FastQCFastQC Report
Mon 16 Jan 2017
ERR1146621_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146621_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480078
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG67350.018.286894
GTGATCG7450.017.661778
ACATGGG159350.017.4345843
GGCGTCG7550.017.4278378
TACATGG173650.017.3526442
GTACTAG10650.017.2183361
GTATATA23600.016.536451
GTATAAG20150.016.3342481
GTACATG198500.016.2731781
CTAGTAC15450.015.8216233
GTACCCG4852.910383E-1115.511521
GAACCGT4551.8735591E-1015.4923956
ATGGGGG43100.015.2695755
TTAGGGC29900.015.2502413
CGGGCGT8350.015.1955366
TGATCGC8850.014.8667499
TCTAACG5551.6370905E-1114.39922
CTAACGC4905.711627E-1014.390353
CTGTGCG14400.014.3579169
GTATTAT16250.014.1781271
Produced by FastQC (version 0.11.5)