FastQCFastQC Report
Mon 16 Jan 2017
ERR1146620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290847
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2330017.080274470371914No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC642131.9512605721262641No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT539231.6385751145525755No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC457311.3896422410400726No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA430001.3066544874313513No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA387941.1788454461723683No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG371341.128402505494786No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC344691.0474203145877035No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG217820.6618964661681324No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT187130.5686378005419274No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG114310.3473573824611111No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA70090.21298468145131028No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55720.1693181117201742No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC50860.15454987728083378No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT48970.14880667499886807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39090.11878400910160819No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC38400.1166872844589858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.11574527773548877No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36930.11222034935079023No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATG13850.026.140911
GTACTAG6700.024.5625231
GTACGCA26900.024.2960473
GAGTACG27850.023.6364671
TACGCAG27600.023.5094874
AGTACGC28700.022.9364322
TGATCGC4400.022.4309849
CTAACGC3401.8189894E-1220.7436643
GAGTACT52250.020.28314812-13
GTGATCG5150.019.1643358
CATGGGG40000.019.1602294
GTACTTT54650.019.13440514-15
TAACGCC3707.2759576E-1219.0617474
CGACGAG3700.019.05305724-25
TATGTAC17800.019.018914
AGTACTT54800.018.86762612-13
GTACCCG3255.6024874E-1018.8078751
TCTAACG3507.4578566E-1118.8078752
TAGTACT9600.018.6116754
TACCTGG18500.018.5537152
Produced by FastQC (version 0.11.5)