FastQCFastQC Report
Mon 16 Jan 2017
ERR1146619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996012
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA102330.2560803120711349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13300.019.8043171
TCTAACG5900.019.125312
TGATCGC7700.018.3075229
CATGGGG68650.018.285144
CTAGTAC19850.018.2378673
ATACGGG8300.017.5601183
GTATATA26800.017.5504671
TAGTACT24200.017.4845584
TACGGGC8400.017.3504184
CTGTGCG18800.017.2460759
CTAACGC6550.017.2271633
GTATAAG20700.016.587311
TACCTGG37350.016.3644732
GGGCTAT21700.016.242176
GTGATCG8400.016.2231088
CTATTGA21500.016.1730619
GGCGTCG8750.016.1112258
GTACATG220750.016.0868031
TAACGCC7400.015.883124
TACATGG221100.015.8209842
Produced by FastQC (version 0.11.5)