##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146621_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3480078
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179939932380826	31.0	31.0	34.0	28.0	34.0
2	31.24686515647063	31.0	31.0	34.0	28.0	34.0
3	31.39165501462898	33.0	31.0	34.0	28.0	34.0
4	34.94186365937775	37.0	35.0	37.0	32.0	37.0
5	34.92744961463507	37.0	35.0	37.0	32.0	37.0
6	35.07984907234838	37.0	35.0	37.0	32.0	37.0
7	34.96793261530345	37.0	35.0	37.0	32.0	37.0
8	34.96451114026755	37.0	35.0	37.0	32.0	37.0
9	36.601724156757406	39.0	37.0	39.0	32.0	39.0
10-11	36.6098163604379	39.0	37.0	39.0	32.0	39.0
12-13	36.58282156319485	39.0	37.0	39.0	32.0	39.0
14-15	37.8928103048265	40.0	38.0	41.0	32.0	41.0
16-17	37.890628744528144	40.0	38.0	41.0	32.0	41.0
18-19	37.88101976449953	40.0	38.0	41.0	32.0	41.0
20-21	37.9882206663184	40.0	38.0	41.0	32.0	41.0
22-23	37.971325355351226	40.0	38.0	41.0	32.0	41.0
24-25	37.98277394931952	40.0	38.0	41.0	32.0	41.0
26-27	38.036038129030445	40.0	38.0	41.0	32.0	41.0
28-29	38.003276363345876	40.0	38.0	41.0	32.0	41.0
30-31	37.962946807514086	40.0	38.0	41.0	32.0	41.0
32-33	37.929916657040444	40.0	38.0	41.0	32.0	41.0
34-35	37.88012984191734	40.0	38.0	41.0	32.0	41.0
36-37	37.813983192330745	40.0	38.0	41.0	31.0	41.0
38-39	37.762617389610234	40.0	38.0	41.0	31.0	41.0
40-41	37.721449203150044	40.0	38.0	41.0	31.0	41.0
42-43	37.55718894806381	40.0	37.5	41.0	31.0	41.0
44-45	37.42498602042828	40.0	37.0	41.0	30.5	41.0
46-47	37.311272189876206	40.0	37.0	41.0	30.0	41.0
48-49	37.213779978494735	40.0	37.0	41.0	30.0	41.0
50-51	37.094384235065995	40.0	36.0	41.0	30.0	41.0
52-53	36.99290777390622	40.0	36.0	41.0	30.0	41.0
54-55	36.88873999375876	39.5	36.0	41.0	30.0	41.0
56-57	36.705512491386685	39.0	35.0	41.0	30.0	41.0
58-59	36.52886659436943	39.0	35.0	41.0	29.5	41.0
60-61	36.3151456662753	39.0	35.0	40.5	29.0	41.0
62-63	36.09554943883442	38.0	35.0	40.0	29.0	41.0
64-65	35.82725186044681	38.0	35.0	40.0	29.0	41.0
66-67	35.5834272967445	37.0	34.5	40.0	28.5	41.0
68-69	35.31943192077878	37.0	34.0	39.5	28.0	41.0
70-71	35.00702326212229	36.0	34.0	39.0	28.0	41.0
72-73	34.65314628005464	36.0	34.0	39.0	28.0	40.5
74-75	34.317373202554656	35.0	34.0	38.5	28.0	40.0
76-77	34.01998963816328	35.0	34.0	37.0	27.0	39.5
78-79	33.68971069039257	35.0	33.5	37.0	27.0	39.0
80-81	33.424357873587894	35.0	33.0	36.5	27.0	39.0
82-83	33.150569613669575	35.0	33.0	36.0	26.0	37.5
84-85	32.93286070024867	35.0	33.0	36.0	26.0	37.0
86-87	32.72472571017086	35.0	33.0	35.0	26.0	37.0
88-89	32.531950720644765	35.0	33.0	35.0	26.0	36.0
90-91	32.380335153407486	35.0	33.0	35.0	25.5	36.0
92-93	32.22014995066203	35.0	33.0	35.0	25.0	36.0
94-95	32.10387626369294	35.0	33.0	35.0	25.0	36.0
96-97	32.02046865041531	35.0	33.0	35.0	25.0	35.0
98-99	31.917153581040424	35.0	32.5	35.0	25.0	35.0
100	30.739218488781	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22737313005176674
1101	2	0.23372206899604464
1101	3	0.2389347624326632
1101	4	0.23693969317965013
1101	5	0.2469655424825703
1101	6	0.3445600954168384
1101	7	0.348953017146691
1101	8	0.3471336503440199
1101	9	0.420448371567808
1101	10-11	0.3691038294694948
1101	12-13	0.35641326084014935
1101	14-15	0.45040945188805637
1101	16-17	0.45416358707849724
1101	18-19	0.4659382431033663
1101	20-21	0.4530413487458702
1101	22-23	0.4431445065577151
1101	24-25	0.5166325856523741
1101	26-27	0.4739018125094674
1101	28-29	0.4961186074661583
1101	30-31	0.4919513651353711
1101	32-33	0.4970423698897548
1101	34-35	0.4127666645385091
1101	36-37	0.47443343899466583
1101	38-39	0.49181826613653357
1101	40-41	0.5044600446312373
1101	42-43	0.49646950130762235
1101	44-45	0.4289112344539632
1101	46-47	0.35370839413945987
1101	48-49	0.3438180337058725
1101	50-51	0.37665010210498195
1101	52-53	0.36698169743350206
1101	54-55	0.31277731576805934
1101	56-57	0.3545668108554594
1101	58-59	0.3888560442946485
1101	60-61	0.29744250968164465
1101	62-63	0.34271246481823425
1101	64-65	0.4528994218599536
1101	66-67	0.40457484082639183
1101	68-69	0.3822606744411843
1101	70-71	0.38252614127960527
1101	72-73	0.37116757599285677
1101	74-75	0.4016043582599096
1101	76-77	0.36119375962885414
1101	78-79	0.3404428812832023
1101	80-81	0.41525703758489385
1101	82-83	0.46975317916297143
1101	84-85	0.379657442900843
1101	86-87	0.3763102537379126
1101	88-89	0.34151461276218953
1101	90-91	0.4465604447208662
1101	92-93	0.3312343041118666
1101	94-95	0.3661238022540161
1101	96-97	0.3354635847799301
1101	98-99	0.4548006645531366
1101	100	0.4705142874168118
1102	1	0.2181561510388299
1102	2	0.2053110716843385
1102	3	0.24982231859164727
1102	4	0.28600640378310516
1102	5	0.2059020764416175
1102	6	0.3370248313710249
1102	7	0.3339230076800277
1102	8	0.34196786171691684
1102	9	0.4783066636299935
1102	10-11	0.41581815544864753
1102	12-13	0.4187684721666258
1102	14-15	0.5591642497325253
1102	16-17	0.5033347627623996
1102	18-19	0.4804029601424631
1102	20-21	0.5249511782906424
1102	22-23	0.4874635528990652
1102	24-25	0.5423344280064057
1102	26-27	0.49369273640468947
1102	28-29	0.5235945318896995
1102	30-31	0.49464057283159235
1102	32-33	0.5685014777397299
1102	34-35	0.4583409001083183
1102	36-37	0.50158074968855
1102	38-39	0.4277310996284598
1102	40-41	0.4462937582107429
1102	42-43	0.48058209565324717
1102	44-45	0.38841543722912775
1102	46-47	0.3676123074849045
1102	48-49	0.377387234404452
1102	50-51	0.358120176388006
1102	52-53	0.32864951796882735
1102	54-55	0.28122667711244276
1102	56-57	0.30222882138482987
1102	58-59	0.3655150359464159
1102	60-61	0.31488704993375194
1102	62-63	0.3338638260303952
1102	64-65	0.38299375200330843
1102	66-67	0.3188618184043719
1102	68-69	0.3035231685440323
1102	70-71	0.21819880110216872
1102	72-73	0.2419139065008764
1102	74-75	0.22337437694504558
1102	76-77	0.184455286181489
1102	78-79	0.14733392319612193
1102	80-81	0.10887526293921468
1102	82-83	0.17137325685214932
1102	84-85	0.196573483941215
1102	86-87	0.13388585637924422
1102	88-89	0.10925878200483652
1102	90-91	0.11468784622352501
1102	92-93	0.060177233196547775
1102	94-95	0.10426354705484897
1102	96-97	0.12037058421395841
1102	98-99	0.14164282199325484
1102	100	0.17069124915076372
1103	1	0.10068707743262095
1103	2	0.1350077989441303
1103	3	0.10494512332056516
1103	4	0.10419697541224338
1103	5	0.1234835391696194
1103	6	0.10789839680187896
1103	7	0.10744528914271001
1103	8	0.13847882718234672
1103	9	0.22089288145780728
1103	10-11	0.18356457566810747
1103	12-13	0.188202667742857
1103	14-15	0.27015170037413583
1103	16-17	0.24771181731901493
1103	18-19	0.2780387518814109
1103	20-21	0.2312883237620298
1103	22-23	0.2612851661846989
1103	24-25	0.26497707301870577
1103	26-27	0.2391567588861605
1103	28-29	0.25189583690803374
1103	30-31	0.33575843408120676
1103	32-33	0.35431178095772964
1103	34-35	0.33170670799864155
1103	36-37	0.3027618845541298
1103	38-39	0.2969640529947313
1103	40-41	0.20971317443500936
1103	42-43	0.21549326309069272
1103	44-45	0.19915393501898393
1103	46-47	0.2590964824671005
1103	48-49	0.1432676029349551
1103	50-51	0.22262776926753958
1103	52-53	0.19183968592761858
1103	54-55	0.07743254077768569
1103	56-57	0.23967052294884184
1103	58-59	0.26429125342302484
1103	60-61	0.10545708263332898
1103	62-63	0.14870208649857375
1103	64-65	0.04545585374373928
1103	66-67	0.11671766749643098
1103	68-69	0.08934519416636988
1103	70-71	0.11568279454674979
1103	72-73	0.05591563430117219
1103	74-75	0.0405331261234565
1103	76-77	0.052343219083716974
1103	78-79	0.05912789350148984
1103	80-81	-0.013464195996562012
1103	82-83	0.0013037480117716882
1103	84-85	0.006503500731170675
1103	86-87	0.009132836491758667
1103	88-89	-0.06720025822672682
1103	90-91	-0.007104491155487835
1103	92-93	-0.016044827456177302
1103	94-95	-0.04655259683972446
1103	96-97	0.010777711983152471
1103	98-99	-0.014071211549246954
1103	100	0.024414070391394205
1104	1	0.11450697886310479
1104	2	0.08111189985665845
1104	3	0.03722202771414018
1104	4	0.07199066172772461
1104	5	0.08031592592946168
1104	6	0.20422081396507963
1104	7	0.17399215901335197
1104	8	0.19967410597273982
1104	9	0.34104813810545664
1104	10-11	0.25465843127255994
1104	12-13	0.3015040814880834
1104	14-15	0.4029274600825019
1104	16-17	0.3542484311535077
1104	18-19	0.36197249960437716
1104	20-21	0.35943889009691077
1104	22-23	0.40363433486646016
1104	24-25	0.4118189433935697
1104	26-27	0.3652535432540702
1104	28-29	0.3858473932293407
1104	30-31	0.3967254195146239
1104	32-33	0.33638506655829303
1104	34-35	0.35068498794550607
1104	36-37	0.3626974732141335
1104	38-39	0.3344725013508736
1104	40-41	0.3870412195444004
1104	42-43	0.3231354046711914
1104	44-45	0.30631127505807143
1104	46-47	0.2787411902022612
1104	48-49	0.2631548723618806
1104	50-51	0.31329488365238234
1104	52-53	0.2742536950722325
1104	54-55	0.24806165633403054
1104	56-57	0.2625278589112625
1104	58-59	0.32629628703406865
1104	60-61	0.2630833466683242
1104	62-63	0.2920596938811997
1104	64-65	0.33602148057422454
1104	66-67	0.22117797041426002
1104	68-69	0.2565605301588363
1104	70-71	0.1832800498769913
1104	72-73	0.20524512984580667
1104	74-75	0.2703433250862659
1104	76-77	0.21042574799051295
1104	78-79	0.2175403106003202
1104	80-81	0.1632675829107626
1104	82-83	0.18085239659662733
1104	84-85	0.16601811987391102
1104	86-87	0.18275336744545712
1104	88-89	0.16511355073207312
1104	90-91	0.169281629295007
1104	92-93	0.12924790942500408
1104	94-95	0.18793740137942194
1104	96-97	0.18108831110302503
1104	98-99	0.24798145469830857
1104	100	0.2657939578324715
1105	1	0.12691051936262454
1105	2	0.0820465315493557
1105	3	0.15767098436060678
1105	4	0.15126275909857867
1105	5	0.11404663530007753
1105	6	0.1452113365110108
1105	7	0.17062732226892763
1105	8	0.19687509041621354
1105	9	0.26774328897924704
1105	10-11	0.2842067807235651
1105	12-13	0.3000220284700532
1105	14-15	0.37670053996534847
1105	16-17	0.3878428188904621
1105	18-19	0.34004860590881947
1105	20-21	0.3634740511160359
1105	22-23	0.3476224085025095
1105	24-25	0.3707923327194109
1105	26-27	0.3205339480103859
1105	28-29	0.325120222528426
1105	30-31	0.2791878090773281
1105	32-33	0.32131720562873056
1105	34-35	0.27350630698185086
1105	36-37	0.33430282564265923
1105	38-39	0.4096164218227969
1105	40-41	0.3298008736269793
1105	42-43	0.32585248492813434
1105	44-45	0.2538520470187109
1105	46-47	0.2654306686322343
1105	48-49	0.2602123175460491
1105	50-51	0.33533547923865115
1105	52-53	0.20618904547778527
1105	54-55	0.17014423613708374
1105	56-57	0.25421915191331834
1105	58-59	0.22294852861511316
1105	60-61	0.24009795371944165
1105	62-63	0.20510224839206614
1105	64-65	0.26095725895448396
1105	66-67	0.19666579549966912
1105	68-69	0.18759957344731504
1105	70-71	0.21477431186460905
1105	72-73	0.20881195845159795
1105	74-75	0.2468425326722894
1105	76-77	0.22643210356734755
1105	78-79	0.21109425930231396
1105	80-81	0.221100994011465
1105	82-83	0.18579067573168828
1105	84-85	0.24013418945995113
1105	86-87	0.21129816196168605
1105	88-89	0.16900751059597496
1105	90-91	0.15554343799133363
1105	92-93	0.15931297987817317
1105	94-95	0.22161850228081192
1105	96-97	0.21469774403145436
1105	98-99	0.1678340931471105
1105	100	0.23313938627987696
1106	1	0.12170630574382812
1106	2	0.16204263407619024
1106	3	0.15124628711352628
1106	4	0.1712712372672982
1106	5	0.07414237183709815
1106	6	0.14384873486226724
1106	7	0.10203361909488962
1106	8	0.15459290248968216
1106	9	0.17382571510194822
1106	10-11	0.18406617666180125
1106	12-13	0.19302260920908765
1106	14-15	0.30471853899331336
1106	16-17	0.28953806443902863
1106	18-19	0.2769040865169501
1106	20-21	0.2564622290395775
1106	22-23	0.28581756787189505
1106	24-25	0.3354938809945125
1106	26-27	0.2691882134143313
1106	28-29	0.2828007096970424
1106	30-31	0.2459675956324432
1106	32-33	0.3243371068099279
1106	34-35	0.2943958120648702
1106	36-37	0.27557850277039364
1106	38-39	0.22875225807923982
1106	40-41	0.17812365971471422
1106	42-43	0.3102519751510684
1106	44-45	0.28282494638160927
1106	46-47	0.2746443107199781
1106	48-49	0.2722249004838133
1106	50-51	0.31168775491267553
1106	52-53	0.2721050215463734
1106	54-55	0.2043931845180822
1106	56-57	0.2698941950417293
1106	58-59	0.23343377955183087
1106	60-61	0.23991847197467564
1106	62-63	0.25832410778179593
1106	64-65	0.3256786245607586
1106	66-67	0.3030311378018524
1106	68-69	0.27859375130562114
1106	70-71	0.21783967062623333
1106	72-73	0.27247588213565166
1106	74-75	0.28991079512906737
1106	76-77	0.2767803722973454
1106	78-79	0.24468121953722033
1106	80-81	0.2427415390676515
1106	82-83	0.2770056780530936
1106	84-85	0.25914038815033535
1106	86-87	0.15192103174415905
1106	88-89	0.13952330079700914
1106	90-91	0.1517697988849207
1106	92-93	0.20267663896883903
1106	94-95	0.2383294425345639
1106	96-97	0.1475298988061695
1106	98-99	0.1270150880741916
1106	100	0.21269461626961572
1107	1	0.12072963388655467
1107	2	0.14759856055693987
1107	3	0.12295872195805302
1107	4	0.03821990098580841
1107	5	0.07532795322360641
1107	6	0.19646884595671565
1107	7	0.12172578522524446
1107	8	0.12042813916225725
1107	9	0.1634660737819118
1107	10-11	0.20111435632294672
1107	12-13	0.24133828603370233
1107	14-15	0.25963224430358167
1107	16-17	0.26485610344674626
1107	18-19	0.29561046905961064
1107	20-21	0.29870223043406696
1107	22-23	0.3157087975804629
1107	24-25	0.34472974222997976
1107	26-27	0.29760426717407285
1107	28-29	0.27605182264868944
1107	30-31	0.31769883497567974
1107	32-33	0.21405994645826354
1107	34-35	0.2726651273486951
1107	36-37	0.22771762014215113
1107	38-39	0.21087728792345217
1107	40-41	0.16429905180964255
1107	42-43	0.20836790699849672
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2216	56-57	-0.3995520131328618
2216	58-59	-0.3802397398314241
2216	60-61	-0.41613102523724166
2216	62-63	-0.4885645055763348
2216	64-65	-0.4537331033837262
2216	66-67	-0.4301599583328226
2216	68-69	-0.5154729076950701
2216	70-71	-0.5521837356005435
2216	72-73	-0.3820120889469507
2216	74-75	-0.34237250770701877
2216	76-77	-0.45351869245349263
2216	78-79	-0.4141584432264622
2216	80-81	-0.42550769350141593
2216	82-83	-0.4266751573383871
2216	84-85	-0.4080224838578488
2216	86-87	-0.45319221639684315
2216	88-89	-0.39226071364169
2216	90-91	-0.40659244851281073
2216	92-93	-0.3895633927616373
2216	94-95	-0.4284490890642587
2216	96-97	-0.3827271697243262
2216	98-99	-0.33708355971417703
2216	100	-0.3587627025716884
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1051.0
1	272.0
2	188.0
3	170.0
4	149.0
5	210.0
6	302.0
7	631.0
8	1893.0
9	2897.0
10	3637.0
11	4472.0
12	5826.0
13	7161.0
14	8774.0
15	9331.0
16	10187.0
17	11128.0
18	12074.0
19	13250.0
20	14779.0
21	16225.0
22	18090.0
23	20441.0
24	24536.0
25	28962.0
26	32564.0
27	32997.0
28	36049.0
29	44047.0
30	51995.0
31	61219.0
32	77024.0
33	102815.0
34	149128.0
35	224325.0
36	399677.0
37	792525.0
38	976496.0
39	282070.0
40	511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.522985875841904	19.74526066861884	13.325954920158111	24.405798535381148
2	14.598497331902987	21.081258570070215	40.323443686749684	23.996800411277114
3	18.49944327117295	26.115671916481475	30.06628983946885	25.318594972876724
4	12.120981188462196	17.70658211146815	39.623688722452314	30.548747977617335
5	13.020727139395943	38.417634244713966	35.42786480987541	13.13377380601468
6	29.7931673168985	38.11907798899327	18.89668144488081	13.191073249227415
7	26.447178720845262	32.56898794481482	23.217152949317146	17.766680385022777
8	25.237387460972283	35.3633115291963	21.197055176047506	18.202245833783913
9	26.104215336800202	16.267643462587404	20.873807905237452	36.75433329537494
10-11	24.086927388439783	26.607065312730967	29.1079064898613	20.198100808967958
12-13	25.01903962643369	24.360226137708437	28.42592862839495	22.194805607462932
14-15	22.64190553343833	25.745818348666333	26.0824440310016	25.529832086893745
16-17	22.237146167219855	28.460090925025604	26.93360189724066	22.36916101051388
18-19	22.07798855796644	28.629025126489445	27.71976206022748	21.57322425531664
20-21	23.13433758585439	27.66664414119731	27.117463612979847	22.081554659968454
22-23	22.884485674712206	27.614639470563716	26.665664011788	22.83521084293608
24-25	22.760281887839284	27.792532827889588	26.874230464344805	22.57295481992632
26-27	22.80069053876488	27.633656734025397	27.02014004300986	22.545512684199863
28-29	22.5028628846489	28.030804121173496	27.074692674868906	22.3916403193087
30-31	22.96157387455439	27.629788094994666	26.904126009587586	22.50451202086336
32-33	22.638247621983872	27.934362901050257	26.83771740362111	22.58967207334476
34-35	22.927631058606977	27.876631267121567	27.04245788364886	22.153279790622594
36-37	22.902852242033394	27.59928805756397	27.133148967641763	22.36471073276087
38-39	22.78574995977039	27.73001594947707	27.097932895426162	22.38630119532638
40-41	22.822337705738864	27.57323224443328	27.14873709096865	22.4556929588592
42-43	22.760755791253573	27.56963575893705	26.88591529223427	22.783693157575104
44-45	22.791546645349356	27.648410484193715	26.980188160063317	22.57985471039361
46-47	22.706135115504804	27.665474152143453	27.053010887069462	22.575379845282274
48-49	22.809488562244006	27.479189535539394	27.13355527135396	22.577766630862634
50-51	22.37432691826497	27.677745060424204	27.141551799611037	22.806376221699793
52-53	22.373924367203976	27.841097927436177	26.709662017425845	23.075315687934
54-55	22.458970565565416	27.620350323380737	26.86107858002379	23.059600531030057
56-57	22.89500891129732	27.261494264857877	26.86527841314624	22.978218410698563
58-59	22.82180603628606	27.251922087070373	27.180459091857635	22.745812784785933
60-61	22.7196165312647	27.50309562125991	27.158298781741802	22.618989065733594
62-63	22.43061124463707	27.399785540308415	27.196184021142226	22.97341919391229
64-65	22.111905828912786	27.683740219515464	27.10578484631099	23.098569105260765
66-67	22.678045525887995	27.293154001913706	26.816914283610366	23.21188618858794
68-69	22.56676119796015	27.202735180209896	26.980105570730707	23.250398051099246
70-71	22.611884981011286	27.127769888386123	27.04062862519304	23.219716505409547
72-73	22.7745375775498	27.022536433915878	27.37005617785233	22.832869810681988
74-75	22.595884732942558	27.425213964087053	27.101427347708253	22.877473955262133
76-77	22.47094507428943	27.1370776950502	27.18066907227022	23.211308158390153
78-79	22.503188667272276	27.13721250935601	27.24594649482478	23.11365232854693
80-81	22.537215635097077	27.082854531904065	27.26441315747766	23.115516675521196
82-83	22.55179886888052	26.912960295756015	27.61436540722401	22.920875428139453
84-85	22.6533368061693	27.087085005330785	27.152236635322453	23.107341553177466
86-87	22.39232591836221	27.151910430696326	27.3027458284046	23.15301782253686
88-89	22.626228836740527	26.782327416355113	27.24170483066804	23.34973891623632
90-91	22.748871260894415	26.7305583192178	27.268099015522623	23.252471404365156
92-93	22.764264587939067	26.916072945483588	27.27047210990458	23.04919035667276
94-95	22.322487996029764	26.818844705317613	27.469933090691757	23.388734207960866
96-97	22.38937325170652	26.727801454258053	27.533533584778898	23.34929170925653
98-99	22.59120836321284	26.83934792344978	27.17182665254887	23.39761706078851
100	22.709041699456506	26.619510750650132	27.04130425013913	23.630143299754238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1590.0
1	1036.0
2	488.5
3	510.5
4	491.5
5	407.0
6	367.0
7	363.0
8	398.0
9	444.0
10	399.5
11	364.0
12	380.5
13	426.0
14	461.0
15	502.5
16	602.5
17	747.0
18	933.5
19	1231.0
20	1673.0
21	2374.0
22	3568.5
23	5189.0
24	7101.5
25	9958.0
26	14183.0
27	19277.5
28	25136.5
29	32166.0
30	39940.5
31	46916.0
32	55054.0
33	63993.0
34	71554.5
35	78502.5
36	86366.0
37	95653.0
38	101367.0
39	104327.5
40	106958.0
41	107980.5
42	110170.5
43	115020.0
44	120908.5
45	128512.5
46	137622.0
47	149099.5
48	160840.0
49	161014.5
50	158246.5
51	149972.5
52	133451.0
53	123869.0
54	114661.5
55	102029.5
56	91110.5
57	80186.5
58	69273.5
59	61047.5
60	52223.5
61	42275.5
62	33080.0
63	25559.0
64	18877.5
65	13387.0
66	9685.5
67	6742.0
68	4697.5
69	3499.0
70	2746.5
71	2167.5
72	1734.5
73	1481.5
74	1180.0
75	894.5
76	671.5
77	453.5
78	298.0
79	186.0
80	114.5
81	81.0
82	56.5
83	46.5
84	46.0
85	40.5
86	32.5
87	26.0
88	20.5
89	16.0
90	13.5
91	10.5
92	12.5
93	17.0
94	12.0
95	9.0
96	11.0
97	11.0
98	9.0
99	7.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09393467617679833
2	0.061234259691880476
3	0.0753431388606807
4	0.0788488074117879
5	0.07910742230490236
6	0.08945201802948094
7	0.08577393954963078
8	0.08798653363516565
9	0.07430867928822285
10-11	0.08068784665171297
12-13	0.07075990825492992
14-15	0.07969648956144086
16-17	0.08096082903888936
18-19	0.08078841911014638
20-21	0.07278572491765989
22-23	0.07596094110534304
24-25	0.078662029988983
26-27	0.07193804276800693
28-29	0.08091772655670361
30-31	0.08555842713870207
32-33	0.08677966413396482
34-35	0.08916466814824266
36-37	0.0894089155472952
38-39	0.09084566495348667
40-41	0.08639174179429313
42-43	0.09248355927654495
44-45	0.08883421578481862
46-47	0.08538601720995909
48-49	0.0942938635283462
50-51	0.09080256247130093
52-53	0.09613290276827129
54-55	0.08798653363516565
56-57	0.09515591317206108
58-59	0.09923628148564487
60-61	0.09749781470415318
62-63	0.09867594921723019
64-65	0.10091727829088888
66-67	0.10265574507238057
68-69	0.09917881150939721
70-71	0.10169312297023227
72-73	0.098877094134097
74-75	0.1022965577208327
76-77	0.09979661375405953
78-79	0.10643439601066412
80-81	0.10100348325526036
82-83	0.10629072107004497
84-85	0.10265574507238057
86-87	0.09886272664003508
88-89	0.09815871943100127
90-91	0.09801504449038212
92-93	0.09216747440718283
94-95	0.09216747440718283
96-97	0.08953822299385245
98-99	0.10387698206764331
100	0.2435002893613304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3480078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.13495016025556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12212142990788	47.53818563679552
2	14.674547246217164	18.823027125209066
3	5.244428193530033	10.090534224332634
4	2.4711277688594766	6.339426251817043
5	1.2691402157708862	4.069812224242089
6	0.7012457079543941	2.6984615117848914
7	0.4464856217915767	2.004473317061144
8	0.2948711085003236	1.5129235077894054
9	0.19537941283987234	1.1277584014342688
>10	0.5721309279574786	5.318700580437788
>50	0.006751512468674694	0.28245848849260824
>100	0.001705533577813584	0.1510885801770505
>500	0.0	0.0
>1k	6.532062443951148E-5	0.043150150426507855
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.873498812382941E-5
2	0.0	0.0	0.0	0.0	2.873498812382941E-5
3	0.0	0.0	0.0	2.873498812382941E-5	2.873498812382941E-5
4	0.0	0.0	0.0	2.873498812382941E-5	2.873498812382941E-5
5	0.0	0.0	0.0	2.873498812382941E-5	2.873498812382941E-5
6	0.0	0.0	0.0	2.873498812382941E-5	2.873498812382941E-5
7	0.0	0.0	0.0	5.746997624765882E-5	2.873498812382941E-5
8	0.0	0.0	0.0	5.746997624765882E-5	2.873498812382941E-5
9	0.0	0.0	0.0	1.7240992874297645E-4	2.873498812382941E-5
10-11	0.0	0.0	0.0	1.7240992874297645E-4	2.873498812382941E-5
12-13	0.0	0.0	0.0	2.0114491686680586E-4	2.873498812382941E-5
14-15	0.0	0.0	0.0	2.873498812382941E-4	4.3102482185744114E-5
16-17	0.0	0.0	0.0	3.591873515478676E-4	5.746997624765882E-5
18-19	0.0	0.0	0.0	3.735548456097823E-4	8.620496437148823E-5
20-21	0.0	0.0	0.0	4.7412730404318524E-4	8.620496437148823E-5
22-23	0.0	0.0	0.0	6.465372327861616E-4	8.620496437148823E-5
24-25	1.4367494061914705E-5	0.0	0.0	0.0011781345130770057	8.620496437148823E-5
26-27	2.873498812382941E-5	0.0	0.0	0.0019108767102346555	8.620496437148823E-5
28-29	2.873498812382941E-5	0.0	0.0	0.004540128123565046	8.620496437148823E-5
30-31	2.873498812382941E-5	0.0	0.0	0.013031317114156638	8.620496437148823E-5
32-33	2.873498812382941E-5	0.0	0.0	0.03239869910961766	8.620496437148823E-5
34-35	4.3102482185744114E-5	0.0	0.0	0.06348995625960108	8.620496437148823E-5
36-37	5.746997624765882E-5	0.0	0.0	0.11202335120074894	8.620496437148823E-5
38-39	5.746997624765882E-5	0.0	0.0	0.18705040519206753	8.620496437148823E-5
40-41	5.746997624765882E-5	0.0	0.0	0.273039857152627	8.620496437148823E-5
42-43	5.746997624765882E-5	0.0	0.0	0.35931665899442483	1.0057245843340293E-4
44-45	5.746997624765882E-5	0.0	0.0	0.46575105500508895	1.2930744655723233E-4
46-47	5.746997624765882E-5	0.0	0.0	0.5961647986050888	1.4367494061914705E-4
48-49	5.746997624765882E-5	0.0	0.0	0.7352277736303612	1.4367494061914705E-4
50-51	5.746997624765882E-5	0.0	0.0	0.8805406085725664	1.7240992874297645E-4
52-53	5.746997624765882E-5	0.0	0.0	1.0303504691561511	1.7240992874297645E-4
54-55	5.746997624765882E-5	0.0	0.0	1.1970277677684236	2.0114491686680586E-4
56-57	5.746997624765882E-5	0.0	0.0	1.3953135533169085	2.0114491686680586E-4
58-59	5.746997624765882E-5	0.0	0.0	1.617233866597243	2.0114491686680586E-4
60-61	5.746997624765882E-5	0.0	0.0	1.8516251647233193	2.2987990499063527E-4
62-63	5.746997624765882E-5	0.0	0.0	2.089364088965822	2.2987990499063527E-4
64-65	5.746997624765882E-5	0.0	0.0	2.3432520765339167	2.2987990499063527E-4
66-67	5.746997624765882E-5	0.0	0.0	2.631866297249659	2.2987990499063527E-4
68-69	5.746997624765882E-5	0.0	0.0	2.9568733804242315	2.2987990499063527E-4
70-71	5.746997624765882E-5	0.0	0.0	3.3135607880053266	2.2987990499063527E-4
72-73	5.746997624765882E-5	0.0	0.0	3.6687539762039814	2.2987990499063527E-4
74-75	5.746997624765882E-5	0.0	0.0	4.023702917003584	2.2987990499063527E-4
76-77	5.746997624765882E-5	0.0	0.0	4.4181768339675145	2.873498812382941E-4
78-79	5.746997624765882E-5	0.0	0.0	4.8587129368939435	2.873498812382941E-4
80-81	5.746997624765882E-5	0.0	0.0	5.337840128870674	3.448198574859529E-4
82-83	5.746997624765882E-5	0.0	0.0	5.82989806550313	4.5975980998127055E-4
84-85	5.746997624765882E-5	0.0	0.0	6.320116962895659	4.5975980998127055E-4
86-87	5.746997624765882E-5	0.0	0.0	6.817217889943846	4.7412730404318524E-4
88	5.746997624765882E-5	0.0	0.0	7.217338232074109	4.884947981050999E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	6735	0.0	18.28689	4
GTGATCG	745	0.0	17.66177	8
ACATGGG	15935	0.0	17.434584	3
GGCGTCG	755	0.0	17.427837	8
TACATGG	17365	0.0	17.352644	2
GTACTAG	1065	0.0	17.218336	1
GTATATA	2360	0.0	16.53645	1
GTATAAG	2015	0.0	16.334248	1
GTACATG	19850	0.0	16.273178	1
CTAGTAC	1545	0.0	15.821623	3
GTACCCG	485	2.910383E-11	15.51152	1
GAACCGT	455	1.8735591E-10	15.492395	6
ATGGGGG	4310	0.0	15.269575	5
TTAGGGC	2990	0.0	15.250241	3
CGGGCGT	835	0.0	15.195536	6
TGATCGC	885	0.0	14.866749	9
TCTAACG	555	1.6370905E-11	14.3992	2
CTAACGC	490	5.711627E-10	14.39035	3
CTGTGCG	1440	0.0	14.357916	9
GTATTAT	1625	0.0	14.178127	1
>>END_MODULE