##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146621_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3480078
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.251449249126026	33.0	31.0	34.0	30.0	34.0
2	32.361170927778055	34.0	31.0	34.0	30.0	34.0
3	32.51215806082507	34.0	31.0	34.0	31.0	34.0
4	36.0330912123234	37.0	35.0	37.0	35.0	37.0
5	36.05018651880791	37.0	35.0	37.0	35.0	37.0
6	36.12677273325483	37.0	36.0	37.0	35.0	37.0
7	36.10269769815504	37.0	36.0	37.0	35.0	37.0
8	36.10702375061708	37.0	36.0	37.0	35.0	37.0
9	37.895366713044936	39.0	38.0	39.0	35.0	39.0
10-11	37.847414339563656	39.0	38.0	39.0	35.0	39.0
12-13	37.820238080870595	39.0	38.0	39.0	35.0	39.0
14-15	39.18776978561975	40.0	39.0	41.0	36.0	41.0
16-17	39.12441071723105	40.0	39.0	41.0	36.0	41.0
18-19	39.219198391530306	40.0	39.0	41.0	36.0	41.0
20-21	39.277146230630464	40.5	39.0	41.0	36.0	41.0
22-23	39.24870721288431	40.5	39.0	41.0	36.0	41.0
24-25	39.243068833514656	41.0	39.0	41.0	36.0	41.0
26-27	39.17335315472814	40.5	39.0	41.0	36.0	41.0
28-29	39.11732754265853	40.0	39.0	41.0	35.5	41.0
30-31	39.001596515940165	40.0	39.0	41.0	35.0	41.0
32-33	38.917124702377365	40.0	38.0	41.0	35.0	41.0
34-35	38.86272520328568	40.0	38.0	41.0	35.0	41.0
36-37	38.80903660780017	40.0	38.0	41.0	35.0	41.0
38-39	38.74074819587377	40.0	38.0	41.0	35.0	41.0
40-41	38.66816203544863	40.0	38.0	41.0	35.0	41.0
42-43	38.5843646320571	40.0	38.0	41.0	35.0	41.0
44-45	38.475749106772895	40.0	38.0	41.0	34.0	41.0
46-47	38.28236795267233	40.0	38.0	41.0	34.0	41.0
48-49	38.038944529404226	40.0	38.0	41.0	33.0	41.0
50-51	37.94569575739394	40.0	37.5	41.0	33.0	41.0
52-53	37.864036093443886	40.0	37.0	41.0	33.0	41.0
54-55	37.71792428215689	40.0	37.0	41.0	33.0	41.0
56-57	37.57347680138204	40.0	37.0	41.0	33.0	41.0
58-59	37.40927013704865	40.0	36.0	41.0	33.0	41.0
60-61	37.20598977953942	39.0	36.0	41.0	32.0	41.0
62-63	36.97434727037728	39.0	35.0	41.0	32.0	41.0
64-65	36.711668818917275	39.0	35.0	41.0	32.0	41.0
66-67	36.43810885847961	38.0	35.0	40.0	31.0	41.0
68-69	36.13823095344415	37.0	35.0	40.0	31.0	41.0
70-71	35.80780962380728	37.0	35.0	39.5	31.0	41.0
72-73	35.46601153767243	36.0	35.0	39.0	31.0	41.0
74-75	35.13194258864313	36.0	35.0	39.0	31.0	40.5
76-77	34.806651603785895	35.0	35.0	37.5	30.0	39.5
78-79	34.500274562811526	35.0	34.0	37.0	30.0	39.0
80-81	34.22242475599685	35.0	34.0	37.0	30.0	39.0
82-83	33.96118362864281	35.0	34.0	36.0	30.0	38.0
84-85	33.73734985250331	35.0	34.0	36.0	30.0	37.0
86-87	33.54265550944548	35.0	34.0	35.5	30.0	37.0
88-89	33.339175012743965	35.0	34.0	35.0	29.0	36.0
90-91	33.247368737137506	35.0	34.0	35.0	29.0	36.0
92-93	33.13994470813586	35.0	34.0	35.0	29.0	36.0
94-95	33.064259622916495	35.0	34.0	35.0	29.0	36.0
96-97	33.01164399188754	35.0	34.0	35.0	29.0	35.5
98-99	32.944186452142745	35.0	34.0	35.0	29.0	35.0
100	32.20391927997016	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2928107540519207
1101	2	0.325408095198064
1101	3	0.30194634971640966
1101	4	0.26085942109963867
1101	5	0.23179548083923862
1101	6	0.26782376216749526
1101	7	0.26415755147175446
1101	8	0.2653938749209175
1101	9	0.32703097858937014
1101	10-11	0.3221635736040511
1101	12-13	0.31930746153371103
1101	14-15	0.49707621049604
1101	16-17	0.49120112513184466
1101	18-19	0.4826878731127593
1101	20-21	0.4331424145261309
1101	22-23	0.41150973906881916
1101	24-25	0.4233755294200279
1101	26-27	0.40674716129134225
1101	28-29	0.4719967586597349
1101	30-31	0.49099414408242126
1101	32-33	0.48756835603939663
1101	34-35	0.4747486627662667
1101	36-37	0.5038941210828654
1101	38-39	0.5489545894747536
1101	40-41	0.5367072541454263
1101	42-43	0.48966449099061293
1101	44-45	0.5393692496173799
1101	46-47	0.5263474174156926
1101	48-49	0.6104383539427332
1101	50-51	0.5304985933769686
1101	52-53	0.4731213831327423
1101	54-55	0.5343654881294029
1101	56-57	0.46165448812793386
1101	58-59	0.48255940462509983
1101	60-61	0.44514543692639563
1101	62-63	0.4696947628178876
1101	64-65	0.44252199695601036
1101	66-67	0.428573366862544
1101	68-69	0.38706709564399944
1101	70-71	0.4430391931772064
1101	72-73	0.4290712653808484
1101	74-75	0.4268605186984402
1101	76-77	0.42473856219405093
1101	78-79	0.37815046873335234
1101	80-81	0.38815420942930245
1101	82-83	0.4073549152415552
1101	84-85	0.35050594450443384
1101	86-87	0.3757062930388315
1101	88-89	0.4753924944226995
1101	90-91	0.3879513519943316
1101	92-93	0.40075222730229854
1101	94-95	0.4246279446637331
1101	96-97	0.3522277218769787
1101	98-99	0.38928704653794455
1101	100	0.42530652359499044
1102	1	0.21421724769925987
1102	2	0.25080116066583713
1102	3	0.24455234156650363
1102	4	0.2165163468296214
1102	5	0.18771083282294398
1102	6	0.20059621019383655
1102	7	0.18527791833621876
1102	8	0.2518184072776606
1102	9	0.2732168677669975
1102	10-11	0.2788147659005915
1102	12-13	0.32878485912613087
1102	14-15	0.40282446395847415
1102	16-17	0.5099771630891539
1102	18-19	0.4264697932318313
1102	20-21	0.3869090551869405
1102	22-23	0.3537459984661666
1102	24-25	0.3827409174984382
1102	26-27	0.33186230931850247
1102	28-29	0.3338983390157253
1102	30-31	0.4168070862017146
1102	32-33	0.36707763132394433
1102	34-35	0.36896617490636885
1102	36-37	0.4015168299862637
1102	38-39	0.39979177675822086
1102	40-41	0.40279449487363905
1102	42-43	0.46242718958288975
1102	44-45	0.35143085693842124
1102	46-47	0.46844529418693526
1102	48-49	0.5779700212404677
1102	50-51	0.4777766131223089
1102	52-53	0.4956300313867672
1102	54-55	0.49464737005822457
1102	56-57	0.44314150918182094
1102	58-59	0.4216760179478456
1102	60-61	0.4110297388733102
1102	62-63	0.4553946646946443
1102	64-65	0.4465891499517909
1102	66-67	0.4533755181134822
1102	68-69	0.4117221268402176
1102	70-71	0.43418659374178503
1102	72-73	0.458080824559822
1102	74-75	0.41520046823354306
1102	76-77	0.4057476651064107
1102	78-79	0.40955521153441765
1102	80-81	0.3644851154377591
1102	82-83	0.34631293463355206
1102	84-85	0.33357422410042403
1102	86-87	0.3318684053688514
1102	88-89	0.3722155365773858
1102	90-91	0.2506670014538841
1102	92-93	0.34283104495730043
1102	94-95	0.36638707804907256
1102	96-97	0.3998039807856131
1102	98-99	0.39619720037573813
1102	100	0.4623834178155022
1103	1	0.16852399975854837
1103	2	0.167822182996332
1103	3	0.2152233876382894
1103	4	0.18420917239888013
1103	5	0.15800128845957317
1103	6	0.16733058096755826
1103	7	0.2002289556321628
1103	8	0.16014058456146074
1103	9	0.19801686849679356
1103	10-11	0.22109652050278328
1103	12-13	0.2435474941219269
1103	14-15	0.34884055694952565
1103	16-17	0.4349915880322399
1103	18-19	0.3276964949531944
1103	20-21	0.35012241708584924
1103	22-23	0.35965146753795096
1103	24-25	0.30733791993824866
1103	26-27	0.299072805842151
1103	28-29	0.3143182975722354
1103	30-31	0.3350246147995861
1103	32-33	0.3216845818430798
1103	34-35	0.33626078082149036
1103	36-37	0.23970329193200968
1103	38-39	0.2250414023938916
1103	40-41	0.2529063138395671
1103	42-43	0.247284807982588
1103	44-45	0.22632627895181656
1103	46-47	0.2426529714039205
1103	48-49	0.30201369882782103
1103	50-51	0.24159757871089482
1103	52-53	0.19397233745281284
1103	54-55	0.22799589994556158
1103	56-57	0.17774246622263234
1103	58-59	0.1877543673968205
1103	60-61	0.16952503828039056
1103	62-63	0.17273057269976988
1103	64-65	0.18502698221011116
1103	66-67	0.20939999011495303
1103	68-69	0.19621044389066356
1103	70-71	0.1698427496233066
1103	72-73	0.15805507632148164
1103	74-75	0.17012401489487416
1103	76-77	0.14339763320049315
1103	78-79	0.16846074948102796
1103	80-81	0.15950323042952874
1103	82-83	0.10537157082988102
1103	84-85	0.1078035216273534
1103	86-87	0.11066376183713089
1103	88-89	0.21999596545317956
1103	90-91	0.14724344361749786
1103	92-93	0.1755221395333777
1103	94-95	0.16831785383050146
1103	96-97	0.11952186310818291
1103	98-99	0.1891508494416172
1103	100	0.16747390611065782
1104	1	0.1847321844186922
1104	2	0.2057508499047387
1104	3	0.20287602127250892
1104	4	0.17861662883754548
1104	5	0.1267793825962542
1104	6	0.19665640751181002
1104	7	0.20143264523044024
1104	8	0.2067302379803948
1104	9	0.277047136677119
1104	10-11	0.2215947321332976
1104	12-13	0.22397745376292733
1104	14-15	0.32449706127185607
1104	16-17	0.38677791918205173
1104	18-19	0.29919994831934815
1104	20-21	0.27696748442594554
1104	22-23	0.27389587874921517
1104	24-25	0.26783619593719266
1104	26-27	0.2662154055112964
1104	28-29	0.3025919792532008
1104	30-31	0.3361755222871352
1104	32-33	0.3032484986189985
1104	34-35	0.3162202072558884
1104	36-37	0.3288337173858622
1104	38-39	0.2613388660632765
1104	40-41	0.2405141041358192
1104	42-43	0.2910649940931691
1104	44-45	0.28152338580555636
1104	46-47	0.2995416805380131
1104	48-49	0.4002338045207807
1104	50-51	0.3699177558528106
1104	52-53	0.3861811829621473
1104	54-55	0.3862588750113858
1104	56-57	0.42374282668583163
1104	58-59	0.38023302389899527
1104	60-61	0.3348416129359606
1104	62-63	0.35964822617020076
1104	64-65	0.3286491438306527
1104	66-67	0.31673927824305537
1104	68-69	0.30526354140668843
1104	70-71	0.3558247007161768
1104	72-73	0.31652403035598553
1104	74-75	0.35489777059952843
1104	76-77	0.35688893380748965
1104	78-79	0.3332473574150754
1104	80-81	0.24256403499196466
1104	82-83	0.2505267738371728
1104	84-85	0.33905922091522456
1104	86-87	0.28564504135273694
1104	88-89	0.33971652065232405
1104	90-91	0.3097313477171326
1104	92-93	0.31484781588464017
1104	94-95	0.34315516262945067
1104	96-97	0.30799143937083073
1104	98-99	0.30589801240316206
1104	100	0.3568750008030577
1105	1	0.12992458497048887
1105	2	0.13388505301278997
1105	3	0.10600206643044885
1105	4	0.12371448865905421
1105	5	0.08288869607807214
1105	6	0.10532619910541285
1105	7	0.08978207590210019
1105	8	0.118715054535798
1105	9	0.12500083815002228
1105	10-11	0.1301497533134679
1105	12-13	0.18025653393245022
1105	14-15	0.30865132327980405
1105	16-17	0.31760084032796954
1105	18-19	0.2080830156679241
1105	20-21	0.24888095830983303
1105	22-23	0.1915914636971081
1105	24-25	0.1854675338511811
1105	26-27	0.19007687589412114
1105	28-29	0.19224678359963576
1105	30-31	0.2616682777224639
1105	32-33	0.24856685182113125
1105	34-35	0.19781881984525995
1105	36-37	0.19904169682681072
1105	38-39	0.22930938155423064
1105	40-41	0.21806596684234592
1105	42-43	0.2523425341637022
1105	44-45	0.2659477496633471
1105	46-47	0.29245803842245266
1105	48-49	0.42600398108830007
1105	50-51	0.3617586875161294
1105	52-53	0.31264150686072867
1105	54-55	0.3639776580180367
1105	56-57	0.33819037843671396
1105	58-59	0.28980064746017575
1105	60-61	0.2926051858843053
1105	62-63	0.28257933444169936
1105	64-65	0.20331588775641052
1105	66-67	0.2060884008949202
1105	68-69	0.23206004400960012
1105	70-71	0.2586197516614348
1105	72-73	0.18781343376331705
1105	74-75	0.2161970652036942
1105	76-77	0.24696063227745668
1105	78-79	0.2069164424946166
1105	80-81	0.17928975403521008
1105	82-83	0.1753292802847497
1105	84-85	0.09064725104220628
1105	86-87	0.15547811088693209
1105	88-89	0.2050780169576143
1105	90-91	0.14822192871565676
1105	92-93	0.1578621199987893
1105	94-95	0.1492532430459974
1105	96-97	0.10503373770681179
1105	98-99	0.1006269092348333
1105	100	0.03117486570130268
1106	1	0.052307260935187117
1106	2	0.07947322208548968
1106	3	0.10302453668310108
1106	4	0.0962563632038993
1106	5	0.08933373471895578
1106	6	0.12863634946091906
1106	7	0.11398332682017553
1106	8	0.10498504073159864
1106	9	0.1527357851304174
1106	10-11	0.15588245109470478
1106	12-13	0.12005266580679574
1106	14-15	0.24953150781196598
1106	16-17	0.31446121995117693
1106	18-19	0.20996618619246732
1106	20-21	0.20912812953623217
1106	22-23	0.17310911412637608
1106	24-25	0.1298148390823286
1106	26-27	0.16889362400400643
1106	28-29	0.16558241892658287
1106	30-31	0.21379317698598754
1106	32-33	0.1562761236147665
1106	34-35	0.18067024847879765
1106	36-37	0.17660824501650296
1106	38-39	0.1401504321949858
1106	40-41	0.1707211786645786
1106	42-43	0.1916140211195838
1106	44-45	0.1386572077903523
1106	46-47	0.19255002111829356
1106	48-49	0.32951576356892787
1106	50-51	0.22202788878257707
1106	52-53	0.20101154786959796
1106	54-55	0.252025225823985
1106	56-57	0.19117856412413659
1106	58-59	0.22264984397511967
1106	60-61	0.16454893028459594
1106	62-63	0.1882968771081508
1106	64-65	0.2738819685598841
1106	66-67	0.2432930430804774
1106	68-69	0.2104248754477709
1106	70-71	0.1810501561446145
1106	72-73	0.20198010819387235
1106	74-75	0.18016939003867094
1106	76-77	0.16118246915188905
1106	78-79	0.09134582369699729
1106	80-81	0.1640853579649857
1106	82-83	0.12904399146025014
1106	84-85	0.13213645457570777
1106	86-87	0.05503943164897862
1106	88-89	0.15990515781167147
1106	90-91	0.10504464182281481
1106	92-93	0.09558556477500701
1106	94-95	0.11028031650948833
1106	96-97	0.06941399677231175
1106	98-99	0.09092435553587563
1106	100	0.05548415115937644
1107	1	0.049960924326207135
1107	2	0.07355297428868113
1107	3	0.09192724136161701
1107	4	0.045716895941687596
1107	5	0.054255421376844026
1107	6	0.06353369591373337
1107	7	0.057889137410278124
1107	8	0.05170941326350942
1107	9	0.120209284520854
1107	10-11	0.09967033740566222
1107	12-13	0.11664474657739277
1107	14-15	0.13901889506419707
1107	16-17	0.2504682167037018
1107	18-19	0.1801011031885693
1107	20-21	0.15805067401732487
1107	22-23	0.15644032579108114
1107	24-25	0.15302069974733
1107	26-27	0.14877769638230376
1107	28-29	0.11496702057757346
1107	30-31	0.17406391951013944
1107	32-33	0.13626068913576717
1107	34-35	0.09315643562370468
1107	36-37	0.13050024803366966
1107	38-39	0.12356430970375243
1107	40-41	0.08603094580572446
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2216	36-37	-0.5114298315566046
2216	38-39	-0.5087130450920512
2216	40-41	-0.5003071787832525
2216	42-43	-0.521545336204916
2216	44-45	-0.4810011896205424
2216	46-47	-0.44409663901562624
2216	48-49	-0.45606874215889803
2216	50-51	-0.4122131545235277
2216	52-53	-0.4905949575449142
2216	54-55	-0.4565826661324124
2216	56-57	-0.45535262306901814
2216	58-59	-0.45953563433183575
2216	60-61	-0.5328178900349059
2216	62-63	-0.5076186154975133
2216	64-65	-0.4663072477439911
2216	66-67	-0.5210065032936839
2216	68-69	-0.4822777813131154
2216	70-71	-0.4286272645541871
2216	72-73	-0.38695667916193344
2216	74-75	-0.36644766442642407
2216	76-77	-0.414639248560178
2216	78-79	-0.37117109144730875
2216	80-81	-0.4163226136509053
2216	82-83	-0.3785636571847064
2216	84-85	-0.3585408428008563
2216	86-87	-0.24813474100881905
2216	88-89	-0.2210490979719708
2216	90-91	-0.24585919349867424
2216	92-93	-0.2923663945486581
2216	94-95	-0.2645844873248393
2216	96-97	-0.28303157479291485
2216	98-99	-0.3466760520940966
2216	100	-0.3439240687674925
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	21.0
8	122.0
9	226.0
10	234.0
11	155.0
12	151.0
13	217.0
14	385.0
15	663.0
16	1203.0
17	2103.0
18	3655.0
19	5371.0
20	7956.0
21	11507.0
22	16718.0
23	20002.0
24	19565.0
25	18679.0
26	18349.0
27	21505.0
28	26413.0
29	32782.0
30	41807.0
31	53388.0
32	68140.0
33	89468.0
34	128117.0
35	194983.0
36	348935.0
37	794077.0
38	1159699.0
39	392424.0
40	1056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.90399041835226	19.876171915634146	13.626635279567218	24.593202386446382
2	14.86326031546249	21.150268536797732	39.588588407371326	24.397882740368452
3	18.13046863999862	26.045338168485117	30.697572175272335	25.126621016243927
4	12.327251672212437	18.07981770541347	38.99852975253039	30.594400869843714
5	12.728139498930746	38.2128093177065	35.69016418974954	13.368886993613216
6	29.65667264861354	38.43691584420884	18.33605131159813	13.57036019557949
7	25.96275140959484	32.99245016922035	23.200485736239244	17.844312684945567
8	25.231087704134175	35.16165219949978	20.99567709099603	18.611583005370015
9	26.013196263529874	15.543884673782987	21.036523340294867	37.40639572239227
10-11	24.147475514902546	26.273075645474407	29.03968189200466	20.539766947618386
12-13	25.378482896073017	24.121370268137667	28.68036291140601	21.819783924383305
14-15	22.735624164529742	25.286430526229886	26.77909907714659	25.19884623209378
16-17	21.77415600738607	28.13610431526299	27.608140190444665	22.481599486906283
18-19	21.57990651345532	27.657178455739288	29.35939849537639	21.403516535429
20-21	22.562360850420163	26.665441594819413	29.009232003512576	21.76296555124785
22-23	22.199488057451585	26.64071035189441	28.64690101773581	22.51290057291819
24-25	22.176040307142543	26.8193414055662	28.7971993731175	22.20741891417376
26-27	22.271320886735673	26.692240883258727	28.94045876983708	22.095979460168515
28-29	21.9156438447644	27.063976726958415	29.018731764058163	22.00164766421902
30-31	22.46655635280952	26.649575058896076	28.785221448818348	22.098647139476054
32-33	22.18391192686913	26.897060038433217	28.71770262746718	22.201325407230474
34-35	22.381824626304372	26.910467213101803	28.888917943208924	21.8187902173849
36-37	22.444276727824803	26.643365454753653	28.929795077780618	21.982562739640922
38-39	22.34512266697126	26.77211047821836	28.84411944934739	22.038647405462992
40-41	22.399109440130022	26.68935402800758	28.820841491722266	22.090695040140133
42-43	22.401495023962106	26.637937425540464	28.513886757710605	22.44668079278683
44-45	22.51151812534186	26.790655961674208	28.387338890998524	22.310487021985406
46-47	22.49853307885628	26.886020945507543	28.277311600487113	22.338134375149064
48-49	22.747996558118672	26.874662273656003	28.03884745537702	22.338493712848305
50-51	22.26524807777297	27.12598683132964	27.90888595025744	22.69987914063995
52-53	22.232415199141396	27.413539689632234	27.425421651607085	22.92862345961929
54-55	22.348318206700164	27.194549527092164	27.524471525675526	22.932660740532146
56-57	22.718650258004683	26.818751620835485	27.556365564223157	22.906232556936672
58-59	22.578850598691712	26.806064229314174	27.93863530271794	22.676449869276173
60-61	22.493539968147033	27.045037395269176	27.941748005238427	22.51967463134536
62-63	22.239969310962135	26.925765861031532	27.88025815572675	22.954006672279583
64-65	21.871660443123908	27.209850965427368	27.849925216914052	23.06856337453467
66-67	22.421765435169196	26.78395296134532	27.60262371188307	23.191657891602418
68-69	22.32086232406923	26.670025870756565	27.72101996815509	23.288091837019117
70-71	22.391165977720977	26.568332061765165	27.724424597444507	23.31607736306935
72-73	22.56070244344271	26.504590417093038	27.959043864496415	22.975663274967836
74-75	22.4007656362541	26.91985575210673	27.58908344771373	23.09029516392544
76-77	22.287638457926548	26.67238611722393	27.570922240568652	23.46905318428087
78-79	22.229287942101582	26.73699851667775	27.684750056465095	23.348963484755576
80-81	22.23340184785179	26.709311758108594	27.619142992855544	23.438143401184067
82-83	22.28590738619392	26.494793830037384	27.921984532709054	23.297314251059646
84-85	22.37112291167394	26.661436871778072	27.454943305575192	23.5124969109728
86-87	22.07707700804407	26.77304933969871	27.54722738973092	23.6026462625263
88-89	22.218524987083622	26.398574974468964	27.502228398329002	23.88067164011841
90-91	22.322335007147835	26.31574032168847	27.534909448790618	23.82701522237308
92-93	22.300871605404772	26.53371445566148	27.447474154633273	23.71793978430047
94-95	21.880249977407093	26.45449113230191	27.62489711067967	24.040361779611327
96-97	21.960405291746323	26.28294446562862	27.726166294211506	24.030483948413547
98-99	22.0784753202158	26.399531619289814	27.351077203795892	24.170915856698493
100	22.0822399967793	26.143053712359865	27.33327486229351	24.44143142856732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	140.0
2	190.5
3	312.0
4	216.5
5	178.0
6	354.0
7	850.5
8	1540.0
9	1909.0
10	1559.5
11	1113.5
12	1154.0
13	1360.5
14	1587.5
15	1859.0
16	2187.0
17	2480.5
18	2820.5
19	3340.0
20	3988.0
21	4810.0
22	6016.5
23	7584.0
24	9511.0
25	12277.0
26	16377.5
27	21114.0
28	26215.0
29	32999.0
30	40388.5
31	46878.0
32	54830.0
33	63351.5
34	70052.0
35	76702.0
36	84535.5
37	93211.5
38	98893.5
39	101363.5
40	104143.5
41	106206.5
42	107971.5
43	112694.5
44	118863.0
45	126120.0
46	135285.5
47	147509.5
48	159794.0
49	159745.0
50	157671.5
51	149844.5
52	133775.5
53	125073.0
54	116304.5
55	102968.5
56	91062.5
57	80803.5
58	70203.5
59	61270.0
60	51936.5
61	42123.0
62	33093.5
63	25470.5
64	18459.5
65	12709.0
66	8968.5
67	6180.5
68	4295.0
69	3111.0
70	2376.0
71	1857.0
72	1478.5
73	1340.0
74	1045.5
75	736.5
76	538.0
77	314.5
78	187.0
79	91.5
80	32.0
81	20.0
82	16.0
83	9.0
84	5.0
85	5.0
86	3.0
87	1.5
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0026436189073923053
2	0.003103378717373576
3	2.2987990499063525E-4
4	0.05034369919294913
5	0.0022987990499063527
6	0.004683803064184194
7	0.0
8	4.0228983373361173E-4
9	5.746997624765881E-5
10-11	5.746997624765881E-5
12-13	0.0
14-15	5.746997624765881E-5
16-17	3.448198574859529E-4
18-19	1.4367494061914705E-4
20-21	2.873498812382941E-4
22-23	0.0
24-25	0.0
26-27	3.017173753002088E-4
28-29	0.0
30-31	2.1551241092872055E-4
32-33	4.4539231591935585E-4
34-35	1.8677742280489115E-4
36-37	4.3102482185744114E-5
38-39	7.327421971576499E-4
40-41	5.746997624765882E-4
42-43	0.0
44-45	5.603322684146735E-4
46-47	0.0
48-49	1.2930744655723233E-4
50-51	0.0
52-53	3.735548456097823E-4
54-55	3.735548456097823E-4
56-57	1.5804243468106174E-4
58-59	9.051521259006264E-4
60-61	7.327421971576499E-4
62-63	2.2987990499063525E-4
64-65	3.735548456097823E-4
66-67	0.0013361769477580675
68-69	0.0024999439667731586
70-71	0.0035344035392310177
72-73	0.0031895836817450647
74-75	0.002772926353949538
76-77	0.004022898337336117
78-79	0.0014942193824391292
80-81	0.0010631945605816882
82-83	7.040072090338205E-4
84-85	5.172297862289293E-4
86-87	0.0
88-89	0.0
90-91	8.620496437148823E-5
92-93	1.5804243468106174E-4
94-95	1.0057245843340293E-4
96-97	4.884947981050999E-4
98-99	8.620496437148823E-5
100	0.07393512444261306
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3480078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.8790104591345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.02750168001975	45.18869540262405
2	15.202745537539549	18.814617002499403
3	5.546043214067035	10.29550998150198
4	2.516122215527177	6.227806115642675
5	1.3285394117874383	4.110435207868365
6	0.7582354921994199	2.8151317171376937
7	0.47469111496344546	2.056139152737688
8	0.29614699619055285	1.466022645977319
9	0.20688044414115775	1.1521401450120983
>10	0.6275966746890355	5.63957880367948
>50	0.009436513162871177	0.3983408085360142
>100	0.005119777612827125	0.6179591020028185
>500	5.37673199896411E-4	0.2455376594326352
>1k	2.6883659994789876E-4	0.31474068539711164
>5k	1.3441829997394938E-4	0.65734556995068
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8114	0.23315569363675182	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7692	0.22102952864849584	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4000	0.11493995249531763	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.746997624765882E-5	0.0
4	0.0	0.0	0.0	5.746997624765882E-5	0.0
5	0.0	0.0	0.0	5.746997624765882E-5	0.0
6	0.0	0.0	0.0	5.746997624765882E-5	0.0
7	0.0	0.0	0.0	5.746997624765882E-5	0.0
8	0.0	0.0	0.0	5.746997624765882E-5	0.0
9	0.0	0.0	0.0	1.4367494061914705E-4	0.0
10-11	0.0	0.0	0.0	1.4367494061914705E-4	0.0
12-13	0.0	0.0	0.0	1.5804243468106176E-4	0.0
14-15	0.0	0.0	0.0	2.0114491686680586E-4	1.4367494061914705E-5
16-17	0.0	0.0	0.0	2.7298238717637935E-4	5.746997624765882E-5
18-19	0.0	0.0	0.0	3.160848693621235E-4	5.746997624765882E-5
20-21	0.0	0.0	0.0	5.315972802908441E-4	5.746997624765882E-5
22-23	2.873498812382941E-5	0.0	0.0	7.040072090338205E-4	5.746997624765882E-5
24-25	5.746997624765882E-5	0.0	0.0	0.0012356044893246645	5.746997624765882E-5
26-27	5.746997624765882E-5	0.0	0.0	0.0019683466864823147	8.620496437148823E-5
28-29	5.746997624765882E-5	0.0	0.0	0.004655068076060365	8.620496437148823E-5
30-31	5.746997624765882E-5	0.0	0.0	0.013146257066651953	8.620496437148823E-5
32-33	5.746997624765882E-5	0.0	0.0	0.032844091425537014	8.620496437148823E-5
34-35	5.746997624765882E-5	0.0	0.0	0.06415086098644915	8.620496437148823E-5
36-37	5.746997624765882E-5	0.0	0.0	0.11287103335040191	8.620496437148823E-5
38-39	5.746997624765882E-5	0.0	0.0	0.1883722146457637	1.1493995249531764E-4
40-41	5.746997624765882E-5	0.0	0.0	0.27479269142818064	1.1493995249531764E-4
42-43	5.746997624765882E-5	0.0	0.0	0.3612275357046595	1.1493995249531764E-4
44-45	8.620496437148823E-5	0.0	0.0	0.4680929565371811	1.2930744655723233E-4
46-47	1.1493995249531764E-4	0.0	0.0	0.5987509475362334	1.4367494061914705E-4
48-49	1.1493995249531764E-4	0.0	0.0	0.7382593148774251	1.7240992874297645E-4
50-51	1.1493995249531764E-4	0.0	0.0	0.8840319096296118	1.7240992874297645E-4
52-53	1.1493995249531764E-4	0.0	0.0	1.0349480672559639	2.2987990499063527E-4
54-55	1.1493995249531764E-4	0.0	0.0	1.203363832649728	2.2987990499063527E-4
56-57	1.1493995249531764E-4	0.0	0.0	1.4036754348609428	2.4424739905254996E-4
58-59	1.1493995249531764E-4	0.0	0.0	1.6280813246139885	2.5861489311446465E-4
60-61	1.1493995249531764E-4	0.0	0.0	1.8644553369206092	2.5861489311446465E-4
62-63	1.1493995249531764E-4	0.0	0.0	2.1046223676595757	2.873498812382941E-4
64-65	1.1493995249531764E-4	0.0	0.0	2.361383854040053	2.873498812382941E-4
66-67	1.1493995249531764E-4	0.0	0.0	2.653015248508798	3.160848693621235E-4
68-69	1.1493995249531764E-4	0.0	0.0	2.981441795270106	3.160848693621235E-4
70-71	1.1493995249531764E-4	0.0	0.0	3.342267041141032	3.160848693621235E-4
72-73	1.1493995249531764E-4	0.0	0.0	3.7019141524988806	3.160848693621235E-4
74-75	1.1493995249531764E-4	0.0	0.0	4.060383129343652	3.160848693621235E-4
76-77	1.1493995249531764E-4	0.0	0.0	4.458262142400256	3.160848693621235E-4
78-79	1.1493995249531764E-4	0.0	0.0	4.902418853830287	3.160848693621235E-4
80-81	1.1493995249531764E-4	0.0	0.0	5.383744272398492	3.160848693621235E-4
82-83	1.1493995249531764E-4	0.0	0.0	5.879020527700816	3.160848693621235E-4
84-85	1.1493995249531764E-4	0.0	0.0	6.370963524380775	3.160848693621235E-4
86-87	1.1493995249531764E-4	0.0	0.0	6.870291413008559	3.448198574859529E-4
88	1.1493995249531764E-4	0.0	0.0	7.272480674283737	3.735548456097823E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGC	475	0.0	21.780615	3
GAGTACT	9610	0.0	21.640575	12-13
TAACGCC	565	0.0	20.808111	4
GTACTTT	10175	0.0	20.207964	14-15
TCTAACG	515	0.0	20.088919	2
AGTACTT	10170	0.0	18.577362	12-13
GTCGACG	375	0.0	17.54565	36-37
TATACTG	1600	0.0	17.33224	5
CATGGGG	6535	0.0	17.198612	4
CTGTGCG	1450	0.0	16.854197	9
ACTTTTT	12455	0.0	16.810467	16-17
TACCTGG	2950	0.0	16.738184	2
GTACTAG	1145	0.0	16.42865	1
TAGTACT	1805	0.0	16.413622	4
ACATGGG	15765	0.0	16.406267	3
TCGACGA	405	0.0	16.245974	36-37
TACATGG	17320	0.0	16.209421	2
GTCTTAT	1175	0.0	16.009193	1
CGGGCGT	920	0.0	15.837404	6
CTAGTAC	1575	0.0	15.824733	3
>>END_MODULE