FastQCFastQC Report
Mon 16 Jan 2017
ERR1146617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283526
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96760.29468321554329097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90860.2767147267906513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50110.15261033413470762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61300.057.3169861
GTATCAA116150.044.544771
CAACGCA142900.035.3893435
ATCAACG143050.035.3446733
TATCAAC149450.034.3671342
AACGCAG157350.032.796046
TCAACGC159100.031.9269184
ACGCAGA168000.030.1570427
CGCAGAG170350.029.6858398
GTGGTAT18650.027.2375581
CGCGTTG700.0088402226.8559887
GCAGAGT188450.026.6346959
GAGTACT125650.023.88201712-13
CGTCGAA1000.00175265123.51546594
GTACATG108250.022.9853441
CAGAGTA183450.022.87738210-11
AGAGTAC174850.022.71243310-11
TGGTATC22950.022.338832
ACATGGG105350.022.277653
TACATGG108700.022.197522
Produced by FastQC (version 0.11.5)