##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146617_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3283526
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38348775066803	31.0	31.0	34.0	28.0	34.0
2	31.47710114066403	33.0	31.0	34.0	28.0	34.0
3	31.636222767841645	33.0	31.0	34.0	28.0	34.0
4	35.11778466197618	37.0	35.0	37.0	32.0	37.0
5	35.08676313207204	37.0	35.0	37.0	32.0	37.0
6	35.173097456819285	37.0	35.0	37.0	32.0	37.0
7	35.03250895531206	37.0	35.0	37.0	32.0	37.0
8	35.026428601448565	37.0	35.0	37.0	32.0	37.0
9	36.641204912036635	39.0	37.0	39.0	32.0	39.0
10-11	36.6455019695291	39.0	37.0	39.0	32.0	39.0
12-13	36.60355300978277	39.0	37.0	39.0	32.0	39.0
14-15	37.955484744143945	40.0	38.0	41.0	32.0	41.0
16-17	37.98048439391069	40.0	38.0	41.0	32.0	41.0
18-19	37.99673750108877	40.0	38.0	41.0	32.0	41.0
20-21	38.09776761323041	40.0	38.0	41.0	32.0	41.0
22-23	38.108676313207205	40.0	38.0	41.0	32.0	41.0
24-25	38.12981456519607	40.0	38.0	41.0	32.0	41.0
26-27	38.185776814314856	40.0	38.0	41.0	32.5	41.0
28-29	38.1667711783004	40.0	38.0	41.0	32.5	41.0
30-31	38.13676441118481	40.0	38.0	41.0	32.0	41.0
32-33	38.10803736592919	40.0	38.0	41.0	32.0	41.0
34-35	38.060453000828986	40.0	38.0	41.0	32.0	41.0
36-37	37.98840606104535	40.0	38.0	41.0	32.0	41.0
38-39	37.931628529818255	40.0	38.0	41.0	32.0	41.0
40-41	37.87292943622191	40.0	38.0	41.0	32.0	41.0
42-43	37.71968335259109	40.0	37.5	41.0	31.0	41.0
44-45	37.59208667755334	40.0	37.0	41.0	31.0	41.0
46-47	37.4783695941497	40.0	37.0	41.0	31.0	41.0
48-49	37.36486523937987	40.0	36.5	41.0	31.0	41.0
50-51	37.228555065499705	40.0	36.0	41.0	30.0	41.0
52-53	37.08976645837432	40.0	36.0	41.0	30.0	41.0
54-55	36.97078932830135	39.0	35.0	41.0	30.0	41.0
56-57	36.775760112756835	39.0	35.0	41.0	30.0	41.0
58-59	36.57053119116462	39.0	35.0	41.0	30.0	41.0
60-61	36.3549508972976	38.5	35.0	40.5	30.0	41.0
62-63	36.123387328134456	38.0	35.0	40.0	30.0	41.0
64-65	35.835278143069374	37.0	35.0	40.0	29.0	41.0
66-67	35.59944629645082	37.0	35.0	40.0	29.0	41.0
68-69	35.34960283548844	36.0	34.0	39.5	29.0	41.0
70-71	35.08772216209039	36.0	34.0	39.0	29.0	41.0
72-73	34.754913772572536	35.5	34.0	39.0	29.0	41.0
74-75	34.42829461377799	35.0	34.0	38.5	28.0	40.0
76-77	34.15866190796113	35.0	34.0	37.0	28.0	39.5
78-79	33.85104153279127	35.0	34.0	37.0	28.0	39.0
80-81	33.61305894943423	35.0	34.0	36.0	28.0	39.0
82-83	33.33855267173155	35.0	33.5	36.0	27.0	37.0
84-85	33.13652137976066	35.0	33.0	36.0	27.0	37.0
86-87	32.926779778810946	35.0	33.0	35.0	27.0	37.0
88-89	32.75266238184196	35.0	33.0	35.0	27.0	36.0
90-91	32.60203238835325	35.0	33.0	35.0	26.0	36.0
92-93	32.45145797535942	35.0	33.0	35.0	26.0	36.0
94-95	32.33297817650903	35.0	33.0	35.0	26.0	36.0
96-97	32.236705754728305	35.0	33.0	35.0	25.0	35.0
98-99	32.123884659357046	35.0	33.0	35.0	25.0	35.0
100	30.979062751444637	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37810235619194543
1101	2	0.36180092315480294
1101	3	0.37264309484753255
1101	4	0.3820698395445916
1101	5	0.2749458077061817
1101	6	0.3640283750086297
1101	7	0.3679203974319236
1101	8	0.36792480314723974
1101	9	0.4959902457005114
1101	10-11	0.4947396274175091
1101	12-13	0.5110769283270287
1101	14-15	0.555389221395572
1101	16-17	0.6127701767312246
1101	18-19	0.538385884990106
1101	20-21	0.571737083877693
1101	22-23	0.6000782408495127
1101	24-25	0.6691920320895761
1101	26-27	0.6114091219299169
1101	28-29	0.6490962633173183
1101	30-31	0.6644635359406763
1101	32-33	0.6319323813852478
1101	34-35	0.6042871159526797
1101	36-37	0.6512996776502717
1101	38-39	0.6514223696170376
1101	40-41	0.5854603609352651
1101	42-43	0.5968976980271279
1101	44-45	0.608520176082628
1101	46-47	0.5941618869872585
1101	48-49	0.6219976951783082
1101	50-51	0.6307033228268111
1101	52-53	0.5652232490527709
1101	54-55	0.559408915619592
1101	56-57	0.6461348033881791
1101	58-59	0.6552519304508806
1101	60-61	0.6166262536515674
1101	62-63	0.6160582022049539
1101	64-65	0.5958438989281163
1101	66-67	0.48375705287956805
1101	68-69	0.5187512872144566
1101	70-71	0.47078788885929157
1101	72-73	0.5257198243490251
1101	74-75	0.49414686252135454
1101	76-77	0.4863634805975252
1101	78-79	0.46317696803569675
1101	80-81	0.47815832752659304
1101	82-83	0.4371877183004642
1101	84-85	0.4100954764973892
1101	86-87	0.4870663173415224
1101	88-89	0.4800842424392613
1101	90-91	0.4342668534036349
1101	92-93	0.43208586327158116
1101	94-95	0.44151643273393404
1101	96-97	0.39748191148493817
1101	98-99	0.4528242048503657
1101	100	0.4323869233535582
1102	1	0.14950255701047155
1102	2	0.30122474557022016
1102	3	0.2708819608583646
1102	4	0.27809016924285856
1102	5	0.2669334027136827
1102	6	0.3651411384240717
1102	7	0.324563455211738
1102	8	0.28567595340525287
1102	9	0.4522968053486949
1102	10-11	0.43480101687835315
1102	12-13	0.42462796864325014
1102	14-15	0.5168895772765012
1102	16-17	0.45671829437528544
1102	18-19	0.46325354176835987
1102	20-21	0.5042272653769615
1102	22-23	0.4839063916667641
1102	24-25	0.47540636455862995
1102	26-27	0.4095900778515258
1102	28-29	0.4439405971844792
1102	30-31	0.45280462239432495
1102	32-33	0.4791314872344046
1102	34-35	0.4325326229559039
1102	36-37	0.46337182615430095
1102	38-39	0.43712231115087974
1102	40-41	0.4009524616495739
1102	42-43	0.43859749021807914
1102	44-45	0.41218773562913213
1102	46-47	0.4275859381832703
1102	48-49	0.43978046164415474
1102	50-51	0.4675907786594422
1102	52-53	0.3919369587301844
1102	54-55	0.41396474761837254
1102	56-57	0.3925786155084623
1102	58-59	0.3674456186483397
1102	60-61	0.3726350013319788
1102	62-63	0.3332252469951058
1102	64-65	0.43673628625366945
1102	66-67	0.49593533016165736
1102	68-69	0.40458484297337804
1102	70-71	0.40518965936693263
1102	72-73	0.3941673255564808
1102	74-75	0.37947805421408276
1102	76-77	0.3837167750381596
1102	78-79	0.4110060943480107
1102	80-81	0.3660412172797578
1102	82-83	0.3659557665477564
1102	84-85	0.30465105429550476
1102	86-87	0.3057394216400553
1102	88-89	0.33599941249950405
1102	90-91	0.3063928765613113
1102	92-93	0.3274408227265724
1102	94-95	0.28786809711279915
1102	96-97	0.30469382269264855
1102	98-99	0.33950996834207103
1102	100	0.39488104496315657
1103	1	0.18190289622872768
1103	2	0.26990714356303513
1103	3	0.32789207135718357
1103	4	0.29206202814973636
1103	5	0.3231530353786951
1103	6	0.3788924921025796
1103	7	0.2866290001595644
1103	8	0.2503668469659388
1103	9	0.35577981650024526
1103	10-11	0.3283993543203252
1103	12-13	0.31320880984345933
1103	14-15	0.3986985692741172
1103	16-17	0.44358826172252463
1103	18-19	0.33903497965879836
1103	20-21	0.354038969845881
1103	22-23	0.4328956424737811
1103	24-25	0.4667551837325732
1103	26-27	0.41771708331188506
1103	28-29	0.3990121686070154
1103	30-31	0.4782436269730681
1103	32-33	0.49909620536987376
1103	34-35	0.5025417736658753
1103	36-37	0.5113920310123987
1103	38-39	0.5294893042218334
1103	40-41	0.47172338339380815
1103	42-43	0.4693702541318743
1103	44-45	0.3740051222453715
1103	46-47	0.4235985200368475
1103	48-49	0.40607675148692124
1103	50-51	0.40635670804194746
1103	52-53	0.3825636875310181
1103	54-55	0.3660596526574622
1103	56-57	0.43615543441574545
1103	58-59	0.40924780736576594
1103	60-61	0.40947458881553445
1103	62-63	0.3753436988057999
1103	64-65	0.3342077029422015
1103	66-67	0.3690407906164097
1103	68-69	0.3818650173791269
1103	70-71	0.4008185415632184
1103	72-73	0.3588082584919974
1103	74-75	0.4063191687862826
1103	76-77	0.38462557525790686
1103	78-79	0.4367031003239106
1103	80-81	0.3860756078714118
1103	82-83	0.37434223830013735
1103	84-85	0.3586922597837088
1103	86-87	0.3702872643613304
1103	88-89	0.27126966581478484
1103	90-91	0.23338770743560389
1103	92-93	0.1849554327399403
1103	94-95	0.30012194273169257
1103	96-97	0.3687174900482262
1103	98-99	0.3426491609567819
1103	100	0.2564894450507218
1104	1	0.1575939198424976
1104	2	0.24174645871669753
1104	3	0.18282477504649464
1104	4	0.2541849311009301
1104	5	0.16718973036485352
1104	6	0.2814307840009036
1104	7	0.19332743300601152
1104	8	0.22332156758778154
1104	9	0.31215234674164094
1104	10-11	0.30632436884096137
1104	12-13	0.36491389770259275
1104	14-15	0.4372739286835028
1104	16-17	0.4049952029424233
1104	18-19	0.4159449821056498
1104	20-21	0.38663403728270396
1104	22-23	0.43774640399290377
1104	24-25	0.550474415114472
1104	26-27	0.4500422230198211
1104	28-29	0.4466462779468259
1104	30-31	0.45992716942274114
1104	32-33	0.5624333048729184
1104	34-35	0.4380093503661513
1104	36-37	0.43781843389829334
1104	38-39	0.489767528469109
1104	40-41	0.42499310055441697
1104	42-43	0.47884419974609926
1104	44-45	0.4173423026034868
1104	46-47	0.41007955333695634
1104	48-49	0.39648490045468776
1104	50-51	0.39554823480593626
1104	52-53	0.38250092625268906
1104	54-55	0.4178983732305923
1104	56-57	0.38542105104136226
1104	58-59	0.29666837157930814
1104	60-61	0.3362705585061221
1104	62-63	0.31616708536395066
1104	64-65	0.4163748129066178
1104	66-67	0.35283073073780713
1104	68-69	0.3021588348213129
1104	70-71	0.2918755622799125
1104	72-73	0.33584694867546006
1104	74-75	0.3167898354811527
1104	76-77	0.2579388042753905
1104	78-79	0.26450947236359923
1104	80-81	0.26869974122468676
1104	82-83	0.21178098750779384
1104	84-85	0.2609983152314541
1104	86-87	0.19328675354467606
1104	88-89	0.18513027967135542
1104	90-91	0.15232781882922808
1104	92-93	0.12871452369631697
1104	94-95	0.19688111893099602
1104	96-97	0.23446751665890986
1104	98-99	0.315247552325566
1104	100	0.45463947132615345
1105	1	0.1034126940022837
1105	2	0.12432420827424195
1105	3	0.10222830664428528
1105	4	0.20009232631707619
1105	5	0.10805059465712219
1105	6	0.26885211252467656
1105	7	0.17859848359078256
1105	8	0.19743350669440218
1105	9	0.25516673516546007
1105	10-11	0.28495987725314365
1105	12-13	0.2951926612408329
1105	14-15	0.35885224697398144
1105	16-17	0.3548626284390579
1105	18-19	0.3622292602757895
1105	20-21	0.40308687724670733
1105	22-23	0.34313839472697794
1105	24-25	0.37329755085855965
1105	26-27	0.3156289138901016
1105	28-29	0.2816294886390125
1105	30-31	0.34711905705773916
1105	32-33	0.34372238091562934
1105	34-35	0.2997028825222898
1105	36-37	0.29559364934808485
1105	38-39	0.31812347477968217
1105	40-41	0.3249129845225056
1105	42-43	0.3258862041790991
1105	44-45	0.2418917635407496
1105	46-47	0.29866178523715803
1105	48-49	0.39240622183682916
1105	50-51	0.35929069876995356
1105	52-53	0.3228829771451913
1105	54-55	0.25368517888623643
1105	56-57	0.2654883575974978
1105	58-59	0.2204232619683708
1105	60-61	0.24955875028598484
1105	62-63	0.31961681417703147
1105	64-65	0.30937852135696886
1105	66-67	0.23076853498720595
1105	68-69	0.2082356098718563
1105	70-71	0.23288656297719967
1105	72-73	0.2318856848418065
1105	74-75	0.19361874035862314
1105	76-77	0.1535003501382377
1105	78-79	0.2167217850420542
1105	80-81	0.18778936600377705
1105	82-83	0.20745770828199994
1105	84-85	0.20425746657284094
1105	86-87	0.15444816981953124
1105	88-89	0.13330903580828135
1105	90-91	0.18800607180631346
1105	92-93	0.20408364825013336
1105	94-95	0.25606894752683473
1105	96-97	0.21324975297393678
1105	98-99	0.2145894517117668
1105	100	0.27805373873191286
1106	1	0.06637668215519099
1106	2	0.1422719325773265
1106	3	0.12886510716211674
1106	4	0.1133105103161185
1106	5	0.06434313954683546
1106	6	0.13509144059310785
1106	7	0.15297555649527084
1106	8	0.23143002751518083
1106	9	0.25245248564826284
1106	10-11	0.2800810771796165
1106	12-13	0.2600270985854465
1106	14-15	0.27168388507688235
1106	16-17	0.2323778826739371
1106	18-19	0.2642809078467181
1106	20-21	0.28615598955918387
1106	22-23	0.30344171925234775
1106	24-25	0.3234371225830941
1106	26-27	0.29895722576423367
1106	28-29	0.24090811610209784
1106	30-31	0.20198336055118205
1106	32-33	0.2677102641168432
1106	34-35	0.26762791266878594
1106	36-37	0.25706805623035933
1106	38-39	0.2428628024932422
1106	40-41	0.21681764706270457
1106	42-43	0.2898439266865225
1106	44-45	0.2841816766890304
1106	46-47	0.30393600304286394
1106	48-49	0.30983063201943395
1106	50-51	0.32315393622358357
1106	52-53	0.2844744035447917
1106	54-55	0.2503137924737757
1106	56-57	0.3260994106276627
1106	58-59	0.3409373955129169
1106	60-61	0.28642433416280966
1106	62-63	0.22478540390287804
1106	64-65	0.3667113422530477
1106	66-67	0.37207044498703823
1106	68-69	0.2568151224602815
1106	70-71	0.14815343135392567
1106	72-73	0.2311593476254572
1106	74-75	0.21423910526788603
1106	76-77	0.24703203643296234
1106	78-79	0.2059258252052132
1106	80-81	0.1687241452334618
1106	82-83	0.2352501541620171
1106	84-85	0.1940407489108651
1106	86-87	0.18198013859350226
1106	88-89	0.22639074257919845
1106	90-91	0.17066979444290808
1106	92-93	0.16028987017520535
1106	94-95	0.18445566447196882
1106	96-97	0.10756418361853548
1106	98-99	0.24764706612474185
1106	100	0.14634636453277494
1107	1	0.07507939079233594
1107	2	0.16204001915870236
1107	3	0.15598099689431777
1107	4	0.1398244691742221
1107	5	0.11014926774517164
1107	6	0.13051990765970345
1107	7	0.16209376487832117
1107	8	0.12071324125639649
1107	9	0.2616502725036298
1107	10-11	0.21600927288534422
1107	12-13	0.2778471524985662
1107	14-15	0.19642388270160893
1107	16-17	0.2120513658150358
1107	18-19	0.2876724042396219
1107	20-21	0.31911692671394576
1107	22-23	0.35280652423157477
1107	24-25	0.3471190545067344
1107	26-27	0.308754699415303
1107	28-29	0.28276311273252475
1107	30-31	0.2519665817496417
1107	32-33	0.2506915188121539
1107	34-35	0.28245704163493457
1107	36-37	0.3205811713149913
1107	38-39	0.266959802609243
1107	40-41	0.3003734030892673
1107	42-43	0.29644082668210103
1107	44-45	0.28029847164792443
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2216	66-67	-0.5066081999053509
2216	68-69	-0.5569990936291092
2216	70-71	-0.5279666336580817
2216	72-73	-0.4895591435649038
2216	74-75	-0.4184412075538617
2216	76-77	-0.5135508295995592
2216	78-79	-0.4813847048196749
2216	80-81	-0.5030518029123314
2216	82-83	-0.4970852275245363
2216	84-85	-0.47280553987887686
2216	86-87	-0.4633109558185282
2216	88-89	-0.40881446507287933
2216	90-91	-0.3398313209275088
2216	92-93	-0.2824968749936829
2216	94-95	-0.4056799040269894
2216	96-97	-0.4748185621892489
2216	98-99	-0.41308255988351306
2216	100	-0.5082234974785855
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	937.0
1	278.0
2	200.0
3	165.0
4	137.0
5	185.0
6	278.0
7	661.0
8	1798.0
9	2152.0
10	2394.0
11	2854.0
12	3703.0
13	4821.0
14	6280.0
15	7456.0
16	8319.0
17	9266.0
18	10324.0
19	11845.0
20	12993.0
21	14705.0
22	16338.0
23	19398.0
24	23494.0
25	29381.0
26	32711.0
27	31299.0
28	32969.0
29	41041.0
30	47794.0
31	54272.0
32	68169.0
33	91474.0
34	136339.0
35	209086.0
36	382593.0
37	773569.0
38	899316.0
39	292089.0
40	443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04889097538075	20.487111170725164	13.232937848284946	24.23106000560914
2	14.968256033761088	20.681255715910016	40.39134384568102	23.959144404647876
3	17.553243531149086	26.51092759184557	30.66034932328329	25.275479553722057
4	12.076911846239707	17.843745837584013	40.067366750110565	30.01197556606571
5	13.008290907494857	38.82111287320285	35.36795493082071	12.802641288481578
6	29.474683411181836	38.93486910752177	18.720402912101964	12.870044569194427
7	26.153349537379956	33.06448515573991	23.611636919621713	17.170528387258425
8	25.137140383712637	35.720604283388916	21.027318340049625	18.114936992848826
9	26.12415797490947	15.52715296618977	21.092122771845105	37.25656628705566
10-11	24.320654782932312	26.676085993752213	28.92886066172627	20.074398561589202
12-13	24.72800550168179	24.66595462575413	28.29006407301523	22.315975799548852
14-15	22.404645817525232	25.867597065610166	25.66300297191018	26.06475414495442
16-17	22.061712413714694	28.527467583670113	26.636772817618464	22.77404718499673
18-19	21.882109459772497	28.492916448226673	27.832734909352485	21.792239182648345
20-21	23.258098326161537	27.659082617906392	27.012996393632584	22.069822662299487
22-23	23.43053232839612	27.402795671199126	26.64708118040805	22.5195908199967
24-25	22.953501091840977	27.639555473126265	27.002509809348062	22.404433625684696
26-27	22.7049673463964	27.876817831049756	26.762895339013525	22.655319483540314
28-29	22.446572290285857	28.061027147052503	26.899645974271795	22.592754588389845
30-31	22.948712477748785	27.635290424053256	26.836075617547365	22.57992148065059
32-33	22.94771552823847	27.961510054870242	26.33739543608597	22.753378980805323
34-35	23.059196418201985	27.89473347300346	26.843976398126312	22.202093710668244
36-37	23.00764375166891	27.552042499698224	26.83323843164701	22.60707531698586
38-39	22.98805218684562	27.593060754746485	26.973044376437734	22.445842681970156
40-41	22.838317861131046	27.20703112795456	27.32153522978625	22.633115781128147
42-43	22.706978512213794	27.395971000077274	26.949528752017798	22.947521735691133
44-45	22.681369093659388	27.80397102697265	26.951819577749752	22.562840301618206
46-47	22.718965253422567	27.504323738099433	26.830981922903653	22.94572908557435
48-49	22.924590865561438	27.271503764062743	27.034888550484897	22.76901681989092
50-51	22.83209352806255	27.33015795648024	26.75708428039093	23.080664235066287
52-53	22.49062296897044	27.619304124308456	26.787549887878022	23.102523018843076
54-55	22.520185854036363	27.535702648173118	26.781093561345827	23.1630179364447
56-57	22.959874613979665	27.388151460045094	26.691550603358493	22.960423322616748
58-59	22.488208645433513	27.32301840995396	27.219779752449764	22.968993192162763
60-61	22.636394855777738	27.62648494353339	26.842624069769194	22.894496130919674
62-63	22.812558112594388	27.325616488886585	26.646297408445037	23.21552799007399
64-65	22.261481244256274	27.38327456928424	26.863749667132442	23.49149451932705
66-67	22.777500352117187	27.12221484452883	26.790203398738722	23.310081404615264
68-69	22.308213375395205	27.173395783915055	27.036119536120296	23.482271304569444
70-71	22.49936093572728	26.918484665429848	27.006604654948735	23.575549743894136
72-73	22.800722921794183	27.02318797414687	27.105345106445313	23.070743997613633
74-75	22.671045709999348	27.38132202659711	26.97817200549301	22.96946025791053
76-77	22.55492882821294	27.18717299732415	26.946963929008838	23.310934245454067
78-79	22.45335112175598	27.172704811776455	26.967569868135595	23.40637419833197
80-81	22.415497169977556	27.20728336746758	27.225971332113485	23.151248130441385
82-83	22.747596752601567	26.686556661944167	27.679608273189267	22.886238312265
84-85	22.834273833368393	27.084876698463802	26.932521790029174	23.14832767813863
86-87	22.23092233641609	27.288418794807406	27.277581335059196	23.203077533717302
88-89	22.69751523029573	26.61689282456748	27.215434910910695	23.470157034226098
90-91	22.643201069173273	26.56391970042906	27.356730339361608	23.43614889103606
92-93	22.900707046027875	26.603077385485875	27.24751061544509	23.248704953041162
94-95	22.251365837041483	26.61305183131522	27.609425094821745	23.52615723682155
96-97	22.462453500776007	26.578634080008463	27.30925506593299	23.649657353282535
98-99	22.232901095560944	26.928016382424868	27.133330213510227	23.70575230850396
100	22.882273350616742	26.478146824325894	26.76243166112466	23.877148163932706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1370.0
1	920.5
2	462.0
3	475.0
4	490.5
5	450.0
6	407.0
7	394.0
8	384.0
9	382.0
10	371.0
11	348.5
12	355.0
13	367.0
14	389.0
15	493.5
16	566.0
17	638.5
18	854.0
19	1094.5
20	1489.5
21	2102.5
22	3093.0
23	4474.0
24	6630.5
25	9330.0
26	12637.0
27	16832.5
28	21167.0
29	27442.0
30	34876.0
31	42493.0
32	52352.5
33	61188.5
34	68593.5
35	75569.5
36	83321.5
37	94732.0
38	100626.0
39	100887.5
40	100167.0
41	98129.0
42	98629.5
43	103966.5
44	111721.0
45	122784.5
46	133203.0
47	139845.5
48	143759.0
49	139538.0
50	131971.5
51	130756.5
52	129746.5
53	124712.0
54	118116.0
55	105963.5
56	95323.5
57	83151.0
58	70748.5
59	63624.0
60	52529.0
61	40393.5
62	31681.0
63	24261.0
64	17105.5
65	11328.5
66	8075.0
67	5829.0
68	4084.5
69	3021.0
70	2373.5
71	1773.5
72	1217.0
73	908.0
74	761.0
75	592.0
76	416.5
77	302.0
78	185.5
79	119.0
80	81.0
81	47.0
82	25.0
83	25.5
84	31.0
85	21.5
86	9.0
87	10.0
88	14.0
89	11.0
90	11.0
91	12.0
92	8.0
93	8.0
94	6.5
95	2.5
96	4.0
97	5.5
98	5.0
99	4.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09642073795060555
2	0.06441246391836093
3	0.07951817649685125
4	0.08158912096325718
5	0.08232004253963573
6	0.09163929263846243
7	0.08722330811450861
8	0.08972062350046871
9	0.07455400079061351
10-11	0.08171094122598695
12-13	0.07149326668952827
14-15	0.08038614586880079
16-17	0.08207640201417622
18-19	0.08180230642303427
20-21	0.07263533165261978
22-23	0.0768990408481614
24-25	0.0791679432415032
26-27	0.0736403488201403
28-29	0.08195458175144646
30-31	0.08545691430492708
32-33	0.08761922396838033
34-35	0.08950743804069163
36-37	0.08947698297500918
38-39	0.09037540741264118
40-41	0.08702535018757275
42-43	0.09241589681336466
44-45	0.0879694572237284
46-47	0.08527418391083244
48-49	0.09424320075431107
50-51	0.09046677260968848
52-53	0.09654255821333531
54-55	0.08792377462520473
56-57	0.09430411088567595
58-59	0.09935965178896101
60-61	0.09820235929302829
62-63	0.09916169386202514
64-65	0.10103468040149521
66-67	0.10257266121845846
68-69	0.09986216037272129
70-71	0.1017046918465089
72-73	0.09826326942439316
74-75	0.10225288302879282
76-77	0.09923783152623125
78-79	0.10628817923171614
80-81	0.10171991937935013
82-83	0.10613590390330394
84-85	0.10220720043026917
86-87	0.0985830476140588
88-89	0.09812622162882217
90-91	0.09850690994985269
92-93	0.09278135760155394
94-95	0.09223316641927001
96-97	0.09016222195286408
98-99	0.10432382749519875
100	0.24175231138720996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3283526.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.948268004736875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.99815906263143	35.474364818721995
2	15.880705834334464	16.817117372414593
3	6.965100877661756	11.063700838513881
4	3.5553728939485634	7.530033473822619
5	2.077686737822792	5.500495711206434
6	1.2946554109635775	4.112985700408935
7	0.8146510284206969	3.019405268820751
8	0.5577098180174874	2.3623815130610364
9	0.4045478345364725	1.9278096447395785
>10	1.4350601249563595	11.441037966950748
>50	0.013679818260010006	0.48735171356840856
>100	0.0025717055946634284	0.2085150838874316
>500	0.0	0.0
>1k	9.885285172036281E-5	0.05480089388360632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0455065682440158E-5
2	0.0	0.0	0.0	0.0	3.0455065682440158E-5
3	0.0	0.0	0.0	0.0	3.0455065682440158E-5
4	0.0	0.0	0.0	0.0	3.0455065682440158E-5
5	0.0	0.0	0.0	0.0	3.0455065682440158E-5
6	0.0	0.0	0.0	0.0	3.0455065682440158E-5
7	0.0	0.0	0.0	0.0	3.0455065682440158E-5
8	0.0	0.0	0.0	0.0	3.0455065682440158E-5
9	0.0	0.0	0.0	0.0	3.0455065682440158E-5
10-11	0.0	0.0	0.0	0.0	6.0910131364880316E-5
12-13	0.0	0.0	0.0	0.0	3.350057225068417E-4
14-15	0.0	0.0	0.0	0.0	4.8728105091904253E-4
16-17	0.0	0.0	0.0	0.0	6.547839121724634E-4
18-19	0.0	0.0	0.0	0.0	7.309215763785637E-4
20-21	0.0	0.0	0.0	1.5227532841220079E-5	8.527418391083244E-4
22-23	0.0	0.0	0.0	1.6750286125342089E-4	8.831969047907646E-4
24-25	0.0	0.0	0.0	4.568259852366024E-4	8.831969047907646E-4
26-27	0.0	0.0	0.0	0.0011877475616151661	9.288795033144248E-4
28-29	0.0	0.0	0.0	0.004263709195541622	9.441070361556449E-4
30-31	0.0	0.0	0.0	0.012730217455259986	9.59334568996865E-4
32-33	0.0	0.0	0.0	0.03583038477539084	0.0010050171675205252
34-35	0.0	0.0	0.0	0.0725744215212549	0.0010050171675205252
36-37	0.0	0.0	0.0	0.1305151839820973	0.0010050171675205252
38-39	0.0	0.0	0.0	0.21866737159992033	0.0010659272988854055
40-41	0.0	0.0	0.0	0.3158647137254281	0.0010963823645678457
42-43	0.0	0.0	0.0	0.4157116465653081	0.0011268374302502858
44-45	0.0	0.0	0.0	0.5439274730883812	0.0011725200287739461
46-47	0.0	0.0	0.0	0.7008928816156778	0.0012182026272976063
48-49	0.0	0.0	0.0	0.8747608515967287	0.0012182026272976063
50-51	0.0	0.0	0.0	1.0554659838234874	0.0012486576929800464
52-53	0.0	0.0	0.0	1.234663590298965	0.0013400228900273669
54-55	0.0	0.0	0.0	1.4337026720665529	0.001370477955709807
56-57	0.0	0.0	0.0	1.6730947158633738	0.0014009330213922472
58-59	0.0	0.0	0.0	1.94787554598319	0.0014009330213922472
60-61	0.0	0.0	0.0	2.2335136070187964	0.0014313880870746873
62-63	0.0	0.0	0.0	2.5240853886949575	0.0015227532841220078
64-65	0.0	0.0	0.0	2.8354427526993846	0.0015227532841220078
66-67	0.0	0.0	0.0	3.1890717478710386	0.001553208349804448
68-69	0.0	0.0	0.0	3.5853530625309498	0.0015988909483281083
70-71	0.0	0.0	0.0	4.008983026173692	0.0016293460140105486
72-73	0.0	0.0	0.0	4.448693264496763	0.0017207112110578689
74-75	0.0	0.0	0.0	4.885875732368191	0.0018577590066288497
76-77	0.0	0.0	0.0	5.374664309038515	0.00197957926935861
78-79	0.0	0.0	0.0	5.914358527997038	0.00199480680219983
80-81	0.0	0.0	0.0	6.49574573187482	0.0020100343350410504
82-83	0.0	0.0	0.0	7.095101424505242	0.0021013995320883706
84-85	0.0	0.0	0.0	7.694929170653742	0.0021013995320883706
86-87	0.0	0.0	0.0	8.312405627365216	0.0022384473276593512
88	0.0	0.0	0.0	8.79831620032855	0.0023754951232303322
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3310	0.0	39.786842	1
GTATCAA	5280	0.0	34.206352	1
CAACGCA	5425	0.0	31.10754	5
ATCAACG	5525	0.0	30.630524	3
TATCAAC	6410	0.0	27.721159	2
AACGCAG	6565	0.0	26.91155	6
TCAACGC	6750	0.0	25.21017	4
GTACATG	11390	0.0	25.065388	1
ACGCAGA	6820	0.0	24.940765	7
GTACTAG	1815	0.0	24.877346	1
TACATGG	11315	0.0	24.677975	2
CGCAGAG	6955	0.0	24.389093	8
ACATGGG	11105	0.0	24.171396	3
CGTTAAC	505	0.0	23.284027	1
CATGGGG	9330	0.0	21.816105	4
TGATCGC	965	0.0	20.45036	9
GCAGAGT	8110	0.0	20.220112	9
GTGATCG	995	0.0	19.834068	8
ATGGGGG	6780	0.0	19.413298	5
AGAGTAC	6820	0.0	19.361279	10-11
>>END_MODULE