##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146619_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3996012
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242289562694005	31.0	31.0	34.0	28.0	34.0
2	31.317408205981362	31.0	31.0	34.0	28.0	34.0
3	31.487277815982534	33.0	31.0	34.0	28.0	34.0
4	34.98592396619429	37.0	35.0	37.0	32.0	37.0
5	35.055693276196365	37.0	35.0	37.0	32.0	37.0
6	35.048311416482235	37.0	35.0	37.0	32.0	37.0
7	35.02683024975901	37.0	35.0	37.0	32.0	37.0
8	35.0337406394175	37.0	35.0	37.0	32.0	37.0
9	36.66853002443436	39.0	37.0	39.0	32.0	39.0
10-11	36.668357227155475	39.0	37.0	39.0	32.0	39.0
12-13	36.629776637307394	39.0	37.0	39.0	32.0	39.0
14-15	37.945046961820935	40.0	38.0	41.0	32.0	41.0
16-17	37.952796938547735	40.0	38.0	41.0	32.0	41.0
18-19	37.94588617351499	40.0	38.0	41.0	32.0	41.0
20-21	38.03390355184119	40.0	38.0	41.0	32.0	41.0
22-23	38.0180407366144	40.0	38.0	41.0	32.0	41.0
24-25	38.01997516523974	40.0	38.0	41.0	32.0	41.0
26-27	38.057686513453916	40.0	38.0	41.0	32.0	41.0
28-29	38.02636616206358	40.0	38.0	41.0	32.0	41.0
30-31	37.98735752044789	40.0	38.0	41.0	32.0	41.0
32-33	37.94159802322916	40.0	38.0	41.0	32.0	41.0
34-35	37.88507567044343	40.0	38.0	41.0	32.0	41.0
36-37	37.815607410588356	40.0	38.0	41.0	31.5	41.0
38-39	37.75954401538334	40.0	38.0	41.0	31.0	41.0
40-41	37.72754498735239	40.0	38.0	41.0	31.0	41.0
42-43	37.569970260349564	40.0	37.5	41.0	31.0	41.0
44-45	37.42649784334982	40.0	37.0	41.0	30.5	41.0
46-47	37.3031733888687	40.0	37.0	41.0	30.0	41.0
48-49	37.196980389448285	40.0	37.0	41.0	30.0	41.0
50-51	37.070077492259784	40.0	36.0	41.0	30.0	41.0
52-53	36.980457516143595	40.0	36.0	41.0	30.0	41.0
54-55	36.86902153947486	39.0	36.0	41.0	30.0	41.0
56-57	36.66240742019794	39.0	35.0	41.0	29.5	41.0
58-59	36.48278859022446	39.0	35.0	41.0	29.0	41.0
60-61	36.247133767366066	39.0	35.0	40.5	29.0	41.0
62-63	36.03550790137767	38.0	35.0	40.0	29.0	41.0
64-65	35.77383351201148	38.0	35.0	40.0	28.0	41.0
66-67	35.514041874749125	37.0	34.0	40.0	28.0	41.0
68-69	35.240165695198115	37.0	34.0	39.5	28.0	41.0
70-71	34.90747712969831	36.0	34.0	39.0	28.0	41.0
72-73	34.55553374214091	36.0	34.0	39.0	27.5	40.5
74-75	34.230038598482686	35.0	34.0	38.5	27.5	40.0
76-77	33.935084279026185	35.0	34.0	37.0	27.0	39.5
78-79	33.601085782525175	35.0	33.0	37.0	26.5	39.0
80-81	33.32328906920199	35.0	33.0	36.5	26.0	39.0
82-83	33.049748098854565	35.0	33.0	36.0	26.0	37.5
84-85	32.842704551437784	35.0	33.0	36.0	26.0	37.0
86-87	32.66027066485286	35.0	33.0	35.0	26.0	37.0
88-89	32.457056185016455	35.0	33.0	35.0	26.0	36.0
90-91	32.291173800278884	35.0	33.0	35.0	25.0	36.0
92-93	32.11868457852479	35.0	32.5	35.0	25.0	36.0
94-95	32.00826961981095	35.0	32.0	35.0	25.0	36.0
96-97	31.92996192203627	35.0	32.0	35.0	25.0	35.0
98-99	31.842509732203006	35.0	32.0	35.0	24.5	35.0
100	30.64699880781139	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2595243190099339
1101	2	0.25043701447148337
1101	3	0.24881516226874467
1101	4	0.24502822601026963
1101	5	0.17122234358888733
1101	6	0.23011810500631924
1101	7	0.25922508451674275
1101	8	0.3204780597751622
1101	9	0.4120644222660701
1101	10-11	0.37435186362910855
1101	12-13	0.39658712740427404
1101	14-15	0.4967544410463063
1101	16-17	0.45936201324862225
1101	18-19	0.4466822620661546
1101	20-21	0.4490395972401373
1101	22-23	0.49759931669347424
1101	24-25	0.48163688561849227
1101	26-27	0.4586568000208686
1101	28-29	0.43220389772310597
1101	30-31	0.4429598333314928
1101	32-33	0.4754153814600457
1101	34-35	0.41043508452514743
1101	36-37	0.4150318366511172
1101	38-39	0.45172125078356373
1101	40-41	0.40309243745964096
1101	42-43	0.4346848688186711
1101	44-45	0.4059336089462704
1101	46-47	0.377696936757836
1101	48-49	0.35690589096850545
1101	50-51	0.34396920989210855
1101	52-53	0.3539886225603226
1101	54-55	0.3297534379779492
1101	56-57	0.35303289220517087
1101	58-59	0.29336105490802566
1101	60-61	0.3755610356910921
1101	62-63	0.2822801036427549
1101	64-65	0.38454407133199453
1101	66-67	0.2997713682722889
1101	68-69	0.3061055589806472
1101	70-71	0.2937571018893692
1101	72-73	0.29420502683449
1101	74-75	0.27741190123038706
1101	76-77	0.3062477784049378
1101	78-79	0.3425500726185504
1101	80-81	0.26901705677160237
1101	82-83	0.26825132233313553
1101	84-85	0.22297758245549915
1101	86-87	0.20901953055589217
1101	88-89	0.22723906749346412
1101	90-91	0.13014561628829568
1101	92-93	0.15511889901718234
1101	94-95	0.16459315278134312
1101	96-97	0.19106846516538667
1101	98-99	0.24842622554966098
1101	100	0.2448560256755492
1102	1	0.28836868319289266
1102	2	0.32444201157358776
1102	3	0.34010239315323787
1102	4	0.34338437732280624
1102	5	0.30272375955228625
1102	6	0.3352619277776512
1102	7	0.37460165774396614
1102	8	0.3505033751274098
1102	9	0.4916369709530599
1102	10-11	0.4767621231203023
1102	12-13	0.41233117965119703
1102	14-15	0.5437060850716904
1102	16-17	0.47251239044618387
1102	18-19	0.4846969624049535
1102	20-21	0.5128956295740537
1102	22-23	0.49660404874419584
1102	24-25	0.5841637677189198
1102	26-27	0.5526811282039574
1102	28-29	0.5424926623716075
1102	30-31	0.5586616835079568
1102	32-33	0.5874821991471038
1102	34-35	0.556335122456943
1102	36-37	0.47358109851246155
1102	38-39	0.4839718197605407
1102	40-41	0.48854323685461765
1102	42-43	0.5549759364692122
1102	44-45	0.5106716085975904
1102	46-47	0.5192869446740431
1102	48-49	0.4264840881950107
1102	50-51	0.39285396201604783
1102	52-53	0.39736811017476015
1102	54-55	0.3760963473233119
1102	56-57	0.4051817521308223
1102	58-59	0.47154921076295153
1102	60-61	0.42677647299053945
1102	62-63	0.4549529819358682
1102	64-65	0.5284373757806122
1102	66-67	0.44934752165811886
1102	68-69	0.42976601873289155
1102	70-71	0.33941868192546565
1102	72-73	0.383553848592733
1102	74-75	0.4543286939437152
1102	76-77	0.4302411721464239
1102	78-79	0.3960275762350278
1102	80-81	0.2876788830109831
1102	82-83	0.3628706908747077
1102	84-85	0.34434947075223477
1102	86-87	0.3486056027535298
1102	88-89	0.338328876326635
1102	90-91	0.33602575180612604
1102	92-93	0.31150878724795916
1102	94-95	0.33761987467294574
1102	96-97	0.3325179958477378
1102	98-99	0.3988085768970713
1102	100	0.3924884689074304
1103	1	0.2343477086961805
1103	2	0.29843071464897974
1103	3	0.24341614669230438
1103	4	0.28875786630339206
1103	5	0.27612681699552155
1103	6	0.2977122624952244
1103	7	0.2551261633211226
1103	8	0.3053694302833989
1103	9	0.4541383061893143
1103	10-11	0.3876998691754352
1103	12-13	0.4033794529842467
1103	14-15	0.4841313881015026
1103	16-17	0.5489938281845355
1103	18-19	0.5417814437661548
1103	20-21	0.502029800444646
1103	22-23	0.5142583841599446
1103	24-25	0.5278570614742009
1103	26-27	0.5238016199232902
1103	28-29	0.5154374034128679
1103	30-31	0.5412198846666669
1103	32-33	0.5552846631948327
1103	34-35	0.5894022138113328
1103	36-37	0.5814765844778904
1103	38-39	0.597314794325527
1103	40-41	0.4975984099177495
1103	42-43	0.5069182027808452
1103	44-45	0.46448193236897595
1103	46-47	0.46516186992834463
1103	48-49	0.44551316633689453
1103	50-51	0.4853085075456818
1103	52-53	0.5070137192021704
1103	54-55	0.4492561784062943
1103	56-57	0.5283079476490471
1103	58-59	0.5602656109880471
1103	60-61	0.5734914407962037
1103	62-63	0.5513864626682761
1103	64-65	0.47542729213150636
1103	66-67	0.5414073121609277
1103	68-69	0.6110701439797026
1103	70-71	0.5211891418744301
1103	72-73	0.5642817230397768
1103	74-75	0.5693797385114223
1103	76-77	0.4566911698889129
1103	78-79	0.47969688666947974
1103	80-81	0.4361947679174776
1103	82-83	0.4126093080544919
1103	84-85	0.43939554466211206
1103	86-87	0.38250614995261856
1103	88-89	0.3748953534723398
1103	90-91	0.4465107701800761
1103	92-93	0.43590727708198784
1103	94-95	0.4673607204583874
1103	96-97	0.4913527103934996
1103	98-99	0.44746640980588026
1103	100	0.605296633175076
1104	1	0.17472009531737598
1104	2	0.14035850872996392
1104	3	0.16466007508275027
1104	4	0.12401734257665709
1104	5	0.10368899956501565
1104	6	0.15754550811168855
1104	7	0.14613697123421332
1104	8	0.13168071056405495
1104	9	0.21171487649777987
1104	10-11	0.20827896344852803
1104	12-13	0.2558865368681893
1104	14-15	0.30977156101507575
1104	16-17	0.28171641856414453
1104	18-19	0.3008150211316192
1104	20-21	0.29554889800915163
1104	22-23	0.36042731870143996
1104	24-25	0.3544607566626965
1104	26-27	0.37074933360891293
1104	28-29	0.3773182051504662
1104	30-31	0.3611962255534209
1104	32-33	0.38478454669161977
1104	34-35	0.40075506862878285
1104	36-37	0.386284337888668
1104	38-39	0.3123920530620481
1104	40-41	0.33613165895598485
1104	42-43	0.342987286739735
1104	44-45	0.3243404339258831
1104	46-47	0.30986454233272553
1104	48-49	0.32987209724478816
1104	50-51	0.2671106248081969
1104	52-53	0.32363646225801546
1104	54-55	0.286066613834997
1104	56-57	0.31394539943871536
1104	58-59	0.26107319819843866
1104	60-61	0.27801308513716094
1104	62-63	0.32025683777602154
1104	64-65	0.32333354208059006
1104	66-67	0.28269195964502813
1104	68-69	0.27252265384989016
1104	70-71	0.3278302321489406
1104	72-73	0.306856704668121
1104	74-75	0.3233093836084322
1104	76-77	0.24884838567403733
1104	78-79	0.2776224777806888
1104	80-81	0.2761717116879723
1104	82-83	0.2527792072099686
1104	84-85	0.26306989293664174
1104	86-87	0.2508144255910594
1104	88-89	0.15947158323296406
1104	90-91	0.21340761351341087
1104	92-93	0.20046379091491673
1104	94-95	0.22613769746896395
1104	96-97	0.2535268069233325
1104	98-99	0.3318427591656068
1104	100	0.29886738321133066
1105	1	0.13087900740271508
1105	2	0.13011326910044474
1105	3	0.19795810610316522
1105	4	0.1372882978766654
1105	5	0.1552119814333608
1105	6	0.12829264296333776
1105	7	0.20035108239813582
1105	8	0.16772355252813043
1105	9	0.2179778085211197
1105	10-11	0.29132603902800014
1105	12-13	0.2635099572880435
1105	14-15	0.3949294622630788
1105	16-17	0.383735728610894
1105	18-19	0.3696712716063075
1105	20-21	0.38427330898695544
1105	22-23	0.37744162937066505
1105	24-25	0.4060741579698188
1105	26-27	0.3375632385338321
1105	28-29	0.314268655329343
1105	30-31	0.39240468119599115
1105	32-33	0.37815079161492804
1105	34-35	0.368474373742707
1105	36-37	0.39260821768750276
1105	38-39	0.3706861730580755
1105	40-41	0.3402382580560257
1105	42-43	0.3400274986475722
1105	44-45	0.30834825127674037
1105	46-47	0.28376185660604136
1105	48-49	0.29281584974545183
1105	50-51	0.29504019348367194
1105	52-53	0.3365651422422573
1105	54-55	0.22732915044947077
1105	56-57	0.2954699237931635
1105	58-59	0.2697999424800841
1105	60-61	0.3229590785491041
1105	62-63	0.3041596721137907
1105	64-65	0.3702220187698657
1105	66-67	0.3185911859747179
1105	68-69	0.2573688632881783
1105	70-71	0.29807927709948956
1105	72-73	0.27412876711297685
1105	74-75	0.3107006324662436
1105	76-77	0.29136642767193877
1105	78-79	0.32671581698120633
1105	80-81	0.3365304767281927
1105	82-83	0.29500331556295833
1105	84-85	0.29840543064180736
1105	86-87	0.2631487551459557
1105	88-89	0.2814904234869928
1105	90-91	0.3327359352024004
1105	92-93	0.40374375840526255
1105	94-95	0.39081049075468144
1105	96-97	0.4098586340490016
1105	98-99	0.37302118722349675
1105	100	0.41289287259832363
1106	1	0.10659289398949667
1106	2	0.08388664368557386
1106	3	0.06798743959306464
1106	4	0.06433146875653506
1106	5	0.07852427562048092
1106	6	0.15127148619416886
1106	7	0.1738119018661095
1106	8	0.1358484957362478
1106	9	0.207060191075513
1106	10-11	0.21572529853929723
1106	12-13	0.2576125336356867
1106	14-15	0.3030329207233393
1106	16-17	0.28178854270736053
1106	18-19	0.2776939858574323
1106	20-21	0.32463643105827344
1106	22-23	0.2949647245367686
1106	24-25	0.325411839303392
1106	26-27	0.31688063318259196
1106	28-29	0.26272310934307797
1106	30-31	0.26315206352946774
1106	32-33	0.2875145981065401
1106	34-35	0.29251689390523694
1106	36-37	0.29830969967719767
1106	38-39	0.2604174210789125
1106	40-41	0.20040283452346586
1106	42-43	0.23073174118412254
1106	44-45	0.16515295163771526
1106	46-47	0.20191980013916577
1106	48-49	0.23754310402432566
1106	50-51	0.21330985996840468
1106	52-53	0.2089271854613699
1106	54-55	0.16736950409481466
1106	56-57	0.18107211184213412
1106	58-59	0.13942896392576642
1106	60-61	0.1422707919257391
1106	62-63	0.13109802140919413
1106	64-65	0.2143653751113277
1106	66-67	0.17989486018159795
1106	68-69	0.16327497468398633
1106	70-71	0.1503420114914178
1106	72-73	0.21231962582049135
1106	74-75	0.14178633720420208
1106	76-77	0.11192882776140323
1106	78-79	0.15432943140760358
1106	80-81	0.16967938655631798
1106	82-83	0.1815345830433941
1106	84-85	0.13669483182108166
1106	86-87	0.11109663154348937
1106	88-89	0.07138723286409032
1106	90-91	0.07432240051984706
1106	92-93	0.12984263885940805
1106	94-95	0.13216456340089167
1106	96-97	0.14426721606278647
1106	98-99	0.13033557945005114
1106	100	0.1617350030211817
1107	1	-0.009727371530633633
1107	2	0.054474586463861385
1107	3	0.08133049069230225
1107	4	0.046556510311333454
1107	5	0.04622502864983602
1107	6	0.055677488338197634
1107	7	0.06829793166107123
1107	8	0.10861895762811002
1107	9	0.15863559943345962
1107	10-11	0.12587868097004673
1107	12-13	0.18929066419274676
1107	14-15	0.18533481788740858
1107	16-17	0.16118075565513834
1107	18-19	0.14557766073615142
1107	20-21	0.24415769145984
1107	22-23	0.21086437569408645
1107	24-25	0.26287730780961027
1107	26-27	0.21458737927626714
1107	28-29	0.24791821038154893
1107	30-31	0.24860814719179558
1107	32-33	0.25467914036724437
1107	34-35	0.2014322323355202
1107	36-37	0.22503489369124452
1107	38-39	0.20402545875869293
1107	40-41	0.20294462157989557
1107	42-43	0.23012169941988248
1107	44-45	0.09314696231759001
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2216	48-49	-0.5963489590719533
2216	50-51	-0.6384862791033754
2216	52-53	-0.6211954167469642
2216	54-55	-0.6463151709562709
2216	56-57	-0.584181151622488
2216	58-59	-0.6596954948894975
2216	60-61	-0.6785063723543487
2216	62-63	-0.6653296231697965
2216	64-65	-0.6963340991832325
2216	66-67	-0.6565649170001251
2216	68-69	-0.7310814836674879
2216	70-71	-0.6611970067858834
2216	72-73	-0.6101857494527252
2216	74-75	-0.5534784246472526
2216	76-77	-0.583856378301796
2216	78-79	-0.5836348361785966
2216	80-81	-0.6345995144763137
2216	82-83	-0.5612356949260615
2216	84-85	-0.556712119114593
2216	86-87	-0.6169088644927854
2216	88-89	-0.6343878214701952
2216	90-91	-0.6456772051147439
2216	92-93	-0.5486427380894057
2216	94-95	-0.574877480676804
2216	96-97	-0.5872510625921734
2216	98-99	-0.5754921099448502
2216	100	-0.5441272387807885
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1227.0
1	335.0
2	227.0
3	221.0
4	163.0
5	223.0
6	366.0
7	816.0
8	2206.0
9	3707.0
10	4528.0
11	5555.0
12	7233.0
13	8602.0
14	10090.0
15	11034.0
16	11688.0
17	12777.0
18	13561.0
19	15029.0
20	16507.0
21	18114.0
22	20369.0
23	23047.0
24	27010.0
25	32474.0
26	36563.0
27	37410.0
28	41394.0
29	50995.0
30	60552.0
31	71625.0
32	91931.0
33	121262.0
34	176091.0
35	264586.0
36	467426.0
37	899165.0
38	1101014.0
39	328164.0
40	725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24712938808209	19.47041161761781	13.091864090384634	24.190594903915475
2	14.46397484669828	21.15388467864233	39.944313562870235	24.437826911789156
3	19.047227864364842	25.79432323925495	30.22999070820508	24.928458188175124
4	11.975608479376982	17.462723836682905	39.86521398182032	30.69645370211979
5	12.79753407008675	38.57844360089484	35.73484491009985	12.889177418918555
6	29.79497423245271	38.02423339198607	18.552934619936504	13.627857755624714
7	26.267297380696665	32.65179916804258	23.397299745122872	17.68360370613789
8	25.191281180015835	35.01006031177574	20.950871664085525	18.8477868441229
9	25.54062738070135	16.56101632688429	21.17002909558267	36.72832719683169
10-11	24.285033054729897	26.225664776250685	29.11925599558402	20.370046173435398
12-13	25.538195583545654	24.064024013074363	28.25583159995172	22.141948803428264
14-15	22.674381910584785	25.60758297209869	26.38211695687324	25.335918160443278
16-17	22.139969837638436	28.447104226700336	27.108269052873545	22.30465688278768
18-19	22.072770523923026	28.903688516891773	27.591507893195228	21.432033065989973
20-21	23.130135459683903	27.523359219848388	26.822432364070753	22.524072956396957
22-23	22.667928565158554	27.531104700050125	26.61552287915214	23.18544385563918
24-25	23.039579084034926	27.686274549090168	26.61105595649787	22.663090410377038
26-27	22.95751285137731	27.217159194796864	27.20656580069218	22.61876215313365
28-29	22.41462673345328	28.31750012804565	26.886294080734256	22.381579057766814
30-31	23.01040499780529	27.476658102805164	26.7254093715737	22.78752752781585
32-33	22.71930493579891	27.778886107419808	26.783881605848176	22.717927350933106
34-35	23.108224901121368	27.7015585508897	26.94792561206063	22.2422909359283
36-37	23.11312945049721	27.2429228499914	27.299505484307524	22.344442215203873
38-39	22.88649012859626	27.714009828822196	27.11795588552077	22.28154415706077
40-41	22.617054128427384	27.78002576607872	27.084677273414364	22.518242832079533
42-43	22.70998521005566	27.639123354197565	26.48080106416461	23.170090371582166
44-45	23.015415297021278	27.395450141025414	26.627384191151837	22.96175037080147
46-47	23.067968761838635	27.58761859927712	27.004410908134112	22.340001730750135
48-49	23.03361104570481	27.36160304999324	27.166820301893218	22.43796560240873
50-51	22.02807476149528	27.655501169886097	27.634272640445822	22.6821514281728
52-53	22.390256610655204	27.99433372411098	26.539714030921534	23.075695634312282
54-55	22.23876630487601	27.48300255187152	26.767774681867685	23.51045646138478
56-57	23.03929396584731	26.92150988645955	26.767768805477516	23.271427342215624
58-59	23.347337835666227	26.952611023663863	26.98964824162144	22.710402899048468
60-61	22.906453486012712	27.322843640076535	27.434166787325193	22.33653608658556
62-63	22.253699514246573	27.36334161083963	27.319666137517494	23.063292737396303
64-65	21.92747922844316	28.090995789699935	26.927119748429124	23.054405233427783
66-67	22.786226802966695	27.14034734361746	26.58449284054089	23.488933012874952
68-69	22.46732849912316	27.13333123742061	26.955157780268102	23.44418248318813
70-71	22.85219345299046	27.093263791163057	26.725562070958812	23.328980684887668
72-73	22.93196684002514	26.561998596665614	27.6129337228868	22.89310084042244
74-75	22.51957931021957	27.718654158454477	26.952591921769187	22.80917460955677
76-77	22.488313776967246	26.93896109101476	27.0947147709851	23.478010361032897
78-79	22.67819096665805	26.99536917747879	27.273238570721915	23.05320128514125
80-81	22.7956862105061	26.93470507754202	27.249362349624672	23.020246362327203
82-83	22.58531461830371	26.61950121786316	27.798219896796354	22.996964267036777
84-85	22.541493002821415	27.131278907005946	27.149591505058822	23.177636585113813
86-87	22.412423523470338	27.200117938501133	27.13120357643244	23.25625496159609
88-89	22.544342167510305	26.805741920687925	26.909075150061767	23.74084076174
90-91	22.826429902950707	26.441927178022816	27.0492383340884	23.682404584938077
92-93	23.07150091848647	26.843025420664553	27.15622636386192	22.92924729698706
94-95	22.41940752229857	26.718407768780196	27.487773609633226	23.374411099288007
96-97	22.205432693499912	26.648267625393984	27.811850849536235	23.33444883156987
98-99	22.60550349774383	26.946493688428735	26.96971678345854	23.478286030368896
100	22.66461893070352	26.189813784638016	26.83754993175189	24.30801735290657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1649.0
1	1082.5
2	520.5
3	542.0
4	508.5
5	427.5
6	390.0
7	385.0
8	406.0
9	434.0
10	405.0
11	365.0
12	383.0
13	420.5
14	461.5
15	540.0
16	663.5
17	831.0
18	1026.5
19	1297.5
20	1853.5
21	2631.5
22	4019.5
23	6372.5
24	9043.0
25	12400.0
26	17174.0
27	23017.5
28	29589.5
29	36480.5
30	44718.5
31	53154.0
32	61817.0
33	70026.0
34	77583.0
35	85826.5
36	95029.0
37	105407.5
38	111904.5
39	114725.5
40	117161.0
41	119112.5
42	123352.0
43	128597.5
44	133301.5
45	140754.0
46	149626.0
47	170583.5
48	208162.0
49	213755.0
50	197034.5
51	179937.5
52	154422.5
53	139868.0
54	129284.5
55	116295.0
56	104470.5
57	93001.0
58	81533.0
59	71867.0
60	60590.5
61	47717.0
62	36192.5
63	27412.0
64	20089.5
65	14384.0
66	10677.0
67	7841.5
68	5581.5
69	4018.0
70	3141.5
71	2487.0
72	1913.5
73	1663.0
74	1397.5
75	1092.0
76	845.0
77	573.5
78	405.0
79	290.0
80	195.5
81	134.5
82	96.5
83	72.5
84	54.0
85	51.5
86	48.5
87	35.5
88	30.0
89	27.0
90	23.0
91	22.5
92	20.5
93	17.5
94	11.0
95	7.5
96	7.5
97	7.5
98	8.0
99	9.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10024994919935176
2	0.06456437067756554
3	0.08113088749483235
4	0.08290765893595915
5	0.08408383158008535
6	0.08976449520171612
7	0.0875873245625889
8	0.08986459500121621
9	0.07422400132932533
10-11	0.08108083759508229
12-13	0.07142120694332249
14-15	0.08068043839708189
16-17	0.082269522714146
18-19	0.08180656114145803
20-21	0.07362340253232473
22-23	0.0772395077892659
24-25	0.07997973980058118
26-27	0.07401128925538762
28-29	0.08246972231314621
30-31	0.08657381409265037
32-33	0.08802526118540185
34-35	0.09052775617290439
36-37	0.09061534349746697
38-39	0.09189161594109327
40-41	0.08892615938090276
42-43	0.09440662340353333
44-45	0.09125347971928012
46-47	0.088175410884652
48-49	0.09689660591609836
50-51	0.09366838738222007
52-53	0.09939910090360089
54-55	0.09129101714409266
56-57	0.09786006648628684
58-59	0.10213933291491617
60-61	0.10085054799635236
62-63	0.10182652104147834
64-65	0.10347816773323003
66-67	0.10585553797135745
68-69	0.10299018121066703
70-71	0.10484202750141891
72-73	0.10237706993872892
74-75	0.10596815024579505
76-77	0.10340309288360496
78-79	0.10982199252654898
80-81	0.1053175015490444
82-83	0.109847017476424
84-85	0.10571790074704479
86-87	0.10262731943747917
88-89	0.10191410836604094
90-91	0.10188908341616591
92-93	0.0962584696942852
94-95	0.09579550812159723
96-97	0.09446918577822089
98-99	0.10750718466310911
100	0.24577003272262446
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3996012.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.523149554403744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99142114463348	46.2617668996879
2	14.312642825781932	17.89743015830255
3	5.346520484761073	10.028438995931992
4	2.5039160552935305	6.262108719871604
5	1.3327605469665147	4.166419349909817
6	0.7848172536812952	2.944154791487523
7	0.49645222001287864	2.1727829478936678
8	0.29824499167517765	1.4917772974687225
9	0.21859136541721602	1.2300318568153725
>10	0.7044462579249706	6.547760574541906
>50	0.008514104163651552	0.3510619814559907
>100	0.0014880530027009742	0.1273844489310728
>500	6.156556186067588E-5	0.037953830119733106
>1k	6.156556186067588E-5	0.08703205872283624
>5k	0.0	0.0
>10k+	6.156556186067588E-5	0.3938960888592584
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	10233	0.2560803120711349	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.25124749375127E-4
2	0.0	0.0	0.0	5.00498997500508E-5	1.25124749375127E-4
3	0.0	0.0	0.0	5.00498997500508E-5	1.25124749375127E-4
4	0.0	0.0	0.0	5.00498997500508E-5	1.25124749375127E-4
5	0.0	0.0	0.0	7.50748496250762E-5	1.25124749375127E-4
6	0.0	0.0	0.0	1.000997995001016E-4	2.001995990002032E-4
7	0.0	0.0	0.0	1.25124749375127E-4	2.001995990002032E-4
8	0.0	0.0	0.0	1.25124749375127E-4	2.001995990002032E-4
9	0.0	0.0	0.0	1.751746491251778E-4	2.001995990002032E-4
10-11	0.0	0.0	0.0	2.001995990002032E-4	2.001995990002032E-4
12-13	0.0	0.0	0.0	2.001995990002032E-4	4.129116729379191E-4
14-15	0.0	0.0	0.0	2.50249498750254E-4	5.630613721880715E-4
16-17	0.0	0.0	0.0	2.877869235627921E-4	7.257235463757366E-4
18-19	0.0	2.50249498750254E-5	0.0	3.002993985003048E-4	8.383358208133509E-4
20-21	0.0	2.50249498750254E-5	0.0	3.878867230628937E-4	9.259231453759397E-4
22-23	0.0	2.50249498750254E-5	0.0	5.505488972505588E-4	9.509480952509652E-4
24-25	0.0	2.50249498750254E-5	0.0	0.0011511476942511684	9.509480952509652E-4
26-27	0.0	2.50249498750254E-5	0.0	0.00217717063912721	9.759730451259906E-4
28-29	0.0	2.50249498750254E-5	0.0	0.005543026397318126	9.884855200635034E-4
30-31	0.0	2.50249498750254E-5	0.0	0.01534029427339057	0.001000997995001016
32-33	0.0	2.50249498750254E-5	0.0	0.03607346524484911	0.0010385354198135543
34-35	0.0	2.50249498750254E-5	0.0	0.07055784617263411	0.0010760728446260921
36-37	2.50249498750254E-5	2.50249498750254E-5	0.0	0.12024488414949705	0.0010760728446260921
38-39	2.50249498750254E-5	2.50249498750254E-5	0.0	0.19470662250263512	0.0011386352193136556
40-41	2.50249498750254E-5	2.50249498750254E-5	0.0	0.284733879678039	0.0011511476942511684
42-43	2.50249498750254E-5	2.50249498750254E-5	0.0	0.3700689587518756	0.0011511476942511684
44-45	2.50249498750254E-5	2.50249498750254E-5	0.0	0.47801408003779766	0.0011886851190637065
46-47	2.50249498750254E-5	2.50249498750254E-5	0.0	0.6094326043064936	0.0012387350188137573
48-49	2.50249498750254E-5	2.50249498750254E-5	0.0	0.7448175831303809	0.00125124749375127
50-51	2.50249498750254E-5	2.50249498750254E-5	0.0	0.892151975519593	0.0012762724436262954
52-53	2.50249498750254E-5	2.50249498750254E-5	0.0	1.0448166822321854	0.0013763722431263971
54-55	2.50249498750254E-5	2.50249498750254E-5	0.0	1.2131595200414813	0.0014139096679389352
56-57	2.50249498750254E-5	2.50249498750254E-5	0.0	1.4192399822623156	0.0014514470927514732
58-59	2.50249498750254E-5	2.50249498750254E-5	0.0	1.6459535156551082	0.0014764720426264986
60-61	2.50249498750254E-5	2.50249498750254E-5	0.0	1.8775218893236556	0.0014764720426264986
62-63	2.50249498750254E-5	2.50249498750254E-5	0.0	2.1185121566201506	0.0014764720426264986
64-65	2.50249498750254E-5	2.50249498750254E-5	0.0	2.3710889757087816	0.0014764720426264986
66-67	2.50249498750254E-5	2.50249498750254E-5	0.0	2.6591011238204487	0.001501496992501524
68-69	2.50249498750254E-5	2.50249498750254E-5	0.0	2.9910445714377234	0.0015265219423765495
70-71	2.50249498750254E-5	2.50249498750254E-5	0.0	3.3582982233286587	0.0015515468922515747
72-73	2.50249498750254E-5	3.7537424812538096E-5	0.0	3.717994340357336	0.001626621741876651
74-75	2.50249498750254E-5	5.00498997500508E-5	0.0	4.080067827624141	0.00171420906643924
76-77	2.50249498750254E-5	5.00498997500508E-5	0.0	4.475587160398918	0.0017767714411268034
78-79	2.50249498750254E-5	5.00498997500508E-5	0.0	4.9299651752797535	0.0018017963910018288
80-81	2.50249498750254E-5	5.00498997500508E-5	0.0	5.417764010718686	0.0018017963910018288
82-83	2.50249498750254E-5	5.00498997500508E-5	0.0	5.912795056671501	0.0018768712406269051
84-85	2.50249498750254E-5	5.00498997500508E-5	0.0	6.404447734391187	0.0019018961905019303
86-87	2.50249498750254E-5	5.00498997500508E-5	0.0	6.910026796716327	0.0019519460902519812
88	2.50249498750254E-5	5.00498997500508E-5	0.0	7.310313382442295	0.002001995990002032
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1330	0.0	19.804317	1
TCTAACG	590	0.0	19.12531	2
TGATCGC	770	0.0	18.307522	9
CATGGGG	6865	0.0	18.28514	4
CTAGTAC	1985	0.0	18.237867	3
ATACGGG	830	0.0	17.560118	3
GTATATA	2680	0.0	17.550467	1
TAGTACT	2420	0.0	17.484558	4
TACGGGC	840	0.0	17.350418	4
CTGTGCG	1880	0.0	17.246075	9
CTAACGC	655	0.0	17.227163	3
GTATAAG	2070	0.0	16.58731	1
TACCTGG	3735	0.0	16.364473	2
GGGCTAT	2170	0.0	16.24217	6
GTGATCG	840	0.0	16.223108	8
CTATTGA	2150	0.0	16.173061	9
GGCGTCG	875	0.0	16.111225	8
GTACATG	22075	0.0	16.086803	1
TAACGCC	740	0.0	15.88312	4
TACATGG	22110	0.0	15.820984	2
>>END_MODULE