FastQCFastQC Report
Mon 16 Jan 2017
ERR1146619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996012
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT235500.5893375695568481No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA98930.24757182911362627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77940.19504445932594797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75780.18963907015294248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43200.10810778346010974No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42540.10645613676835805No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT98550.020.84043112-13
TCTAACG5300.019.5170212
GTACTTT109650.018.6664414-15
CTAACGC5950.018.1749043
AGTACTT108650.018.08125112-13
TAACGCC6400.017.6311914
GTACTAG13600.017.2867811
CATGGGG70450.017.0180424
CTAGTAC19250.016.8530923
TAGGACT25550.016.7456074
GTATAAG21450.016.4405751
TATACCG5150.016.4274045
CGCCTAC6050.016.3132467
GGCGTCG9700.015.9889038
ACTTTTT129000.015.88458816-17
GTCGACG5050.015.82139936-37
ACTACGC2104.329564E-415.6725643
CGATAAT7950.015.66667966-67
CGACGAG5850.015.66589524-25
GTACATG217800.015.6517581
Produced by FastQC (version 0.11.5)