##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146619_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3996012 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.271761446161825 33.0 31.0 34.0 30.0 34.0 2 32.375347471429016 34.0 31.0 34.0 30.0 34.0 3 32.531846751210956 34.0 31.0 34.0 31.0 34.0 4 36.036486877416785 37.0 35.0 37.0 35.0 37.0 5 36.056596927136354 37.0 35.0 37.0 35.0 37.0 6 36.134761106823504 37.0 36.0 37.0 35.0 37.0 7 36.116755154889425 37.0 36.0 37.0 35.0 37.0 8 36.12177841307784 37.0 36.0 37.0 35.0 37.0 9 37.91666316317369 39.0 38.0 39.0 35.0 39.0 10-11 37.86308724798624 39.0 38.0 39.0 35.0 39.0 12-13 37.837523010441416 39.0 38.0 39.0 35.0 39.0 14-15 39.204568955248384 40.0 39.0 41.0 36.0 41.0 16-17 39.15150429978689 40.0 39.0 41.0 36.0 41.0 18-19 39.24425827550067 40.5 39.0 41.0 36.0 41.0 20-21 39.29829152164708 41.0 39.0 41.0 36.0 41.0 22-23 39.268377071940726 41.0 39.0 41.0 36.0 41.0 24-25 39.26083730479287 41.0 39.0 41.0 36.0 41.0 26-27 39.18688056992822 41.0 39.0 41.0 36.0 41.0 28-29 39.13892888710044 40.0 39.0 41.0 36.0 41.0 30-31 39.037224988313355 40.0 39.0 41.0 35.0 41.0 32-33 38.95106683863813 40.0 38.0 41.0 35.0 41.0 34-35 38.891122574206484 40.0 38.0 41.0 35.0 41.0 36-37 38.83847258216442 40.0 38.0 41.0 35.0 41.0 38-39 38.76897341649625 40.0 38.0 41.0 35.0 41.0 40-41 38.71412523285716 40.0 38.0 41.0 35.0 41.0 42-43 38.63725534357755 40.0 38.0 41.0 35.0 41.0 44-45 38.53277129798409 40.0 38.0 41.0 34.5 41.0 46-47 38.344813904462754 40.0 38.0 41.0 34.0 41.0 48-49 38.10871689074007 40.0 38.0 41.0 33.0 41.0 50-51 38.01398481786341 40.0 38.0 41.0 33.0 41.0 52-53 37.95041068445239 40.0 37.0 41.0 33.0 41.0 54-55 37.80511044511378 40.0 37.0 41.0 33.0 41.0 56-57 37.65278257422651 40.0 37.0 41.0 33.0 41.0 58-59 37.490475754327065 40.0 36.0 41.0 33.0 41.0 60-61 37.2666410911679 39.0 36.0 41.0 32.5 41.0 62-63 37.03728217032381 39.0 35.0 41.0 32.0 41.0 64-65 36.77895887199538 39.0 35.0 41.0 32.0 41.0 66-67 36.494970485574115 38.0 35.0 40.0 32.0 41.0 68-69 36.18729736046839 37.0 35.0 40.0 31.0 41.0 70-71 35.84205202586979 37.0 35.0 39.0 31.0 41.0 72-73 35.491124901526824 36.0 35.0 39.0 31.0 41.0 74-75 35.16597222430764 36.0 35.0 39.0 31.0 40.5 76-77 34.845535749141895 35.0 35.0 37.5 31.0 39.5 78-79 34.543451821466 35.0 34.0 37.0 30.5 39.0 80-81 34.26163910418687 35.0 34.0 36.5 30.0 39.0 82-83 33.99693081502258 35.0 34.0 36.0 30.0 38.0 84-85 33.78220097937644 35.0 34.0 36.0 30.0 37.0 86-87 33.60421690425354 35.0 34.0 35.5 30.0 37.0 88-89 33.39657163191702 35.0 34.0 35.0 29.0 36.0 90-91 33.300569292584704 35.0 34.0 35.0 29.0 36.0 92-93 33.18645602165358 35.0 34.0 35.0 29.0 36.0 94-95 33.11008850824272 35.0 34.0 35.0 29.0 36.0 96-97 33.05380051411257 35.0 34.0 35.0 29.0 35.5 98-99 32.99807883459809 35.0 34.0 35.0 29.0 35.0 100 32.23826454975611 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28255922296670377 1101 2 0.31684656206652306 1101 3 0.271716033047511 1101 4 0.19083996012773952 1101 5 0.18885534034439644 1101 6 0.23071758760940497 1101 7 0.21457377890713758 1101 8 0.22235215594907487 1101 9 0.2622425858075772 1101 10-11 0.27281681631779975 1101 12-13 0.2676640912067825 1101 14-15 0.42460282797625837 1101 16-17 0.4609320470104237 1101 18-19 0.44827403041491465 1101 20-21 0.4110413252693874 1101 22-23 0.3910654916011822 1101 24-25 0.39390267186516326 1101 26-27 0.3749777474692877 1101 28-29 0.4223354583963612 1101 30-31 0.5064930848912255 1101 32-33 0.45191422407881277 1101 34-35 0.4611450424387371 1101 36-37 0.45227994183849773 1101 38-39 0.466307549674319 1101 40-41 0.45634765765699825 1101 42-43 0.48601805265386133 1101 44-45 0.44952751707100447 1101 46-47 0.5236965351051381 1101 48-49 0.6175632900971308 1101 50-51 0.577199083271168 1101 52-53 0.5235782189424754 1101 54-55 0.5089764081540409 1101 56-57 0.47007856344954746 1101 58-59 0.4933060211394036 1101 60-61 0.5288348752408965 1101 62-63 0.4636812753675912 1101 64-65 0.4244459689853386 1101 66-67 0.39317179378137723 1101 68-69 0.31649731890114907 1101 70-71 0.35565658190540006 1101 72-73 0.39722668984525455 1101 74-75 0.4057290852905169 1101 76-77 0.3728898710702282 1101 78-79 0.31903448825980263 1101 80-81 0.30440612757151797 1101 82-83 0.3395543443342248 1101 84-85 0.3579624780309629 1101 86-87 0.3274550980255029 1101 88-89 0.424941682605251 1101 90-91 0.3455783514041073 1101 92-93 0.3801107421782106 1101 94-95 0.3966811717761587 1101 96-97 0.308541757737963 1101 98-99 0.3500316848149012 1101 100 0.3745245607775729 1102 1 0.2475356275034386 1102 2 0.23281673259469216 1102 3 0.27530541779845663 1102 4 0.2256332553879119 1102 5 0.19699889688633476 1102 6 0.22379120592598412 1102 7 0.23348283918807056 1102 8 0.24844028023343157 1102 9 0.316692091751797 1102 10-11 0.319294460117014 1102 12-13 0.32975974059445434 1102 14-15 0.4522011561499326 1102 16-17 0.507417347849632 1102 18-19 0.4489904054072653 1102 20-21 0.35570669495128016 1102 22-23 0.4249259532382297 1102 24-25 0.4614059585023327 1102 26-27 0.4591399553826676 1102 28-29 0.4121610192450902 1102 30-31 0.40434861303850056 1102 32-33 0.39372278158671037 1102 34-35 0.40888736419462646 1102 36-37 0.3877692325845459 1102 38-39 0.3911772621937004 1102 40-41 0.3900403417675804 1102 42-43 0.40005850185117 1102 44-45 0.4441491827245869 1102 46-47 0.43563527407310687 1102 48-49 0.49943666150362276 1102 50-51 0.4557878790190344 1102 52-53 0.38263184413861495 1102 54-55 0.4010301087425745 1102 56-57 0.4036960024170497 1102 58-59 0.302484485537704 1102 60-61 0.2631273411847417 1102 62-63 0.2715341883412066 1102 64-65 0.38733126534812357 1102 66-67 0.34624326812675577 1102 68-69 0.3156581367672615 1102 70-71 0.3113195225385965 1102 72-73 0.34011680425321345 1102 74-75 0.3142412988583203 1102 76-77 0.2942812259186667 1102 78-79 0.2757150789592089 1102 80-81 0.2743002660484919 1102 82-83 0.3328575266000442 1102 84-85 0.2661717967664501 1102 86-87 0.3089658496876737 1102 88-89 0.3192676098138918 1102 90-91 0.27824882067463363 1102 92-93 0.2727297384698488 1102 94-95 0.2506129734658913 1102 96-97 0.3094031747380086 1102 98-99 0.3126875644792193 1102 100 0.31938592123331944 1103 1 0.23298175884223582 1103 2 0.25504807204864477 1103 3 0.241351598802531 1103 4 0.19855487068790723 1103 5 0.1677793851478171 1103 6 0.1765383650387946 1103 7 0.1992130372215044 1103 8 0.18160895278284528 1103 9 0.2441497386085132 1103 10-11 0.23729698247464626 1103 12-13 0.21674658314890394 1103 14-15 0.3537970663075214 1103 16-17 0.4289074522348244 1103 18-19 0.3648737908285753 1103 20-21 0.3338766490521863 1103 22-23 0.3877443382575976 1103 24-25 0.3014879475143033 1103 26-27 0.38107189219653037 1103 28-29 0.36682940437458456 1103 30-31 0.41702155366370874 1103 32-33 0.3870976008480298 1103 34-35 0.3708533316379885 1103 36-37 0.3251047025711955 1103 38-39 0.3732639666544415 1103 40-41 0.3670111642400755 1103 42-43 0.39635738526329334 1103 44-45 0.41373583900669075 1103 46-47 0.4237792709059818 1103 48-49 0.47787142598997434 1103 50-51 0.4227568872220644 1103 52-53 0.35704878638702553 1103 54-55 0.3566242117886773 1103 56-57 0.3767876538592745 1103 58-59 0.3684503527695213 1103 60-61 0.3572836590821922 1103 62-63 0.3745765379055044 1103 64-65 0.38381914663378325 1103 66-67 0.3193291725469649 1103 68-69 0.33628757126579245 1103 70-71 0.3449990330767747 1103 72-73 0.3269115586192939 1103 74-75 0.3134199220838312 1103 76-77 0.24010036720483185 1103 78-79 0.2592385389225029 1103 80-81 0.2695977910986258 1103 82-83 0.24870691167557624 1103 84-85 0.28880924763800664 1103 86-87 0.32501838338793476 1103 88-89 0.33254429746992287 1103 90-91 0.3282482562964333 1103 92-93 0.338870631773176 1103 94-95 0.2998823308125509 1103 96-97 0.29094862673061783 1103 98-99 0.3030917928007568 1103 100 0.32183415260966086 1104 1 0.20138243863438277 1104 2 0.1815593467892782 1104 3 0.20736140462508956 1104 4 0.19012877201375744 1104 5 0.16830855412960943 1104 6 0.20449799464005736 1104 7 0.18758474600584663 1104 8 0.20230324673266153 1104 9 0.23733751082274068 1104 10-11 0.22617633566186868 1104 12-13 0.26719584559396736 1104 14-15 0.3749505784311893 1104 16-17 0.404908491801919 1104 18-19 0.30495099253555225 1104 20-21 0.30051149866528704 1104 22-23 0.329595321691329 1104 24-25 0.3238642382150658 1104 26-27 0.329819881905145 1104 28-29 0.321145514242815 1104 30-31 0.3562110986820173 1104 32-33 0.33637852348827124 1104 34-35 0.328057922679136 1104 36-37 0.32487871893378184 1104 38-39 0.28945825490129806 1104 40-41 0.29717426873286 1104 42-43 0.3481988529104356 1104 44-45 0.33100744790786507 1104 46-47 0.34031752876750687 1104 48-49 0.4032518537767231 1104 50-51 0.3343058747847323 1104 52-53 0.32021320907696804 1104 54-55 0.2861389066724769 1104 56-57 0.26871016863399433 1104 58-59 0.29171115538701287 1104 60-61 0.270058651464673 1104 62-63 0.2732857312307715 1104 64-65 0.24695811785341704 1104 66-67 0.2546604571618616 1104 68-69 0.2631802595895678 1104 70-71 0.26747781401327586 1104 72-73 0.20521745531108593 1104 74-75 0.26479404077371527 1104 76-77 0.27055103094723876 1104 78-79 0.31038968839366987 1104 80-81 0.28568839963643455 1104 82-83 0.22316940823343856 1104 84-85 0.21648118763641122 1104 86-87 0.2860410534459561 1104 88-89 0.300578391360105 1104 90-91 0.18687346954618533 1104 92-93 0.17960758382110953 1104 94-95 0.2900332309831555 1104 96-97 0.2771427031534657 1104 98-99 0.2496854507897055 1104 100 0.40218688847978257 1105 1 0.15068560292324662 1105 2 0.20690510248610394 1105 3 0.1832044437455096 1105 4 0.13630029803361765 1105 5 0.1195499265820601 1105 6 0.14235804182290934 1105 7 0.14112769563075034 1105 8 0.14398405546517523 1105 9 0.19005644401743638 1105 10-11 0.19272032900960312 1105 12-13 0.21002940038237483 1105 14-15 0.31375338998402214 1105 16-17 0.3831305769142688 1105 18-19 0.24686643141199482 1105 20-21 0.24335300861039855 1105 22-23 0.2981004804068803 1105 24-25 0.2610142563412339 1105 26-27 0.2602936600330068 1105 28-29 0.24872810967532644 1105 30-31 0.27974984366208133 1105 32-33 0.29207849506581596 1105 34-35 0.19887239448726746 1105 36-37 0.2696239542568719 1105 38-39 0.2656178246964416 1105 40-41 0.2872484724929265 1105 42-43 0.2966985463706848 1105 44-45 0.303328544967826 1105 46-47 0.2891800274853651 1105 48-49 0.37533880614522275 1105 50-51 0.33292607790744455 1105 52-53 0.3060092141162727 1105 54-55 0.31687592532090036 1105 56-57 0.2994345968375356 1105 58-59 0.28048569324799644 1105 60-61 0.2996528053640688 1105 62-63 0.29491912221810423 1105 64-65 0.3013079521340387 1105 66-67 0.27171203805350785 1105 68-69 0.23778005491654142 1105 70-71 0.2638315804589553 1105 72-73 0.28673626385410955 1105 74-75 0.23967818597411394 1105 76-77 0.24007661076932862 1105 78-79 0.2669282556354915 1105 80-81 0.2924830430598533 1105 82-83 0.2503794893588136 1105 84-85 0.18621316305999613 1105 86-87 0.25995041007265485 1105 88-89 0.2833448597481407 1105 90-91 0.29101494292577 1105 92-93 0.3422113629903478 1105 94-95 0.2843723226702508 1105 96-97 0.2636618914456079 1105 98-99 0.2972666525038221 1105 100 0.32486238933754663 1106 1 0.11750872439672122 1106 2 0.08422731725450205 1106 3 0.11016832929023934 1106 4 0.08529919488670146 1106 5 0.07700225385525528 1106 6 0.14086868034883082 1106 7 0.1330096275895869 1106 8 0.12774488254311223 1106 9 0.13976101905816307 1106 10-11 0.119238186163102 1106 12-13 0.14089340688858698 1106 14-15 0.19049477938225579 1106 16-17 0.24139005748128994 1106 18-19 0.20874291313671023 1106 20-21 0.18858482487755168 1106 22-23 0.18600664729945038 1106 24-25 0.19973483517003388 1106 26-27 0.18816054391508885 1106 28-29 0.20556733771304891 1106 30-31 0.18814172746378688 1106 32-33 0.16593810220355465 1106 34-35 0.12952276527348516 1106 36-37 0.1122402863570855 1106 38-39 0.1726021481725084 1106 40-41 0.12362947297560112 1106 42-43 0.2064712990586699 1106 44-45 0.20651110238463843 1106 46-47 0.2808083684407663 1106 48-49 0.31876819849026106 1106 50-51 0.2649205182280099 1106 52-53 0.24094105254639686 1106 54-55 0.27936300535424863 1106 56-57 0.2621707572741272 1106 58-59 0.24500711350767546 1106 60-61 0.26406918669682256 1106 62-63 0.24777278096092914 1106 64-65 0.24118650474525793 1106 66-67 0.1524688143445303 1106 68-69 0.23450242655260212 1106 70-71 0.22935614033453078 1106 72-73 0.1352027252939294 1106 74-75 0.1405805084657743 1106 76-77 0.059469931770308904 1106 78-79 0.10215031471524583 1106 80-81 0.0883505621773466 1106 82-83 0.14765222269438283 1106 84-85 0.1538834883841318 1106 86-87 0.1702537760700764 1106 88-89 0.10581838475806649 1106 90-91 0.11589741127386333 1106 92-93 0.1465990634560086 1106 94-95 0.15871719893213054 1106 96-97 0.15074022178398394 1106 98-99 0.16735639528587853 1106 100 0.1461998683342216 1107 1 0.0603644698639485 1107 2 -0.0019412940184011518 1107 3 0.04049351562866832 1107 4 0.027488121267019494 1107 5 0.030358615594835214 1107 6 0.05766933876685698 1107 7 0.061960808569594406 1107 8 0.04481992081003483 1107 9 0.050397432879606185 1107 10-11 0.0856058860502742 1107 12-13 0.04422929837036804 1107 14-15 0.11283460531895884 1107 16-17 0.18913102207681476 1107 18-19 0.1604517379648911 1107 20-21 0.10841278812726785 1107 22-23 0.11451829399274516 1107 24-25 0.0989658520041985 1107 26-27 0.09910348507518307 1107 28-29 0.08139896021879878 1107 30-31 0.11702217811601656 1107 32-33 0.12283498779921587 1107 34-35 0.08085602902706057 1107 36-37 0.052144693353788796 1107 38-39 0.08394887818484875 1107 40-41 0.10151632925649068 1107 42-43 0.1389173421736345 1107 44-45 0.09581612104364012 1107 46-47 0.12653911744744306 1107 48-49 0.2005853091686589 1107 50-51 0.13957514478708788 1107 52-53 0.12346903519576102 1107 54-55 0.16341037372973233 1107 56-57 0.1248883393242366 1107 58-59 0.1267384877961888 1107 60-61 0.04959543905654584 1107 62-63 0.08657163607736607 1107 64-65 0.12734443334003487 1107 66-67 0.09067803360106552 1107 68-69 0.06343919820280775 1107 70-71 0.05188944794207373 1107 72-73 0.03667221952001398 1107 74-75 0.04967150265220255 1107 76-77 0.05994997949953529 1107 78-79 0.12107234473766937 1107 80-81 0.09550792726962953 1107 82-83 0.04190453234087954 1107 84-85 0.05076806023338776 1107 86-87 0.0779537989799195 1107 88-89 0.16728333211808888 1107 90-91 0.12010235558703641 1107 92-93 0.12614094505354956 1107 94-95 0.12216620511544818 1107 96-97 0.15242897417588352 1107 98-99 0.12174937462513924 1107 100 0.10665266411862717 1108 1 -0.008653024694638134 1108 2 0.007091996220466967 1108 3 0.0348604294020447 1108 4 0.045352792034883294 1108 5 0.02423947206801813 1108 6 0.1023233397801917 1108 7 0.016649516360246253 1108 8 0.035369962351701645 1108 9 0.08295790442054596 1108 10-11 0.08023960709660116 1108 12-13 0.07442250371371273 1108 14-15 0.15549106295605242 1108 16-17 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78-79 -0.31057303551241233 2214 80-81 -0.33068853381895025 2214 82-83 -0.3131353979906564 2214 84-85 -0.29578816027897403 2214 86-87 -0.2652329809372205 2214 88-89 -0.22335325743011936 2214 90-91 -0.2879835593165154 2214 92-93 -0.2709487703170055 2214 94-95 -0.21781886779747595 2214 96-97 -0.2306871098912069 2214 98-99 -0.21497858210817355 2214 100 -0.42659795473391426 2215 1 -0.37274158150454895 2215 2 -0.4054848186558786 2215 3 -0.426750188007631 2215 4 -0.3476065539692854 2215 5 -0.3078309525939886 2215 6 -0.30220329909520416 2215 7 -0.3204905838010532 2215 8 -0.2840102450779298 2215 9 -0.3922788297546589 2215 10-11 -0.40071073335859353 2215 12-13 -0.42793894505035013 2215 14-15 -0.4779681329320127 2215 16-17 -0.4685549585836526 2215 18-19 -0.5136172950178235 2215 20-21 -0.5237082770022994 2215 22-23 -0.4486345553082245 2215 24-25 -0.5063261575532039 2215 26-27 -0.49633189299765945 2215 28-29 -0.4621512800978991 2215 30-31 -0.44421239249126643 2215 32-33 -0.434688967430084 2215 34-35 -0.48273186127345014 2215 36-37 -0.5840821503543481 2215 38-39 -0.5487757646003288 2215 40-41 -0.590105382022351 2215 42-43 -0.5963861002474289 2215 44-45 -0.6477831008143369 2215 46-47 -0.5338280005167988 2215 48-49 -0.4723081806379099 2215 50-51 -0.5139091848035662 2215 52-53 -0.5285131308631321 2215 54-55 -0.5902072471736801 2215 56-57 -0.5624895636966372 2215 58-59 -0.5915571194202016 2215 60-61 -0.55754969089719 2215 62-63 -0.5455255300017896 2215 64-65 -0.4936458908139727 2215 66-67 -0.45694285046671723 2215 68-69 -0.44267182122109006 2215 70-71 -0.45598181252423586 2215 72-73 -0.4483286857889084 2215 74-75 -0.3980618957504021 2215 76-77 -0.4748847284052573 2215 78-79 -0.42953861445509744 2215 80-81 -0.3765879052517178 2215 82-83 -0.4558368692504686 2215 84-85 -0.40874075487840145 2215 86-87 -0.4346411847797782 2215 88-89 -0.3675818597392464 2215 90-91 -0.39093196031807054 2215 92-93 -0.3868440445008545 2215 94-95 -0.3811951802079818 2215 96-97 -0.36541815976158176 2215 98-99 -0.35875314458343865 2215 100 -0.5234437277970656 2216 1 -0.41577026405055406 2216 2 -0.4776813277259393 2216 3 -0.46399444839435233 2216 4 -0.3325566712805781 2216 5 -0.30043670083288987 2216 6 -0.3336436831884768 2216 7 -0.36434585248124307 2216 8 -0.32556016309877833 2216 9 -0.4131676347873281 2216 10-11 -0.46172107952487806 2216 12-13 -0.4610963710509637 2216 14-15 -0.5220680623821679 2216 16-17 -0.4488850051219444 2216 18-19 -0.5024359241250451 2216 20-21 -0.540287866153875 2216 22-23 -0.5409017586991922 2216 24-25 -0.5222675152114604 2216 26-27 -0.5460878973874372 2216 28-29 -0.5706379029488957 2216 30-31 -0.5200950666107715 2216 32-33 -0.5912732191368306 2216 34-35 -0.6359364041856921 2216 36-37 -0.5924576915297308 2216 38-39 -0.5667843564614614 2216 40-41 -0.5814961051468188 2216 42-43 -0.5849634659059078 2216 44-45 -0.5689011821160221 2216 46-47 -0.5223009074879883 2216 48-49 -0.5527290143276673 2216 50-51 -0.5712105681069346 2216 52-53 -0.5573158705201848 2216 54-55 -0.5667832358799814 2216 56-57 -0.6633347528466089 2216 58-59 -0.7238581236540469 2216 60-61 -0.6676897184648425 2216 62-63 -0.6630314863999232 2216 64-65 -0.5716181856951579 2216 66-67 -0.5877681656451443 2216 68-69 -0.5659043559145331 2216 70-71 -0.5840387579219168 2216 72-73 -0.5092132059054819 2216 74-75 -0.5008555559055594 2216 76-77 -0.496257830518573 2216 78-79 -0.5238870525885204 2216 80-81 -0.5487792182083737 2216 82-83 -0.5444201784330218 2216 84-85 -0.5007507095302444 2216 86-87 -0.4196321414682913 2216 88-89 -0.3487519922934297 2216 90-91 -0.39076184392799007 2216 92-93 -0.4205661589306402 2216 94-95 -0.4231202340143909 2216 96-97 -0.44239725672355945 2216 98-99 -0.4597607972104214 2216 100 -0.5899134261235233 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 25.0 8 125.0 9 235.0 10 242.0 11 161.0 12 157.0 13 230.0 14 427.0 15 705.0 16 1257.0 17 2253.0 18 3954.0 19 5749.0 20 8018.0 21 11966.0 22 17170.0 23 20584.0 24 20568.0 25 19885.0 26 20397.0 27 24300.0 28 30569.0 29 38216.0 30 47613.0 31 60829.0 32 77987.0 33 102301.0 34 146759.0 35 223522.0 36 410498.0 37 926370.0 38 1321369.0 39 450058.0 40 1512.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61371428457025 19.648754270635944 13.36685275778224 24.37067868701156 2 14.73305082518889 21.43201247887508 39.23145205065326 24.60348464528277 3 18.856581581581583 25.754704704704707 30.647622622622624 24.741091091091093 4 12.175812223659676 17.92198837089062 39.11931141731367 30.782887988136036 5 12.54535244146163 38.612026348836295 35.65118573266872 13.191435477033359 6 29.78117241931609 38.34037398894851 18.117442139825418 13.761011451909985 7 25.972551126611016 32.958067432747306 23.220256400695593 17.849125039946088 8 25.27904168120204 34.82013754761639 20.738223592704504 19.162597178477068 9 25.565301388134664 15.930415589550575 21.178475529340517 37.32580749297424 10-11 24.31159439330592 26.042921825251142 28.98896123607329 20.656522545369643 12-13 25.900075612895506 23.862143539877202 28.43265090703568 21.805129940191613 14-15 22.763075477019456 25.273416414519378 26.93647489959362 25.027033208867543 16-17 21.720800154854796 28.161175615174315 27.735412511732036 22.382611718238845 18-19 21.653896415570948 28.09257543657342 28.9491056820235 21.304422465832136 20-21 22.639889419543945 26.702572532138042 28.51080372907488 22.146734319243134 22-23 22.108917590838065 26.753811049616466 28.271186372813695 22.86608498673177 24-25 22.50488737271059 26.942361534449848 28.29992752774516 22.2528235650944 26-27 22.52493709654231 26.410680968952864 28.868435958968515 22.19594597553631 28-29 21.896120669322396 27.540098420637676 28.5669222923908 21.996858617649124 30-31 22.60650890239349 26.668654485831343 28.323870546676126 22.400966065099034 32-33 22.375676989150655 26.932343877705765 28.354652975649163 22.337326157494417 34-35 22.68680277256841 26.907767405754647 28.561981638666207 21.843448183010736 36-37 22.780203948489604 26.396887494497633 28.806441627133474 22.016466929879286 38-39 22.48487947814508 26.882402836953624 28.666635051532456 21.96608263336884 40-41 22.262404146153926 26.988301404469606 28.49056898880566 22.258725460570812 42-43 22.419564806111694 26.895927739956736 27.84283430580289 22.841673148128685 44-45 22.770496730347993 26.640838280166296 27.852365032854177 22.736299956631534 46-47 22.9187625062187 26.95687600537736 27.98790394022841 22.13645754817553 48-49 23.059701081679513 26.84069951811908 27.927333979311353 22.172265420890046 50-51 21.93906074356133 27.200281180336795 28.29275787960597 22.56790019649591 52-53 22.299363300402465 27.588155397995116 27.16145342933773 22.951027872264685 54-55 22.19821208596852 27.093235168440422 27.331461043206357 23.377091702384696 56-57 22.92850844600111 26.548775545366766 27.365604159255618 23.15711184937651 58-59 23.144985821352417 26.598875092640128 27.61612408090294 22.640015005104512 60-61 22.714048082787137 27.034762159899078 28.01688595772304 22.234303799590737 62-63 22.10917035212065 26.99584146906815 27.90940446108329 22.985583717727902 64-65 21.74468055289317 27.678642481803045 27.5450963689171 23.031580596386686 66-67 22.574016291198003 26.76194452824433 27.237774938522307 23.426264242035366 68-69 22.22831734718584 26.75024384323211 27.549599183664785 23.47183962591727 70-71 22.635488118098024 26.692404927391227 27.33068943485857 23.34141751965218 72-73 22.695491160404053 26.187809756239172 28.10314313428518 23.013555949071595 74-75 22.320342411571083 27.4024354505989 27.32466810297473 22.95255403485529 76-77 22.355025698617624 26.64975740379833 27.36754184823582 23.62767504934823 78-79 22.45484395006592 26.778060327854575 27.526568473042133 23.240527249037367 80-81 22.553303464032275 26.723423093473432 27.43489087553205 23.288382566962245 82-83 22.333653697085705 26.416746236247267 27.891428845275318 23.358171221391707 84-85 22.300449363604887 26.893435147208404 27.269737908142538 23.53637758104417 86-87 22.17299397499307 26.97897303611701 27.22419251994238 23.62384046894754 88-89 22.191637562649962 26.677960426545265 27.02469361953868 24.10570839126609 90-91 22.50025087518528 26.26603129971364 27.121259175712982 24.112458649388103 92-93 22.638119023726166 26.80968015595557 27.091949219346294 23.460251600971972 94-95 22.011162894755053 26.683014654755944 27.467685550561544 23.838136899927463 96-97 21.75697329102099 26.55845337221224 27.784730569117045 23.899842767649726 98-99 22.145061844251483 26.861771478821606 26.935332429430787 24.057834247496125 100 22.073683461842457 26.11130255859545 26.8852123180428 24.929801661519292 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 224.0 1 147.0 2 208.0 3 331.0 4 218.5 5 165.0 6 355.5 7 863.5 8 1468.5 9 1757.0 10 1484.5 11 1116.0 12 1099.5 13 1269.5 14 1538.0 15 1822.5 16 2120.5 17 2500.0 18 2963.5 19 3493.5 20 4184.0 21 5029.0 22 6419.5 23 8787.0 24 11382.5 25 14442.5 26 18945.0 27 24488.5 28 30860.0 29 37398.5 30 45050.0 31 52914.0 32 61513.5 33 69169.0 34 75759.0 35 83997.0 36 93004.0 37 103342.0 38 110191.0 39 113274.0 40 115547.0 41 117285.0 42 121178.0 43 126227.5 44 131713.5 45 139257.5 46 147382.0 47 169816.5 48 209120.5 49 213058.5 50 196975.0 51 180662.0 52 154560.0 53 140296.5 54 129503.0 55 116064.0 56 103884.5 57 93071.0 58 81963.0 59 72535.0 60 60873.5 61 47115.0 62 35321.0 63 26565.0 64 19328.5 65 13825.0 66 9959.5 67 7077.0 68 5091.5 69 3669.0 70 2767.5 71 2186.5 72 1720.5 73 1388.5 74 1159.5 75 941.0 76 666.0 77 407.5 78 265.0 79 158.0 80 96.5 81 54.5 82 26.0 83 19.5 84 13.5 85 10.0 86 7.5 87 5.0 88 3.5 89 2.0 90 0.5 91 1.5 92 2.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003002993985003048 2 0.0034784680326285307 3 3.002993985003048E-4 4 0.05177662129142755 5 0.0021521456892521847 6 0.004304291378504369 7 2.5024949875025403E-5 8 3.5034929825035564E-4 9 5.0049899750050806E-5 10-11 7.50748496250762E-5 12-13 1.2512474937512702E-5 14-15 2.5024949875025403E-5 16-17 2.627619736877667E-4 18-19 1.501496992501524E-4 20-21 1.876871240626905E-4 22-23 0.0 24-25 0.0 26-27 1.876871240626905E-4 28-29 1.2512474937512702E-5 30-31 2.0019959900020322E-4 32-33 2.50249498750254E-4 34-35 1.501496992501524E-4 36-37 7.50748496250762E-5 38-39 8.883857205634016E-4 40-41 4.879865225629953E-4 42-43 0.0 44-45 5.255239473755335E-4 46-47 0.0 48-49 1.0009979950010161E-4 50-51 0.0 52-53 3.628617731878683E-4 54-55 3.628617731878683E-4 56-57 2.1271207393771591E-4 58-59 9.63460570188478E-4 60-61 7.257235463757366E-4 62-63 2.377370238127413E-4 64-65 3.5034929825035564E-4 66-67 0.0012637599686887828 68-69 0.0026151072619401547 70-71 0.003628617731878683 72-73 0.0032031935840032516 74-75 0.002977969035128023 76-77 0.004366853753191932 78-79 0.0015890843170641128 80-81 0.0011886851190637065 82-83 8.383358208133508E-4 84-85 6.881861215631986E-4 86-87 0.0 88-89 0.0 90-91 2.5024949875025403E-5 92-93 5.0049899750050806E-5 94-95 1.3763722431263972E-4 96-97 3.75374248125381E-4 98-99 1.501496992501524E-4 100 0.07607584762007721 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3996012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.53255676041993 #Duplication Level Percentage of deduplicated Percentage of total 1 72.51527113602972 43.1701949490293 2 15.102082582113779 17.981311770404755 3 5.599717351117571 10.000964731431452 4 2.6688749700179084 6.355398025562206 5 1.4140482596587327 4.209095414005326 6 0.8350579380731024 2.9827880457945715 7 0.5311521422187165 2.213459153853807 8 0.3308719922064616 1.5758124525171515 9 0.22267665207122408 1.1930859385785384 >10 0.7633032893143029 6.77747210427612 >50 0.010948836413245426 0.43153883141266414 >100 0.004787634373295068 0.4992890457775051 >500 6.353770484425422E-4 0.26035374383324134 >1k 3.176885241834367E-4 0.502778143647478 >5k 1.9061311451006204E-4 0.955664294256209 >10k+ 6.353770483668735E-5 0.8907933556197792 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23550 0.5893375695568481 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9893 0.24757182911362627 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7794 0.19504445932594797 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7578 0.18963907015294248 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4320 0.10810778346010974 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4254 0.10645613676835805 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.25124749375127E-4 2 0.0 0.0 0.0 0.0 1.25124749375127E-4 3 0.0 0.0 0.0 2.50249498750254E-5 1.25124749375127E-4 4 0.0 0.0 0.0 5.00498997500508E-5 1.25124749375127E-4 5 0.0 0.0 0.0 5.00498997500508E-5 1.25124749375127E-4 6 0.0 0.0 0.0 5.00498997500508E-5 1.501496992501524E-4 7 0.0 0.0 0.0 5.00498997500508E-5 1.501496992501524E-4 8 0.0 0.0 0.0 7.50748496250762E-5 1.501496992501524E-4 9 0.0 0.0 0.0 1.751746491251778E-4 1.501496992501524E-4 10-11 0.0 0.0 0.0 2.752744486252794E-4 1.501496992501524E-4 12-13 0.0 0.0 0.0 3.378368233128429E-4 3.5034929825035564E-4 14-15 0.0 0.0 0.0 3.75374248125381E-4 4.629615726879699E-4 16-17 0.0 0.0 0.0 3.878867230628937E-4 5.755738471255842E-4 18-19 0.0 0.0 0.0 4.003991980004064E-4 6.005987970006096E-4 20-21 0.0 0.0 0.0 5.255239473755335E-4 6.881861215631984E-4 22-23 0.0 0.0 0.0 7.257235463757366E-4 7.50748496250762E-4 24-25 0.0 0.0 0.0 0.0013138098684388334 7.50748496250762E-4 26-27 0.0 0.0 0.0 0.0023273203383773625 7.757734461257875E-4 28-29 0.0 0.0 0.0 0.005668151146693253 7.882859210633002E-4 30-31 0.0 0.0 0.0 0.01542788159795316 8.007983960008128E-4 32-33 0.0 0.0 0.0 0.03633622721853688 8.007983960008128E-4 34-35 0.0 0.0 0.0 0.07115844496963472 8.007983960008128E-4 36-37 0.0 0.0 0.0 0.12112075739512293 8.258233458758382E-4 38-39 0.0 0.0 0.0 0.19600791989613645 8.508482957508637E-4 40-41 0.0 0.0 0.0 0.28626040162041555 8.75873245625889E-4 42-43 0.0 0.0 0.0 0.37175814286843983 8.883857205634017E-4 44-45 0.0 0.0 0.0 0.47946552713054913 0.001000997995001016 46-47 0.0 0.0 0.0 0.6113845503967456 0.001126122744376143 48-49 0.0 0.0 0.0 0.7475828400915713 0.00125124749375127 50-51 0.0 0.0 0.0 0.8958056182013467 0.0013263223433763462 52-53 0.0 0.0 0.0 1.049734084882628 0.0013763722431263971 54-55 0.0 0.0 0.0 1.2189402834626124 0.0014389346178139604 56-57 0.0 0.0 0.0 1.426885104449136 0.0014764720426264986 58-59 0.0 0.0 0.0 1.6550876223594924 0.001501496992501524 60-61 0.0 0.0 0.0 1.8886454795431045 0.001501496992501524 62-63 0.0 0.0 0.0 2.132513616075227 0.0015765718421266001 64-65 0.0 0.0 0.0 2.3884062410222993 0.0015765718421266001 66-67 0.0 0.0 0.0 2.679984444491158 0.001589084317064113 68-69 0.0 0.0 0.0 3.0159694215132484 0.0016015967920016256 70-71 0.0 0.0 0.0 3.386826666186187 0.001626621741876651 72-73 0.0 0.0 0.0 3.7511148615169323 0.0017392340163142654 74-75 0.0 0.0 0.0 4.117567715011868 0.0018643587656893923 76-77 0.0 0.0 0.0 4.516640590668897 0.0019269211403769558 78-79 0.0 0.0 0.0 4.974009087059798 0.001964458565189494 80-81 0.0 0.0 0.0 5.464885991333359 0.0019769710401270064 82-83 0.0 0.0 0.0 5.963182793244865 0.0021521456892521842 84-85 0.0 0.0 0.0 6.456937566754053 0.0021521456892521842 86-87 0.0 0.0 0.0 6.964656262293507 0.002202195589002235 88 0.0 0.0 0.0 7.366744644410478 0.002252245488752286 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 9855 0.0 20.840431 12-13 TCTAACG 530 0.0 19.517021 2 GTACTTT 10965 0.0 18.66644 14-15 CTAACGC 595 0.0 18.174904 3 AGTACTT 10865 0.0 18.081251 12-13 TAACGCC 640 0.0 17.631191 4 GTACTAG 1360 0.0 17.286781 1 CATGGGG 7045 0.0 17.018042 4 CTAGTAC 1925 0.0 16.853092 3 TAGGACT 2555 0.0 16.745607 4 GTATAAG 2145 0.0 16.440575 1 TATACCG 515 0.0 16.427404 5 CGCCTAC 605 0.0 16.313246 7 GGCGTCG 970 0.0 15.988903 8 ACTTTTT 12900 0.0 15.884588 16-17 GTCGACG 505 0.0 15.821399 36-37 ACTACGC 210 4.329564E-4 15.672564 3 CGATAAT 795 0.0 15.666679 66-67 CGACGAG 585 0.0 15.665895 24-25 GTACATG 21780 0.0 15.651758 1 >>END_MODULE