84-85 23.02156141998983 26.280352633516497 26.864881685982446 23.833204260511227 86-87 22.38486991450755 26.523127592283362 27.121513162451038 23.970489330758042 88-89 22.644183259457918 26.083626221229792 27.01803313228119 24.254157387031096 90-91 22.556418286335695 26.00768170089815 27.114858996318592 24.321041016447566 92-93 22.660188323499778 26.10175618904622 27.032600560602766 24.205454926851235 94-95 22.16122319403593 25.991811340247995 27.302909538987553 24.544055926728525 96-97 22.27125292385477 26.044806032723166 27.080845406774884 24.60309563664718 98-99 22.164434587534572 26.23941317784307 26.93431147286288 24.66184076175948 100 22.388019473253692 25.981197951023617 26.824685732802923 24.806096842919768 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 207.0 1 132.5 2 229.5 3 408.0 4 284.0 5 242.0 6 511.5 7 1307.5 8 2546.5 9 3193.5 10 2649.0 11 2076.0 12 2213.0 13 2636.5 14 3165.0 15 3836.5 16 4648.0 17 5440.5 18 6409.0 19 7479.0 20 8654.5 21 10313.5 22 12506.0 23 15575.5 24 19159.5 25 23910.5 26 30918.5 27 38732.5 28 45123.0 29 54627.0 30 66968.0 31 78959.0 32 94143.5 33 107641.0 34 119324.0 35 130727.5 36 143030.5 37 160077.0 38 169586.0 39 171004.5 40 174018.5 41 176363.0 42 179324.5 43 189237.5 44 204994.5 45 226175.5 46 244931.5 47 261408.0 48 271814.0 49 267591.0 50 262007.0 51 267047.5 52 267529.5 53 262676.5 54 257158.5 55 234756.5 56 212257.0 57 188143.0 58 162248.5 59 144985.0 60 120778.0 61 95120.5 62 75441.0 63 58147.5 64 41780.0 65 28200.0 66 20280.0 67 14721.0 68 10458.0 69 7577.0 70 5716.0 71 4260.0 72 3004.5 73 2212.5 74 1801.0 75 1291.0 76 826.5 77 471.5 78 222.0 79 91.0 80 46.0 81 21.5 82 13.5 83 6.5 84 1.0 85 0.5 86 2.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0025085988767325302 2 0.0033242782660995486 3 1.5390177157868282E-4 4 0.05052595160928157 5 0.002185405156417296 6 0.004401590667150328 7 1.539017715786828E-5 8 3.0780354315736565E-4 9 3.078035431573656E-5 10-11 6.925579721040727E-5 12-13 0.0 14-15 2.3085265736802422E-5 16-17 3.1549863173629977E-4 18-19 1.0003615152614382E-4 20-21 2.077673916312218E-4 22-23 0.0 24-25 0.0 26-27 2.0007230305228764E-4 28-29 0.0 30-31 2.2315756878909008E-4 32-33 2.3085265736802422E-4 34-35 1.7698703731548523E-4 36-37 3.8475442894670706E-5 38-39 6.925579721040727E-4 40-41 4.4631513757818016E-4 42-43 0.0 44-45 4.6940040331498257E-4 46-47 0.0 48-49 6.925579721040727E-5 50-51 0.0 52-53 3.385838974731022E-4 54-55 2.1546248021015593E-4 56-57 1.0003615152614382E-4 58-59 7.310334149987433E-4 60-61 5.617414662621923E-4 62-63 1.6159686015761697E-4 64-65 2.5393792310482666E-4 66-67 9.388008066299651E-4 68-69 0.0023239167508381105 70-71 0.003224242114573405 72-73 0.0026317202939954766 74-75 0.0024778185224167935 76-77 0.0036089965435201123 78-79 0.001100397666787582 80-81 9.541909837878335E-4 82-83 6.617776177883361E-4 84-85 4.155347832624436E-4 86-87 1.539017715786828E-5 88-89 0.0 90-91 2.3085265736802422E-5 92-93 3.078035431573656E-5 94-95 1.0003615152614382E-4 96-97 3.3088880889416806E-4 98-99 1.4620668299974868E-4 100 0.07461157886134542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6497651.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.34059987651584 #Duplication Level Percentage of deduplicated Percentage of total 1 64.51802145333552 29.252857955401506 2 15.99294561755728 14.502594961850843 3 7.229315986283996 9.833445705450064 4 3.8518997997827245 6.985897903455199 5 2.321988658426278 5.264017933975684 6 1.5046042710616496 4.0931796136021825 7 1.0051906276592175 3.1903162233824287 8 0.7154517297377978 2.5951208487202115 9 0.5393045149527937 2.200715120166577 >10 2.2398722565891185 16.357747870323532 >50 0.05839759617059927 1.7757050557119027 >100 0.020422060881227964 1.622596686268429 >500 0.0013975284125111857 0.4233400721488264 >1k 9.78269887664084E-4 0.6767991777632567 >5k 6.987642054743457E-5 0.24705926356136482 >10k+ 1.3975284109486914E-4 0.9786056082179324 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15970 0.24578112921115647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14918 0.22959066284107904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7798 0.12001260147705686 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.539017715786828E-5 0.0 2 0.0 0.0 0.0 1.5390177157868282E-4 0.0 3 0.0 0.0 0.0 1.5390177157868282E-4 0.0 4 0.0 0.0 0.0 2.7702318884162907E-4 0.0 5 0.0 0.0 0.0 2.9241336599949736E-4 0.0 6 0.0 0.0 0.0 2.9241336599949736E-4 3.078035431573656E-5 7 0.0 0.0 0.0 2.9241336599949736E-4 3.078035431573656E-5 8 0.0 0.0 0.0 2.9241336599949736E-4 3.078035431573656E-5 9 0.0 0.0 0.0 3.6936425178883876E-4 3.078035431573656E-5 10-11 0.0 0.0 0.0 5.848267319989947E-4 3.078035431573656E-5 12-13 0.0 0.0 0.0 5.925218205779289E-4 6.156070863147314E-5 14-15 0.0 0.0 0.0 6.617776177883361E-4 1.539017715786828E-4 16-17 0.0 0.0 0.0 6.771677949462044E-4 2.0776739163122181E-4 18-19 0.0 0.0 0.0 6.925579721040727E-4 2.2315756878909008E-4 20-21 0.0 0.0 0.0 7.07948149261941E-4 2.847182774205632E-4 22-23 0.0 0.0 0.0 8.002892122091507E-4 3.385838974731022E-4 24-25 0.0 0.0 0.0 0.0010234467809982408 3.9244951752564117E-4 26-27 0.0 0.0 0.0 0.0014466766528396183 4.0014460610457534E-4 28-29 0.0 0.0 0.0 0.0036243867206779802 4.309249604203119E-4 30-31 0.0 0.0 0.0 0.011111707907980899 4.3862004899924604E-4 32-33 0.0 0.0 0.0 0.030195527583737565 4.7709549189391674E-4 34-35 0.0 0.0 0.0 0.059660021752476394 4.92485669051785E-4 36-37 0.0 0.0 0.0 0.10819294541981403 4.92485669051785E-4 38-39 0.0 0.0 0.0 0.18157331010852998 5.232660233675216E-4 40-41 0.0 0.0 0.0 0.2649649850384393 5.386562005253899E-4 42-43 0.0 0.0 0.0 0.3523196305864996 5.463512891043241E-4 44-45 0.0 0.0 0.0 0.46374451320946597 5.617414662621923E-4 46-47 0.0 0.0 0.0 0.5999552761451792 6.002169091568631E-4 48-49 0.0 0.0 0.0 0.749770955688448 6.617776177883361E-4 50-51 0.0 0.0 0.0 0.9118679966036958 6.771677949462044E-4 52-53 0.0 0.0 0.0 1.0752424222230466 7.233383264198093E-4 54-55 0.0 0.0 0.0 1.257577546100891 7.541186807355459E-4 56-57 0.0 0.0 0.0 1.4743943619009392 7.6181376931448E-4 58-59 0.0 0.0 0.0 1.7200600647834117 7.695088578934141E-4 60-61 0.0 0.0 0.0 1.981054384115121 7.772039464723482E-4 62-63 0.0 0.0 0.0 2.24919744073666 8.69545009419558E-4 64-65 0.0 0.0 0.0 2.5320381165439634 8.926302751563603E-4 66-67 0.0 0.0 0.0 2.8483293424038933 9.695811609457018E-4 68-69 0.0 0.0 0.0 3.2072513589911185 0.0010003615152614383 70-71 0.0 0.0 0.0 3.5980079570293944 0.0010157516924193066 72-73 0.0 0.0 0.0 4.000968965553859 0.0011080927553665163 74-75 0.0 0.0 0.0 4.409016427629 0.0011388731096822527 76-77 0.0 0.0 0.0 4.855385430827233 0.0011619583754190553 78-79 0.0 0.0 0.0 5.34330791235171 0.0011696534639979894 80-81 0.0 0.0 0.0 5.871083257626487 0.00120812890689266 82-83 0.0 0.0 0.0 6.423221253342169 0.0012927748812609358 84-85 0.0 0.0 0.0 6.972442810486436 0.0012927748812609358 86-87 0.0 0.0 0.0 7.543133664765929 0.0013235552355766722 88 0.0 0.0 0.0 7.994550646071942 0.0013389454127345406 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11385 0.0 58.73493 1 GTATCAA 21720 0.0 43.063057 1 CAACGCA 26545 0.0 34.687336 5 ATCAACG 26655 0.0 34.52327 3 TATCAAC 27875 0.0 33.486168 2 AACGCAG 28655 0.0 32.42715 6 TCAACGC 28690 0.0 32.15646 4 ACGCAGA 30825 0.0 29.837807 7 CGCAGAG 31685 0.0 28.983442 8 GCAGAGT 35420 0.0 25.608723 9 GTGGTAT 3220 0.0 25.265045 1 GTACTAG 2500 0.0 25.01736 1 GTACATG 22630 0.0 23.086569 1 GAGTACT 22275 0.0 23.03007 12-13 TACATGG 22805 0.0 22.661438 2 AGAGTAC 31830 0.0 22.561823 10-11 CAGAGTA 34360 0.0 22.275227 10-11 ACATGGG 22660 0.0 22.099857 3 CTAACGC 1080 0.0 21.33399 3 CATGGGG 19180 0.0 20.86319 4 >>END_MODULE