FastQCFastQC Report
Mon 16 Jan 2017
ERR1146616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4921287
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51200.033.7971761
GTATCAA74150.032.4050181
ATCAACG78500.028.2671553
CAACGCA78250.028.2364445
AACGCAG89800.025.3819486
GTACTAG22850.025.3117081
GTACATG159800.025.247191
TATCAAC95200.024.7899992
TACATGG156800.024.4055212
TCAACGC91550.024.340024
ACATGGG162550.022.8191683
ACGCAGA97100.022.795447
CGCAGAG101150.021.9291828
CTAACGC10000.020.685333
CATGGGG125050.020.6014484
CTAGTAC29950.020.4059263
GCTATTG29650.020.1291458
TCTAACG10750.020.116812
GGGCTAT32750.019.5158376
GCATCGT24050.019.3448988
Produced by FastQC (version 0.11.5)