FastQCFastQC Report
Mon 16 Jan 2017
ERR1146620_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146620_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290847
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2352267.14788624326807No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC602451.8306837115186454No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT556051.6896865761307043No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC426121.294864209730808No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA408871.242446093665248No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA371411.1286152166904144No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG362741.102269415746159No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC320300.9733056565680508No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG228080.6930738499845177No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG199460.6061053582861798No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA104520.31760820238680193No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG103050.3131412672786064No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51230.15567420788629796No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38000.11547179191253802No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33780.10264834554751406No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC650.006162947528.9162169
GTACGCA23050.027.5310553
GAGTACG25250.026.0754341
AGTACGC25200.025.368712
GTACTAG8400.025.1940561
TACGCAG25300.025.0822584
TAGGCAT11450.023.399665
GTGATCG5250.023.2706728
TATACCG4400.022.4340065
TGATCGC5600.021.8162549
GCTTATG13150.021.8156911
TACCGAC3251.8189894E-1221.6874927
CGGGCGT5400.020.8836156
TACGGGC5650.020.799124
CTAGGCA14750.020.3958284
ATACCGA4150.020.3803986
GCATCGT9300.020.2102588
GTATAAC4950.019.9516561
TCTAACG3553.6379788E-1219.8619962
CGCAGTG32350.019.75396
Produced by FastQC (version 0.11.5)