##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3283526
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28595296641476	33.0	31.0	34.0	30.0	34.0
2	32.38960586881298	34.0	31.0	34.0	30.0	34.0
3	32.5320353790407	34.0	31.0	34.0	31.0	34.0
4	36.0596057409017	37.0	35.0	37.0	35.0	37.0
5	36.082648652698346	37.0	35.0	37.0	35.0	37.0
6	36.14959193257492	37.0	36.0	37.0	35.0	37.0
7	36.123924403217764	37.0	36.0	37.0	35.0	37.0
8	36.12843175293876	37.0	36.0	37.0	35.0	37.0
9	37.91916981927355	39.0	38.0	39.0	35.0	39.0
10-11	37.86635555801903	39.0	38.0	39.0	35.0	39.0
12-13	37.83277565032225	39.0	38.0	39.0	35.0	39.0
14-15	39.19011087471212	40.0	39.0	41.0	36.0	41.0
16-17	39.136599801554794	40.0	39.0	41.0	36.0	41.0
18-19	39.236795444896735	40.5	39.0	41.0	36.0	41.0
20-21	39.29085242510642	41.0	39.0	41.0	36.0	41.0
22-23	39.25453293197617	41.0	39.0	41.0	36.0	41.0
24-25	39.24003175245148	41.0	39.0	41.0	36.0	41.0
26-27	39.158789819237	41.0	39.0	41.0	35.5	41.0
28-29	39.10257357487043	40.5	39.0	41.0	35.0	41.0
30-31	38.96068997169506	40.0	38.5	41.0	35.0	41.0
32-33	38.85128989385191	40.0	38.0	41.0	35.0	41.0
34-35	38.77370774588049	40.0	38.0	41.0	35.0	41.0
36-37	38.70316361131296	40.0	38.0	41.0	35.0	41.0
38-39	38.61096470684258	40.0	38.0	41.0	35.0	41.0
40-41	38.50536222341471	40.0	38.0	41.0	35.0	41.0
42-43	38.38689826119848	40.0	38.0	41.0	34.0	41.0
44-45	38.221820841376015	40.0	38.0	41.0	34.0	41.0
46-47	37.959918544881326	40.0	37.5	41.0	33.0	41.0
48-49	37.65320268516223	40.0	37.0	41.0	33.0	41.0
50-51	37.52889957320271	40.0	37.0	41.0	33.0	41.0
52-53	37.41590823401429	40.0	36.5	41.0	32.5	41.0
54-55	37.25271110994705	40.0	36.0	41.0	32.0	41.0
56-57	37.07713156527465	39.5	35.5	41.0	32.0	41.0
58-59	36.87904374748365	39.0	35.0	41.0	32.0	41.0
60-61	36.67514251447986	39.0	35.0	41.0	31.0	41.0
62-63	36.433198793004834	38.5	35.0	41.0	31.0	41.0
64-65	36.161426771099116	37.5	35.0	40.0	31.0	41.0
66-67	35.89529015454728	37.0	35.0	40.0	31.0	41.0
68-69	35.61292251073998	37.0	35.0	40.0	31.0	41.0
70-71	35.327988113996966	36.0	35.0	39.0	30.0	41.0
72-73	35.02286520648839	36.0	35.0	39.0	30.0	41.0
74-75	34.716201272656285	35.0	35.0	38.5	30.0	40.5
76-77	34.430677418116986	35.0	34.0	37.0	30.0	39.5
78-79	34.16001731065933	35.0	34.0	37.0	29.5	39.0
80-81	33.921194015214134	35.0	34.0	36.5	29.0	39.0
82-83	33.67718650621313	35.0	34.0	36.0	29.0	37.5
84-85	33.47643280424763	35.0	34.0	36.0	29.0	37.0
86-87	33.29711566163935	35.0	34.0	35.0	29.0	37.0
88-89	33.11866664067834	35.0	34.0	35.0	29.0	36.0
90-91	33.0361611267887	35.0	34.0	35.0	29.0	36.0
92-93	32.94070368256563	35.0	34.0	35.0	29.0	36.0
94-95	32.87867402298627	35.0	34.0	35.0	29.0	36.0
96-97	32.82216906459702	35.0	34.0	35.0	29.0	35.5
98-99	32.759967486171874	35.0	34.0	35.0	29.0	35.0
100	32.02142696601154	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35496331656388236
1101	2	0.32475663199118543
1101	3	0.2878254904445541
1101	4	0.22506477322126983
1101	5	0.20207575880067452
1101	6	0.2518694125565304
1101	7	0.24349945571036358
1101	8	0.2530275942543554
1101	9	0.28287369955361896
1101	10-11	0.3182270498710267
1101	12-13	0.36808087912075393
1101	14-15	0.4835253732161746
1101	16-17	0.5199895466239326
1101	18-19	0.43840363139162264
1101	20-21	0.4225735854775152
1101	22-23	0.3627058425448837
1101	24-25	0.38773106475758823
1101	26-27	0.348804110450736
1101	28-29	0.4308010238150999
1101	30-31	0.41122260222408613
1101	32-33	0.40904273049439865
1101	34-35	0.3512813120025484
1101	36-37	0.3771537411291419
1101	38-39	0.3840402685411206
1101	40-41	0.3491392882927258
1101	42-43	0.3729916980379002
1101	44-45	0.4234328143243573
1101	46-47	0.4129266413762025
1101	48-49	0.48571805609307717
1101	50-51	0.46272075412481684
1101	52-53	0.4004517891940296
1101	54-55	0.44967376852764573
1101	56-57	0.3645499912878378
1101	58-59	0.34931792479839885
1101	60-61	0.35220388851136164
1101	62-63	0.3143321006169728
1101	64-65	0.2806229598447487
1101	66-67	0.29746283338784707
1101	68-69	0.3325578705097101
1101	70-71	0.32189194760130846
1101	72-73	0.35083298417737296
1101	74-75	0.30074892642626594
1101	76-77	0.2792309613756103
1101	78-79	0.3241560023464487
1101	80-81	0.2925792528273874
1101	82-83	0.25175422063050235
1101	84-85	0.24161246643781453
1101	86-87	0.24296810669302715
1101	88-89	0.40382664812062075
1101	90-91	0.34190373187296075
1101	92-93	0.3296549764483103
1101	94-95	0.3049956139421539
1101	96-97	0.25885656418263636
1101	98-99	0.2832529956711909
1101	100	0.29686727712830674
1102	1	0.24375433819636072
1102	2	0.2721771307328922
1102	3	0.27374388567574925
1102	4	0.19337039868200634
1102	5	0.1458991592429868
1102	6	0.14254342562244204
1102	7	0.18358086347365088
1102	8	0.2181128150276308
1102	9	0.22443407856681574
1102	10-11	0.24046126718717886
1102	12-13	0.24940984020263102
1102	14-15	0.3714658553077399
1102	16-17	0.46244137559772724
1102	18-19	0.3689054076187759
1102	20-21	0.3031158749358198
1102	22-23	0.39023283547810195
1102	24-25	0.31408801968780864
1102	26-27	0.3528895123419886
1102	28-29	0.3062528654989265
1102	30-31	0.3541562208976785
1102	32-33	0.40984370090544786
1102	34-35	0.35951510988759594
1102	36-37	0.3228716514725605
1102	38-39	0.3129274733505909
1102	40-41	0.36382477851871187
1102	42-43	0.4406236122960152
1102	44-45	0.3779830062458558
1102	46-47	0.39728440339354165
1102	48-49	0.49564570422851517
1102	50-51	0.4132169220497701
1102	52-53	0.33859015061653963
1102	54-55	0.3511417773738259
1102	56-57	0.32202730387840006
1102	58-59	0.2855192771459514
1102	60-61	0.316763072781427
1102	62-63	0.3516455808782908
1102	64-65	0.3124985317962441
1102	66-67	0.2495845351342041
1102	68-69	0.3080359266372099
1102	70-71	0.2782035118251045
1102	72-73	0.24328306552158807
1102	74-75	0.281854985299141
1102	76-77	0.23969695885902098
1102	78-79	0.267087952828156
1102	80-81	0.21778062741748982
1102	82-83	0.23846627800359244
1102	84-85	0.2629030432191257
1102	86-87	0.24662311508164692
1102	88-89	0.2731139647783962
1102	90-91	0.21499174567605195
1102	92-93	0.2297272647627011
1102	94-95	0.24705136650322856
1102	96-97	0.2379066162937704
1102	98-99	0.1881739233765245
1102	100	0.3152634615763148
1103	1	0.16004623592449008
1103	2	0.21373476424611226
1103	3	0.2058516622577926
1103	4	0.1720639910416537
1103	5	0.11604187752556072
1103	6	0.15297923390338042
1103	7	0.19089860446594997
1103	8	0.178000446796851
1103	9	0.22796827879923143
1103	10-11	0.24935834475274987
1103	12-13	0.26550135059200386
1103	14-15	0.41556579588441167
1103	16-17	0.44579762554086955
1103	18-19	0.34069590133511696
1103	20-21	0.3803376283060089
1103	22-23	0.3881549977492966
1103	24-25	0.3816514384563803
1103	26-27	0.37430314000713594
1103	28-29	0.3633480170001562
1103	30-31	0.39745496677804226
1103	32-33	0.3448938344910033
1103	34-35	0.3968722164601388
1103	36-37	0.36236755596059567
1103	38-39	0.3336186508438814
1103	40-41	0.3434363459659906
1103	42-43	0.35800102282549773
1103	44-45	0.3423203718436838
1103	46-47	0.3212364677156927
1103	48-49	0.41795659799377205
1103	50-51	0.4054194922360992
1103	52-53	0.34945846268396963
1103	54-55	0.32031082886364004
1103	56-57	0.24421997805735174
1103	58-59	0.276002523348879
1103	60-61	0.2578961694802615
1103	62-63	0.24569687923518302
1103	64-65	0.27992906146985064
1103	66-67	0.3179642344462792
1103	68-69	0.26899740344861556
1103	70-71	0.22330629373242772
1103	72-73	0.17572278214204573
1103	74-75	0.2350016848575791
1103	76-77	0.21196822079297561
1103	78-79	0.18937147618919425
1103	80-81	0.15053244374578156
1103	82-83	0.16164585442898272
1103	84-85	0.17842774723197152
1103	86-87	0.2283530233160036
1103	88-89	0.23390795527590313
1103	90-91	0.17833647794749652
1103	92-93	0.1797912858086761
1103	94-95	0.16121680227270474
1103	96-97	0.18082624224082622
1103	98-99	0.1577739366582378
1103	100	0.1833685419293971
1104	1	0.17646015902846557
1104	2	0.19423765020491857
1104	3	0.22936978737479308
1104	4	0.19583562636539398
1104	5	0.14189316425748189
1104	6	0.16527674681639581
1104	7	0.15034697966618182
1104	8	0.13611131161226098
1104	9	0.15183125569435418
1104	10-11	0.21547982642967867
1104	12-13	0.2553044423811883
1104	14-15	0.3674575258263246
1104	16-17	0.44010725320477917
1104	18-19	0.3161177860498938
1104	20-21	0.31838581269814625
1104	22-23	0.34774267537837744
1104	24-25	0.28941940138256683
1104	26-27	0.28078627159477065
1104	28-29	0.28886553994413333
1104	30-31	0.32349028259373114
1104	32-33	0.2773330439315984
1104	34-35	0.3042715986189819
1104	36-37	0.3204717896747198
1104	38-39	0.29962084665049815
1104	40-41	0.30019108894129687
1104	42-43	0.35792642909877515
1104	44-45	0.29844439601797035
1104	46-47	0.323614715889164
1104	48-49	0.32234440017909094
1104	50-51	0.29760801548096794
1104	52-53	0.2685295937925787
1104	54-55	0.26224417303352965
1104	56-57	0.32159775663053125
1104	58-59	0.18987031062728477
1104	60-61	0.19072597297238758
1104	62-63	0.25465894820188595
1104	64-65	0.1965044334019055
1104	66-67	0.1707952980941556
1104	68-69	0.13808462422193912
1104	70-71	0.1530371789913545
1104	72-73	0.09743543340079697
1104	74-75	0.08788638893814493
1104	76-77	0.09138876804666296
1104	78-79	0.13787360408247906
1104	80-81	0.13004793653743008
1104	82-83	0.09000634495694015
1104	84-85	0.13285061044093283
1104	86-87	0.15379188512912378
1104	88-89	0.1566856501443752
1104	90-91	0.14140610002451837
1104	92-93	0.12297310118924543
1104	94-95	0.13089151889912642
1104	96-97	0.13928350198284534
1104	98-99	0.24331238232907992
1104	100	0.30065418615715345
1105	1	0.16171952332008743
1105	2	0.10971718425943067
1105	3	0.13528506434643361
1105	4	0.1446532331943473
1105	5	0.06860095369125929
1105	6	0.14184702753414768
1105	7	0.14104923499091626
1105	8	0.11893700535607366
1105	9	0.15239739167346755
1105	10-11	0.14405758507656685
1105	12-13	0.16053493503635252
1105	14-15	0.2608275671049043
1105	16-17	0.3250289160863815
1105	18-19	0.29321768308067675
1105	20-21	0.22578800816309297
1105	22-23	0.2594644169476936
1105	24-25	0.23033285399399261
1105	26-27	0.25881896205212485
1105	28-29	0.2921774678838531
1105	30-31	0.31715257858675727
1105	32-33	0.2671010450430984
1105	34-35	0.2388111521766163
1105	36-37	0.24748585737471984
1105	38-39	0.19146274279051312
1105	40-41	0.23617828206248248
1105	42-43	0.20969618834815407
1105	44-45	0.19424697167697502
1105	46-47	0.2500748208320758
1105	48-49	0.39262317020973114
1105	50-51	0.4136593988134578
1105	52-53	0.3237228201573714
1105	54-55	0.32405622367932807
1105	56-57	0.34852200218292495
1105	58-59	0.25697657668782625
1105	60-61	0.28794732954326463
1105	62-63	0.29650626548345116
1105	64-65	0.29525296125359546
1105	66-67	0.28380434977744073
1105	68-69	0.20282003356032874
1105	70-71	0.2736165139893387
1105	72-73	0.30414631970224804
1105	74-75	0.27911196296237506
1105	76-77	0.24437695320430208
1105	78-79	0.3160981769809368
1105	80-81	0.3046093434632482
1105	82-83	0.23685516847183408
1105	84-85	0.1865562690739182
1105	86-87	0.180422025637327
1105	88-89	0.26530503796440286
1105	90-91	0.26751159089428
1105	92-93	0.26202814103301364
1105	94-95	0.2387279250783152
1105	96-97	0.2512456565717258
1105	98-99	0.2906397595483412
1105	100	0.25394634478430334
1106	1	0.0764531943509894
1106	2	0.0018557083975636601
1106	3	0.038004495015876216
1106	4	0.09381885475872309
1106	5	0.07332715818494506
1106	6	0.08366109088473195
1106	7	0.10930899951109296
1106	8	0.08038905521153339
1106	9	0.07242204828018117
1106	10-11	0.07347095088791633
1106	12-13	0.08912295394117109
1106	14-15	0.2518342756324472
1106	16-17	0.30919446312734067
1106	18-19	0.1695186491171583
1106	20-21	0.13980146354449374
1106	22-23	0.16321865857250373
1106	24-25	0.13561624527815752
1106	26-27	0.12605974297242284
1106	28-29	0.1301922916572522
1106	30-31	0.14405711337251859
1106	32-33	0.16479857292373623
1106	34-35	0.16158048869812802
1106	36-37	0.14904565035575246
1106	38-39	0.1304479325023138
1106	40-41	0.10282083507880202
1106	42-43	0.1841689883634885
1106	44-45	0.14304751718843534
1106	46-47	0.25125344424960616
1106	48-49	0.3558009696111242
1106	50-51	0.30192625370499826
1106	52-53	0.2408566777097505
1106	54-55	0.28603676880752005
1106	56-57	0.256839383955068
1106	58-59	0.2957191749150212
1106	60-61	0.23639726009453454
1106	62-63	0.23679455047237497
1106	64-65	0.2595403611881508
1106	66-67	0.2683862520836158
1106	68-69	0.2177576326143793
1106	70-71	0.21108666026106704
1106	72-73	0.18598260167900804
1106	74-75	0.22014264319088284
1106	76-77	0.24166536523791393
1106	78-79	0.23573823949543993
1106	80-81	0.2429999427238343
1106	82-83	0.17224149789517185
1106	84-85	0.16349650412789174
1106	86-87	0.18163746717441143
1106	88-89	0.26250224752354967
1106	90-91	0.2399209792841006
1106	92-93	0.23133132218951857
1106	94-95	0.2187964582373354
1106	96-97	0.23303559207703017
1106	98-99	0.1558154431942853
1106	100	0.24834945830192368
1107	1	0.10276881120326209
1107	2	0.11845180742977846
1107	3	0.12077352894358029
1107	4	0.078329880433742
1107	5	0.11534787137377833
1107	6	0.09146340622124427
1107	7	0.07793384898521794
1107	8	0.13257803285084435
1107	9	0.1490286703138537
1107	10-11	0.16137702550455657
1107	12-13	0.16034680870165374
1107	14-15	0.25531889782630657
1107	16-17	0.26876436996700903
1107	18-19	0.21418107413041554
1107	20-21	0.1595329543858952
1107	22-23	0.20791250068329248
1107	24-25	0.23085743537162529
1107	26-27	0.19947114261447751
1107	28-29	0.25116149530632725
1107	30-31	0.29581766192194436
1107	32-33	0.24508531090375385
1107	34-35	0.23492836365946346
1107	36-37	0.22172306374122286
1107	38-39	0.27400128218634023
1107	40-41	0.2405242176299538
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2216	44-45	-0.5794615967962784
2216	46-47	-0.6433441860064519
2216	48-49	-0.49561227809715547
2216	50-51	-0.5256414995170005
2216	52-53	-0.5627010004851059
2216	54-55	-0.5135081794872107
2216	56-57	-0.5379546502431438
2216	58-59	-0.5876066443723076
2216	60-61	-0.5933896323874279
2216	62-63	-0.5464814764562433
2216	64-65	-0.5481714917405114
2216	66-67	-0.5252619238243739
2216	68-69	-0.5104030692218018
2216	70-71	-0.5092060125053237
2216	72-73	-0.5099627408535667
2216	74-75	-0.5468606851132947
2216	76-77	-0.5444328132602081
2216	78-79	-0.48661830023376496
2216	80-81	-0.5065486749075419
2216	82-83	-0.42954070700921676
2216	84-85	-0.41247684137900364
2216	86-87	-0.4239761592310032
2216	88-89	-0.3439591035693681
2216	90-91	-0.3792036303092772
2216	92-93	-0.3756423195623526
2216	94-95	-0.30898372529425444
2216	96-97	-0.3859104071588888
2216	98-99	-0.3867689623408026
2216	100	-0.519601826937194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	21.0
8	150.0
9	239.0
10	260.0
11	176.0
12	173.0
13	233.0
14	383.0
15	815.0
16	1461.0
17	2586.0
18	4582.0
19	6965.0
20	10002.0
21	15097.0
22	21981.0
23	25756.0
24	23932.0
25	20502.0
26	18816.0
27	21278.0
28	26186.0
29	32791.0
30	39749.0
31	50481.0
32	64906.0
33	84743.0
34	123796.0
35	193494.0
36	350027.0
37	785541.0
38	1001540.0
39	354105.0
40	758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72353805408488	20.431426629209927	13.611489441535566	24.23354587516964
2	15.167856285732734	20.90132679377865	39.8695378332974	24.06127908719122
3	17.313797388168794	26.602670305038494	31.338624403079624	24.744907903713088
4	12.213836520147268	18.212966904323064	39.33280904497962	30.240387530550056
5	12.59796452460652	38.44774672076003	35.47633760616472	13.477951148468733
6	28.96690795426754	39.248123186117134	18.27605100511458	13.508917854500742
7	25.29777440471006	33.686591791872516	23.577641839900156	17.43799196351727
8	25.506393455304288	34.916769046819965	20.839838051550867	18.73699944632488
9	26.30254793170512	14.530720938405848	21.19023878598799	37.976492343901036
10-11	24.38551147875141	26.23188169659818	28.803920544500443	20.578686280149967
12-13	25.328610158713527	24.36383936049235	28.52345314153139	21.784097339262733
14-15	22.585243387098224	25.212039684796018	26.719246346418306	25.48347058168745
16-17	21.397020967313843	28.016679074607687	27.657810660555736	22.92848929752273
18-19	21.152689445263178	27.053062855392124	30.217364225780802	21.576883473563896
20-21	22.47427415089889	26.246845417100843	29.763791349914033	21.515089082086234
22-23	22.538499771282456	26.029396447599318	29.500299373295658	21.93180440782257
24-25	22.081963109169838	26.243845792602222	29.841457019070354	21.83273407915759
26-27	21.944763758466728	26.483711279484222	29.571713479565876	21.999811482483175
28-29	21.626724080565236	26.68914859957689	29.754862570733803	21.929264749124076
30-31	22.179962357470032	26.272491107104575	29.56368470421986	21.983861831205537
32-33	22.259751437178927	26.544929277031088	29.11709582045588	22.078223465334112
34-35	22.359014637781637	26.51739815475662	29.480678301830338	21.642908905631405
36-37	22.24634603650621	26.26899122238656	29.414103477560005	22.070559263547224
38-39	22.346728649335457	26.25306513768774	29.53529596556843	21.864910247408368
40-41	22.214391839943058	25.950824217951148	29.70676318137333	22.12802076073247
42-43	22.247181840497078	26.16183030071941	29.12917394288944	22.461813915894073
44-45	22.2385546916757	26.61369383406218	28.95315892428431	22.194592549977813
46-47	22.461341862375996	26.42592140278469	28.487165930770765	22.625570804068555
48-49	22.867775160502077	26.39698592168263	28.27230313037104	22.462935787444255
50-51	22.623515087134987	26.641619405480572	27.855512640984117	22.879352866400325
52-53	22.225257585883913	26.97336833848711	27.862765422375674	22.938608653253304
54-55	22.365787882802422	26.858001257798424	27.740454969750406	23.035755889648748
56-57	22.768292283667922	26.690756649299647	27.664710687988283	22.87624037904415
58-59	22.17142616981082	26.705154041372303	28.236035256270668	22.887384532546207
60-61	22.39889130787997	27.0395523568768	27.80210558309808	22.759450752145153
62-63	22.49282781892603	26.703933583471397	27.674949444498587	23.128289153103985
64-65	21.977046241154106	26.703567168654196	27.871431662628567	23.447954927563135
66-67	22.409203261897105	26.5207913048563	27.78784329347328	23.282162139773313
68-69	22.049433546974935	26.41029268376622	28.058341532259355	23.48193223699949
70-71	22.221452362979733	26.17284037097199	27.937246082867347	23.668461183180934
72-73	22.44150453861097	26.202165808800743	27.963657610763835	23.392672041824447
74-75	22.306449360649523	26.64399132680433	27.747023366736723	23.30253594580943
76-77	22.244634606230182	26.479356935472133	27.564252697582354	23.71175576071533
78-79	22.015871253807124	26.529842467610997	27.646948735999434	23.807337542582445
80-81	21.936301942048875	26.58987035444897	27.830333629664285	23.643494073837875
82-83	22.263110063992535	26.055806777385648	28.257674414071783	23.423408744550038
84-85	22.384291434648553	26.408676330315018	27.477122029244335	23.7299102057921
86-87	21.669068556180154	26.66305977172101	27.77244644933526	23.895425222763578
88-89	22.05292420404163	26.131253414774243	27.639768955689707	24.176053425494423
90-91	21.903073388648878	26.020020249576252	27.793738772547982	24.283167589226885
92-93	22.030965839571937	26.035462231631495	27.728187557169946	24.20538437162662
94-95	21.37978670464215	26.00524680510744	28.104060213392813	24.510906276857593
96-97	21.601107593707898	25.937802315171748	27.760925069768994	24.70016502135136
98-99	21.346800577733212	26.300230925781687	27.522942206121627	24.830026290363474
100	21.786461763718926	25.858197707856036	27.26982630165561	25.085514226769433
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	143.0
1	94.5
2	166.0
3	279.0
4	189.0
5	162.5
6	349.5
7	894.5
8	1727.0
9	2147.5
10	1684.5
11	1222.0
12	1283.0
13	1490.0
14	1795.0
15	2271.5
16	2779.5
17	3198.0
18	3750.0
19	4318.5
20	5080.5
21	6016.5
22	6937.5
23	8227.0
24	10244.0
25	12684.0
26	15766.0
27	19534.5
28	23275.0
29	29134.5
30	36138.0
31	42816.0
32	51820.5
33	59863.5
34	66271.5
35	72848.5
36	80245.0
37	91437.0
38	97627.0
39	98157.0
40	97903.0
41	95970.5
42	96193.0
43	100656.5
44	108725.5
45	120483.0
46	131161.0
47	137513.5
48	141466.5
49	138281.5
50	131426.0
51	129338.0
52	127719.5
53	123552.0
54	117929.5
55	105783.5
56	94927.5
57	83623.0
58	71268.0
59	64333.5
60	53094.5
61	40405.0
62	30726.5
63	22955.0
64	16283.0
65	10608.5
66	7472.5
67	5403.0
68	3812.5
69	2817.5
70	2183.0
71	1595.0
72	1102.5
73	838.0
74	667.5
75	518.0
76	344.5
77	212.0
78	120.0
79	61.0
80	26.5
81	13.0
82	6.5
83	2.0
84	1.0
85	2.5
86	2.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0028627761741493746
2	0.0037155180132576993
3	1.8273039409464093E-4
4	0.04924584120850574
5	0.0020404894007234908
6	0.005146906100332386
7	0.0
8	3.6546078818928187E-4
9	0.0
10-11	7.613766420610039E-5
12-13	0.0
14-15	4.5682598523660234E-5
16-17	3.1977818966562164E-4
18-19	1.5227532841220078E-4
20-21	2.893231239831815E-4
22-23	0.0
24-25	0.0
26-27	3.502332553480618E-4
28-29	1.5227532841220077E-5
30-31	1.8273039409464093E-4
32-33	3.0455065682440157E-4
34-35	7.613766420610039E-5
36-37	7.613766420610039E-5
38-39	8.375143062671043E-4
40-41	5.938737808075831E-4
42-43	0.0
44-45	5.78646247966363E-4
46-47	0.0
48-49	4.5682598523660234E-5
50-51	0.0
52-53	2.893231239831815E-4
54-55	3.3500572250684177E-4
56-57	1.0659272988854054E-4
58-59	7.61376642061004E-4
60-61	5.329636494427028E-4
62-63	1.8273039409464093E-4
64-65	3.502332553480618E-4
66-67	0.001157292495932726
68-69	0.0020557169335647107
70-71	0.0029693689040379153
72-73	0.0026648182472135138
74-75	0.0023298125247066723
76-77	0.0037459730789401392
78-79	0.0012943402915037065
80-81	0.0010659272988854055
82-83	5.481911822839228E-4
84-85	5.481911822839228E-4
86-87	0.0
88-89	0.0
90-91	1.5227532841220077E-5
92-93	1.370477955709807E-4
94-95	1.2182026272976062E-4
96-97	3.502332553480618E-4
98-99	1.0659272988854054E-4
100	0.07592447874632331
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3283526.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.41512524023719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.7750983605187	33.1605982153447
2	16.4372092741005	16.5736792830753
3	7.12698756289866	10.77923911707446
4	3.724301227008684	7.510444511680473
5	2.1538139865197303	5.429240093728336
6	1.3361262548403585	4.041658348472745
7	0.8800540501942936	3.1057624611106305
8	0.6035064899310022	2.4340684218533934
9	0.4308854031706012	1.9550827408532356
>10	1.4993613912418597	11.428862786066771
>50	0.021392031594875377	0.7304741273303402
>100	0.00943805180225747	0.9234368947863348
>500	0.001115837664649332	0.4158274385578263
>1k	4.0575915078157484E-4	0.2958506619182171
>5k	3.0431936308618113E-4	1.2157748981472383
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9676	0.29468321554329097	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9086	0.2767147267906513	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5011	0.15261033413470762	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0455065682440158E-5
2	0.0	0.0	0.0	0.0	3.0455065682440158E-5
3	0.0	0.0	0.0	0.0	3.0455065682440158E-5
4	0.0	0.0	0.0	0.0	3.0455065682440158E-5
5	0.0	0.0	0.0	0.0	9.136519704732047E-5
6	0.0	0.0	0.0	0.0	9.136519704732047E-5
7	0.0	0.0	0.0	0.0	9.136519704732047E-5
8	0.0	0.0	0.0	0.0	9.136519704732047E-5
9	0.0	0.0	0.0	0.0	9.136519704732047E-5
10-11	0.0	0.0	0.0	0.0	1.5227532841220078E-4
12-13	0.0	0.0	0.0	0.0	3.350057225068417E-4
14-15	0.0	0.0	0.0	0.0	4.568259852366024E-4
16-17	0.0	0.0	0.0	0.0	6.243288464900232E-4
18-19	0.0	0.0	0.0	0.0	7.918317077434441E-4
20-21	0.0	0.0	0.0	1.5227532841220079E-5	9.59334568996865E-4
22-23	0.0	0.0	0.0	1.6750286125342089E-4	9.897896346793052E-4
24-25	0.0	0.0	0.0	4.568259852366024E-4	0.0010050171675205252
26-27	0.0	0.0	0.0	0.0011877475616151661	0.0010506997660441855
28-29	0.0	0.0	0.0	0.004233254129859182	0.0010963823645678457
30-31	0.0	0.0	0.0	0.012699762389577545	0.0010963823645678457
32-33	0.0	0.0	0.0	0.035799929709708404	0.001157292495932726
34-35	0.0	0.0	0.0	0.07281806204671441	0.0012182026272976063
36-37	0.0	0.0	0.0	0.1308958723031278	0.0012182026272976063
38-39	0.0	0.0	0.0	0.2193982931762989	0.0013095678243449267
40-41	0.0	0.0	0.0	0.31700677868851956	0.0013400228900273669
42-43	0.0	0.0	0.0	0.4172039447837477	0.001370477955709807
44-45	0.0	0.0	0.0	0.5455263640367093	0.0014161605542334674
46-47	0.0	0.0	0.0	0.7024613174983234	0.0014618431527571275
48-49	0.0	0.0	0.0	0.8764511077421041	0.001537980816963228
50-51	0.0	0.0	0.0	1.0577653412825114	0.0016141184811693284
52-53	0.0	0.0	0.0	1.2380136475240335	0.001735938743899089
54-55	0.0	0.0	0.0	1.4388648056997266	0.0017511662767403092
56-57	0.0	0.0	0.0	1.6801602911017	0.0018273039409464095
58-59	0.0	0.0	0.0	1.9570882033521282	0.0019034416051525098
60-61	0.0	0.0	0.0	2.244431748065951	0.00197957926935861
62-63	0.0	0.0	0.0	2.5371506118727245	0.0020404894007234903
64-65	0.0	0.0	0.0	2.8516600751752845	0.0020404894007234903
66-67	0.0	0.0	0.0	3.20743615247755	0.0020709444664059307
68-69	0.0	0.0	0.0	3.608179134259939	0.0020709444664059307
70-71	0.0	0.0	0.0	4.035753028908557	0.0020709444664059307
72-73	0.0	0.0	0.0	4.47948333590171	0.002131854597770811
74-75	0.0	0.0	0.0	4.921188381026981	0.0022232197948181317
76-77	0.0	0.0	0.0	5.413799068440451	0.0022536748605005716
78-79	0.0	0.0	0.0	5.955975375252092	0.002284129926183012
80-81	0.0	0.0	0.0	6.538108728239094	0.002284129926183012
82-83	0.0	0.0	0.0	7.139718095730017	0.0023602675903891123
84-85	0.0	0.0	0.0	7.741175187892528	0.0024059501889127726
86-87	0.0	0.0	0.0	8.360296218150854	0.0025125429188013132
88	0.0	0.0	0.0	8.847592496602738	0.0026191356486898534
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6130	0.0	57.316986	1
GTATCAA	11615	0.0	44.54477	1
CAACGCA	14290	0.0	35.389343	5
ATCAACG	14305	0.0	35.344673	3
TATCAAC	14945	0.0	34.367134	2
AACGCAG	15735	0.0	32.79604	6
TCAACGC	15910	0.0	31.926918	4
ACGCAGA	16800	0.0	30.157042	7
CGCAGAG	17035	0.0	29.685839	8
GTGGTAT	1865	0.0	27.237558	1
CGCGTTG	70	0.00884022	26.855988	7
GCAGAGT	18845	0.0	26.634695	9
GAGTACT	12565	0.0	23.882017	12-13
CGTCGAA	100	0.001752651	23.515465	94
GTACATG	10825	0.0	22.985344	1
CAGAGTA	18345	0.0	22.877382	10-11
AGAGTAC	17485	0.0	22.712433	10-11
TGGTATC	2295	0.0	22.33883	2
ACATGGG	10535	0.0	22.27765	3
TACATGG	10870	0.0	22.19752	2
>>END_MODULE