FastQCFastQC Report
Mon 16 Jan 2017
ERR1146618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702201
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22600.027.6746461
TATCACG1458.6305954E-725.9408662
CTGTGCG27900.021.5621519
GCTATTG27250.021.5599358
TAGTACT29050.021.5250664
GCATCGT25550.021.338858
TAGGCAT34450.021.2899365
CTAGGCA36650.021.1664964
CATGGGG87300.021.11114
CTAGTAC29950.020.8793663
GTATAAG24050.020.1400721
GGGCTAT31600.019.7827246
CAATGCG13600.019.6973418-19
CGACGAG13750.019.48390824-25
CTATTGA31350.019.1892819
GGCTATT33100.019.169977
TGATCGC17450.019.12279
TCTAACG11850.019.0451932
GTGATCG17550.019.0145118
ATGGGGG64250.018.7329355
Produced by FastQC (version 0.11.5)