FastQCFastQC Report
Mon 16 Jan 2017
ERR1146618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702201
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142360.38452801455134394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130840.3534113896031037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73620.19885468130984787No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG67010.18100043730742874No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62440.16865642897292718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT149300.023.59338812-13
GTGATCG17000.022.3934218
GGGCTAT30300.021.8714926
GCTATTG28100.021.575848
AGTACTT156450.021.55384812-13
GTACTAG20400.021.4365161
TAGTACT28850.021.1878644
GTACTTT167650.021.08107414-15
TGATCGC18050.020.8300939
CTGTGCG26400.020.8286159
TCTAACG11100.020.7574732
CTAACGC11100.020.7569123
GTATAAG24850.020.4361341
AGGGCTA31550.020.4093425
GTACCCG3252.5465852E-1120.2556441
CATGGGG83850.020.1317274
TATTCTC18350.019.9786575
GGCTATT31600.019.7810297
ACTTTTT183750.019.31070116-17
ATAAGGT22950.019.0541653
Produced by FastQC (version 0.11.5)