##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146620_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3290847
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.602216086010685	31.0	31.0	34.0	30.0	34.0
2	31.72239213795111	33.0	31.0	34.0	30.0	34.0
3	31.911632780253836	33.0	31.0	34.0	30.0	34.0
4	35.29538170568246	37.0	35.0	37.0	33.0	37.0
5	35.46689347757584	37.0	35.0	37.0	33.0	37.0
6	35.61631610342261	37.0	35.0	37.0	35.0	37.0
7	35.588317536488326	37.0	35.0	37.0	35.0	37.0
8	35.60938566879591	37.0	35.0	37.0	35.0	37.0
9	37.2873163048905	39.0	38.0	39.0	35.0	39.0
10-11	37.26872929674336	39.0	38.0	39.0	35.0	39.0
12-13	37.27232487563232	39.0	38.0	39.0	35.0	39.0
14-15	38.45680002747013	40.0	38.0	41.0	34.5	41.0
16-17	38.62987932893872	40.0	38.5	41.0	35.0	41.0
18-19	38.72069424680029	40.5	39.0	41.0	35.0	41.0
20-21	38.73058577320671	40.5	39.0	41.0	35.0	41.0
22-23	38.684355425821984	40.0	39.0	41.0	34.5	41.0
24-25	38.60255125808037	40.0	38.5	41.0	34.0	41.0
26-27	38.45313501356945	40.0	38.0	41.0	34.0	41.0
28-29	38.49891957906277	40.0	38.0	41.0	34.0	41.0
30-31	38.573056571757974	40.0	38.0	41.0	34.0	41.0
32-33	38.43993522032474	40.0	38.0	41.0	34.0	41.0
34-35	38.214977633417774	40.0	38.0	41.0	33.0	41.0
36-37	38.04744887866254	40.0	38.0	41.0	33.0	41.0
38-39	37.93537605971958	40.0	38.0	41.0	33.0	41.0
40-41	38.10074761907801	40.0	38.0	41.0	33.0	41.0
42-43	37.928444409600324	40.0	38.0	41.0	33.0	41.0
44-45	37.72421674419989	40.0	37.5	41.0	32.0	41.0
46-47	37.47666694926868	40.0	37.0	41.0	31.0	41.0
48-49	37.24635086347071	40.0	37.0	41.0	31.0	41.0
50-51	36.93748098893689	39.0	36.0	41.0	30.0	41.0
52-53	37.01562302957262	39.0	36.0	41.0	30.5	41.0
54-55	36.850510825936304	39.0	35.5	41.0	30.0	41.0
56-57	36.25756013573405	38.5	34.5	40.5	28.5	41.0
58-59	36.00922832328577	38.0	35.0	40.0	28.0	41.0
60-61	35.4067202151908	38.0	34.0	40.0	27.5	41.0
62-63	35.469999212968574	38.0	34.0	40.0	28.0	41.0
64-65	35.21463349709057	37.0	34.0	39.5	28.0	41.0
66-67	34.59831435493659	36.0	33.0	39.0	26.5	41.0
68-69	34.0639207778423	36.0	32.5	39.0	26.0	40.0
70-71	33.40150104213292	35.0	31.5	37.5	25.5	40.0
72-73	33.03569035570477	35.0	31.5	37.0	25.0	39.0
74-75	32.90597952442031	35.0	31.5	37.0	26.0	39.0
76-77	32.69516571265696	35.0	32.0	36.0	26.0	38.5
78-79	32.21993091748112	34.5	31.0	36.0	25.0	37.0
80-81	31.664449608261947	34.0	30.5	35.0	22.5	37.0
82-83	31.356349444383163	34.0	30.0	35.0	21.5	36.5
84-85	31.283887248480404	34.0	30.0	35.0	23.0	36.0
86-87	31.573032717716746	34.0	31.0	35.0	24.0	36.0
88-89	31.238160266946473	34.0	30.5	35.0	22.5	36.0
90-91	30.838224019530536	34.0	30.0	35.0	20.0	35.0
92-93	30.524397822202005	34.0	29.0	35.0	20.0	35.0
94-95	30.446808222928624	34.0	29.0	35.0	19.5	35.0
96-97	30.403957096759587	34.0	29.0	35.0	19.0	35.0
98-99	30.581054512713596	34.0	29.0	35.0	19.0	35.0
100	29.104868138810463	33.0	27.0	35.0	16.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20015032470337601
1101	2	0.28089290156387037
1101	3	0.28266195441645436
1101	4	0.2771735114679714
1101	5	0.18472006391461804
1101	6	0.21653966795801693
1101	7	0.2861053802025353
1101	8	0.3207105606694114
1101	9	0.4140976887828245
1101	10-11	0.35292716870755925
1101	12-13	0.3581863466399753
1101	14-15	0.5056282894940551
1101	16-17	0.49806308435307045
1101	18-19	0.45913623876955967
1101	20-21	0.47109001125305383
1101	22-23	0.47656051824376533
1101	24-25	0.5385418843048129
1101	26-27	0.5601508378794335
1101	28-29	0.48827161613061776
1101	30-31	0.5042423781301721
1101	32-33	0.48203220848580486
1101	34-35	0.5085941481340157
1101	36-37	0.5824646457968399
1101	38-39	0.5731915227854145
1101	40-41	0.5510053792073322
1101	42-43	0.5722114827164262
1101	44-45	0.5692343904464678
1101	46-47	0.6429085527676648
1101	48-49	0.687181906264918
1101	50-51	0.6845062937471198
1101	52-53	0.6328456035246575
1101	54-55	0.6686115025494246
1101	56-57	0.6806399261769371
1101	58-59	0.8197456294308054
1101	60-61	0.8427211707717746
1101	62-63	0.8089653041504477
1101	64-65	0.8128088193100709
1101	66-67	0.8176011599075679
1101	68-69	0.8040491871992614
1101	70-71	0.7654818333517923
1101	72-73	0.7874064373834884
1101	74-75	0.8509177465687898
1101	76-77	0.7297776802600424
1101	78-79	0.7908971026038927
1101	80-81	0.7751809597653754
1101	82-83	0.773572719127305
1101	84-85	0.7124746925572971
1101	86-87	0.7076015764452102
1101	88-89	0.7446719380325639
1101	90-91	0.708289135455761
1101	92-93	0.6921628274184393
1101	94-95	0.7786751437238522
1101	96-97	0.6924685658723497
1101	98-99	0.7254959768120486
1101	100	0.7430872625206355
1102	1	0.20748919906341357
1102	2	0.3319731227109166
1102	3	0.320006900095855
1102	4	0.28892024810450323
1102	5	0.30970382448373357
1102	6	0.2623378529876277
1102	7	0.26829365582589304
1102	8	0.31266184143873943
1102	9	0.39977316234104165
1102	10-11	0.3962209345014287
1102	12-13	0.38550118664230837
1102	14-15	0.5366808660878917
1102	16-17	0.46017572995686606
1102	18-19	0.45747949437561886
1102	20-21	0.5395893670694178
1102	22-23	0.5497414029914083
1102	24-25	0.5740163348140683
1102	26-27	0.5322274882277185
1102	28-29	0.5319252289525664
1102	30-31	0.5601303502110113
1102	32-33	0.49678417785390394
1102	34-35	0.5437866070816781
1102	36-37	0.602187903616624
1102	38-39	0.5649923689711542
1102	40-41	0.4867278943516382
1102	42-43	0.497481930798088
1102	44-45	0.5288804013387249
1102	46-47	0.6020077579852909
1102	48-49	0.615982935598602
1102	50-51	0.6675217543888436
1102	52-53	0.6195212677349673
1102	54-55	0.5668882351149591
1102	56-57	0.7000979008748516
1102	58-59	0.6308559269418694
1102	60-61	0.7724715912381939
1102	62-63	0.6423104017887056
1102	64-65	0.7186259827821573
1102	66-67	0.7282075191514465
1102	68-69	0.6763720261472557
1102	70-71	0.6873653634499632
1102	72-73	0.7585859617710042
1102	74-75	0.7514242057584539
1102	76-77	0.6516496610156679
1102	78-79	0.5882054667667376
1102	80-81	0.6327370262539382
1102	82-83	0.6607007892591277
1102	84-85	0.6419350603727842
1102	86-87	0.5216890540969423
1102	88-89	0.5850839142200464
1102	90-91	0.5976989042531642
1102	92-93	0.7283298574106176
1102	94-95	0.6918989826455828
1102	96-97	0.7465171439313636
1102	98-99	0.5562196668234733
1102	100	0.6315783602639549
1103	1	0.12350502451365841
1103	2	0.21118495565673356
1103	3	0.22084347159924533
1103	4	0.2171559629968982
1103	5	0.1850768554816895
1103	6	0.21418124322782006
1103	7	0.19861493803507102
1103	8	0.21001903902373442
1103	9	0.206087350657441
1103	10-11	0.26568413924579204
1103	12-13	0.2580867150302666
1103	14-15	0.3647761165063699
1103	16-17	0.3035878676091741
1103	18-19	0.3794749304137994
1103	20-21	0.37640788046623896
1103	22-23	0.41588681054076915
1103	24-25	0.45813093197154586
1103	26-27	0.4078467550741607
1103	28-29	0.41352161803904863
1103	30-31	0.46140431797795145
1103	32-33	0.447065438581447
1103	34-35	0.43924829183254843
1103	36-37	0.4748043428151192
1103	38-39	0.49642621917305974
1103	40-41	0.4183694386579404
1103	42-43	0.41065346400967684
1103	44-45	0.4446472814061124
1103	46-47	0.46263096858667296
1103	48-49	0.4547015514490056
1103	50-51	0.5157057138568604
1103	52-53	0.4175037484386621
1103	54-55	0.4351282233301461
1103	56-57	0.4312564070875311
1103	58-59	0.4994568361952503
1103	60-61	0.5346249588465852
1103	62-63	0.5877386099658821
1103	64-65	0.4893232864304977
1103	66-67	0.5214810502962806
1103	68-69	0.42980716276971265
1103	70-71	0.40985817046564677
1103	72-73	0.4448654547762061
1103	74-75	0.46130075716042285
1103	76-77	0.40652383687485383
1103	78-79	0.39502976640186915
1103	80-81	0.3682008167000994
1103	82-83	0.48391268457953274
1103	84-85	0.505283841369863
1103	86-87	0.4672608122994184
1103	88-89	0.4105474879034574
1103	90-91	0.39534302493958506
1103	92-93	0.3685755778946067
1103	94-95	0.40275765731389157
1103	96-97	0.39769102481625396
1103	98-99	0.29623929048795006
1103	100	0.43433432168195196
1104	1	-0.019970643230021068
1104	2	0.019704202754248
1104	3	0.08827430198060426
1104	4	0.08280294166078761
1104	5	0.034353199455125605
1104	6	0.062300886537784095
1104	7	0.09390403135162728
1104	8	0.18203831098490753
1104	9	0.20395772420226166
1104	10-11	0.19773083227102717
1104	12-13	0.19950198848263767
1104	14-15	0.223213715053717
1104	16-17	0.2592737231522406
1104	18-19	0.2573003146345485
1104	20-21	0.243835544977955
1104	22-23	0.2669207526785513
1104	24-25	0.3194253803952094
1104	26-27	0.33126236086563665
1104	28-29	0.2948689164094418
1104	30-31	0.2620209367241628
1104	32-33	0.3057688157458571
1104	34-35	0.2612777717928978
1104	36-37	0.33739509903172404
1104	38-39	0.28403659605979215
1104	40-41	0.31337097835161387
1104	42-43	0.37069439793195613
1104	44-45	0.3158854175026775
1104	46-47	0.37652433344898384
1104	48-49	0.43453411246458273
1104	50-51	0.4470820146818042
1104	52-53	0.37561991083634183
1104	54-55	0.4032689961173759
1104	56-57	0.46814913537839686
1104	58-59	0.519028628389421
1104	60-61	0.487977491248877
1104	62-63	0.41701986180942185
1104	64-65	0.539381399442469
1104	66-67	0.4320508697675791
1104	68-69	0.38823581966573073
1104	70-71	0.4228629452147885
1104	72-73	0.47416287571980575
1104	74-75	0.5073177534018427
1104	76-77	0.31925733520404265
1104	78-79	0.32471363964724276
1104	80-81	0.26378433589315264
1104	82-83	0.4300030981100562
1104	84-85	0.41278608759297697
1104	86-87	0.3585075533840971
1104	88-89	0.3716469805303788
1104	90-91	0.3552319971191622
1104	92-93	0.39914081380440436
1104	94-95	0.312472305900819
1104	96-97	0.34927476268595115
1104	98-99	0.3786673395369995
1104	100	0.34539346105341906
1105	1	0.16653763286177892
1105	2	0.12327840041557891
1105	3	0.14456105908506345
1105	4	0.1676037832453261
1105	5	0.15182675156980707
1105	6	0.21880172505922957
1105	7	0.2107291651704628
1105	8	0.23610222048585428
1105	9	0.3432632255155639
1105	10-11	0.3090516136275099
1105	12-13	0.2971243111487567
1105	14-15	0.4082564451876465
1105	16-17	0.3621507562795827
1105	18-19	0.35459962467307093
1105	20-21	0.37926345893570357
1105	22-23	0.3570336180479998
1105	24-25	0.433019526443438
1105	26-27	0.37853161431353044
1105	28-29	0.38117914445665235
1105	30-31	0.3693745592133837
1105	32-33	0.429878413178983
1105	34-35	0.39023715327804354
1105	36-37	0.40065871830469746
1105	38-39	0.39463503809460576
1105	40-41	0.43021384460170253
1105	42-43	0.4336995209533896
1105	44-45	0.4698763871244651
1105	46-47	0.5187251507202291
1105	48-49	0.523107343337486
1105	50-51	0.557993753599149
1105	52-53	0.4575625285930869
1105	54-55	0.49799181500441136
1105	56-57	0.5509821796324132
1105	58-59	0.5329115665838771
1105	60-61	0.5573261582295075
1105	62-63	0.4455760638050279
1105	64-65	0.5720479359277206
1105	66-67	0.49603457890402325
1105	68-69	0.4803113851891041
1105	70-71	0.4252815914046906
1105	72-73	0.4335102839610556
1105	74-75	0.45842769351838797
1105	76-77	0.46841969918361315
1105	78-79	0.5472037228867634
1105	80-81	0.4845623421265408
1105	82-83	0.34083536414725657
1105	84-85	0.41455203194425394
1105	86-87	0.3309069403828069
1105	88-89	0.3621831508612665
1105	90-91	0.38385891346611345
1105	92-93	0.39165655522583265
1105	94-95	0.4569934153910431
1105	96-97	0.4801970596986749
1105	98-99	0.47183973131413026
1105	100	0.3919478653968831
1106	1	0.13536552622787923
1106	2	0.1948189509478624
1106	3	0.12864124602914018
1106	4	0.1936259612431641
1106	5	0.20491071270957661
1106	6	0.1861083986906209
1106	7	0.16512545019948277
1106	8	0.22372467032877807
1106	9	0.2789445487647555
1106	10-11	0.2564819832117635
1106	12-13	0.27253056137866594
1106	14-15	0.3680787342777947
1106	16-17	0.2751777780124698
1106	18-19	0.2575319834350154
1106	20-21	0.31577070936425145
1106	22-23	0.34241417358312276
1106	24-25	0.3203802472191697
1106	26-27	0.3229026920560756
1106	28-29	0.29611669181802114
1106	30-31	0.289929671298907
1106	32-33	0.2895386619963318
1106	34-35	0.28238735044849506
1106	36-37	0.3076232772829499
1106	38-39	0.22583013568662835
1106	40-41	0.28134695397244514
1106	42-43	0.3141476423162217
1106	44-45	0.3101354400441352
1106	46-47	0.3807227293125166
1106	48-49	0.423502327997916
1106	50-51	0.41064796774981005
1106	52-53	0.4409408705587623
1106	54-55	0.3379260516678073
1106	56-57	0.42093598619936756
1106	58-59	0.35400967911818526
1106	60-61	0.4801294411544035
1106	62-63	0.3964039682067835
1106	64-65	0.43188089282243425
1106	66-67	0.4248695396906399
1106	68-69	0.3604799594922028
1106	70-71	0.4138442794707231
1106	72-73	0.373964070295969
1106	74-75	0.35573467063043296
1106	76-77	0.30720716984714613
1106	78-79	0.2854134997457294
1106	80-81	0.29657254275483425
1106	82-83	0.3224376086165499
1106	84-85	0.27098556876289237
1106	86-87	0.20305537184767886
1106	88-89	0.2247904956447151
1106	90-91	0.18260358416202394
1106	92-93	0.3727901520048924
1106	94-95	0.2840520947536227
1106	96-97	0.3079579978769047
1106	98-99	0.2621443293218242
1106	100	0.4055540983375927
1107	1	0.09919269915817708
1107	2	0.12151392888021917
1107	3	0.16296878551081306
1107	4	0.14465715490083397
1107	5	0.06830997602789068
1107	6	0.10530844822591234
1107	7	0.11785913020568017
1107	8	0.2007355848750123
1107	9	0.25492238002969003
1107	10-11	0.21097969031262664
1107	12-13	0.2445412068371482
1107	14-15	0.27462460421266144
1107	16-17	0.26816493734104796
1107	18-19	0.29192722886596556
1107	20-21	0.2865069605940036
1107	22-23	0.31205328573867064
1107	24-25	0.34876604435390135
1107	26-27	0.33088849971393586
1107	28-29	0.31050994835888446
1107	30-31	0.3314988316796956
1107	32-33	0.3330986265648903
1107	34-35	0.21549970390862683
1107	36-37	0.24265463368416818
1107	38-39	0.31423919322025995
1107	40-41	0.29568438982929734
1107	42-43	0.22866883683790462
1107	44-45	0.2262432061924997
1107	46-47	0.3580118124827081
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2216	82-83	-1.9203998130225948
2216	84-85	-1.8169944080523486
2216	86-87	-1.6832112822326586
2216	88-89	-1.7107811926431218
2216	90-91	-1.916664767856652
2216	92-93	-1.954153579033207
2216	94-95	-2.021044600535113
2216	96-97	-1.848772124225313
2216	98-99	-1.8021903082488429
2216	100	-1.7139459283442484
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	982.0
1	235.0
2	195.0
3	187.0
4	139.0
5	187.0
6	267.0
7	635.0
8	1563.0
9	1893.0
10	1939.0
11	2389.0
12	3014.0
13	3816.0
14	5045.0
15	5583.0
16	6265.0
17	6915.0
18	7814.0
19	9111.0
20	10753.0
21	12680.0
22	14983.0
23	17998.0
24	21706.0
25	27038.0
26	31491.0
27	35462.0
28	41120.0
29	52053.0
30	65062.0
31	81657.0
32	108585.0
33	150772.0
34	222625.0
35	332428.0
36	502374.0
37	727410.0
38	620675.0
39	155531.0
40	270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.43315715426442	17.3739229628503	13.058352028594244	19.13456785429104
2	13.280310692081695	26.909943204796605	32.030816217435806	27.778929885685894
3	30.791025143891183	22.084411091596145	29.94293013389293	17.18163363061974
4	10.902458014245656	14.570410155655988	45.381688675859635	29.14544315423872
5	10.70230706157436	43.27309798807296	34.19412212681783	11.83047282353485
6	26.68707952467868	38.51989645346109	15.440764743078725	19.352259278781506
7	25.026338712189045	33.3781740277183	22.968908153930006	18.626579106162648
8	23.278498456904178	23.76337149480621	20.15395372871989	32.80417631956972
9	19.476986760605868	24.213591666139557	27.057738063379766	29.25168350987481
10-11	27.050202136353008	21.49409070878345	27.092308174228062	24.36339898063548
12-13	34.138565640421206	21.471756659283344	23.861666684912112	20.528011015383342
14-15	24.299811081131715	24.898059232695008	31.917237599842345	18.884892086330936
16-17	19.759263908426796	27.72561614550062	30.05577497317239	22.459344972900197
18-19	18.43540087946564	36.147747064975775	24.552337146946307	20.86451490861227
20-21	23.83451335708839	25.843576973457637	21.68050182116197	28.641407848292
22-23	20.565176157963215	26.865953964749707	25.003310996965133	27.565558880321944
24-25	28.258023764554434	26.337180048819782	22.707993831914656	22.69680235471113
26-27	25.13119892592877	20.979770695523463	32.85140519504413	21.037625183503643
28-29	19.47007712613895	34.89138170139776	24.928196051239006	20.710345121224286
30-31	22.597392327835546	25.231874184532195	24.20709917000964	27.96363431762262
32-33	24.261778738461228	26.666326027536414	26.28023286334258	22.79166237065978
34-35	28.491928254189848	24.032078640877835	25.56219361696789	21.91379948796443
36-37	24.84165566530473	21.98113934924961	32.36029132103178	20.816913664413878
38-39	22.413877269834366	28.267280243991273	28.854294901868943	20.464547584305418
40-41	21.636289038810247	30.08397948141484	25.890358296638695	22.389373183136218
42-43	20.234323316903733	29.813596774708657	20.750084023645243	29.20199588474237
44-45	23.914803551316993	23.612027347488844	23.59566435425333	28.877504746940836
46-47	28.534960772840133	27.952544808012586	25.122809173157894	18.38968524598939
48-49	25.429263526987057	26.273829555035643	26.4027485275528	21.8941583904245
50-51	16.646945134398152	28.29200682571331	33.5998033980446	21.461244641843948
52-53	22.672929613930243	31.416606036567856	22.707300188338582	23.20316416116332
54-55	19.50777506213602	24.922854619355295	26.796409188953106	28.772961129555576
56-57	26.12442789130182	22.027555507565637	25.93657777294499	25.911438828187556
58-59	31.838063000070417	23.07390240204137	23.652105434375446	21.43592916351277
60-61	23.835852565455728	25.743435568714602	32.158139481123555	18.26257238470612
62-63	20.30121923083533	26.799520197906958	29.689647982399347	23.209612588858366
64-65	18.687623915671338	34.549833643496406	24.550694475285002	22.21184796554726
66-67	23.386463139124977	25.406595258439356	22.23689065464369	28.970050947791975
68-69	20.30843379357597	26.558126766593336	25.526713818913777	27.606725620916922
70-71	27.488549903924742	25.98536833309658	23.008875668549642	23.517206094429035
72-73	24.502273147927763	21.042096847219184	32.518676496062575	21.936953508790474
74-75	19.756254994307213	33.69964590038671	25.114015451896023	21.43008365341006
76-77	22.450640759877853	24.55557748995386	24.848437194399164	28.14534455576912
78-79	23.807403128382756	25.754349205219334	27.14551386092186	23.292733805476047
80-81	27.467536546337808	23.921001476956153	26.000405474730147	22.61105650197589
82-83	23.999750561237214	21.91914229568836	32.010050556978506	22.07105658609592
84-85	21.93141418788665	27.762402138411176	28.587698052083848	21.718485621618335
86-87	21.55821738118739	29.282508550979657	26.17378449858333	22.98548956924963
88-89	20.456687421591113	28.622922093219373	21.911052974408648	29.009337510780863
90-91	23.6365776625238	23.04774717626148	24.6074628184505	28.708212342764224
92-93	27.581483079413395	27.111824587427304	25.684310960974226	19.622381372185078
94-95	24.421598091616637	25.69974411530597	26.75865599248618	23.120001800591215
96-97	17.30867171782993	27.09473660839271	32.91488354459601	22.68170812918135
98-99	22.93184446657594	30.007981938430785	23.449225563374583	23.610948031618697
100	22.179389193221706	19.955720362023204	24.369945774502227	33.49494467025286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1217.0
1	766.0
2	336.0
3	350.5
4	331.5
5	288.0
6	240.0
7	229.0
8	249.0
9	255.5
10	238.5
11	228.5
12	238.5
13	253.0
14	275.5
15	325.0
16	337.0
17	381.5
18	477.5
19	582.5
20	759.5
21	1085.0
22	1627.0
23	2269.0
24	3235.0
25	4437.0
26	6069.5
27	8443.5
28	10843.0
29	13929.5
30	18009.5
31	21695.0
32	26262.5
33	30988.0
34	34357.0
35	38059.0
36	41944.0
37	46048.5
38	48990.0
39	49706.0
40	49256.5
41	48898.0
42	49853.0
43	53595.5
44	60867.0
45	71240.0
46	101229.0
47	264863.5
48	599800.0
49	597353.0
50	285444.5
51	134106.5
52	85298.0
53	71109.0
54	64742.5
55	57001.5
56	50731.0
57	44241.5
58	37703.5
59	33435.0
60	28194.5
61	22012.0
62	17162.0
63	13236.0
64	9505.5
65	6414.5
66	4612.5
67	3370.5
68	2391.0
69	1749.5
70	1254.0
71	976.0
72	842.0
73	739.0
74	553.5
75	384.5
76	286.0
77	187.5
78	127.0
79	72.5
80	48.0
81	37.0
82	22.0
83	15.5
84	14.5
85	16.5
86	14.5
87	9.5
88	7.5
89	9.0
90	8.0
91	4.0
92	3.5
93	7.0
94	7.0
95	7.0
96	7.0
97	5.5
98	4.0
99	2.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10550475303166632
2	0.06651782960435414
3	0.08423363346883037
4	0.08590493572019604
5	0.08675578050270948
6	0.09268130666664236
7	0.08845747006773637
8	0.09043264545571399
9	0.07533015056610046
10-11	0.08265349315844828
12-13	0.07156212367211237
14-15	0.0809214162797602
16-17	0.08275984875626244
18-19	0.0824711692764811
20-21	0.07367404197156538
22-23	0.07786749125681017
24-25	0.07993382858577139
26-27	0.07367404197156538
28-29	0.08286620435407663
30-31	0.08681655513003188
32-33	0.08842708275407517
34-35	0.0908428741901401
36-37	0.09105558538576847
38-39	0.0919064301682819
40-41	0.08815359693112441
42-43	0.09365370070380057
44-45	0.08933870216391099
46-47	0.08566183721090649
48-49	0.09489958056390954
50-51	0.09072132493549533
52-53	0.09575042534642297
54-55	0.08809282230380204
56-57	0.09479322496609535
58-59	0.09868280111472823
60-61	0.09739134028412746
62-63	0.09850047723276105
64-65	0.10115936717811555
66-67	0.10222292315625733
68-69	0.0993513220152745
70-71	0.1008858813551648
72-73	0.09801428021418193
74-75	0.10191905001964541
76-77	0.09924496641746029
78-79	0.106158080275382
80-81	0.10156959591254167
82-83	0.10535281646336034
84-85	0.10208618024478197
86-87	0.09760405147975582
88-89	0.09704188617702372
90-91	0.09810544215516552
92-93	0.09214952867757145
94-95	0.09231665890270803
96-97	0.0898704801529819
98-99	0.1040765492895902
100	0.2453471705004821
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3290847.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.783902016332114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78871842279821	37.98062948310437
2	14.37428133725476	14.59964189972674
3	5.135056711212902	7.823346506116341
4	2.3613284608743257	4.796698927416723
5	1.1969233814364444	3.0392219861962655
6	0.7079194167629919	2.1570546177810455
7	0.4160879506956298	1.4791398802812308
8	0.26133104611912783	1.0617128191951486
9	0.18363225257281943	0.8393006089526773
>10	0.563183857425949	4.177962182391104
>50	0.007699814759417451	0.2601050279302442
>100	0.0024000330078283124	0.20565801424688215
>500	3.422178760798118E-4	0.1295442518347246
>1k	2.053307256462799E-4	0.2941944050236935
>5k	6.844357521542662E-5	0.178066864004771
>10k+	8.213229025851196E-4	20.977722525798058
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	235226	7.14788624326807	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	60245	1.8306837115186454	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	55605	1.6896865761307043	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	42612	1.294864209730808	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	40887	1.242446093665248	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	37141	1.1286152166904144	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	36274	1.102269415746159	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	32030	0.9733056565680508	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	22808	0.6930738499845177	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	19946	0.6061053582861798	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	10452	0.31760820238680193	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	10305	0.3131412672786064	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5123	0.15567420788629796	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	3800	0.11547179191253802	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	3378	0.10264834554751406	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.077462732238843E-5
2	0.0	0.0	0.0	0.0	6.077462732238843E-5
3	0.0	0.0	0.0	0.0	6.077462732238843E-5
4	0.0	0.0	0.0	0.0	6.077462732238843E-5
5	0.0	0.0	0.0	0.0	9.116194098358264E-5
6	0.0	0.0	0.0	0.0	1.8232388196716528E-4
7	0.0	0.0	0.0	3.0387313661194215E-5	1.8232388196716528E-4
8	0.0	0.0	0.0	6.077462732238843E-5	1.8232388196716528E-4
9	0.0	0.0	0.0	9.116194098358264E-5	1.8232388196716528E-4
10-11	0.0	0.0	0.0	2.73485822950748E-4	1.8232388196716528E-4
12-13	0.0	0.0	0.0	5.62165302732093E-4	4.4061604808731615E-4
14-15	0.0	0.0	0.0	8.508447825134381E-4	6.229399300544814E-4
16-17	0.0	0.0	0.0	0.0013978164284149338	7.596828415298554E-4
18-19	0.0	0.0	0.0	0.002081530985791804	7.596828415298554E-4
20-21	0.0	0.0	0.0	0.004056706373769428	8.812320961746322E-4
22-23	0.0	0.0	0.0	0.0063053675846978	8.812320961746322E-4
24-25	0.0	0.0	0.0	0.008751546334423934	8.812320961746322E-4
26-27	0.0	0.0	0.0	0.010574785154095587	8.812320961746322E-4
28-29	3.0387313661194215E-5	0.0	0.0	0.01756386729617026	8.812320961746322E-4
30-31	3.0387313661194215E-5	0.0	0.0	0.031617999864472585	8.964257530052293E-4
32-33	3.0387313661194215E-5	0.0	0.0	0.07578596027101837	9.572003803276178E-4
34-35	3.0387313661194215E-5	0.0	0.0	0.1308021916546105	0.001002781350819409
36-37	3.0387313661194215E-5	0.0	0.0	0.19137930143820117	0.001002781350819409
38-39	3.0387313661194215E-5	0.0	0.0	0.2838478969092152	0.0010331686644806032
40-41	3.0387313661194215E-5	0.0	0.0	0.38573655961519937	0.0010331686644806032
42-43	3.0387313661194215E-5	0.0	0.0	0.5009804466752784	0.0010331686644806032
44-45	3.0387313661194215E-5	0.0	0.0	0.6459279328391748	0.0010635559781417975
46-47	3.0387313661194215E-5	0.0	0.0	0.835651125682841	0.0010939432918029917
48-49	3.0387313661194215E-5	0.0	0.0	0.9865089443538395	0.001139524262294783
50-51	3.0387313661194215E-5	3.0387313661194215E-5	0.0	1.163393497175651	0.0011547179191253802
52-53	3.0387313661194215E-5	3.0387313661194215E-5	0.0	1.3412048630641291	0.0013826227715843368
54-55	3.0387313661194215E-5	3.0387313661194215E-5	0.0	1.5593401941810119	0.0013978164284149338
56-57	3.0387313661194215E-5	3.0387313661194215E-5	0.0	1.9053301475273692	0.0014433973989067253
58-59	3.0387313661194215E-5	3.0387313661194215E-5	0.0	2.179833945485767	0.0014889783693985165
60-61	3.0387313661194215E-5	3.0387313661194215E-5	0.0	2.4595947487075516	0.0014889783693985165
62-63	3.0387313661194215E-5	3.0387313661194215E-5	0.0	2.7410420478375324	0.0016409149377044877
64-65	3.0387313661194215E-5	3.0387313661194215E-5	0.0	3.0163511096079523	0.0016561085945350847
66-67	3.0387313661194215E-5	3.0387313661194215E-5	0.0	3.3771548783641414	0.001671302251365682
68-69	3.0387313661194215E-5	3.0387313661194215E-5	0.0	3.803990279706106	0.001671302251365682
70-71	3.0387313661194215E-5	3.0387313661194215E-5	0.0	4.216953872361735	0.0016864959081962791
72-73	3.0387313661194215E-5	3.0387313661194215E-5	0.0	4.572698153393336	0.0017320768786880701
74-75	3.0387313661194215E-5	4.558097049179132E-5	0.0	5.006704960759342	0.0017624641923492644
76-77	3.0387313661194215E-5	6.077462732238843E-5	0.0	5.401269034993119	0.0017624641923492644
78-79	3.0387313661194215E-5	6.077462732238843E-5	0.0	6.092094223766708	0.0018232388196716528
80-81	3.0387313661194215E-5	6.077462732238843E-5	0.0	6.7604935750583355	0.001853626133332847
82-83	3.0387313661194215E-5	6.077462732238843E-5	0.0	7.264755851609023	0.001975175387977624
84-85	3.0387313661194215E-5	6.077462732238843E-5	0.0	7.809038220251503	0.002005562701638818
86-87	3.0387313661194215E-5	6.077462732238843E-5	0.0	8.329618484238253	0.002111918299452998
88	3.0387313661194215E-5	6.077462732238843E-5	0.0	8.796884206406435	0.002218273897267178
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGC	65	0.0061629475	28.916216	9
GTACGCA	2305	0.0	27.531055	3
GAGTACG	2525	0.0	26.075434	1
AGTACGC	2520	0.0	25.36871	2
GTACTAG	840	0.0	25.194056	1
TACGCAG	2530	0.0	25.082258	4
TAGGCAT	1145	0.0	23.39966	5
GTGATCG	525	0.0	23.270672	8
TATACCG	440	0.0	22.434006	5
TGATCGC	560	0.0	21.816254	9
GCTTATG	1315	0.0	21.815691	1
TACCGAC	325	1.8189894E-12	21.687492	7
CGGGCGT	540	0.0	20.883615	6
TACGGGC	565	0.0	20.79912	4
CTAGGCA	1475	0.0	20.395828	4
ATACCGA	415	0.0	20.380398	6
GCATCGT	930	0.0	20.210258	8
GTATAAC	495	0.0	19.951656	1
TCTAACG	355	3.6379788E-12	19.861996	2
CGCAGTG	3235	0.0	19.7539	6
>>END_MODULE