##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146618_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3702201
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.391098700475744	33.0	31.0	34.0	28.0	34.0
2	31.527059173718552	33.0	31.0	34.0	28.0	34.0
3	31.554011789203233	33.0	31.0	34.0	28.0	34.0
4	35.00026686827647	37.0	35.0	37.0	32.0	37.0
5	35.00937712458076	37.0	35.0	37.0	32.0	37.0
6	35.11039703138754	37.0	35.0	37.0	32.0	37.0
7	34.98113068415248	37.0	35.0	37.0	32.0	37.0
8	35.01484603348116	37.0	35.0	37.0	32.0	37.0
9	36.589767276277	39.0	37.0	39.0	32.0	39.0
10-11	36.577699184890285	39.0	37.0	39.0	32.0	39.0
12-13	36.54653245461281	39.0	37.0	39.0	32.0	39.0
14-15	37.90130587183138	40.0	38.0	41.0	32.0	41.0
16-17	37.934683179006214	40.0	38.0	41.0	32.0	41.0
18-19	37.962110377043274	40.0	38.0	41.0	32.0	41.0
20-21	38.04336325877499	40.0	38.0	41.0	32.0	41.0
22-23	38.065045495909054	40.0	38.0	41.0	32.0	41.0
24-25	38.09705091646834	40.0	38.0	41.0	32.0	41.0
26-27	38.14806408404081	40.0	38.0	41.0	32.0	41.0
28-29	38.1241418280639	40.0	38.0	41.0	32.0	41.0
30-31	38.09642601792825	40.0	38.0	41.0	32.0	41.0
32-33	38.043976812712216	40.0	38.0	41.0	32.0	41.0
34-35	38.000857597953214	40.0	38.0	41.0	32.0	41.0
36-37	37.94011643344054	40.0	38.0	41.0	32.0	41.0
38-39	37.87728354025079	40.0	38.0	41.0	31.0	41.0
40-41	37.804763301614365	40.0	38.0	41.0	31.0	41.0
42-43	37.65208304465371	40.0	37.0	41.0	31.0	41.0
44-45	37.540556550009036	40.0	37.0	41.0	31.0	41.0
46-47	37.41576186706232	40.0	37.0	41.0	30.0	41.0
48-49	37.29168581068397	40.0	36.5	41.0	30.0	41.0
50-51	37.145038586505706	40.0	36.0	41.0	30.0	41.0
52-53	37.00910093752338	40.0	35.5	41.0	30.0	41.0
54-55	36.88566112428796	39.0	35.0	41.0	30.0	41.0
56-57	36.66486557591011	39.0	35.0	41.0	30.0	41.0
58-59	36.46175248183445	39.0	35.0	41.0	30.0	41.0
60-61	36.22881658775415	38.5	35.0	40.5	30.0	41.0
62-63	35.96393132085481	38.0	35.0	40.0	29.0	41.0
64-65	35.6848824523574	37.0	35.0	40.0	29.0	41.0
66-67	35.438694576550546	37.0	34.5	40.0	29.0	41.0
68-69	35.17663587147213	36.0	34.0	39.5	29.0	41.0
70-71	34.89233445185715	36.0	34.0	39.0	28.5	41.0
72-73	34.558315985544816	35.0	34.0	39.0	28.0	41.0
74-75	34.23420932574974	35.0	34.0	37.5	28.0	40.0
76-77	33.95295960430025	35.0	34.0	37.0	28.0	39.0
78-79	33.64107324264674	35.0	34.0	37.0	27.0	39.0
80-81	33.406074926780036	35.0	33.0	36.0	27.0	39.0
82-83	33.1335752704945	35.0	33.0	36.0	26.5	37.0
84-85	32.92802092593028	35.0	33.0	35.5	26.0	37.0
86-87	32.71963569779167	35.0	33.0	35.0	26.0	37.0
88-89	32.537464335404806	35.0	33.0	35.0	26.0	36.0
90-91	32.37241684068477	35.0	33.0	35.0	25.0	36.0
92-93	32.185179032688936	35.0	33.0	35.0	25.0	36.0
94-95	32.059232467388995	35.0	33.0	35.0	25.0	36.0
96-97	31.95686430855591	35.0	33.0	35.0	25.0	35.0
98-99	31.83575999790395	35.0	33.0	35.0	24.0	35.0
100	30.64290512589673	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30989506641709497
1101	2	0.35545221185104126
1101	3	0.3779517354864943
1101	4	0.33314580652697856
1101	5	0.3077066734921061
1101	6	0.38573364498743956
1101	7	0.40628433629526484
1101	8	0.4372670665187357
1101	9	0.5670107148822225
1101	10-11	0.5443292479505644
1101	12-13	0.5427946233812051
1101	14-15	0.6604459067749389
1101	16-17	0.5976028500193635
1101	18-19	0.6262598077582666
1101	20-21	0.6733378803071801
1101	22-23	0.6247067889768232
1101	24-25	0.6178459980947295
1101	26-27	0.5729733720834815
1101	28-29	0.542896315694513
1101	30-31	0.5944864137598742
1101	32-33	0.6298707807537554
1101	34-35	0.5091164866283648
1101	36-37	0.5207227381565218
1101	38-39	0.5564787614084636
1101	40-41	0.4938132223385523
1101	42-43	0.5266195619590803
1101	44-45	0.5285846210297365
1101	46-47	0.4738046530272797
1101	48-49	0.44746088870655853
1101	50-51	0.4450500719449195
1101	52-53	0.4429686906141228
1101	54-55	0.36438285863969355
1101	56-57	0.40357258902366056
1101	58-59	0.41464318446715964
1101	60-61	0.37775015594402106
1101	62-63	0.33516477281864354
1101	64-65	0.3875658921177987
1101	66-67	0.23506941634013145
1101	68-69	0.26660603546819317
1101	70-71	0.2650556548702596
1101	72-73	0.27287984283978517
1101	74-75	0.3151713788134103
1101	76-77	0.28812759997308035
1101	78-79	0.37251257415253747
1101	80-81	0.2767445233450232
1101	82-83	0.33166452053004747
1101	84-85	0.2659187745775995
1101	86-87	0.2916182943297372
1101	88-89	0.31320807641725423
1101	90-91	0.3397376061035402
1101	92-93	0.3984882574756128
1101	94-95	0.34096048821447766
1101	96-97	0.40947438631582855
1101	98-99	0.41319105386205024
1101	100	0.4806861446350936
1102	1	0.3584506041891835
1102	2	0.4071692946508705
1102	3	0.35832518832473426
1102	4	0.37647060379543973
1102	5	0.3431284084472921
1102	6	0.45594304532887975
1102	7	0.37392091333325084
1102	8	0.4432260400569348
1102	9	0.5221136811647611
1102	10-11	0.5421589534563296
1102	12-13	0.46680604036797746
1102	14-15	0.5511353273895381
1102	16-17	0.46467778066383403
1102	18-19	0.5572514584198132
1102	20-21	0.5917959202132863
1102	22-23	0.5569593764714824
1102	24-25	0.5945005275441559
1102	26-27	0.5256292886434508
1102	28-29	0.5943499808673707
1102	30-31	0.6105630249421168
1102	32-33	0.6157633330371439
1102	34-35	0.5794882055784285
1102	36-37	0.5444121854463191
1102	38-39	0.5707941423303566
1102	40-41	0.5738928478187049
1102	42-43	0.6526092119804261
1102	44-45	0.5860535444185544
1102	46-47	0.5449794289555783
1102	48-49	0.5361519365085243
1102	50-51	0.5606653354959192
1102	52-53	0.5811167646644861
1102	54-55	0.5469342084049487
1102	56-57	0.6207928995230176
1102	58-59	0.5989122839123198
1102	60-61	0.5864458719064629
1102	62-63	0.5971422055971374
1102	64-65	0.642804768558257
1102	66-67	0.60739829491461
1102	68-69	0.5889129070004273
1102	70-71	0.51595247518609
1102	72-73	0.6229616023361544
1102	74-75	0.6114477872641189
1102	76-77	0.6019233479457569
1102	78-79	0.5187967184120339
1102	80-81	0.4215452595806184
1102	82-83	0.4830270898855744
1102	84-85	0.4844723117094816
1102	86-87	0.4576656961649874
1102	88-89	0.42821434508989853
1102	90-91	0.4113819989759264
1102	92-93	0.3600863688712579
1102	94-95	0.3831062415222064
1102	96-97	0.36035840253434515
1102	98-99	0.39860812065675333
1102	100	0.21670193635511126
1103	1	0.16027941825354475
1103	2	0.23691129902115193
1103	3	0.21280075447491242
1103	4	0.11298629708109331
1103	5	0.19528598691372423
1103	6	0.1646192968187492
1103	7	0.19065611396892734
1103	8	0.14683127446297561
1103	9	0.2969175150940444
1103	10-11	0.2838613769763896
1103	12-13	0.33590963591823453
1103	14-15	0.4145854912751119
1103	16-17	0.322365628299913
1103	18-19	0.3548953549235847
1103	20-21	0.31572458032453454
1103	22-23	0.3534578121091485
1103	24-25	0.3518089552358461
1103	26-27	0.2627137393784551
1103	28-29	0.33086654324321785
1103	30-31	0.33357355334895544
1103	32-33	0.3566339130927716
1103	34-35	0.31633253911041237
1103	36-37	0.3573509518348743
1103	38-39	0.34159159465455957
1103	40-41	0.31166412811270305
1103	42-43	0.2903997364142725
1103	44-45	0.28809205752939704
1103	46-47	0.2573403745257039
1103	48-49	0.2633035554993839
1103	50-51	0.321305110029094
1103	52-53	0.32632422890763024
1103	54-55	0.26037741956903204
1103	56-57	0.26605648216108335
1103	58-59	0.338241842911124
1103	60-61	0.353079885613262
1103	62-63	0.302565576673814
1103	64-65	0.3001968772193564
1103	66-67	0.2816773225268463
1103	68-69	0.23556169524038495
1103	70-71	0.3126653277884017
1103	72-73	0.27106859975371833
1103	74-75	0.3298280514675298
1103	76-77	0.2878111856493746
1103	78-79	0.2986412754243588
1103	80-81	0.20041122104267828
1103	82-83	0.24893590990941306
1103	84-85	0.23607230674324597
1103	86-87	0.23664575675299204
1103	88-89	0.2083656909381304
1103	90-91	0.2651688732309907
1103	92-93	0.2914677256451981
1103	94-95	0.2808207826425857
1103	96-97	0.3681042309014515
1103	98-99	0.28341679665993524
1103	100	0.344943891063096
1104	1	0.26159855199930604
1104	2	0.218307669945915
1104	3	0.23726172123845046
1104	4	0.23021830901442542
1104	5	0.19547580550997168
1104	6	0.240382627023088
1104	7	0.2676535385627261
1104	8	0.25199718482483036
1104	9	0.36164456236067366
1104	10-11	0.3653257512533372
1104	12-13	0.387407600819067
1104	14-15	0.5103535446725189
1104	16-17	0.5036668476714397
1104	18-19	0.49399147612240313
1104	20-21	0.45561533733891224
1104	22-23	0.4249285772320377
1104	24-25	0.4654670401326655
1104	26-27	0.43428036719052443
1104	28-29	0.4271187161152099
1104	30-31	0.45799483716347567
1104	32-33	0.4356272853056993
1104	34-35	0.3883059835747602
1104	36-37	0.3920326614494627
1104	38-39	0.34350255762015536
1104	40-41	0.3696364388231572
1104	42-43	0.4401436336956053
1104	44-45	0.3669192505554548
1104	46-47	0.33782299045571307
1104	48-49	0.4197844109381066
1104	50-51	0.35977635372304206
1104	52-53	0.3269460821310517
1104	54-55	0.33692675036665776
1104	56-57	0.2852941944351599
1104	58-59	0.2796905425013563
1104	60-61	0.31810016314695844
1104	62-63	0.3510120259485632
1104	64-65	0.33778009966237477
1104	66-67	0.3030002651480217
1104	68-69	0.21728557943682603
1104	70-71	0.24337478045753613
1104	72-73	0.28669889908412216
1104	74-75	0.26998912610492454
1104	76-77	0.23380236381252928
1104	78-79	0.246653720772386
1104	80-81	0.20369858373482685
1104	82-83	0.18854964430467192
1104	84-85	0.17188568372625923
1104	86-87	0.17077815682588238
1104	88-89	0.19342046559868464
1104	90-91	0.1574275175964317
1104	92-93	0.1650055597970379
1104	94-95	0.17607831075903135
1104	96-97	0.15810066584408133
1104	98-99	0.17731476568691562
1104	100	0.2809859646792674
1105	1	0.22740417107828392
1105	2	0.2620721783496336
1105	3	0.2768078702440242
1105	4	0.32318493490443245
1105	5	0.2914599543640364
1105	6	0.32333480732443576
1105	7	0.2649592037685835
1105	8	0.21672639754024914
1105	9	0.2942836049016577
1105	10-11	0.35598640817510585
1105	12-13	0.317170276148083
1105	14-15	0.42891605109465303
1105	16-17	0.3762633184776192
1105	18-19	0.4360440585151366
1105	20-21	0.3951265724217379
1105	22-23	0.32892742143494047
1105	24-25	0.3742922935117363
1105	26-27	0.37090683471907937
1105	28-29	0.3135834770542161
1105	30-31	0.34386219875845114
1105	32-33	0.4354674162910399
1105	34-35	0.3442015238180218
1105	36-37	0.37003122739102423
1105	38-39	0.3573091764898777
1105	40-41	0.40119811871161914
1105	42-43	0.3776747505886249
1105	44-45	0.3855003389848122
1105	46-47	0.33823888240331
1105	48-49	0.36503956758573963
1105	50-51	0.3831278990485103
1105	52-53	0.35604616170929404
1105	54-55	0.2850763874643647
1105	56-57	0.30092685646614115
1105	58-59	0.26739576023897627
1105	60-61	0.30527402173169804
1105	62-63	0.24131741214305436
1105	64-65	0.3193329104394067
1105	66-67	0.2613765877331673
1105	68-69	0.255806774175511
1105	70-71	0.22517196994301258
1105	72-73	0.2042754528286821
1105	74-75	0.2002488279377701
1105	76-77	0.23165652636966172
1105	78-79	0.2336144019201356
1105	80-81	0.20577607464258563
1105	82-83	0.1776783450492232
1105	84-85	0.16198829915784074
1105	86-87	0.20624440207538441
1105	88-89	0.17002211836603465
1105	90-91	0.18460361877255593
1105	92-93	0.21168888714262835
1105	94-95	0.16705047412468588
1105	96-97	0.17454135455214725
1105	98-99	0.16415199180387674
1105	100	0.24715474855720743
1106	1	0.07404650414629899
1106	2	0.15656060599069832
1106	3	0.16360516136890624
1106	4	0.09425471210885661
1106	5	-0.004645017087227643
1106	6	0.15880629810104097
1106	7	0.10727124721795178
1106	8	0.17237819499168694
1106	9	0.15836735695965132
1106	10-11	0.15870830100273992
1106	12-13	0.213437557246003
1106	14-15	0.20168568195058612
1106	16-17	0.23448034070181478
1106	18-19	0.22932110324613575
1106	20-21	0.16021373939481265
1106	22-23	0.244584257773937
1106	24-25	0.33623257358348013
1106	26-27	0.31551141432500174
1106	28-29	0.2641962292592126
1106	30-31	0.2340717243233641
1106	32-33	0.263577996395604
1106	34-35	0.258488714829177
1106	36-37	0.2581356508019468
1106	38-39	0.2510499355362654
1106	40-41	0.21006372250473504
1106	42-43	0.20474002433109462
1106	44-45	0.1771631175668773
1106	46-47	0.21633196831722046
1106	48-49	0.21445153676582862
1106	50-51	0.24744823003124594
1106	52-53	0.20393581217413725
1106	54-55	0.1660085781592855
1106	56-57	0.2304102531038552
1106	58-59	0.2008823568155833
1106	60-61	0.2606184925950643
1106	62-63	0.19986685027029694
1106	64-65	0.2616589352249221
1106	66-67	0.23679100382229024
1106	68-69	0.17158367830471377
1106	70-71	0.1652708449710616
1106	72-73	0.1777127012456674
1106	74-75	0.22404423859046574
1106	76-77	0.19091941561102033
1106	78-79	0.18904001008635873
1106	80-81	0.22717347590114656
1106	82-83	0.2561881203334053
1106	84-85	0.2610596616145173
1106	86-87	0.1752693650677628
1106	88-89	0.20314980808689143
1106	90-91	0.23266521514329952
1106	92-93	0.22592831734886687
1106	94-95	0.24364979969166
1106	96-97	0.25886380901600603
1106	98-99	0.2331390930576589
1106	100	0.2956037926315318
1107	1	0.0521142276561406
1107	2	0.08505266917036636
1107	3	0.05224519564671937
1107	4	0.08333313354099658
1107	5	0.00442382763089455
1107	6	0.10107381139148686
1107	7	0.03837771362613296
1107	8	0.04082591649844858
1107	9	0.16090074871006976
1107	10-11	0.1351911701870634
1107	12-13	0.14426187965042203
1107	14-15	0.0936741682278992
1107	16-17	0.10505274634203232
1107	18-19	0.11009999631107803
1107	20-21	0.2742007916209346
1107	22-23	0.25972474119782873
1107	24-25	0.2610891121130692
1107	26-27	0.1827686237157593
1107	28-29	0.1668868768036731
1107	30-31	0.12939562607702726
1107	32-33	0.17895274361741542
1107	34-35	0.15677066256176886
1107	36-37	0.12508134616387423
1107	38-39	0.11205060626959096
1107	40-41	0.10263135001529378
1107	42-43	0.19138026268516484
1107	44-45	0.1529445040561157
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2216	52-53	-0.7252264003257309
2216	54-55	-0.768930994864121
2216	56-57	-0.6962686924021
2216	58-59	-0.7821585459284179
2216	60-61	-0.8289810106026181
2216	62-63	-0.8066635013042287
2216	64-65	-0.755454783690972
2216	66-67	-0.7126953939649638
2216	68-69	-0.8089621977774542
2216	70-71	-0.7841638282506196
2216	72-73	-0.7130233969653261
2216	74-75	-0.7753734267621439
2216	76-77	-0.7927831867007882
2216	78-79	-0.7055449272990586
2216	80-81	-0.7162266389944065
2216	82-83	-0.6768786100305988
2216	84-85	-0.666497844060288
2216	86-87	-0.7107578334522202
2216	88-89	-0.7320052518645568
2216	90-91	-0.7313720000461501
2216	92-93	-0.6435227048923622
2216	94-95	-0.621676519025435
2216	96-97	-0.630652005400826
2216	98-99	-0.641813348328995
2216	100	-0.7095917210911011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1126.0
1	256.0
2	189.0
3	178.0
4	142.0
5	224.0
6	373.0
7	1157.0
8	3072.0
9	3795.0
10	3579.0
11	4061.0
12	4723.0
13	6301.0
14	9020.0
15	10183.0
16	10689.0
17	11350.0
18	12489.0
19	13838.0
20	15249.0
21	16763.0
22	19112.0
23	22260.0
24	27820.0
25	33837.0
26	36404.0
27	34232.0
28	36591.0
29	44752.0
30	52006.0
31	61948.0
32	79265.0
33	105552.0
34	157745.0
35	246465.0
36	446336.0
37	875880.0
38	961398.0
39	331205.0
40	636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54597995764847	20.464785289874353	13.052186684143702	23.937048068333475
2	14.666226285715522	21.259453482554726	39.96824716398902	24.106073067740734
3	18.147543013284473	27.404880939315085	30.24943731168256	24.19813873571788
4	12.232474185139258	18.238293392850046	39.439446421035875	30.089786000974822
5	12.738932020803883	39.3542567050461	35.07977714681639	12.827034127333631
6	29.219878787616622	38.20693593079047	19.05922385034918	13.513961431243725
7	25.503663065555614	33.59706576269867	23.448512835125417	17.4507583366203
8	25.35567369290729	35.07860183146539	21.239094043195593	18.32663043243173
9	26.385617757878126	16.044650949559813	21.11337601173796	36.4563552808241
10-11	24.221255730836308	26.962473945214565	28.243718568173215	20.572551755775915
12-13	25.21005064353181	24.945629022073835	27.731198967235237	22.11312136715912
14-15	23.204119320927308	25.934510247413634	25.516979723325033	25.344390708334025
16-17	21.886448928780695	28.54541116966024	27.06909450694716	22.4990453946119
18-19	21.8033301619179	28.871743391356404	27.957043022532368	21.367883424193327
20-21	23.1514425955394	28.02786395199196	26.77254809845638	22.04814535401226
22-23	22.999583170467062	27.78745964836023	26.095583174792143	23.117374006380572
24-25	22.78912534656899	28.178375392422268	26.467281170394557	22.565218090614184
26-27	22.95447766307651	27.61386057923562	26.56581737031099	22.865844387376878
28-29	22.35210188382954	28.11015428092637	26.873263050445996	22.66448078479809
30-31	22.958205007072728	27.910547725879564	26.599082065953105	22.5321652010946
32-33	22.609846959051204	28.440569233389475	26.285154676521554	22.664429131037764
34-35	22.684420382077786	28.363504186980666	26.905181301554933	22.04689412938662
36-37	23.00237339098609	27.69170627049412	26.686333631347686	22.619586707172108
38-39	23.233563304282143	27.613302754649858	26.796824330005382	22.356309611062613
40-41	22.837045971360713	27.440075512106688	27.205688441730146	22.517190074802457
42-43	22.94878690677686	27.29659477410354	26.830447307722622	22.924171011396986
44-45	22.82042145763742	27.84716942977671	26.912125855507963	22.420283257077912
46-47	22.60171494201727	27.852189013173696	26.878310540025474	22.66778550478356
48-49	23.005950815907	27.452955894533176	27.021389601557182	22.519703688002647
50-51	22.397422014073808	27.449591515648148	26.947463357932378	23.205523112345666
52-53	22.12940598675759	27.954280031898154	26.388626217725864	23.527687763618392
54-55	22.37569800119223	27.845053535390402	26.37876102845263	23.400487434964738
56-57	23.04748654290879	27.186953904486185	26.536847198090246	23.228712354514784
58-59	22.560850148693287	27.21400774843567	27.187957019948072	23.03718508292297
60-61	22.675721818112258	27.664779147862788	26.91053317685948	22.74896585716547
62-63	22.399637805620095	27.38029720433579	26.717432280858	23.502632709186113
64-65	21.688576627632507	27.664777597222635	26.939058583684606	23.707587191460252
66-67	22.987207420301896	27.008223028698403	26.54924002147959	23.45532952952011
68-69	22.65370094509387	26.91071283484495	26.81590467974838	23.619681540312797
70-71	22.35059742643331	27.032805931787045	26.9119308726068	23.704665769172845
72-73	22.891810092165798	26.761270356615235	27.250096794637468	23.096822756581496
74-75	22.688044075019572	27.500556656400576	26.849427519280862	22.961971749298993
76-77	22.455606898922664	27.106440833960725	26.964749002945638	23.473203264170976
78-79	22.494109764224277	27.134373086526807	26.99588984775767	23.375627301491246
80-81	22.377949958387884	27.038588140627862	27.454817460974105	23.128644440010145
82-83	22.56264521000485	26.61587766836587	27.73027932371767	23.09119779791161
84-85	22.679149499954374	27.117526776691353	26.76217160741264	23.441152115941637
86-87	22.200216678747935	27.18572050820819	27.266427528745623	23.347635284298253
88-89	22.771906468089984	26.415702424817255	27.149482451140816	23.66290865595195
90-91	22.833763972102233	26.34595400310201	27.189386832849564	23.6308951919462
92-93	22.85789948525728	26.57658376188006	27.21900506145199	23.346511691410672
94-95	22.103738181692343	26.647364720669486	27.646306664375814	23.60259043326236
96-97	22.52685903101208	26.43625084451947	27.44911998300012	23.58777014146833
98-99	22.413708256779028	26.787098588426478	27.145447097425745	23.65374605736875
100	22.715206397015937	26.443441526125426	26.70218578791094	24.1391662889477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3348.0
1	2247.5
2	1199.5
3	1211.5
4	1085.5
5	863.0
6	689.0
7	673.0
8	778.0
9	834.0
10	711.0
11	589.5
12	567.5
13	594.5
14	642.0
15	732.5
16	863.0
17	1031.0
18	1261.0
19	1586.0
20	2140.5
21	2915.0
22	3925.0
23	5388.0
24	7445.5
25	10290.5
26	13601.0
27	17312.0
28	22683.5
29	30569.5
30	38815.5
31	46922.0
32	58168.5
33	68279.0
34	76786.5
35	85517.5
36	94195.5
37	105597.5
38	109757.5
39	106996.5
40	104382.5
41	100310.0
42	100029.0
43	107698.5
44	120184.5
45	135736.0
46	144719.5
47	153518.5
48	170310.5
49	174849.0
50	182651.5
51	179518.0
52	155457.5
53	143117.5
54	133630.5
55	117100.5
56	103733.5
57	89208.5
58	74308.0
59	66525.5
60	54687.5
61	40710.0
62	30970.0
63	24782.0
64	18369.0
65	12050.0
66	8366.5
67	6096.5
68	4566.5
69	3446.5
70	2855.5
71	2522.5
72	2127.5
73	1774.5
74	1320.5
75	977.0
76	711.0
77	479.0
78	334.0
79	219.5
80	132.5
81	86.5
82	79.0
83	75.5
84	55.5
85	38.5
86	39.0
87	35.0
88	19.5
89	12.0
90	14.0
91	13.0
92	10.5
93	12.0
94	12.5
95	11.5
96	11.0
97	9.5
98	8.5
99	11.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09813081461541391
2	0.06412401703743259
3	0.08089782267359336
4	0.08297766652864066
5	0.08330179803851817
6	0.09194530496858491
7	0.08778561725849028
8	0.08927122001209552
9	0.07428013768026101
10-11	0.08169464596870889
12-13	0.07068768011245202
14-15	0.07969583499113095
16-17	0.08180268980533471
18-19	0.08134350349967492
20-21	0.07168708560124099
22-23	0.07690020071843749
24-25	0.07837229799246448
26-27	0.07259195273298236
28-29	0.0812354596630491
30-31	0.08463884051676286
32-33	0.08619197066825923
34-35	0.08898760494095269
36-37	0.0884338802782453
38-39	0.08989247207269405
40-41	0.08620547614783747
42-43	0.091067448796
44-45	0.08721838711620467
46-47	0.0844227528435112
48-49	0.09357946799755064
50-51	0.09033815289877563
52-53	0.09601045432163192
54-55	0.08767757342186445
56-57	0.09455186252718314
58-59	0.09853597900276079
60-61	0.09741502419776776
62-63	0.09817133105414859
64-65	0.10077788861274685
66-67	0.10283072150863772
68-69	0.09938682421618923
70-71	0.10139914067334539
72-73	0.09856298996191726
74-75	0.10208792013183508
76-77	0.09944084613450214
78-79	0.1058694544137393
80-81	0.10153419546912769
82-83	0.10516716947567137
84-85	0.1023310187642432
86-87	0.09826586941119621
88-89	0.09759059543228474
90-91	0.09792823242174048
92-93	0.09345791868134658
94-95	0.09285017210032626
96-97	0.09054073509244906
98-99	0.10388414891573958
100	0.24253140226584133
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3702201.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.24756206381879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.38987805930086	38.1847927868656
2	14.581316351075872	15.820017274543282
3	5.934755996132151	9.658381327013988
4	2.9779280648664255	6.4618135008171675
5	1.8025428263593126	4.889177692280907
6	1.109847331217975	3.612390718896645
7	0.7517309811688347	2.8545701139376227
8	0.5192244743062171	2.253332951598416
9	0.36806157753318275	1.796979894348455
>10	1.537557971715139	12.978600801802202
>50	0.02274306443900808	0.8094467759072027
>100	0.004148815244268869	0.3537977700863275
>500	0.0	0.0
>1k	2.6448664082912E-4	0.3266983919022701
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.701095915645855E-5	0.0
3	0.0	0.0	0.0	2.701095915645855E-5	0.0
4	0.0	0.0	0.0	2.701095915645855E-5	0.0
5	0.0	0.0	0.0	2.701095915645855E-5	0.0
6	0.0	0.0	0.0	2.701095915645855E-5	0.0
7	0.0	0.0	0.0	2.701095915645855E-5	0.0
8	0.0	0.0	0.0	2.701095915645855E-5	0.0
9	0.0	0.0	0.0	2.701095915645855E-5	0.0
10-11	0.0	0.0	0.0	2.701095915645855E-5	0.0
12-13	0.0	0.0	0.0	2.701095915645855E-5	0.0
14-15	0.0	0.0	0.0	5.40219183129171E-5	0.0
16-17	0.0	0.0	0.0	9.453835704760492E-5	0.0
18-19	0.0	0.0	0.0	1.3505479578229275E-4	0.0
20-21	0.0	0.0	0.0	2.971205507210441E-4	0.0
22-23	0.0	0.0	0.0	9.048671317413614E-4	0.0
24-25	0.0	0.0	0.0	0.002066338375469079	0.0
26-27	0.0	0.0	0.0	0.0036734904452783625	0.0
28-29	0.0	0.0	0.0	0.009764461735059765	0.0
30-31	0.0	0.0	0.0	0.02555236736200979	0.0
32-33	0.0	0.0	0.0	0.06039650467384132	0.0
34-35	0.0	0.0	0.0	0.11220352433592881	0.0
36-37	0.0	0.0	0.0	0.19000859218610766	0.0
38-39	0.0	0.0	0.0	0.3114228535943888	0.0
40-41	0.0	0.0	0.0	0.45209592888122496	0.0
42-43	0.0	0.0	0.0	0.5826533999639674	0.0
44-45	0.0	0.0	0.0	0.746447856288732	0.0
46-47	0.0	0.0	0.0	0.943128155386485	0.0
48-49	0.0	0.0	0.0	1.1544348888674603	0.0
50-51	0.0	0.0	0.0	1.3784232676723929	0.0
52-53	0.0	0.0	0.0	1.6066118506261544	0.0
54-55	0.0	0.0	0.0	1.85704395844526	0.0
56-57	0.0	0.0	0.0	2.150477513241447	0.0
58-59	0.0	0.0	0.0	2.483198508130704	0.0
60-61	0.0	0.0	0.0	2.823955263369007	0.0
62-63	0.0	0.0	0.0	3.1798516612145047	0.0
64-65	0.0	0.0	0.0	3.5631911935629645	0.0
66-67	0.0	0.0	0.0	3.9824823125486706	0.0
68-69	0.0	0.0	0.0	4.451743705973825	0.0
70-71	0.0	0.0	0.0	4.975405171140087	0.0
72-73	0.0	0.0	0.0	5.488059670450092	0.0
74-75	0.0	0.0	0.0	5.989166984720711	0.0
76-77	0.0	0.0	0.0	6.548523432412233	0.0
78-79	0.0	0.0	0.0	7.16580488201478	0.0
80-81	0.0	0.0	0.0	7.831611519741905	0.0
82-83	0.0	0.0	0.0	8.521093263169666	0.0
84-85	0.0	0.0	0.0	9.192639729717538	0.0
86-87	0.0	0.0	0.0	9.89408732805161	0.0
88	0.0	0.0	0.0	10.455888267546792	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2260	0.0	27.674646	1
TATCACG	145	8.6305954E-7	25.940866	2
CTGTGCG	2790	0.0	21.562151	9
GCTATTG	2725	0.0	21.559935	8
TAGTACT	2905	0.0	21.525066	4
GCATCGT	2555	0.0	21.33885	8
TAGGCAT	3445	0.0	21.289936	5
CTAGGCA	3665	0.0	21.166496	4
CATGGGG	8730	0.0	21.1111	4
CTAGTAC	2995	0.0	20.879366	3
GTATAAG	2405	0.0	20.140072	1
GGGCTAT	3160	0.0	19.782724	6
CAATGCG	1360	0.0	19.69734	18-19
CGACGAG	1375	0.0	19.483908	24-25
CTATTGA	3135	0.0	19.189281	9
GGCTATT	3310	0.0	19.16997	7
TGATCGC	1745	0.0	19.1227	9
TCTAACG	1185	0.0	19.045193	2
GTGATCG	1755	0.0	19.014511	8
ATGGGGG	6425	0.0	18.732935	5
>>END_MODULE