##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3290847
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.315361972160964	33.0	31.0	34.0	30.0	34.0
2	32.3156336347451	33.0	31.0	34.0	30.0	34.0
3	32.511752445495034	34.0	31.0	34.0	31.0	34.0
4	35.86878241376764	37.0	35.0	37.0	35.0	37.0
5	35.94544444029151	37.0	35.0	37.0	35.0	37.0
6	36.12768506101924	37.0	35.0	37.0	35.0	37.0
7	36.15960237592328	37.0	36.0	37.0	35.0	37.0
8	36.17475045178339	37.0	37.0	37.0	35.0	37.0
9	37.95870546397326	39.0	38.0	39.0	35.0	39.0
10-11	37.90862975398127	39.0	38.0	39.0	35.0	39.0
12-13	37.91115114133231	39.0	38.0	39.0	35.0	39.0
14-15	39.073468775667784	40.0	38.0	41.0	36.0	41.0
16-17	39.17944149332983	40.0	39.0	41.0	36.0	41.0
18-19	39.35718418388944	41.0	39.0	41.0	36.0	41.0
20-21	39.38627988478346	41.0	39.0	41.0	36.0	41.0
22-23	39.32495494321067	41.0	39.0	41.0	36.0	41.0
24-25	39.24040528167976	40.5	39.0	41.0	36.0	41.0
26-27	39.07387125563723	40.0	38.5	41.0	35.5	41.0
28-29	39.10077040956325	40.0	38.5	41.0	36.0	41.0
30-31	39.13813708142615	40.5	39.0	41.0	36.0	41.0
32-33	39.041946343904776	40.0	39.0	41.0	35.0	41.0
34-35	38.866586018736214	40.0	38.0	41.0	35.0	41.0
36-37	38.734197305435345	40.0	38.0	41.0	35.0	41.0
38-39	38.66090918234728	40.0	38.0	41.0	35.0	41.0
40-41	38.81044636836656	40.0	38.0	41.0	35.0	41.0
42-43	38.7498441890492	40.0	38.0	41.0	35.0	41.0
44-45	38.62170271057877	40.0	38.0	41.0	35.0	41.0
46-47	38.419674934750844	40.0	38.0	41.0	34.0	41.0
48-49	38.12553272759262	40.0	38.0	41.0	33.5	41.0
50-51	37.948222448506414	40.0	37.0	41.0	33.0	41.0
52-53	38.089712466121945	40.0	38.0	41.0	34.0	41.0
54-55	37.98467218317959	40.0	37.5	41.0	33.0	41.0
56-57	37.7142220528636	40.0	37.0	41.0	32.5	41.0
58-59	37.50583527584236	39.0	36.0	41.0	32.0	41.0
60-61	36.99365360954186	39.0	35.5	41.0	31.0	41.0
62-63	36.95977433773129	39.0	35.0	40.5	31.5	41.0
64-65	36.68726227624681	38.5	35.0	39.5	31.5	41.0
66-67	36.222607887878105	37.5	35.0	39.0	31.0	41.0
68-69	35.67965967424192	37.0	35.0	39.0	30.5	41.0
70-71	35.036244924179094	36.0	34.0	38.0	29.5	40.0
72-73	34.584502409258164	35.0	34.0	37.0	29.5	39.0
74-75	34.359924359898834	35.0	34.0	37.0	29.5	39.0
76-77	34.11263513618226	35.0	34.0	36.5	30.0	39.0
78-79	33.80744303822086	35.0	34.0	36.0	29.0	37.0
80-81	33.46860261203271	35.0	34.0	36.0	29.0	37.0
82-83	33.13975155940097	35.0	33.5	35.0	28.5	36.5
84-85	32.99812981278072	35.0	33.0	35.0	29.0	36.0
86-87	33.10891709641925	35.0	33.5	35.0	29.0	36.0
88-89	32.932607623508474	35.0	34.0	35.0	29.0	36.0
90-91	32.75653790650249	35.0	33.0	35.0	28.0	35.0
92-93	32.63606162790309	35.0	33.0	35.0	27.0	35.0
94-95	32.51156161316524	35.0	33.0	35.0	27.0	35.0
96-97	32.43911795352382	35.0	33.0	35.0	26.5	35.0
98-99	32.529398510474664	35.0	33.0	35.0	27.0	35.0
100	31.56627579465104	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2495408870838176
1101	2	0.266394442735681
1101	3	0.28198923602096926
1101	4	0.19359339159115052
1101	5	0.20330558906432827
1101	6	0.2497503339695868
1101	7	0.18475787838049484
1101	8	0.24922042701371083
1101	9	0.26522039691941046
1101	10-11	0.26001683933502306
1101	12-13	0.26985324378188125
1101	14-15	0.4668865313241213
1101	16-17	0.4316270260851667
1101	18-19	0.3910826386214623
1101	20-21	0.3752131353314425
1101	22-23	0.38861966077224963
1101	24-25	0.3375900083790242
1101	26-27	0.4107492259327046
1101	28-29	0.41502581502321334
1101	30-31	0.44377375750394776
1101	32-33	0.38878270190684106
1101	34-35	0.4256301305315091
1101	36-37	0.41983190561578
1101	38-39	0.42900571952932864
1101	40-41	0.49405244813095095
1101	42-43	0.5250761742875198
1101	44-45	0.485622321637706
1101	46-47	0.5461438776681504
1101	48-49	0.5883641211690858
1101	50-51	0.5641726642299929
1101	52-53	0.4677767716415815
1101	54-55	0.5077219687753001
1101	56-57	0.4986933104812721
1101	58-59	0.5770741472148941
1101	60-61	0.5047754033493774
1101	62-63	0.49793102945308476
1101	64-65	0.4818386075450434
1101	66-67	0.5102920645938482
1101	68-69	0.43811552968311673
1101	70-71	0.5343894768714179
1101	72-73	0.45914350960994454
1101	74-75	0.45519538483599575
1101	76-77	0.4122480798017989
1101	78-79	0.40051949288307753
1101	80-81	0.4362537968354445
1101	82-83	0.46633219308254326
1101	84-85	0.4307686808011937
1101	86-87	0.4197137952945127
1101	88-89	0.46019231582464215
1101	90-91	0.41454908406593205
1101	92-93	0.455540604091901
1101	94-95	0.47215333519425684
1101	96-97	0.4460541839008201
1101	98-99	0.54234464517927
1101	100	0.7194584295695208
1102	1	0.2764532588802524
1102	2	0.20472675753816816
1102	3	0.2391966354795514
1102	4	0.17785556565345928
1102	5	0.17449666279439668
1102	6	0.21611472053417202
1102	7	0.2328481095830881
1102	8	0.18284875908697273
1102	9	0.25310138452069
1102	10-11	0.28978441966216906
1102	12-13	0.26338379454833216
1102	14-15	0.44506061896123583
1102	16-17	0.5001974953660238
1102	18-19	0.4027659711262146
1102	20-21	0.3167751622187822
1102	22-23	0.33876956185828533
1102	24-25	0.35444451503801133
1102	26-27	0.31620932711667393
1102	28-29	0.35816769816872807
1102	30-31	0.38066395808026243
1102	32-33	0.32696716167432527
1102	34-35	0.3264758003605479
1102	36-37	0.3981526757658074
1102	38-39	0.4033857308887221
1102	40-41	0.43188471157696284
1102	42-43	0.4475379629652707
1102	44-45	0.38003140216464715
1102	46-47	0.4652983626120317
1102	48-49	0.541825752259868
1102	50-51	0.517700718696922
1102	52-53	0.4133373919714245
1102	54-55	0.4155759496753859
1102	56-57	0.452900606992408
1102	58-59	0.46783891184750104
1102	60-61	0.4521144172395424
1102	62-63	0.48389714192327205
1102	64-65	0.4010702151291028
1102	66-67	0.35495188310618886
1102	68-69	0.3972093706122024
1102	70-71	0.41902016290001853
1102	72-73	0.4243300977395208
1102	74-75	0.35737705564244493
1102	76-77	0.4153001254776285
1102	78-79	0.4642814784038407
1102	80-81	0.48628138628012607
1102	82-83	0.4287113529564053
1102	84-85	0.41858345021769594
1102	86-87	0.46695218920190484
1102	88-89	0.4302935009630602
1102	90-91	0.4386196433964855
1102	92-93	0.43651825066185523
1102	94-95	0.47096800330484
1102	96-97	0.5066056280728262
1102	98-99	0.5126654143912788
1102	100	0.4454904952345089
1103	1	0.15919047253223084
1103	2	0.1819234268236798
1103	3	0.19275676458167368
1103	4	0.17276429526449277
1103	5	0.1726228815318933
1103	6	0.16546600270795864
1103	7	0.15961868523417166
1103	8	0.13748672990450928
1103	9	0.17867534131678298
1103	10-11	0.1961003332331117
1103	12-13	0.2169938012016246
1103	14-15	0.3281708636111773
1103	16-17	0.42270752174915316
1103	18-19	0.34280392009053173
1103	20-21	0.2990305874807575
1103	22-23	0.2843777605332889
1103	24-25	0.3124200239438579
1103	26-27	0.2761642338290642
1103	28-29	0.25979843972736205
1103	30-31	0.3145437436709386
1103	32-33	0.33794984454246446
1103	34-35	0.30637393071546626
1103	36-37	0.3012504366508395
1103	38-39	0.26508252536578425
1103	40-41	0.27074046304475274
1103	42-43	0.28788030876290094
1103	44-45	0.28624197567464904
1103	46-47	0.3296873836229821
1103	48-49	0.31601343385396063
1103	50-51	0.338269767365027
1103	52-53	0.28457965486835946
1103	54-55	0.30303643471079766
1103	56-57	0.36177769410802796
1103	58-59	0.3114981700942181
1103	60-61	0.2749537239349422
1103	62-63	0.3400382849973056
1103	64-65	0.3410342878092152
1103	66-67	0.26734213037497767
1103	68-69	0.23649599334238758
1103	70-71	0.2736965538090743
1103	72-73	0.25219625426169046
1103	74-75	0.21777338421230752
1103	76-77	0.23414403019756946
1103	78-79	0.29477964344079766
1103	80-81	0.1950375175693395
1103	82-83	0.25753646346116454
1103	84-85	0.23033368334823479
1103	86-87	0.22607071241940702
1103	88-89	0.30251268924406816
1103	90-91	0.2585546610206322
1103	92-93	0.2445227460558712
1103	94-95	0.24547618410797867
1103	96-97	0.21902770407603356
1103	98-99	0.1690944648465731
1103	100	0.25292853205409216
1104	1	0.2195110994547278
1104	2	0.19190255679328772
1104	3	0.17611803478416732
1104	4	0.1645643840323885
1104	5	0.12507429933187098
1104	6	0.13140788598960285
1104	7	0.09755296919321665
1104	8	0.10390658228541838
1104	9	0.1418167426449628
1104	10-11	0.12318031730801948
1104	12-13	0.17077828176889653
1104	14-15	0.29633989757616774
1104	16-17	0.31528755725013013
1104	18-19	0.25688631053522215
1104	20-21	0.19979107569800902
1104	22-23	0.2598713813831779
1104	24-25	0.2461864019141018
1104	26-27	0.272859065925239
1104	28-29	0.26720464427590684
1104	30-31	0.2821932661496902
1104	32-33	0.29876548993046725
1104	34-35	0.3036053391073992
1104	36-37	0.2691498311821263
1104	38-39	0.21562283893204182
1104	40-41	0.23115273031086758
1104	42-43	0.2620844940734557
1104	44-45	0.2875330327569756
1104	46-47	0.3458952462841509
1104	48-49	0.3759590982649925
1104	50-51	0.3158157211210906
1104	52-53	0.34572248130118055
1104	54-55	0.3374238790880071
1104	56-57	0.29503513922961133
1104	58-59	0.34602452665391326
1104	60-61	0.37317459785704443
1104	62-63	0.3482101667006532
1104	64-65	0.305212550052552
1104	66-67	0.33903903959049586
1104	68-69	0.35072443075870297
1104	70-71	0.3893432236076322
1104	72-73	0.3318615801439506
1104	74-75	0.3212320736287637
1104	76-77	0.2514179415476576
1104	78-79	0.3041645165141418
1104	80-81	0.3354629857396887
1104	82-83	0.3118657979150612
1104	84-85	0.31201960622394864
1104	86-87	0.33034272146792887
1104	88-89	0.3132410492896227
1104	90-91	0.3276291491458352
1104	92-93	0.35102599825336966
1104	94-95	0.3254011113006996
1104	96-97	0.29817614382737645
1104	98-99	0.26638271990262297
1104	100	0.3398586793797662
1105	1	0.10239923150462715
1105	2	0.134811977332852
1105	3	0.13206153413708677
1105	4	0.11020124872795378
1105	5	0.05645079546088283
1105	6	0.11710511274264235
1105	7	0.10283034174944561
1105	8	0.117808616944302
1105	9	0.15832479857701998
1105	10-11	0.13187279818283315
1105	12-13	0.16735029889157715
1105	14-15	0.298151070395825
1105	16-17	0.34699352721651877
1105	18-19	0.21584013537996327
1105	20-21	0.21777953837040087
1105	22-23	0.2404221349038238
1105	24-25	0.2496685871622546
1105	26-27	0.2503934874220164
1105	28-29	0.2197340643177128
1105	30-31	0.23531403046773391
1105	32-33	0.2763823038169946
1105	34-35	0.2649892249459569
1105	36-37	0.2903759582085499
1105	38-39	0.21195212994110335
1105	40-41	0.2583209447962318
1105	42-43	0.3307366665714824
1105	44-45	0.3103642200114507
1105	46-47	0.31783768216980945
1105	48-49	0.49469081543438875
1105	50-51	0.4632628732112707
1105	52-53	0.377405238773207
1105	54-55	0.4317150652321189
1105	56-57	0.41262767332565176
1105	58-59	0.42843216007072016
1105	60-61	0.4084006109747378
1105	62-63	0.42203135487405063
1105	64-65	0.40173486372084
1105	66-67	0.38737324399063056
1105	68-69	0.39665248064115133
1105	70-71	0.420370024705754
1105	72-73	0.393613132748726
1105	74-75	0.3805237718835599
1105	76-77	0.2789690186073983
1105	78-79	0.3550010756662729
1105	80-81	0.34980797222379323
1105	82-83	0.367382713840847
1105	84-85	0.3180339635106648
1105	86-87	0.32779714840170016
1105	88-89	0.3730712056221819
1105	90-91	0.32721039096163906
1105	92-93	0.3880828071240785
1105	94-95	0.39423728469468955
1105	96-97	0.34135901712257777
1105	98-99	0.35077494979533697
1105	100	0.39520588086230646
1106	1	0.0944946642128528
1106	2	0.1265616260310054
1106	3	0.13191333442107123
1106	4	0.1218774826986575
1106	5	0.09911493250906744
1106	6	0.08137022056104115
1106	7	0.12904808083701624
1106	8	0.09528980114418317
1106	9	0.100346108436284
1106	10-11	0.12087668273511554
1106	12-13	0.11608487655688293
1106	14-15	0.25775952058452845
1106	16-17	0.28480767566821186
1106	18-19	0.18478116725095362
1106	20-21	0.15188536023278232
1106	22-23	0.1365204313657884
1106	24-25	0.11856823530862926
1106	26-27	0.19267266386751203
1106	28-29	0.17726417787465465
1106	30-31	0.20329493693684242
1106	32-33	0.17413381110421255
1106	34-35	0.1936500292248482
1106	36-37	0.20903794882088533
1106	38-39	0.2569564476430415
1106	40-41	0.16226773360313018
1106	42-43	0.12766778750024343
1106	44-45	0.11680899940860456
1106	46-47	0.1827838511404778
1106	48-49	0.23916107617024096
1106	50-51	0.1702577802542251
1106	52-53	0.2514610920872187
1106	54-55	0.30450092101075654
1106	56-57	0.20743424579683278
1106	58-59	0.269634233119433
1106	60-61	0.2852423936384483
1106	62-63	0.25562620269229797
1106	64-65	0.30089075069360405
1106	66-67	0.23958833084127207
1106	68-69	0.20909808403425245
1106	70-71	0.19738883774696347
1106	72-73	0.2658418946212606
1106	74-75	0.2419708779656844
1106	76-77	0.24556545556067277
1106	78-79	0.17258644569195525
1106	80-81	0.227952813420643
1106	82-83	0.18277387280584634
1106	84-85	0.16517115239702207
1106	86-87	0.15672800447462265
1106	88-89	0.2555828804307083
1106	90-91	0.15368316874758392
1106	92-93	0.12939047764341893
1106	94-95	0.27447841846159093
1106	96-97	0.23298076712073623
1106	98-99	0.2546414541764932
1106	100	0.2728231715342275
1107	1	0.11564349217211856
1107	2	0.1041847875373847
1107	3	0.1447516911188984
1107	4	0.1058427463861733
1107	5	0.062425474770513745
1107	6	0.09610425481046292
1107	7	0.11645406204556963
1107	8	0.09144707676717445
1107	9	0.11994366445182436
1107	10-11	0.12605342798060093
1107	12-13	0.13969250771623365
1107	14-15	0.19732706430216496
1107	16-17	0.2898483190465555
1107	18-19	0.16829416766123728
1107	20-21	0.17455852910401148
1107	22-23	0.16977587095567515
1107	24-25	0.1829777817851621
1107	26-27	0.2042924025065176
1107	28-29	0.16175446313927466
1107	30-31	0.21776963202748334
1107	32-33	0.20962040877609667
1107	34-35	0.17126157255058416
1107	36-37	0.20430903769948117
1107	38-39	0.24185207832128697
1107	40-41	0.2155643790070414
1107	42-43	0.23208692053379565
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2216	56-57	-1.0450542592286496
2216	58-59	-1.0938989167914528
2216	60-61	-1.1461319434542432
2216	62-63	-1.1047301805625764
2216	64-65	-0.8944639588223495
2216	66-67	-0.9908295567703433
2216	68-69	-0.9749296331216613
2216	70-71	-0.9719259728976155
2216	72-73	-0.9508655872070548
2216	74-75	-0.8795671777872371
2216	76-77	-0.8926964922571443
2216	78-79	-0.8644493802777404
2216	80-81	-1.0292602187731745
2216	82-83	-1.0470592994336059
2216	84-85	-1.0559371235013089
2216	86-87	-0.9555291106255197
2216	88-89	-0.9030449239286185
2216	90-91	-1.0876276339983733
2216	92-93	-1.103519227619067
2216	94-95	-1.0959271879407027
2216	96-97	-1.1201484045884378
2216	98-99	-1.0789577111800028
2216	100	-1.281772002133323
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	10.0
8	77.0
9	144.0
10	190.0
11	108.0
12	109.0
13	153.0
14	227.0
15	414.0
16	694.0
17	1304.0
18	2413.0
19	3427.0
20	4964.0
21	7433.0
22	10780.0
23	13647.0
24	15317.0
25	16460.0
26	19025.0
27	23014.0
28	29306.0
29	36198.0
30	44890.0
31	56712.0
32	72563.0
33	95292.0
34	135769.0
35	213646.0
36	422523.0
37	917301.0
38	957725.0
39	188543.0
40	469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.585718045165336	17.624471473710887	13.315762892030216	19.474047589093562
2	13.399576564385521	26.98926459742164	31.450228993761513	28.16092984443132
3	30.491424681844148	22.00164699590378	30.29484873163083	17.21207959062124
4	11.001147102433846	14.868369007621101	44.51049311419309	29.619990775751965
5	10.560302519966525	43.0778562264378	34.43425496496552	11.927586288630158
6	27.094407579251385	38.18437472270068	15.148687135864208	19.572530562183726
7	24.570308060602045	33.44559423321541	23.007913466628338	18.976184239554208
8	23.736414538077582	23.614591316365395	20.212566176233747	32.436427969323276
9	19.47725856262362	23.545395511119796	27.16829699867177	29.80904892758482
10-11	27.009717727213538	21.404464481197287	27.064278240079325	24.52153955150985
12-13	34.062613667545165	21.351691525008608	24.09344767471718	20.49224713272905
14-15	24.39539826257337	24.645744374391494	32.11129120675425	18.84756615628088
16-17	19.78111337058425	27.4059995697142	30.333036954743413	22.479850104958132
18-19	18.311634245915563	35.341454854300345	25.50632132476613	20.84058957501797
20-21	23.518464271453933	25.361761518784554	22.813355973942233	28.30641823581928
22-23	20.37522558781979	26.348140767407298	25.978433515748378	27.298200129024536
24-25	27.75103187720365	25.93441749191014	23.673844454026575	22.640706176859634
26-27	24.857695993912802	20.52339492446006	33.64869841821637	20.970210663410764
28-29	19.42986106616321	34.12764251878012	26.029058780307928	20.413437634748743
30-31	22.383077760365815	24.68212532905722	25.336730033447374	27.598066877129586
32-33	23.81885274458333	26.0818791817825	27.506927190092856	22.59234088354131
34-35	28.124517600955862	23.602258265660726	26.562076476429752	21.71114765695366
36-37	24.517696136144952	21.50062954945773	33.25303482038279	20.72863949401453
38-39	22.278903554286707	27.581997164836064	29.686612586851584	20.452486694025644
40-41	21.58412923183513	29.382632952780906	26.80176429654525	22.231473518838712
42-43	20.318446892243852	29.230043207721295	21.74975014031342	28.701759759721433
44-45	23.695139616710794	23.222113198763473	24.45988566413772	28.62286152038801
46-47	28.22931300057402	27.606676943656144	25.851855160692672	18.312154895077164
48-49	25.390108830727186	25.921203508704227	26.8459205532197	21.84276710734889
50-51	16.626707349202196	27.91822287696754	33.97499184860311	21.480077925227153
52-53	22.65429452056668	30.91712645400116	23.15465415595383	23.273924869478336
54-55	19.582367456531934	24.593526476786824	27.294299578328967	28.52980648835227
56-57	25.988132218421274	21.829729013163043	26.353935200484376	25.82820356793131
58-59	31.428961691345904	22.89342210140138	24.164259214184415	21.5133569930683
60-61	23.688441186431433	25.55149899273025	32.51964210206767	18.240417718770647
62-63	20.293943053775152	26.5161205728079	30.013387153407418	23.17654922000953
64-65	18.593697557960585	34.14698182270126	25.007311972901714	22.252008646436444
66-67	23.230712686346628	25.18603705258539	22.746698038886784	28.8365522221812
68-69	20.23425495555984	26.34935221156971	25.922400918811338	27.493991914059112
70-71	27.071444861726413	25.812776789904884	23.31947778926962	23.796300559099084
72-73	24.225290351831756	20.80565852941078	32.776975697629936	22.19207542112753
74-75	19.629587029985025	33.26367362914245	25.37538826523178	21.731351075640745
76-77	22.260496526671446	24.30758683625268	25.103355056546146	28.328561580529726
78-79	23.358474609620416	25.60177172535522	27.439772687734166	23.599980977290198
80-81	27.017293135179756	23.72909503103259	26.171560262664457	23.082051571123195
82-83	23.459265767814188	21.81951002871468	32.24418918596565	22.477035017505486
84-85	21.522847513931524	27.376601363456043	28.66703627976967	22.433514842842765
86-87	21.22765051064361	28.96456444192027	26.22455556274722	23.583229484688896
88-89	20.117541167972867	28.309231635502957	22.070321105782188	29.502906090741988
90-91	23.075597722348945	22.811926961649533	24.67395547011992	29.4385198458816
92-93	26.766641065319902	27.149020349652105	25.742480838873327	20.34185774615467
94-95	23.706578008559507	25.513956298141142	26.799447193485925	23.980018499813422
96-97	16.39337238318774	26.907250923473548	33.10051002237729	23.59886667096142
98-99	22.07526041983202	29.536622868325413	23.556705886642924	24.831410825199647
100	21.366208524867744	19.726969950942053	24.532562776049797	34.374258748140406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	89.5
2	111.0
3	196.5
4	139.0
5	102.0
6	185.5
7	449.5
8	789.0
9	987.0
10	815.5
11	576.0
12	609.0
13	684.5
14	783.5
15	901.0
16	1076.5
17	1290.0
18	1493.0
19	1720.5
20	2030.0
21	2396.0
22	2865.0
23	3447.5
24	4349.0
25	5511.5
26	6903.0
27	8910.0
28	11091.5
29	14062.0
30	18012.0
31	21668.0
32	25983.5
33	30029.5
34	33125.5
35	36741.5
36	40559.5
37	44538.0
38	47386.5
39	48037.0
40	47745.5
41	47589.0
42	48514.5
43	51894.0
44	58841.5
45	67990.0
46	89887.5
47	262522.5
48	614981.5
49	598743.0
50	282465.5
51	141023.5
52	90192.5
53	71792.0
54	62678.0
55	55898.0
56	50228.5
57	44149.0
58	37994.5
59	33927.5
60	28394.5
61	21965.0
62	16946.5
63	12876.5
64	9092.0
65	6080.0
66	4292.5
67	3123.0
68	2181.0
69	1550.5
70	1136.0
71	865.0
72	703.5
73	626.0
74	478.0
75	313.0
76	207.5
77	120.5
78	67.5
79	27.5
80	11.5
81	5.5
82	2.0
83	3.0
84	2.0
85	0.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002826020170491062
2	0.004436547794534356
3	2.1271119562835952E-4
4	0.051354560087418226
5	0.002704470915846285
6	0.005439329145353765
7	3.0387313661194215E-5
8	3.9503507759552486E-4
9	1.2154925464477686E-4
10-11	1.671302251365682E-4
12-13	0.0
14-15	9.116194098358264E-5
16-17	3.342604502731364E-4
18-19	6.077462732238843E-5
20-21	1.5193656830597108E-4
22-23	0.0
24-25	0.0
26-27	1.5193656830597108E-4
28-29	0.0
30-31	1.8232388196716528E-4
32-33	3.190667934425393E-4
34-35	9.116194098358264E-5
36-37	4.558097049179132E-5
38-39	9.723940371582149E-4
40-41	5.165843322403016E-4
42-43	0.0
44-45	4.8619701857910744E-4
46-47	0.0
48-49	7.596828415298554E-5
50-51	0.0
52-53	4.2542239125671903E-4
54-55	3.494541071037335E-4
56-57	1.3674291147537396E-4
58-59	7.292955278686611E-4
60-61	6.229399300544813E-4
62-63	1.671302251365682E-4
64-65	3.494541071037335E-4
66-67	0.001139524262294783
68-69	0.002233467554097775
70-71	0.003236248904917184
72-73	0.002977956738797033
74-75	0.002658889945354494
76-77	0.003874382491802262
78-79	0.0013218481442619483
80-81	9.87587693988812E-4
82-83	6.533272437156757E-4
84-85	5.62165302732093E-4
86-87	0.0
88-89	0.0
90-91	1.5193656830597108E-5
92-93	1.3674291147537396E-4
94-95	9.116194098358264E-5
96-97	3.342604502731364E-4
98-99	2.1271119562835952E-4
100	0.0756947983300348
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3290847.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.40413129122052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.11893665482107	36.61781677660437
2	14.663202827388952	14.488455952682394
3	5.276744136200013	7.820788802850104
4	2.4222074364333075	4.786682168164872
5	1.2771485903340567	3.1548208317630473
6	0.7238406804789627	2.145643200738544
7	0.42691345580399	1.4763901894370293
8	0.2920415802753121	1.1542448459533627
9	0.19459421937208485	0.8652382526133942
>10	0.5875721519291383	4.270876871797554
>50	0.010126470374673967	0.33849960587824784
>100	0.004589302757573039	0.4446725333578503
>500	4.164987661774886E-4	0.13145120005509553
>1k	6.941646102949778E-4	1.046636883505676
>5k	2.0824938308849333E-4	0.6058826901567804
>10k+	7.635810713244756E-4	20.65189919444171
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	233001	7.080274470371914	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	64213	1.9512605721262641	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	53923	1.6385751145525755	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	45731	1.3896422410400726	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	43000	1.3066544874313513	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	38794	1.1788454461723683	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	37134	1.128402505494786	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	34469	1.0474203145877035	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	21782	0.6618964661681324	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	18713	0.5686378005419274	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	11431	0.3473573824611111	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	7009	0.21298468145131028	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	5572	0.1693181117201742	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	5086	0.15454987728083378	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	4897	0.14880667499886807	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3909	0.11878400910160819	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	3840	0.1166872844589858	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3809	0.11574527773548877	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	3693	0.11222034935079023	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.116194098358264E-5
2	0.0	0.0	0.0	0.0	1.2154925464477686E-4
3	0.0	0.0	0.0	0.0	1.2154925464477686E-4
4	0.0	0.0	0.0	0.0	1.2154925464477686E-4
5	0.0	0.0	0.0	0.0	1.2154925464477686E-4
6	0.0	0.0	0.0	3.0387313661194215E-5	1.2154925464477686E-4
7	0.0	0.0	0.0	6.077462732238843E-5	1.2154925464477686E-4
8	0.0	0.0	0.0	9.116194098358264E-5	1.2154925464477686E-4
9	0.0	0.0	0.0	1.2154925464477686E-4	1.2154925464477686E-4
10-11	0.0	0.0	0.0	3.0387313661194216E-4	1.2154925464477686E-4
12-13	0.0	0.0	0.0	6.077462732238843E-4	1.975175387977624E-4
14-15	0.0	0.0	0.0	9.116194098358264E-4	3.190667934425393E-4
16-17	0.0	0.0	0.0	0.0014585910557373223	4.102287344261219E-4
18-19	0.0	0.0	0.0	0.002127111956283595	4.8619701857910744E-4
20-21	0.0	0.0	0.0	0.004056706373769428	5.317779890708987E-4
22-23	0.0	0.0	0.0	0.006290173927867203	5.925526163932872E-4
24-25	0.0	0.0	0.0	0.00872115902076274	6.077462732238843E-4
26-27	0.0	0.0	0.0	0.010529204183603796	6.077462732238843E-4
28-29	0.0	0.0	0.0	0.01753347998250906	6.685209005462728E-4
30-31	0.0	0.0	0.0	0.03166358083496437	6.837145573768699E-4
32-33	0.0	0.0	0.0	0.07578596027101837	6.989082142074669E-4
34-35	0.0	0.0	0.0	0.1308781599387635	8.508447825134381E-4
36-37	0.0	0.0	0.0	0.1916831745748131	8.508447825134381E-4
38-39	0.0	0.0	0.0	0.2842429319868107	9.420067234970207E-4
40-41	0.0	0.0	0.0	0.38587330252667473	9.420067234970207E-4
42-43	0.0	0.0	0.0	0.5007829291364807	0.001002781350819409
44-45	0.0	0.0	0.0	0.6452290246249675	0.0010635559781417975
46-47	0.0	0.0	0.0	0.8342381155975953	0.0010939432918029917
48-49	0.0	0.0	0.0	0.9847312865046598	0.001109136948633589
50-51	0.0	0.0	0.0	1.1620564553745587	0.0011851052327865744
52-53	0.0	0.0	0.0	1.3408554089570255	0.0013978164284149338
54-55	0.0	0.0	0.0	1.5598111975427602	0.0014585910557373223
56-57	0.0	0.0	0.0	1.90763958336562	0.0014737847125679193
58-59	0.0	0.0	0.0	2.18296383879287	0.0014889783693985165
60-61	0.0	0.0	0.0	2.463773004335966	0.0014889783693985165
62-63	0.0	3.0387313661194215E-5	0.0	2.746420602355564	0.0015801403103820992
64-65	0.0	3.0387313661194215E-5	0.0	3.023188255181721	0.0015953339672126962
66-67	0.0	3.0387313661194215E-5	0.0	3.3856785198461066	0.0016105276240432934
68-69	0.0	3.0387313661194215E-5	0.0	3.8155070715836987	0.0016105276240432934
70-71	0.0	3.0387313661194215E-5	0.0	4.230369871343153	0.0016409149377044877
72-73	0.0	3.0387313661194215E-5	0.0	4.588302038958359	0.0017928515060104586
74-75	0.0	3.0387313661194215E-5	0.0	5.02445115193748	0.0018992071038246385
76-77	0.0	3.0387313661194215E-5	0.0	5.421187919098031	0.0019144007606552355
78-79	0.0	3.0387313661194215E-5	0.0	6.113471698927358	0.0019144007606552355
80-81	0.0	3.0387313661194215E-5	0.0	6.783010574481281	0.0019295944174858326
82-83	0.0	3.0387313661194215E-5	0.0	7.288549118205738	0.0020359500153000125
84-85	0.0	3.0387313661194215E-5	0.0	7.832299708859148	0.0020359500153000125
86-87	0.0	3.0387313661194215E-5	0.0	8.3524849377683	0.002096724642622401
88	0.0	6.077462732238843E-5	0.0	8.821467543158342	0.0021574992699447894
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTATG	1385	0.0	26.14091	1
GTACTAG	670	0.0	24.562523	1
GTACGCA	2690	0.0	24.296047	3
GAGTACG	2785	0.0	23.636467	1
TACGCAG	2760	0.0	23.509487	4
AGTACGC	2870	0.0	22.936432	2
TGATCGC	440	0.0	22.430984	9
CTAACGC	340	1.8189894E-12	20.743664	3
GAGTACT	5225	0.0	20.283148	12-13
GTGATCG	515	0.0	19.164335	8
CATGGGG	4000	0.0	19.160229	4
GTACTTT	5465	0.0	19.134405	14-15
TAACGCC	370	7.2759576E-12	19.061747	4
CGACGAG	370	0.0	19.053057	24-25
TATGTAC	1780	0.0	19.01891	4
AGTACTT	5480	0.0	18.867626	12-13
GTACCCG	325	5.6024874E-10	18.807875	1
TCTAACG	350	7.4578566E-11	18.807875	2
TAGTACT	960	0.0	18.611675	4
TACCTGG	1850	0.0	18.553715	2
>>END_MODULE