FastQCFastQC Report
Mon 16 Jan 2017
ERR1146617_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146617_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283526
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.039.7868421
GTATCAA52800.034.2063521
CAACGCA54250.031.107545
ATCAACG55250.030.6305243
TATCAAC64100.027.7211592
AACGCAG65650.026.911556
TCAACGC67500.025.210174
GTACATG113900.025.0653881
ACGCAGA68200.024.9407657
GTACTAG18150.024.8773461
TACATGG113150.024.6779752
CGCAGAG69550.024.3890938
ACATGGG111050.024.1713963
CGTTAAC5050.023.2840271
CATGGGG93300.021.8161054
TGATCGC9650.020.450369
GCAGAGT81100.020.2201129
GTGATCG9950.019.8340688
ATGGGGG67800.019.4132985
AGAGTAC68200.019.36127910-11
Produced by FastQC (version 0.11.5)