FastQCFastQC Report
Mon 16 Jan 2017
ERR1146621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480078
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81140.23315569363675182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76920.22102952864849584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40000.11493995249531763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC4750.021.7806153
GAGTACT96100.021.64057512-13
TAACGCC5650.020.8081114
GTACTTT101750.020.20796414-15
TCTAACG5150.020.0889192
AGTACTT101700.018.57736212-13
GTCGACG3750.017.5456536-37
TATACTG16000.017.332245
CATGGGG65350.017.1986124
CTGTGCG14500.016.8541979
ACTTTTT124550.016.81046716-17
TACCTGG29500.016.7381842
GTACTAG11450.016.428651
TAGTACT18050.016.4136224
ACATGGG157650.016.4062673
TCGACGA4050.016.24597436-37
TACATGG173200.016.2094212
GTCTTAT11750.016.0091931
CGGGCGT9200.015.8374046
CTAGTAC15750.015.8247333
Produced by FastQC (version 0.11.5)